CN101288376A - Technique for reducing the maturation phase of Nothapodytes nimmoniana (Graham) Mablerley and increasing camptothecine content - Google Patents
Technique for reducing the maturation phase of Nothapodytes nimmoniana (Graham) Mablerley and increasing camptothecine content Download PDFInfo
- Publication number
- CN101288376A CN101288376A CNA2008100287415A CN200810028741A CN101288376A CN 101288376 A CN101288376 A CN 101288376A CN A2008100287415 A CNA2008100287415 A CN A2008100287415A CN 200810028741 A CN200810028741 A CN 200810028741A CN 101288376 A CN101288376 A CN 101288376A
- Authority
- CN
- China
- Prior art keywords
- plant
- nothapodytes
- fertilizer
- centimetre
- content
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a technical method for shortening the maturation period of nothapodytes and increasing the camptothecin content thereof. The method is as follows: selecting nothapodytes; establishing seedling cultivation ground; digging planting holes in a planting base, and applying sufficient base fertilizer in the holes, wherein the content of fertilizer is 50% of phosphorus fertilizer, 30% of nitrogenous fertilizer and 20% of kalium fertilizer, and 10 centimeter of the depth of backfilled soil; planting the treated seedlings in the seedling cultivation ground into the holes, watering once, keeping the temperature at 26 DEG C and relative humidity at 65%; kneading the tree skin on the connection of the wood section and the semi-wood section when the survival plants grow by 20 centimeters, kneading once when the plants grow by every 20 centimeters. The method of the invention has the advantages that: compared with the wild nothapodytes, the growth period of planted nothapodytes is reduced by 6-12 years; the camptothecin content thereof is increased from 0.00001 to 0.000235, thus increasing benefit and labor efficiency, dramatically saving land resource and guaranteeing the market supply of camptothecin.
Description
Technical field
The present invention relates to a kind of technical method that can make plant speed give birth to and again pharmaceutical ingredient content is wherein increased, particularly a kind of technical method that shortens the form nothapodytes maturing stage, improves its camptothecine content.
Background technology
At present, the camptothecine of anti-tumor active ingredient, the channel of its collection is mainly derived from form nothapodytes.Because the growth cycle of wild form nothapodytes is very long, need growth 8-15, the height of tree just can reach the 200-300 centimetre, and content of camptothecine has only 1/10000ths in it, so slowly long growth cycle and low content like this, cause the investment repayment cycle long, efficiency is low, has improved the cost of raw material greatly, has wasted land resources greatly.
Summary of the invention
The objective of the invention is to remedy above-mentioned all deficiencies, a kind of technical method that shortens the form nothapodytes maturing stage, improves its camptothecine content is provided.
Base reason of the present invention is: according to the ecological basic theories of natural resources of Chinese medicinal materials, by improving biological growing environment, reach biological mushroom purpose, for the form nothapodytes kind, under the situation of fully realizing its basic life habit, make full use of envirment factor such as temperature, water, fertilizer etc., the growing environment of form nothapodytes plant is implemented regulation and control, thereby reach the purpose that form nothapodytes speed is given birth to.The characteristics of form nothapodytes are extremely to be afraid of to tremble with fear, and the temperature of environment just is difficult to existence, so at nursery, the environmental temperature that it is peripheral is set between 20 ℃-26 ℃ below 10 ℃ the time; Aspect fertilising, to grow fast in order to make form nothapodytes, the proportioning of the phosphate fertilizer in the used fertilizer, nitrogenous fertilizer, potash fertilizer is 5: 3: 2; Relative moisture remains between the 50%-60%.
In order to improve the content of the camptothecine in the form nothapodytes, adopted the principle of the adverse circumstance effect of biological growth in the biology, the camptothecine in the form nothapodytes concerning form nothapodytes, has the effect of resisting external infringement.Therefore; in the home that the nascent metabolism growth of form nothapodytes is grown; implement improper environment conditioning; form nothapodytes is adopted the environment-stress means of slight damage; impel form nothapodytes to improve the defense mechanism of self; the camptothecine that irritates in the form nothapodytes forms fast, accumulates and discharges, thereby reaches the purpose of the content that improves the camptothecine in the form nothapodytes.In addition, implement improper environment conditioning, the environment-stress means to form nothapodytes employing slight damage can also play the effect of the growth year that shortens trees.
The step of its technical method is as follows:
1, selects Icacinaceae dungarunga---form nothapodytes;
2, set up one and have the nursery of transplanting facility, set by 600-700 strain form nothapodytes seedling for every mu;
3, build the canopy of transplanting seedlings of sealing at nursery, and the control temperature and humidity;
4, in the canopy of transplanting seedlings, earlier transplanted seedling is completely cured, remove the old blade of plant, handle well, not to the watering of transplanting seedlings, guarantee the vitality of transplanted seedling;
5, planting base that planning and designing are good at first requires to dig to plant plant hole, uses sufficient base manure in the cave, fertilizer is phosphate fertilizer 50%, nitrogenous fertilizer 30%, potash fertilizer 20%, the thickness of backfill soil is the 8-12 centimetre, and matrix is loose, permeable, ventilative, has certain preserve moisture and fertility ability;
6, the processing seedling that the old blade of plant was completely cured, was removed to doing in the nursery, be transported to the planting base, the processing seedling that selection does not occur growing and stops phenomenon being planted it in plant pit, and base manure can not directly contact with shoot root, compacting after the backfill soil, pour water 1 time, temperature is between 22 ℃-29 ℃ in summer, and relative moisture is when 60%-70%, plant, sapling is taken root fast in initial planting;
7, after the plant that survives grows tall the 18-22 centimetre, it is adopted the environment-stress means of slight damage;
8, through behind the 2-3, this tree just is tending towards becoming a useful person, and the height of tree can reach 150 centimeters-200 centimeters, and the tree bar can reach the 2-3 centimetre.
Superiority of the present invention is: after adopting above-mentioned method, shortened the time limit that form nothapodytes is become a useful person, the time limit of becoming a useful person is compared with wild form nothapodytes and has been shifted to an earlier date 6-12, improved the content of the camptothecine in it, content has brought up to 2/10000ths. 35 by original 1/10000ths, thereby has improved investment return and efficiency greatly, has saved land resources greatly, fully guarantee the market supply of camptothecine, thereby safeguarded the physical and mental health of extensive patients conscientiously.
Specific embodiments
1, selects Icacinaceae dungarunga---form nothapodytes;
2, set up one and have the nursery of transplanting facility, prepare 660 strain form nothapodytes seedlings for every mu;
3, build the canopy of transplanting seedlings of sealing at nursery, the temperature of the canopy of transplanting seedlings remains between 22-26 ℃, and relative moisture remains on 50%-60%;
4, in the canopy of transplanting seedlings of nursery, earlier transplanted seedling is completely cured, remove the old blade of plant, not to the watering of transplanting seedlings;
5, planting base that planning and designing are good at first requires to dig to plant plant hole, uses sufficient base manure in the cave, and fertilizer is phosphate fertilizer 50%, nitrogenous fertilizer 30%, potash fertilizer 20%, and the thickness of backfill soil is 10 centimeters, and matrix is loose, permeable, ventilative, has certain preserve moisture and fertility ability;
6, the processing seedling that the old blade of plant was completely cured, was removed to doing in the nursery, be transported to the planting base, the processing seedling that selection does not occur growing and stops phenomenon, it is planted in plant pit, and base manure can not directly contact with shoot root, compacting after the backfill soil, pour water 1 time, temperature remains on about 25 ℃, and relative moisture remains on about 65%, and sapling is taken root fast in initial planting;
7, adopt the environment-stress means of slight damage to be: when the plant that survives grows tall between the 18-22 centimetre, the bark that rubs press...withes one's finger in wooden section of process of growth of every strain trees and half wooden section junction, morning or massage in evening are once, make and cause bark and sustain a slight injury, injured area is about 50% of a plant, whenever upwards long when the 20-30 centimetre, just use the same method and massage; Each technical application method is after 6 days, and dispensing organic nitrogen-containing fertilizer once.
8, through after 3 years, this tree just is tending towards becoming a useful person, and tree reaches as high as 200 centimeters, and the tree bar can reach 3 centimeters.
Claims (3)
1. technical method that shortens the form nothapodytes maturing stage, improves its camptothecine content, it is characterized in that: concrete grammar is as follows:
A. select the Icacinaceae dungarunga---the form nothapodytes kind;
B. set up one and have the nursery of transplanting facility, prepare 600-700 strain form nothapodytes seedling for every mu;
C. build the canopy of transplanting seedlings of sealing at nursery, and the control temperature and humidity;
D. in the canopy of transplanting seedlings of nursery, earlier transplanted seedling is completely cured, removes the old blade of plant, put back to original position then, and control the temperature and the relative moisture of the canopy of transplanting seedlings;
E. dig in the planting base and plant plant hole, use sufficient base manure in the cave, and control the proportioning of phosphate fertilizer, nitrogenous fertilizer, potash fertilizer, native in the backfill then, the thickness of backfill soil is the 8-12 centimetre;
F. the processing seedling that the old blade of plant was completely cured, was removed to doing in the nursery, be transported to the planting base, it is planted in plant pit, and base manure can not directly contact with shoot root, compacting after the backfill soil, pour water 1 time, in summer, temperature is between 22 ℃-29 ℃, and relative moisture is when 60%-70%, plant, sapling is taken root fast in initial planting;
G. after the plant that survives grows tall the 18-22 centimetre, it is adopted the environment-stress means of slight damage;
H. after passing through 2-3, this tree just is tending towards becoming a useful person, and the height of tree can reach 150 centimeters-200 centimeters, and the tree bar can reach the 2-3 centimetre.
2, a kind of technical method that shortens the form nothapodytes maturing stage, improves its camptothecine content according to claim 1, it is characterized in that: the temperature of the canopy of transplanting seedlings is controlled between 20 ℃-26 ℃, and relative moisture remains between the 50%-60%.
3, a kind of shortening form nothapodytes maturing stage according to claim 1, improve the technical method of its camptothecine content, it is characterized in that: adopt the environment-stress means of slight damage to be: when the plant that survives grows tall between the 18-22 centimetre, the bark that rubs press...withes one's finger in wooden section of process of growth of every strain trees and half wooden section junction, morning or massage in evening are once, make and cause bark and sustain a slight injury, injured area is about 50% of a plant, whenever upwards long when the 20-30 centimetre, just use the same method and massage, each technical application method is after 6 days, and dispensing organic nitrogen-containing fertilizer once.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100287415A CN101288376A (en) | 2008-06-12 | 2008-06-12 | Technique for reducing the maturation phase of Nothapodytes nimmoniana (Graham) Mablerley and increasing camptothecine content |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100287415A CN101288376A (en) | 2008-06-12 | 2008-06-12 | Technique for reducing the maturation phase of Nothapodytes nimmoniana (Graham) Mablerley and increasing camptothecine content |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101288376A true CN101288376A (en) | 2008-10-22 |
Family
ID=40032964
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008100287415A Pending CN101288376A (en) | 2008-06-12 | 2008-06-12 | Technique for reducing the maturation phase of Nothapodytes nimmoniana (Graham) Mablerley and increasing camptothecine content |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101288376A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102948370A (en) * | 2012-12-13 | 2013-03-06 | 向华 | Rapid propagation method of nothapodytes pittosporoides |
| CN103181272A (en) * | 2011-12-28 | 2013-07-03 | 广东神农衍生物科技有限公司 | Artificial planting method for medical raw materials of nothapodytes nimmoniana |
| CN104686147A (en) * | 2015-02-16 | 2015-06-10 | 唐忠海 | Large-scale mappia pittosporoides seedling and cultivation method |
-
2008
- 2008-06-12 CN CNA2008100287415A patent/CN101288376A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103181272A (en) * | 2011-12-28 | 2013-07-03 | 广东神农衍生物科技有限公司 | Artificial planting method for medical raw materials of nothapodytes nimmoniana |
| CN102948370A (en) * | 2012-12-13 | 2013-03-06 | 向华 | Rapid propagation method of nothapodytes pittosporoides |
| CN104686147A (en) * | 2015-02-16 | 2015-06-10 | 唐忠海 | Large-scale mappia pittosporoides seedling and cultivation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103238438B (en) | Three-dimensional interplanting method of Chinese yew, conifer and fern | |
| CN101611688A (en) | The propagation method of using oil-tea young anvil to graft containerized seedlings | |
| CN104620813B (en) | A kind of normalized planting method of medicinal plant blackberry lily | |
| CN102893788A (en) | Method for cuttage of taxus chinensis | |
| CN104255261A (en) | Good-quality and high-yield cultivation method of radix puerariae | |
| CN105409531A (en) | Disease and insect pest occurrence reducing lotus root planting method for lotus root ponds | |
| CN107258448A (en) | A kind of high-yield planting method of jujube tree | |
| CN105052654A (en) | Dragon fruit cultivation method | |
| CN106358827A (en) | Cutting seedling transplanting method of chrysanthemum | |
| CN104770173A (en) | High-efficiency seedling cultivation method for promoting premature tillering of sugarcane tissue cultured seedlings | |
| CN106034898A (en) | Planting method of bergamot | |
| CN104365350A (en) | High-quality and high-efficiency cultivation technology for lettuces in open field | |
| CN104365347A (en) | Celery open-field high-quality and high-efficiency cultivation technology | |
| CN105660080A (en) | Large-scale mint cultivation method | |
| CN104380985A (en) | Open-field, high-quality and efficient cultivation technology for lettuce | |
| CN107278778A (en) | A kind of implantation methods of high yield fruit mulberry | |
| CN101288376A (en) | Technique for reducing the maturation phase of Nothapodytes nimmoniana (Graham) Mablerley and increasing camptothecine content | |
| CN104429430A (en) | Method for planting taxuschinensis tea in garden | |
| CN109618696A (en) | A kind of method that fast breeding is transplanted in 3 repetitions of sugarcane health seedling original seedling | |
| CN104145761A (en) | Method for lifting and transplanting fig seedlings | |
| CN103766116A (en) | Cultivation method for lavender | |
| CN107155793A (en) | Interplant the implantation methods of wax gourd in a kind of peach garden | |
| CN103918502B (en) | Multi-functional blade of grass potato culture basin | |
| CN101416586A (en) | The cottage method of a kind of Chinese rose | |
| CN104429489A (en) | Wild celery open-field high-quality and high-efficiency cultivation technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20081022 |