CN101381684B - 一种暗褐网柄牛肝菌液体菌种的培养方法 - Google Patents
一种暗褐网柄牛肝菌液体菌种的培养方法 Download PDFInfo
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- CN101381684B CN101381684B CN2008102334694A CN200810233469A CN101381684B CN 101381684 B CN101381684 B CN 101381684B CN 2008102334694 A CN2008102334694 A CN 2008102334694A CN 200810233469 A CN200810233469 A CN 200810233469A CN 101381684 B CN101381684 B CN 101381684B
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- sucrose
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- 241001600007 Phlebopus portentosus Species 0.000 title claims abstract description 12
- 239000007788 liquid Substances 0.000 title claims description 38
- 230000001580 bacterial effect Effects 0.000 title claims description 12
- 238000012136 culture method Methods 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract description 24
- 239000001963 growth medium Substances 0.000 claims abstract description 21
- 239000002609 medium Substances 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 16
- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 16
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 16
- 229930006000 Sucrose Natural products 0.000 claims abstract description 16
- NGPGDYLVALNKEG-UHFFFAOYSA-N azanium;azane;2,3,4-trihydroxy-4-oxobutanoate Chemical compound [NH4+].[NH4+].[O-]C(=O)C(O)C(O)C([O-])=O NGPGDYLVALNKEG-UHFFFAOYSA-N 0.000 claims abstract description 16
- 235000015097 nutrients Nutrition 0.000 claims abstract description 16
- 239000005720 sucrose Substances 0.000 claims abstract description 16
- 238000002360 preparation method Methods 0.000 claims abstract description 12
- 229920001817 Agar Polymers 0.000 claims abstract description 11
- 239000008272 agar Substances 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 238000000855 fermentation Methods 0.000 claims abstract description 10
- 230000004151 fermentation Effects 0.000 claims abstract description 10
- 241000894006 Bacteria Species 0.000 claims description 12
- 239000007787 solid Substances 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 9
- 241000196324 Embryophyta Species 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 6
- 238000002386 leaching Methods 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- 230000004913 activation Effects 0.000 claims description 3
- 210000003811 finger Anatomy 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 235000013372 meat Nutrition 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 230000008520 organization Effects 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 210000003813 thumb Anatomy 0.000 claims description 3
- 238000012258 culturing Methods 0.000 abstract 3
- 239000012530 fluid Substances 0.000 abstract 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 abstract 2
- 239000007836 KH2PO4 Substances 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000009630 liquid culture Methods 0.000 abstract 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 abstract 1
- 235000019341 magnesium sulphate Nutrition 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241001489124 Boletus edulis Species 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
Description
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102334694A CN101381684B (zh) | 2008-10-22 | 2008-10-22 | 一种暗褐网柄牛肝菌液体菌种的培养方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102334694A CN101381684B (zh) | 2008-10-22 | 2008-10-22 | 一种暗褐网柄牛肝菌液体菌种的培养方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101381684A CN101381684A (zh) | 2009-03-11 |
| CN101381684B true CN101381684B (zh) | 2011-04-13 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008102334694A Active CN101381684B (zh) | 2008-10-22 | 2008-10-22 | 一种暗褐网柄牛肝菌液体菌种的培养方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101381684B (zh) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103724127B (zh) * | 2013-12-25 | 2015-09-23 | 魏晓辰 | 一种白蘑科真菌培养基及其制备方法和应用 |
| CN104370632B (zh) * | 2014-11-06 | 2017-06-30 | 丽水市农业科学研究院 | 一种提高牛肝菌菌丝体分离成活率的培养基 |
| CN106929432A (zh) * | 2017-03-09 | 2017-07-07 | 云南省热带作物科学研究所 | 一种暗褐网柄牛肝菌液体菌种深层发酵新方法 |
| CN108934785B (zh) * | 2018-06-28 | 2021-03-05 | 景洪宏臻农业科技有限公司 | 一种黑牛肝菌的液体菌种培养方法及栽培方法 |
| CN109055232A (zh) * | 2018-08-02 | 2018-12-21 | 周茂 | 一种暗褐网柄牛肝菌菌种制备方法 |
| CN109097288A (zh) * | 2018-08-02 | 2018-12-28 | 周茂 | 一种液体培养基、暗褐网柄牛肝菌工厂化液体菌种的制备方法及该液体培养基的用途 |
| CN111304095B (zh) * | 2020-03-10 | 2022-03-22 | 秦小波 | 牛肝菌菌丝可育培养基及其制备方法和应用纯化方法 |
| CN113412763B (zh) * | 2021-06-07 | 2021-11-23 | 云南省热带作物科学研究所 | 一株中华腐生牛肝菌菌株 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1220807A (en) * | 1967-05-17 | 1971-01-27 | Nat Res Dev | Production of fermentation and cultivation media |
| CN1273003C (zh) * | 2000-08-11 | 2006-09-06 | 株式会社世界 | 蘑菇液体菌种的制备方法及其接种装置 |
| CN101130755A (zh) * | 2007-07-19 | 2008-02-27 | 青岛大学 | 崂山食用牛肝菌液体菌种的培养方法 |
-
2008
- 2008-10-22 CN CN2008102334694A patent/CN101381684B/zh active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1220807A (en) * | 1967-05-17 | 1971-01-27 | Nat Res Dev | Production of fermentation and cultivation media |
| CN1273003C (zh) * | 2000-08-11 | 2006-09-06 | 株式会社世界 | 蘑菇液体菌种的制备方法及其接种装置 |
| CN101130755A (zh) * | 2007-07-19 | 2008-02-27 | 青岛大学 | 崂山食用牛肝菌液体菌种的培养方法 |
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| Publication number | Publication date |
|---|---|
| CN101381684A (zh) | 2009-03-11 |
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Denomination of invention: Culture method of phlebopus portentosus liquid bacterial Effective date of registration: 20200608 Granted publication date: 20110413 Pledgee: China Agricultural Development Bank Xishuangbanna Dai Autonomous Prefecture Branch Pledgor: JINGHONG HONGZHEN AGRICULTURAL SCIENCE AND TECHNOLOGY Co.,Ltd. Registration number: Y2020530000013 |
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Denomination of invention: A liquid culture method of Boletus obliquus Effective date of registration: 20210707 Granted publication date: 20110413 Pledgee: China Agricultural Development Bank Xishuangbanna Dai Autonomous Prefecture Branch Pledgor: JINGHONG HONGZHEN AGRICULTURAL SCIENCE AND TECHNOLOGY Co.,Ltd. Registration number: Y2021530000013 |
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Date of cancellation: 20220705 Granted publication date: 20110413 Pledgee: China Agricultural Development Bank Xishuangbanna Dai Autonomous Prefecture Branch Pledgor: JINGHONG HONGZHEN AGRICULTURAL SCIENCE AND TECHNOLOGY Co.,Ltd. Registration number: Y2021530000013 |
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Denomination of invention: A culture method of Boletus edulis liquid strain Effective date of registration: 20220714 Granted publication date: 20110413 Pledgee: China Agricultural Development Bank Xishuangbanna Dai Autonomous Prefecture Branch Pledgor: JINGHONG HONGZHEN AGRICULTURAL SCIENCE AND TECHNOLOGY Co.,Ltd. Registration number: Y2022530000019 |
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Date of cancellation: 20230711 Granted publication date: 20110413 Pledgee: China Agricultural Development Bank Xishuangbanna Dai Autonomous Prefecture Branch Pledgor: JINGHONG HONGZHEN AGRICULTURAL SCIENCE AND TECHNOLOGY Co.,Ltd. Registration number: Y2022530000019 |
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Denomination of invention: A Method for Cultivating Liquid Strain of Boletus edulis with Dark Brown Net Handle Effective date of registration: 20230811 Granted publication date: 20110413 Pledgee: China Agricultural Development Bank Xishuangbanna Dai Autonomous Prefecture Branch Pledgor: JINGHONG HONGZHEN AGRICULTURAL SCIENCE AND TECHNOLOGY Co.,Ltd. Registration number: Y2023530000055 |
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Denomination of invention: Cultivation method of liquid strain of Boletus edulis with dark brown web stem Granted publication date: 20110413 Pledgee: Yunnan Jinghong Rural Commercial Bank Co.,Ltd. Dongfeng Branch Pledgor: JINGHONG HONGZHEN AGRICULTURAL SCIENCE AND TECHNOLOGY Co.,Ltd. Registration number: Y2024980041346 |