CN101815789B - 靶序列的富集 - Google Patents

靶序列的富集 Download PDF

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Publication number
CN101815789B
CN101815789B CN2008801098230A CN200880109823A CN101815789B CN 101815789 B CN101815789 B CN 101815789B CN 2008801098230 A CN2008801098230 A CN 2008801098230A CN 200880109823 A CN200880109823 A CN 200880109823A CN 101815789 B CN101815789 B CN 101815789B
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sequence
target
pcr
temperature
target sequence
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CN101815789A (zh
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G·马克里吉奥古斯
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Dana Farber Cancer Institute Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2008801098230A 2007-08-01 2008-07-31 靶序列的富集 Active CN101815789B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96283807P 2007-08-01 2007-08-01
US60/962,838 2007-08-01
PCT/US2008/009248 WO2009017784A2 (en) 2007-08-01 2008-07-31 Enrichment of a target sequence

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CN101815789A CN101815789A (zh) 2010-08-25
CN101815789B true CN101815789B (zh) 2013-06-12

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US (1) US8455190B2 (https=)
EP (1) EP2183379B1 (https=)
JP (1) JP5531367B2 (https=)
KR (1) KR101376359B1 (https=)
CN (1) CN101815789B (https=)
AU (1) AU2008282780B2 (https=)
CA (1) CA2695264C (https=)
DK (1) DK2183379T3 (https=)
ES (1) ES2547053T3 (https=)
PT (1) PT2183379E (https=)
WO (1) WO2009017784A2 (https=)

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Also Published As

Publication number Publication date
EP2183379A4 (en) 2011-08-10
DK2183379T3 (en) 2015-07-27
JP2010535031A (ja) 2010-11-18
CN101815789A (zh) 2010-08-25
US20100203532A1 (en) 2010-08-12
JP5531367B2 (ja) 2014-06-25
KR20100044870A (ko) 2010-04-30
WO2009017784A3 (en) 2009-03-26
AU2008282780B2 (en) 2014-04-17
ES2547053T3 (es) 2015-10-01
CA2695264A1 (en) 2009-02-05
EP2183379A2 (en) 2010-05-12
AU2008282780A1 (en) 2009-02-05
PT2183379E (pt) 2015-09-25
US8455190B2 (en) 2013-06-04
EP2183379B1 (en) 2015-04-15
KR101376359B1 (ko) 2014-03-27
WO2009017784A2 (en) 2009-02-05
CA2695264C (en) 2015-01-27

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