CN101688195A - 制备重组人凝血酶’644的方法 - Google Patents
制备重组人凝血酶’644的方法 Download PDFInfo
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- CN101688195A CN101688195A CN200880023664A CN200880023664A CN101688195A CN 101688195 A CN101688195 A CN 101688195A CN 200880023664 A CN200880023664 A CN 200880023664A CN 200880023664 A CN200880023664 A CN 200880023664A CN 101688195 A CN101688195 A CN 101688195A
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- ecarin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
- C12N9/6418—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals from snakes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6429—Thrombin (3.4.21.5)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21005—Thrombin (3.4.21.5)
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Diabetes (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
蛋白 | CDNAEMBLACC# | 剪接变体(蛋白) | 突变 | GENEEMBLACC# |
谷氨酸-γ-羧化酶 | BC013979 | 2;BC013979;AF253530 | 1SNP(EMBL#U65896);2SNPs(OMIM#137167) | U65896 |
凝血酶原 | V00595 | 1;V00595 | 约100SNP′s(EMBL#AF478696) | AF478696 |
种 | 数据库登记号#/ID |
智人(Homo sapiens)(人) | NM_000821.2HUMGLUCARBHUMHGCABC004422HSU65896AF253530.1 |
Papio hamadryas(红狒狒) | AC116665.1 |
白鲸(Delphinapterus leucas)(白鲸) | AF278713 |
牛(Bos taurus)(牛) | NM_174066.2BOVCARBOXGBOVBGCA |
绵羊(Ovis aries)(家绵羊) | AF312035 |
褐鼠(Rattus norvegicus)(褐鼠) | NM_031756.1AF065387 |
小家鼠(Mus musculus)(小鼠) | NM_019802.1AF087938 |
蟾鱼(Opsanus tau)(硬骨鱼) | AF278714.1 |
织锦芋螺(Conus textile)(软体动物) | AY0044904.1 |
AF382823.2 | |
堂皇芋螺(Conus imperialis)(软体动物) | AF448234.1 |
Conus episcopatus(软体动物) | AF448233.1 |
Conus omaria(软体动物) | AF448235.1 |
黑腹果蝇(Drosophila melanogaster)(果蝇) | NM_079161.2 |
冈比亚按蚊(Anopheles gambiae)(蚊) | XM_316389.1 |
黑麦(Secale cereale)(单子叶植物) | SCE314767 |
小麦(Triticum aestivum)(普通小麦) | AF280606.1 |
Triticum urartu(单子叶植物) | AY245579.1 |
大麦(Hordeum vulgare)(大麦) | BLYHORDCA |
问号钩端螺旋体(Leptospira interrogans)(螺旋体) | AE011514.1 |
天蓝色链霉菌(Streptomyces coelicolor)(高GC革兰氏阳性菌) | SCO939109SCO939124AF425987.1 |
变铅青链霉菌(Streptomyces lividans)(高GC革兰氏阳性菌) | SLU22894 |
Streptomyces viginiae(高GC革兰氏阳性菌) | SVSNBDE |
藤黄微球菌(Micrococcus luteus)(高GC革兰氏阳性菌) | MLSPCOPER |
雷氏衣藻(Chlamydomonas reinhardtii)(绿藻) | AF479588.1 |
盘基网柄菌(Dictyostelium discoideum)(粘菌) | AC115612.2 |
鹌鹑(Coturnix coturnix)(鸟类) | AF364329.1 |
慢生大豆根瘤菌(Bradyrhizobium.japonicum)(α-proteobacteria) | AP005937.1 |
类球红细菌(Rhodobacter sphaeroides)(α-proteobacteria) | RSY14197 |
苜蓿中华根瘤菌(Sinorhizobium meliloti)(α-proteobacteria) | RME603647AF119834 |
Mesorhizobium loti(α-proteobacteria) | AP003014.2 |
青紫色素杆菌(Chromobacterium violaceum)(β-proteobacteria) | AE016910.1AE016918.1 |
铜绿假单胞菌(Pseudomonas aeruginosa)(γ-proteobacteria) | AE004613.1AF165882 |
地毯草黄单胞菌(Xanthomonas axonopodis)(γ-proteobacteria) | AE011706.1 |
人疱疹病毒8 | KSU52064 |
KSU75698AF305694AF360120AF192756 |
实验ID | 培养方法&时间 | 存活细胞(百万细胞/mL) | 凝血酶原mg/L |
CC2LC(272-8) | 分批,238h | 5.9 | 281 |
CC2LD(272-8) | 分批,238h | 6.2 | 276 |
326-11B | 灌流,259h | 18 | 722 |
Claims (16)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US94820707P | 2007-07-06 | 2007-07-06 | |
US60/948,207 | 2007-07-06 | ||
PCT/SE2008/050836 WO2009008821A1 (en) | 2007-07-06 | 2008-07-04 | Method for production of recombinant human thrombin `644 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101688195A true CN101688195A (zh) | 2010-03-31 |
CN101688195B CN101688195B (zh) | 2014-12-03 |
Family
ID=40228838
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200880023664.2A Expired - Fee Related CN101688195B (zh) | 2007-07-06 | 2008-07-04 | 制备重组人凝血酶‘644的方法 |
Country Status (8)
Country | Link |
---|---|
US (2) | US8206967B2 (zh) |
EP (1) | EP2179038B1 (zh) |
JP (1) | JP5661459B2 (zh) |
CN (1) | CN101688195B (zh) |
AU (1) | AU2008275828B2 (zh) |
BR (1) | BRPI0813743A2 (zh) |
CA (1) | CA2692656A1 (zh) |
WO (1) | WO2009008821A1 (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107267492A (zh) * | 2017-07-10 | 2017-10-20 | 中山大学 | 一种尖吻蝮蛇凝血酶重组蛋白的表达方法 |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0324044D0 (en) * | 2003-10-14 | 2003-11-19 | Astrazeneca Ab | Protein |
USRE46830E1 (en) | 2004-10-19 | 2018-05-08 | Polypeptide Laboratories Holding (Ppl) Ab | Method for solid phase peptide synthesis |
US7989193B2 (en) | 2005-04-13 | 2011-08-02 | Medimmune Limited | Compositions and methods for producing gamma-carboxylated proteins |
US8206967B2 (en) | 2007-07-06 | 2012-06-26 | Medimmune Limited | Method for production of recombinant human thrombin |
CN102690803B (zh) * | 2011-03-24 | 2015-04-29 | 苏州泽璟生物制药有限公司 | 一种重组人凝血酶在动物细胞内的高效表达和生产方法 |
EP2556842A1 (en) | 2011-08-11 | 2013-02-13 | Bioftalmik, S.L. | Composition in the form of film comprising fibrinogen and a fibrinogen activator and the applications thereof |
WO2020229521A1 (en) | 2019-05-14 | 2020-11-19 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods for inhibiting or reducing bacterial biofilms on a surface |
Citations (2)
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CN1551917A (zh) * | 2001-07-06 | 2004-12-01 | ���ŷ��˻�ѧ��Ѫ���Ʒ��о��� | 利用基因工程技术制备人凝血酶的方法 |
WO2005038019A1 (en) * | 2003-10-14 | 2005-04-28 | Astrazeneca Ab | Method for producing gamma-carboxylated proteins |
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DE3043857A1 (de) * | 1980-11-21 | 1982-07-08 | Behringwerke Ag, 3550 Marburg | Verfahren zur herstellung von blutgerinnungsfaktoren und danach hergestellte praeparation der faktoren ii und vii |
US4599308A (en) * | 1981-10-06 | 1986-07-08 | Hamer Dean H | Protein from SV40 recombinants |
DE3584341D1 (de) * | 1984-08-24 | 1991-11-14 | Upjohn Co | Rekombinante dna-verbindungen und expression von polypeptiden wie tpa. |
GR860984B (en) * | 1985-04-17 | 1986-08-18 | Zymogenetics Inc | Expression of factor vii and ix activities in mammalian cells |
AU8339187A (en) | 1986-11-17 | 1988-06-16 | New England Medical Center Hospitals, Inc., The | Enhancing gamma-carboxylation of recombinant vitamin k-dependent proteins |
US6224864B1 (en) * | 1986-12-03 | 2001-05-01 | Pharmacia & Upjohn Company | Antibiotic 10381A, and process for the preparation of anitbiotics 10381B |
JPH03501921A (ja) | 1987-05-18 | 1991-05-09 | インテグレイテッド・ジェネティクス・インク | 改良タンパク質分子、並びにその製造及び活性化方法 |
US5648254A (en) * | 1988-01-15 | 1997-07-15 | Zymogenetics, Inc. | Co-expression in eukaryotic cells |
CA1330302C (en) * | 1988-01-18 | 1994-06-21 | Miroslav Rybak | Concentrates of coagulation factors ii, vii, ix and x, method of their preparation and use |
AU8427091A (en) | 1990-07-23 | 1992-02-18 | Zymogenetics Inc. | Gamma-carboxylase and methods of use |
US5965789A (en) | 1991-01-11 | 1999-10-12 | American Red Cross | Engineering protein posttranslational modification by PACE/furin in transgenic non-human mammals |
US5268275A (en) | 1991-05-08 | 1993-12-07 | The University Of North Carolina At Chapel Hill | Vitamin K-dependent carboxylase |
US5219995A (en) | 1992-07-14 | 1993-06-15 | Alpha Therapeutic Corporation | Plasma fraction purification |
US6015686A (en) * | 1993-09-15 | 2000-01-18 | Chiron Viagene, Inc. | Eukaryotic layered vector initiation systems |
US5639661A (en) * | 1994-03-23 | 1997-06-17 | The University Of Iowa Research Foundation | Genes and proteins for treating cystic fibrosis |
DE4430204A1 (de) | 1994-08-26 | 1996-02-29 | Behringwerke Ag | Arzneimittel, welches als Gegenmittel für Blut-Antikoagulanzien geeignet ist und seine Verwendung |
DE59712322D1 (de) * | 1996-03-20 | 2005-06-30 | Baxter Ag | Pharmazeutisches Präparat zur Behandlung von Blutgerinnungsstörungen |
JP2002500011A (ja) | 1997-12-24 | 2002-01-08 | イミュネックス・コーポレーション | V201dna及びポリペプチド |
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WO2001007068A1 (en) | 1999-07-23 | 2001-02-01 | Case Western Reserve University | Novel methods and reagents for the treatment of osteoarthritis |
WO2001096587A2 (en) | 2000-06-13 | 2001-12-20 | The Children's Hospital Of Philadelphia | Methods for administering recombinant adeno-associated virus virions to humans previously exposed to adeno-associated virus |
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JP4813017B2 (ja) | 2001-07-10 | 2011-11-09 | 一般財団法人化学及血清療法研究所 | 薬剤学的に安定な止血用組成物 |
DK1842920T4 (da) | 2003-09-23 | 2017-08-21 | Univ North Carolina Chapel Hill | Celler der co-udtrykker K-vitaminreduktase og K-vitaminafhængigt protein, samt anvendelse deraf til at forbedre produktiviteten af det K-vitaminafhængige protein |
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-
2008
- 2008-07-03 US US12/167,614 patent/US8206967B2/en not_active Expired - Fee Related
- 2008-07-04 CN CN200880023664.2A patent/CN101688195B/zh not_active Expired - Fee Related
- 2008-07-04 AU AU2008275828A patent/AU2008275828B2/en not_active Ceased
- 2008-07-04 BR BRPI0813743-9A2A patent/BRPI0813743A2/pt not_active IP Right Cessation
- 2008-07-04 WO PCT/SE2008/050836 patent/WO2009008821A1/en active Application Filing
- 2008-07-04 CA CA 2692656 patent/CA2692656A1/en not_active Abandoned
- 2008-07-04 EP EP08779414.5A patent/EP2179038B1/en not_active Not-in-force
- 2008-07-04 JP JP2010514702A patent/JP5661459B2/ja not_active Expired - Fee Related
-
2012
- 2012-06-15 US US13/524,689 patent/US20120258090A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1551917A (zh) * | 2001-07-06 | 2004-12-01 | ���ŷ��˻�ѧ��Ѫ���Ʒ��о��� | 利用基因工程技术制备人凝血酶的方法 |
WO2005038019A1 (en) * | 2003-10-14 | 2005-04-28 | Astrazeneca Ab | Method for producing gamma-carboxylated proteins |
CN1867669A (zh) * | 2003-10-14 | 2006-11-22 | 阿斯特拉曾尼卡有限公司 | 生产伽马-羧化的蛋白的方法 |
Non-Patent Citations (1)
Title |
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YONEMURA,H.等: "Preparation of recombinant alpha-thrombin:high-level expression of recombinant human prethrombin-2 and its activation by recombinant ecarin", 《JOURNAL OF BIOCHEMISTRY》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107267492A (zh) * | 2017-07-10 | 2017-10-20 | 中山大学 | 一种尖吻蝮蛇凝血酶重组蛋白的表达方法 |
Also Published As
Publication number | Publication date |
---|---|
US20090047273A1 (en) | 2009-02-19 |
US20120258090A1 (en) | 2012-10-11 |
EP2179038B1 (en) | 2014-04-02 |
CN101688195B (zh) | 2014-12-03 |
BRPI0813743A2 (pt) | 2014-10-07 |
JP2010532661A (ja) | 2010-10-14 |
JP5661459B2 (ja) | 2015-01-28 |
AU2008275828B2 (en) | 2012-09-27 |
WO2009008821A1 (en) | 2009-01-15 |
CA2692656A1 (en) | 2009-01-15 |
US8206967B2 (en) | 2012-06-26 |
EP2179038A1 (en) | 2010-04-28 |
EP2179038A4 (en) | 2012-01-18 |
AU2008275828A1 (en) | 2009-01-15 |
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