CN101349644B - 一种白细胞分类试剂和其使用方法 - Google Patents

一种白细胞分类试剂和其使用方法 Download PDF

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Publication number
CN101349644B
CN101349644B CN200710139172.7A CN200710139172A CN101349644B CN 101349644 B CN101349644 B CN 101349644B CN 200710139172 A CN200710139172 A CN 200710139172A CN 101349644 B CN101349644 B CN 101349644B
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fluorescent dye
leucocyte
reagent according
leucocyte classification
classification reagent
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Chinese (zh)
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CN101349644A (zh
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徐兵
张宝华
匡玉吉
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Shenzhen Mindray Bio Medical Electronics Co Ltd
Shenzhen Mindray Scientific Co Ltd
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Priority to US11/967,897 priority patent/US7960099B2/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
CN200710139172.7A 2007-07-20 2007-07-20 一种白细胞分类试剂和其使用方法 Active CN101349644B (zh)

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CN200710139172.7A CN101349644B (zh) 2007-07-20 2007-07-20 一种白细胞分类试剂和其使用方法
US11/967,897 US7960099B2 (en) 2007-07-20 2007-12-31 White blood cell differentiation reagent containing an asymmetric cyanine fluorescent dye and method of use thereof

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CN200710139172.7A CN101349644B (zh) 2007-07-20 2007-07-20 一种白细胞分类试剂和其使用方法

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CN101726579B (zh) * 2008-10-17 2014-06-18 深圳迈瑞生物医疗电子股份有限公司 血液检测试剂和方法
CN101750274B (zh) * 2008-12-17 2014-06-25 深圳迈瑞生物医疗电子股份有限公司 白细胞分类计数试剂、试剂盒以及白细胞分类计数的方法
CN101988082B (zh) * 2009-07-31 2015-04-08 深圳迈瑞生物医疗电子股份有限公司 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法
CN102226804B (zh) * 2011-03-28 2013-07-03 中国人民解放军总医院 一种用于血液白细胞五分类计数的溶血剂及其用途
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CN104749144A (zh) * 2013-12-31 2015-07-01 深圳迈瑞生物医疗电子股份有限公司 血细胞检测试剂及血细胞处理方法和识别方法
CN104190314B (zh) * 2014-08-15 2016-06-08 中国地质大学（北京） 脂肪胺聚氧乙烯醚二乙基双磺酸盐表面活性剂及其制备方法
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WO2016106688A1 (zh) * 2014-12-31 2016-07-07 深圳迈瑞生物医疗电子股份有限公司 一种有核红细胞报警方法、装置及流式细胞分析仪
CN105986003B (zh) * 2015-02-12 2020-11-13 深圳迈瑞生物医疗电子股份有限公司 一种白细胞计数方法、装置及细胞分析仪
CN104698157B (zh) * 2015-02-13 2017-05-17 中山市创艺生化工程有限公司 血细胞分析仪用试剂
CN105004641A (zh) * 2015-08-27 2015-10-28 苏州柯尔医疗器械有限公司 一种血液分析仪及分析方法
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CN114127535A (zh) * 2019-06-19 2022-03-01 深圳迈瑞动物医疗科技有限公司 一种细胞分析仪、基于阻抗法对白细胞进行分类的方法及计算机可读存储介质
WO2021087730A1 (zh) * 2019-11-05 2021-05-14 深圳迈瑞生物医疗电子股份有限公司 白细胞分类试剂及其使用方法
CN111896757B (zh) * 2020-08-04 2023-08-29 武汉生之源生物科技股份有限公司 一种改善全血样本测值的d-二聚体测定试剂盒、制备方法及应用
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