CN101695272A - Natural reproduction method for dendrobium - Google Patents
Natural reproduction method for dendrobium Download PDFInfo
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- CN101695272A CN101695272A CN200910191211A CN200910191211A CN101695272A CN 101695272 A CN101695272 A CN 101695272A CN 200910191211 A CN200910191211 A CN 200910191211A CN 200910191211 A CN200910191211 A CN 200910191211A CN 101695272 A CN101695272 A CN 101695272A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention discloses a natural reproduction method for dendrobium, which comprises the following steps of: preparing a natural reproduction seed bed, controlling a preset reproduction environment, culturing seeds, managing intertill and transplanting seedlings. The method reduces the cost of fast young plants, gives play to the advantage of a large amount of fruit seeds of dendrobium and compensates for the disadvantages of thin seed embryo, incomplete growth and no endosperm. By using the modern facility cultivation technology, the method comprehensively adjusts and controls the most proper environmental factors of the dendrobium, thereby increasing the germination rate of seeds and promoting growth of plants so as to realize cultivating the seed embryo outside test tubes from the prior method for cultivating the seed embryo in test tubes, wherein the germination rate of seeds reaches over 6 percent.
Description
Technical field
The present invention relates to a kind of stem of noble dendrobium propagation method, further relate to a kind of seed natural propagation method of the stem of noble dendrobium.
Technical background
Dendrobium (Dendrobium) is the second largest genus of the orchid family, and the whole world has 1500 kinds approximately, and there are 74 kinds of 2 mutation in China, but wherein hyoscine kind more than 40 arranged, mainly be distributed in the south China and the southwest of China.Wherein, mainly contain dendrobium candidum, purple dendrobium, HERBA DENDROBII, dendrobium devonianum, Dendrobidium huoshanness, dendrobium; The stem of noble dendrobium is a rare traditional Chinese medicine, in China long history is arranged, and the medicinal beginning is stated from Shennong's Herbal, classifies as top gradely, and " it is sweet to distinguish the flavor of, flat.In main the wound, remove numbness, the therapeutic method to keep the adverse qi flowing downward, the tonifying five zang organs consumptive disease is won thin, reinforcing yin essence.The thick stomach of clothes is made light of one's life by commiting suicide and is prolonged life for a long time." its active ingredient mainly is dendrobine and polysaccharide, is widely used in drinks such as being processed into health products, medicine and stem of noble dendrobium soup, dendrobium wine at present.Medicinal plant cultivation is the most direct, the effective means of protection, expansion, regeneration resources of medicinal plant.The Dendrobium plant is had relatively high expectations to ecological condition, and the cultivation difficulty is big, and its area under cultivation and scale are very limited.Study intensively for many years through domestic scientific research institutions and colleges and universities, dendrobium candidum, HERBA DENDROBII, dendrobium moniliformeSweet etc. have been obtained remarkable achievement from the tissue culture to the field production, culture technique is succeedd, but all there are the too high problem of seedling cost in cuttage, group training and non-test tube rapid propagation.Very small of stem of noble dendrobium seed volume comprises more than 10000000 seed in the kind fruit, even but under the wild condition of the best, the survival rate of seed is lower than 2%, and at present, survival rate does not break through 1% under the artificial condition.Chinese patent application (200610048891.3) discloses a kind of cultivation method of dendrobium candidum seed; Chinese patent application (200910036502.9) discloses a kind of efficient propagation method that uses dendrobium candidum seed growing seedling; But said method all is based on the basis of test tube rapid propagation; The cost height, difficulty is big.At present, artificial seed is bred the stem of noble dendrobium and is not also broken through the problem that survival rate is low, breeding condition is harsh under field conditions (factors).
Summary of the invention
The invention provides a kind of seed natural propagation method method of the stem of noble dendrobium, comprise the control of the preparation in simulating nature breeding seedbed, default breeding environment, cultivation, intertillage management and the seedling replanting of seed, the culture matrix in natural propagation seedbed is the female matrix of forest land type fungi and floc by mass ratio is 1: 1～3 to be mixed and made into; Temp. and humidity and illumination condition are that temperature is 10～20 ℃, 34.20 ℃ of the highest extreme temperatures, and 0 ℃ of minimum extreme temperature, average relative humidity 70～90%, intensity of illumination 10000 ~ 5000lx, average daily light application time is 8～14 hours.
The preparation method of the female matrix of described forest land type fungi is:
A, carry the cultivation of female matrix of symbiosis fungi: screening stem of noble dendrobium wild seed germination rate is higher than 1% phytodet ritus for cultivating material, to cultivate material pulverizes, press 5～10 centimetres of planes of thickness and pile up cultivation, cultivating humidity is 70～100%, cultivation temperature is 15～30 ℃, incubation time 1～7 month obtains carrying female matrix of symbiosis fungi;
The enlarged culture of the female matrix of B, forest land type fungi: it is numerous that the female matrix that carries the symbiosis fungi and described phytodet ritus mix the expansion that can carry out female matrix by 1: 1～3, and expanding numerous time is 0.5～3 month, obtains forest land type fungi mother matrix.
The withered bark top layer of described phytodet ritus softwood forest, broad leaved forest or hillside type shrub or the bark rot of trunk lower end.
Described floc is a sawdust: the wood chip mass ratio is to be mixed and made at 1～3: 1～3, and humidity is 50～90%.
The seedbed of simulating nature breeding is to utilize the flaw-piece waste material or the asbestos shingle of Wood Processing to do the end, 10～15 centimetres of the height of bed, 1～1.2 meter of bedside, 0.8～1.0 meter on support; The female matrix of forest land type fungi is laid on the seedbed 5～10 centimetres of thickness.
The cultivation of seed is:
I, selection completely filled fruit, 100% maturation and the fruit that does not ftracture or just ftractureed are to plant really;
II, embryo are put into water, vibration mixes, and each plants embryo water 1000～10000mL dilution of fruit, uses atomizers spray on the culture matrix in natural propagation seedbed, be spread on the liver moss of pulverizing seed is covered, under described temp. and humidity and illumination condition, carry out seed cultivation.
The intertillage management is filled a prescription to such an extent that nutrient solution sprays with the desaccharification of full-valence nutrition liquor, 1/2MS or the MS of soilless culture, and the spraying method of nutrient solution and water is the atomizing mode, and field management should keep not having the weeds state.
When being long 3～5 centimetres of seedling, seedling replanting transplants.
Useful situation effect of the present invention is: the present invention has overcome problems such as complex procedures that planting techniques such as test tube tissue rapid propagation and cuttage exist, seedling cost height, performance stem of noble dendrobium fruit and seed amount big advantage, by nutrient solution spray with micro-ecological environment that control remedies that embryo is tiny, ateliosis and exalbuminose shortcoming; Rely on the suitableeest environmental factor of modern installations culture technique comprehensive regulation stem of noble dendrobium growth, seed germination rate has reached more than 6%, promotes plant strain growth.
Embodiment
The bark top layer or the humus in the higher plot of screening wild seed germination rate are pulverized, and keep soil humidity 70～100%, 15～30 ℃ of temperature, 5～10 centimetres of cultivations of thickness, as the female matrix that carries the symbiosis fungi.It is numerous that female matrix and humus mix the expansion that can carry out female matrix by 1: 1～3.Female matrix, sawdust, wood chip are mixed by 1: 0.5～1.5: 0.5～1.5, as the matrix of seed natural propagation technology.
Under facilities such as minimum facility, booth, greenhouse, carry out, decide as the case may be, guarantee ventilated, have abundant water resources, temperature is controlled at 15～30 ℃, humidity control 70～90%.Green house should have full-automatic spraying facility, fan, heat facility or air-conditioning.
Perhaps, it is moistening to select to have a moderate climate, and belongs to the torrid zone, the warm and humid type weather in subtropics, 10～20 ℃ of year-round average temperatures; Extreme temperature was 34.20 ℃ in the hottest month, and extreme temperature was 0 ℃ in the coldest month; Average relative humidity 70～90%; Ventilated place is built little minimum facility and can be cultivated.
Utilize the flaw-piece waste material or the asbestos shingle of Wood Processing to do the end, it is long because of ground is decided, the implant bed of 0.8～1.0 meter on support to make 10～15 centimetres of the height of bed, bedside 1～1.2 meter, bed.Matrix is laid on the seedbed 5～10 centimetres of thickness
Select completely filled fruit, 100% ripe and not cracking or the just fruit of cracking takes out embryo in the fruit; Pulverous embryo is put into water, vibration mixes, 1000～10000mL water applies the embryo in 1 fruit,, is spread on the matrix that is sprayed with seed with the liver moss of pulverizing in cultivating on the matrix with atomizers spray, carries out seed cultivation.
The nurturing period temperature is controlled at 15～30 ℃, humidity control 70～90%.The full-valence nutrition liquor of nutrient solution employing soilless culture or 1/2MS, MS desaccharification prescription are cultivated.Spray nutritious liquor or moisture adopt the mode of atomizing.The seed germination primary growth is slow, and Ying Jiancao promptly pulls out.
Treat to transplant when seedling grows to 3～5 centimetres.
Claims (8)
1. the seed natural propagation method of a stem of noble dendrobium, comprise the control of the preparation in simulating nature breeding seedbed, default breeding environment, cultivation, intertillage management and the seedling replanting of seed, it is characterized in that: the culture matrix in natural propagation seedbed is the female matrix of forest land type fungi and floc by mass ratio is 1: 1～3 to be mixed and made into; Temp. and humidity and illumination condition are that temperature is 10～20 ℃, 34.20 ℃ of the highest extreme temperatures, and 0 ℃ of minimum extreme temperature, average relative humidity 70～90%, intensity of illumination 10000～5000lx, average daily light application time is 8～14 hours.
2. the seed natural propagation method of the stem of noble dendrobium according to claim 1 is characterized in that: the preparation method of the female matrix of described forest land type fungi is:
A, carry the cultivation of female matrix of symbiosis fungi: screening stem of noble dendrobium wild seed germination rate is higher than 1% phytodet ritus for cultivating material, to cultivate material pulverizes, press 5～10 centimetres of planes of thickness and pile up cultivation, cultivating humidity is 70～100%, cultivation temperature is 15～30 ℃, incubation time 1～7 month obtains carrying female matrix of symbiosis fungi;
The enlarged culture of the female matrix of B, forest land type fungi: it is numerous that the female matrix that carries the symbiosis fungi and described phytodet ritus mix the expansion that can carry out female matrix by 1: 1～3, and expanding numerous time is 0.5～3 month, obtains forest land type fungi mother matrix.
3. the seed natural propagation method of the stem of noble dendrobium according to claim 2 is characterized in that: the withered bark top layer of described phytodet ritus softwood forest, broad leaved forest or hillside type shrub or the bark rot of trunk lower end.
4. the seed natural propagation method of the stem of noble dendrobium according to claim 1, it is characterized in that: described floc is a sawdust: the wood chip mass ratio is to be mixed and made at 1～3: 1～3, and humidity is 50～90%.
5. the seed natural propagation method of the stem of noble dendrobium according to claim 1 is characterized in that: the seedbed of simulating nature breeding is to utilize the flaw-piece waste material or the asbestos shingle of Wood Processing to do the end, 10～15 centimetres of the height of bed, 1～1.2 meter of bedside, 0.8～1.0 meter on support; The female matrix of forest land type fungi is laid on the seedbed 5～10 centimetres of thickness.
6. the seed natural propagation method of the stem of noble dendrobium according to claim 1, it is characterized in that: the cultivation of seed is:
I, selection completely filled fruit, 100% maturation and the fruit that does not ftracture or just ftractureed are to plant really;
II, embryo are put into water, vibration mixes, and each plants embryo water 1000～10000mL dilution of fruit, uses atomizers spray on the culture matrix in natural propagation seedbed, be spread on the liver moss of pulverizing seed is covered, under described temp. and humidity and illumination condition, carry out seed cultivation.
7. the seed natural propagation method of the stem of noble dendrobium according to claim 1, it is characterized in that: the intertillage management is filled a prescription to such an extent that nutrient solution sprays with the desaccharification of full-valence nutrition liquor, 1/2MS or the MS of soilless culture, the spraying method of nutrient solution and water is the atomizing mode, and field management should keep not having the weeds state.
8. the seed natural propagation method of the stem of noble dendrobium according to claim 1 is characterized in that: transplant when seedling replanting is long 3～5 centimetres an of seedling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2009101912117A CN101695272B (en) | 2009-10-26 | 2009-10-26 | Natural reproduction method for dendrobium |
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| CN2009101912117A CN101695272B (en) | 2009-10-26 | 2009-10-26 | Natural reproduction method for dendrobium |
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| CN101695272A true CN101695272A (en) | 2010-04-21 |
| CN101695272B CN101695272B (en) | 2011-03-30 |
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| CN2009101912117A Expired - Fee Related CN101695272B (en) | 2009-10-26 | 2009-10-26 | Natural reproduction method for dendrobium |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102440130A (en) * | 2011-09-22 | 2012-05-09 | 澄思源生物科技（上海）有限公司 | Method for natural propagation of dendrobium officinale seeds |
| CN103004445A (en) * | 2013-01-06 | 2013-04-03 | 戴亚峰 | Method for directly seedling dendrobe seedlings by using plastic greenhouse |
| CN103798013A (en) * | 2013-12-23 | 2014-05-21 | 茂名市惠生源生物科技有限公司 | Cultivating method for dendrobium officinale seed seedlings |
| CN103931410A (en) * | 2014-05-06 | 2014-07-23 | 龙陵县云河石斛开发有限责任公司 | Open forest seedling cultivation method for Den. devonianum Paxt |
| CN104094767A (en) * | 2014-07-31 | 2014-10-15 | 张松波 | Dendrobium officinale planting method |
| CN105724209A (en) * | 2016-02-22 | 2016-07-06 | 云南兰京农业发展有限责任公司 | Novel dendrobe culture medium |
| CN106577232A (en) * | 2016-12-13 | 2017-04-26 | 重庆市中药研究院 | Natural propagation method for sowing seeds of dendrobium flexicaule on palisades |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1157107C (en) * | 2002-09-17 | 2004-07-14 | 华南师范大学 | Fast propagating method of Dendrobium nobile Lindl |
| CN1675991A (en) * | 2004-04-01 | 2005-10-05 | 寿伟康 | Method for culturing iron-sheet dendrobe |
| CN1284448C (en) * | 2004-12-29 | 2006-11-15 | 中国科学院华南植物园 | High quality germchit tissure culturing and rapid breeding method of Dendrobium sp. |
| CN100586275C (en) * | 2008-01-18 | 2010-02-03 | 中国科学院昆明植物研究所 | Fast replication method for dendrobium |
| CN101248729A (en) * | 2008-03-31 | 2008-08-27 | 中国林业科学研究院林业研究所 | Spring dendrobium stem good quality completed product breeding flower method |
| CN101336589A (en) * | 2008-08-13 | 2009-01-07 | 吴江市苗圃集团有限公司 | Cottage breeding method of dendrobium officinale |
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2009
- 2009-10-26 CN CN2009101912117A patent/CN101695272B/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102440130A (en) * | 2011-09-22 | 2012-05-09 | 澄思源生物科技（上海）有限公司 | Method for natural propagation of dendrobium officinale seeds |
| CN103004445A (en) * | 2013-01-06 | 2013-04-03 | 戴亚峰 | Method for directly seedling dendrobe seedlings by using plastic greenhouse |
| CN103798013A (en) * | 2013-12-23 | 2014-05-21 | 茂名市惠生源生物科技有限公司 | Cultivating method for dendrobium officinale seed seedlings |
| CN103931410A (en) * | 2014-05-06 | 2014-07-23 | 龙陵县云河石斛开发有限责任公司 | Open forest seedling cultivation method for Den. devonianum Paxt |
| CN104094767A (en) * | 2014-07-31 | 2014-10-15 | 张松波 | Dendrobium officinale planting method |
| CN105724209A (en) * | 2016-02-22 | 2016-07-06 | 云南兰京农业发展有限责任公司 | Novel dendrobe culture medium |
| CN106577232A (en) * | 2016-12-13 | 2017-04-26 | 重庆市中药研究院 | Natural propagation method for sowing seeds of dendrobium flexicaule on palisades |
| CN106577232B (en) * | 2016-12-13 | 2020-07-24 | 重庆市中药研究院 | Natural propagation method for rock wall sowing of dendrobium aspergillum seeds |
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| CN101695272B (en) | 2011-03-30 |
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