AU2008282780B2 - Enrichment of a target sequence - Google Patents

Enrichment of a target sequence Download PDF

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Publication number
AU2008282780B2
AU2008282780B2 AU2008282780A AU2008282780A AU2008282780B2 AU 2008282780 B2 AU2008282780 B2 AU 2008282780B2 AU 2008282780 A AU2008282780 A AU 2008282780A AU 2008282780 A AU2008282780 A AU 2008282780A AU 2008282780 B2 AU2008282780 B2 AU 2008282780B2
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AU
Australia
Prior art keywords
target
sequence
pcr
temperature
sequences
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
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AU2008282780A
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AU2008282780A1 (en
Inventor
Gerassimos Makrigiorgos
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Dana Farber Cancer Institute Inc
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Dana Farber Cancer Institute Inc
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Publication of AU2008282780A1 publication Critical patent/AU2008282780A1/en
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Ceased legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2008282780A 2007-08-01 2008-07-31 Enrichment of a target sequence Ceased AU2008282780B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96283807P 2007-08-01 2007-08-01
US60/962,838 2007-08-01
PCT/US2008/009248 WO2009017784A2 (en) 2007-08-01 2008-07-31 Enrichment of a target sequence

Publications (2)

Publication Number Publication Date
AU2008282780A1 AU2008282780A1 (en) 2009-02-05
AU2008282780B2 true AU2008282780B2 (en) 2014-04-17

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ID=40305136

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2008282780A Ceased AU2008282780B2 (en) 2007-08-01 2008-07-31 Enrichment of a target sequence

Country Status (11)

Country Link
US (1) US8455190B2 (https=)
EP (1) EP2183379B1 (https=)
JP (1) JP5531367B2 (https=)
KR (1) KR101376359B1 (https=)
CN (1) CN101815789B (https=)
AU (1) AU2008282780B2 (https=)
CA (1) CA2695264C (https=)
DK (1) DK2183379T3 (https=)
ES (1) ES2547053T3 (https=)
PT (1) PT2183379E (https=)
WO (1) WO2009017784A2 (https=)

Families Citing this family (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US8980561B1 (en) 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
WO2008105814A2 (en) 2006-08-22 2008-09-04 Los Alamos National Security, Llc Miniturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
EP2140033B1 (en) 2007-03-28 2012-10-10 Signal Diagnostics System and method for high resolution analysis of nucleic acids to detect sequence variations
EP2183379B1 (en) 2007-08-01 2015-04-15 Dana Farber Cancer Institute Enrichment of a target sequence
EP3067694A1 (en) 2008-05-05 2016-09-14 Los Alamos National Security, LLC Lateral flow-based nucleic acid sample preparation device, integrated with passive fluid flow control
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
CN102859003B (zh) 2010-03-08 2014-07-30 达纳-法伯癌症研究院有限公司 使用参考封闭序列的全cold-pcr富集
KR101312241B1 (ko) * 2010-04-27 2013-09-27 사회복지법인 삼성생명공익재단 증폭억제시발체를 이용하는 유전자 돌연변이 검출 방법
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US12545960B2 (en) 2010-05-18 2026-02-10 Natera, Inc. Methods for simultaneous amplification of target loci
EP2572003A4 (en) 2010-05-18 2016-01-13 Natera Inc METHOD FOR NONINVASIVE PRANATAL PLOIDIE ASSIGNMENT
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
WO2012088456A2 (en) 2010-12-22 2012-06-28 Natera, Inc. Methods for non-invasive prenatal paternity testing
AU2011358564B9 (en) 2011-02-09 2017-07-13 Natera, Inc Methods for non-invasive prenatal ploidy calling
AU2012223438A1 (en) * 2011-02-28 2013-09-26 Transgenomic, Inc Kit and method for sequencing a target DNA in a mixed population
ITTO20110271A1 (it) 2011-03-28 2012-09-29 Fabio Gentilini Metodo per l'identificazione di polimorfismi genetici di base singola.
EP3333269B1 (en) * 2011-03-31 2021-05-05 Dana-Farber Cancer Institute, Inc. Methods to enable multiplex cold-pcr
US10519492B2 (en) 2011-04-20 2019-12-31 Mesa Biotech, Inc. Integrated device for nucleic acid detection and identification
US9796748B2 (en) 2011-05-02 2017-10-24 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
EP2758458A4 (en) 2011-07-10 2015-10-21 Harvard College COMPOSITIONS AND METHODS FOR SELF-ASSEMBLING POLYMERS WITH COMPLEMENTARY MACROSCOPIC AND MICROSCOPIC SCALE UNITS
WO2013010074A1 (en) 2011-07-13 2013-01-17 Primeradx, Inc. Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample
US9074247B2 (en) * 2011-10-06 2015-07-07 Drexel University P53 assay for a urine test for HCC screening
CN103998921B (zh) * 2011-10-14 2016-04-20 贝克顿·迪金森公司 方波热循环
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
WO2013188840A1 (en) * 2012-06-14 2013-12-19 Fred Hutchinson Cancer Research Center Compositions and methods for sensitive mutation detection in nucleic acid molecules
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US9732390B2 (en) 2012-09-20 2017-08-15 The Chinese University Of Hong Kong Non-invasive determination of methylome of fetus or tumor from plasma
US10706957B2 (en) 2012-09-20 2020-07-07 The Chinese University Of Hong Kong Non-invasive determination of methylome of tumor from plasma
US10913977B2 (en) * 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
RU2016117929A (ru) 2013-10-09 2017-11-15 Флюоресентрик, Инк. Мультиплексные зонды
CA2922261A1 (en) * 2013-10-20 2015-05-21 Trovagene, Inc. Synthesis and enrichment of nucleic acid sequences
CN105283555A (zh) 2013-10-20 2016-01-27 特罗瓦基因公司 核酸序列的合成和富集
CN103602748A (zh) * 2013-11-28 2014-02-26 瑞希基因科技（北京）股份有限公司 一种结合荧光定量pcr技术的焦磷酸测序方法
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
EP3957749A1 (en) 2014-04-21 2022-02-23 Natera, Inc. Detecting tumour specific mutations in biopsies with whole exome sequencing and in cell-free samples
US20180173845A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
CA2954420A1 (en) * 2014-07-10 2016-01-14 Fluoresentric, Inc. Dna amplification technology
ES2741400T3 (es) 2014-07-18 2020-02-10 Univ Hong Kong Chinese Análisis de patrones de metilación de tejidos en mezcla de ADN
US20160310948A1 (en) 2015-04-24 2016-10-27 Mesa Biotech, Inc. Fluidic Test Cassette
WO2016183106A1 (en) 2015-05-11 2016-11-17 Natera, Inc. Methods and compositions for determining ploidy
LT3298162T (lt) 2015-05-18 2020-03-10 Saga Diagnostics Ab Tikslinės nukleorūgšties ir variantų aptikimas
EP4435112A3 (en) * 2015-05-27 2025-02-26 Quest Diagnostics Investments Incorporated Compositions and methods for screening solid tumors
WO2017070339A1 (en) * 2015-10-20 2017-04-27 Richardson Katherine Microfluidic device for enrichment of nucleic acid sequence alterations
WO2017181202A2 (en) 2016-04-15 2017-10-19 Natera, Inc. Methods for lung cancer detection
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
WO2018099418A1 (en) 2016-11-30 2018-06-07 The Chinese University Of Hong Kong Analysis of cell-free dna in urine and other samples
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US11371090B2 (en) 2016-12-12 2022-06-28 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
JP2021506342A (ja) 2017-12-14 2021-02-22 ティーエーアイ ダイアグノスティックス インコーポレイテッドＴａｉ Ｄｉａｇｎｏｓｔｉｃｓ，Ｉｎｃ． 移植のための移植片適合性の評価
CN119753091A (zh) 2018-04-02 2025-04-04 格里尔公司 用于扩增与癌症相关联的cfDNA分子的化验板
CA3090426A1 (en) 2018-04-14 2019-10-17 Natera, Inc. Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
JP6544783B1 (ja) 2018-07-31 2019-07-17 学校法人 岩手医科大学 がんの診断のためのプローブ／プライマーライブラリー
IL281741B2 (en) 2018-09-27 2026-04-01 Grail Inc Methylation markers and a targeted methylation detection panel
EP3980559A1 (en) 2019-06-06 2022-04-13 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
EP4017998B1 (en) 2019-08-21 2024-11-06 Dana-Farber Cancer Institute, Inc. Multi-site enrichment of deletions in dna microsatellites
US11866793B2 (en) 2020-03-27 2024-01-09 Regents Of The University Of Minnesota Assay for detecting palmer amaranth DNA in individual and mixed samples
AU2022486981A1 (en) 2022-11-22 2025-06-12 Quantdetect, Inc. Probe/primer library for diagnosis of cancer

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003072809A1 (en) * 2002-02-26 2003-09-04 Commonwealth Scientific And Industrial Research Organisation Melting temperature dependent dna amplification
US20060063175A1 (en) * 2002-12-18 2006-03-23 Dingbang Xu Method of polymerase chain reaction with ultra-low denaturing temperatures and applications thereof
US20070154892A1 (en) * 2005-12-30 2007-07-05 Simon Wain-Hobson Differential amplification of mutant nucleic acids by PCR in a mixure of nucleic acids

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5310652A (en) * 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
GB2228086A (en) 1988-11-25 1990-08-15 Ici Plc Characterisation of genomic DNA
KR920700360A (ko) 1989-03-22 1992-02-19 하리크 프리드리히 미끄럼 베어링
US5075217A (en) * 1989-04-21 1991-12-24 Marshfield Clinic Length polymorphisms in (dC-dA)n ·(dG-dT)n sequences
WO1990013668A1 (en) 1989-05-05 1990-11-15 Lifecodes Corporation Method for genetic analysis of a nucleic acid sample
CA2020958C (en) * 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5045450A (en) * 1989-07-13 1991-09-03 Massachusetts Institute Of Technology Determination of a mutational spectrum
DE3938907C2 (de) * 1989-11-24 1999-11-04 Dade Behring Marburg Gmbh Mittel zum Lagern und Suspendieren von Zellen, insbesondere Erythrozyten
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
DE69118930T2 (de) * 1990-01-26 1997-01-09 Abbott Lab Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion
AU638568B2 (en) * 1990-02-26 1993-07-01 United States of America, as represented by the Secretary, U.S. Department of Commerce, The A method for the fluorescent detection of a dna sequence in real time
WO1991014003A2 (en) 1990-03-09 1991-09-19 Ig Laboratories, Inc. Characterization and analysis of polymorphic vntr loci
US5846710A (en) * 1990-11-02 1998-12-08 St. Louis University Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5631147A (en) * 1995-09-21 1997-05-20 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
AU714486B2 (en) 1995-11-21 2000-01-06 Yale University Unimolecular segment amplification and detection
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
JP3785517B2 (ja) * 1997-06-05 2006-06-14 東ソー株式会社 核酸融解温度測定法
AU9063398A (en) 1997-09-12 1999-04-05 Exiqon A/S Oligonucleotide analogues
US6174680B1 (en) * 1998-12-30 2001-01-16 Dana-Farber Cancer Institute, Inc. Method for identifying mismatch repair glycosylase reactive sites, compounds and uses thereof
US6850846B2 (en) * 2000-01-11 2005-02-01 Affymetrix, Inc. Computer software for genotyping analysis using pattern recognition
US6762049B2 (en) * 2001-07-05 2004-07-13 Institute Of Microelectronics Miniaturized multi-chamber thermal cycler for independent thermal multiplexing
CN100334223C (zh) * 2002-12-18 2007-08-29 徐定邦 一种超低变性温度的聚合酶链式反应方法及其应用
US7456281B2 (en) * 2005-04-20 2008-11-25 Idaho Technology, Inc. Nucleic acid melting analysis with saturation dyes
US20070000020A1 (en) * 2005-06-30 2007-01-04 Kimberly-Clark Worldwide, Inc. Surgical glove with modified frictional interface and method for producing same
EP2171097A2 (en) * 2007-06-29 2010-04-07 Population Genetics Technologies LTD. Methods and compositions for isolating nucleic acid sequence variants
EP2183379B1 (en) 2007-08-01 2015-04-15 Dana Farber Cancer Institute Enrichment of a target sequence
US8071338B2 (en) 2007-08-08 2011-12-06 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003072809A1 (en) * 2002-02-26 2003-09-04 Commonwealth Scientific And Industrial Research Organisation Melting temperature dependent dna amplification
US20060063175A1 (en) * 2002-12-18 2006-03-23 Dingbang Xu Method of polymerase chain reaction with ultra-low denaturing temperatures and applications thereof
US20070154892A1 (en) * 2005-12-30 2007-07-05 Simon Wain-Hobson Differential amplification of mutant nucleic acids by PCR in a mixure of nucleic acids

Also Published As

Publication number Publication date
EP2183379A4 (en) 2011-08-10
DK2183379T3 (en) 2015-07-27
JP2010535031A (ja) 2010-11-18
CN101815789B (zh) 2013-06-12
CN101815789A (zh) 2010-08-25
US20100203532A1 (en) 2010-08-12
JP5531367B2 (ja) 2014-06-25
KR20100044870A (ko) 2010-04-30
WO2009017784A3 (en) 2009-03-26
ES2547053T3 (es) 2015-10-01
CA2695264A1 (en) 2009-02-05
EP2183379A2 (en) 2010-05-12
AU2008282780A1 (en) 2009-02-05
PT2183379E (pt) 2015-09-25
US8455190B2 (en) 2013-06-04
EP2183379B1 (en) 2015-04-15
KR101376359B1 (ko) 2014-03-27
WO2009017784A2 (en) 2009-02-05
CA2695264C (en) 2015-01-27

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