CN100471522C - 聚核苷酸组合物及其制备方法与用途 - Google Patents
聚核苷酸组合物及其制备方法与用途 Download PDFInfo
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- CN100471522C CN100471522C CNB998038741A CN99803874A CN100471522C CN 100471522 C CN100471522 C CN 100471522C CN B998038741 A CNB998038741 A CN B998038741A CN 99803874 A CN99803874 A CN 99803874A CN 100471522 C CN100471522 C CN 100471522C
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- polynucleotide
- plasmid dna
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- cryoprotective agent
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- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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Abstract
Description
成分 | 冷冻干燥前的聚核苷酸溶液 | 液体对照组合物 |
质粒DNA | 0.2%w/v | 0.2%w/v |
蔗糖 | 2.0%w/v | --- |
磷酸盐缓冲液(5mM) | 0.045%w/v磷酸二氢钠,0.047%w/v磷酸氢钠 | --- |
注射用水(qs) | 100ml | 100ml |
布比卡因(促进剂) | --- | 0.25%w/v |
EDTA | --- | 0.01%w/v |
柠檬酸盐缓冲液 | --- | 30mM |
pH | 6.7 | 6.4 |
冷冻干燥周期 | 温度(℃) | 真空(mTorr) | 时间(分钟) | 渐变/保持 |
冷冻步骤 | -40 | 200 | 30 | 保持 |
首次干燥(2) | 0 | 100 | 30 | 渐变 |
0 | 100 | 720 | 保持 | |
二次干燥(4) | 30 | 75 | 20 | 渐变 |
30 | 75 | 180 | 保持 | |
25 | 50 | 5 | 渐变 | |
25 | 50 | 120 | 保持 |
成分 | 冷冻干燥前的聚核苷酸溶液 | 液体对照组合物 |
质粒DNA | 0.2%w/v | 0.2%w/v |
海藻糖 | 2.0%w/v | --- |
磷酸盐缓冲液(5mM) | 0.045%w/v磷酸二氢钠,0.047%w/v磷酸氢钠 | --- |
注射用水(qs) | 100ml | 100ml |
布比卡因(促进剂) | --- | 0.25%w/v |
EDTA | --- | 0.01%w/v |
柠檬酸盐缓冲液 | --- | 30mM |
pH | 6.7 | 6.4 |
组合物编号# | DNA(mg/ml) | 冷冻保护剂(%w/w) | 含水量(%)冷冻后 | kobs(95%CI)×10<sup>-4</sup>天<sup>-1</sup>冷冻干燥后 | R2冷冻干燥后 |
96F-0254 | 1 | --- | --- | 139.8±117 | 0.93 |
1S1L | 1 | 蔗糖1% | 2 | 12.3±20.39 | 0.77 |
1S2L | 1 | 蔗糖2% | 2 | 4.0±6.4 | 0.77 |
1T1L | 1 | 海藻糖1% | 2 | 66.5±46.2 | 0.95 |
1T2L | 1 | 海藻糖2% | 2 | 94.1±28.9 | 0.99 |
2S1L | 2 | 蔗糖1% | 2 | 11.2±8.6 | 0.94 |
2S2L | 2 | 蔗糖2% | 2 | 10.2±15.5 | 0.80 |
2T1L | 2 | 海藻糖1% | 2 | 40.5±28.2 | 0.95 |
2T2L | 2 | 海藻糖2% | 2 | 55.0±7.6 | 1.00 |
1S1H | 1 | 蔗糖1% | 4 | 18.9±43.1 | 0.64 |
1S2H | 1 | 蔗糖2% | 4 | 3.1±10 | 0.47 |
1T1H | 1 | 海藻糖1% | 4 | 48.5±24.9 | 0.97 |
1T2H | 1 | 海藻糖2% | 4 | 41.9±41 | 0.91 |
2S1H | 2 | 蔗糖1% | 4 | 6.1±20 | 0.47 |
2S2H | 2 | 蔗糖2% | 4 | 21.6±19.2 | 0.92 |
2T1H | 2 | 海藻糖1% | 4 | 26.1±20.1 | 0.94 |
2T2H | 2 | 海藻糖2% | 4 | 32.5±1.4 | 1.00 |
Claims (19)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US7808098P | 1998-03-13 | 1998-03-13 | |
US60/078,080 | 1998-03-13 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1294520A CN1294520A (zh) | 2001-05-09 |
CN100471522C true CN100471522C (zh) | 2009-03-25 |
Family
ID=22141799
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB998038741A Expired - Fee Related CN100471522C (zh) | 1998-03-13 | 1999-03-12 | 聚核苷酸组合物及其制备方法与用途 |
Country Status (14)
Country | Link |
---|---|
US (1) | US20080081366A1 (zh) |
EP (1) | EP1061955B1 (zh) |
JP (1) | JP2002506048A (zh) |
KR (1) | KR100625385B1 (zh) |
CN (1) | CN100471522C (zh) |
AT (1) | ATE294594T1 (zh) |
AU (1) | AU765177B2 (zh) |
BR (1) | BR9908754A (zh) |
CA (1) | CA2322232A1 (zh) |
DE (1) | DE69925113T2 (zh) |
ES (1) | ES2239440T3 (zh) |
IL (1) | IL138306A0 (zh) |
PT (1) | PT1061955E (zh) |
WO (1) | WO1999045966A1 (zh) |
Families Citing this family (27)
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WO1999061063A1 (fr) * | 1998-05-22 | 1999-12-02 | Sumitomo Pharmaceuticals Company, Limited | Preparations geniques stables |
DE69931166T2 (de) * | 1998-08-14 | 2007-02-15 | Valentis Inc., Burlingame | Co-lyophilisierter komplex umfassend einen nukleinsäurevektor und ein formulierungsagens |
DE60026571T2 (de) * | 1999-11-19 | 2006-12-14 | MGI Pharma Biologics, Inc., Lexington | Durchflussverfahren zur herstellung von mikropartikeln |
JP2001316297A (ja) * | 2000-02-23 | 2001-11-13 | Kaken Pharmaceut Co Ltd | 遺伝子包埋リポソーム製剤及びその製法 |
PT1456377T (pt) | 2001-12-20 | 2019-09-10 | Merck Sharp & Dohme | Composições de syn3 e processos |
BR0308441A (pt) | 2002-03-15 | 2005-01-18 | Wyeth Corp | Proteìna variante de p4 de haemophilus influenzae não tipificável, composição imunogênica, molécula de nucleotìdeo isolada, célula hospedeira, e, métodos para induzir uma resposta imune em um ser humano contra h. influenzae não tipificável, e para produzir uma proteìna variante de p4 |
AU2003218168A1 (en) * | 2002-03-15 | 2003-09-29 | University Of Southern California | Ophthalmic solutions for delivery of expression vectors |
EP1578193A4 (en) * | 2002-12-23 | 2011-06-15 | Vical Inc | FREEZING PROCESS FOR NUCLEIC ACID / BLOCK COPOLYMER / CATION SIDE COMPLEXES |
WO2004060363A1 (en) | 2002-12-23 | 2004-07-22 | Vical Incorporated | Method for producing sterile polynucleotide based medicaments |
TW200613554A (en) | 2004-06-17 | 2006-05-01 | Wyeth Corp | Plasmid having three complete transcriptional units and immunogenic compositions for inducing an immune response to HIV |
EP1954252B1 (en) * | 2005-12-02 | 2016-02-03 | GlaxoSmithKline Biologicals SA | Nanoparticles for use in immunogenic compositions |
KR100777249B1 (ko) | 2006-02-14 | 2007-11-28 | (주)바이오니아 | 건조 올리고뉴클레오티드 조성물 및 이의 제조 방법 |
DE102006038240A1 (de) * | 2006-08-07 | 2008-02-14 | Biotronik Vi Patent Ag | Verfahren zur Herstellung eines Komposits aus Oligo- oder Polynucleotiden und hydrophoben biodegradierbaren Polymeren sowie nach dem Verfahren erhaltenes Komposit |
GB0701253D0 (en) | 2007-01-23 | 2007-02-28 | Diagnostics For The Real World | Nucleic acid amplification and testing |
EP1970441A1 (en) * | 2007-03-06 | 2008-09-17 | BioAlliance Pharma | Plasmid containing a sequence encoding a disintegrin domain of metargidin (RDD) |
ES2556711T3 (es) | 2008-04-09 | 2016-01-19 | Viromed Co., Ltd. | Formulaciones liofilizadas de ADN para el aumento de la expresión del ADN plasmídico |
AU2012267578B2 (en) | 2011-06-08 | 2017-04-20 | Translate Bio, Inc. | Cleavable lipids |
CN104854128A (zh) | 2012-07-19 | 2015-08-19 | 硕腾有限责任公司 | 牛流感病毒组合物 |
RU2542385C2 (ru) | 2012-08-31 | 2015-02-20 | Общество с ограниченной ответственностью "НекстГен" | Способ получения фармацевтической композиции для индукции развития кровеносных сосудов в тканях, фармацевтическая композиция, полученная этим способом, и способ лечения ишемии тканей и/или органов человека |
ES2773305T3 (es) | 2013-10-22 | 2020-07-10 | Helixmith Co Ltd | Composición para la prevención o tratamiento de la esclerosis lateral amiotrófica que utiliza dos o más isoformas del factor de crecimiento de hepatocitos |
JP6462723B2 (ja) * | 2014-12-29 | 2019-01-30 | 株式会社ボナック | 核酸分子を安定に含有する組成物 |
RU2612497C2 (ru) | 2015-05-26 | 2017-03-09 | Общество с ограниченной ответственностью "НекстГен" | Оптимизированная нуклеотидная последовательность и фармацевтическая композиция на ее основе с пролонгированной экспрессией трансгена vegf |
MX2018001841A (es) | 2015-08-14 | 2018-08-01 | Zoetis Services Llc | Composiciones de mycoplasma bovis. |
CA3042689A1 (en) | 2016-11-04 | 2018-07-12 | Baxalta Incorporated | Adeno-associated virus formulations |
JP7212230B2 (ja) | 2018-07-19 | 2023-01-25 | ヘリックスミス カンパニー, リミテッド | ネイキッドdna遺伝子療法のための凍結乾燥した薬剤学的組成物 |
CN110596227B (zh) * | 2019-08-08 | 2021-11-23 | 河北省食品检验研究院(国家果类及农副加工产品质量监督检验中心、河北省食品安全实验室) | 飞行时间质谱即用型斯坦利沙门氏菌定性标准样品的制备方法 |
KR102460006B1 (ko) * | 2021-11-22 | 2022-10-27 | 주식회사 리엔젠 | 폴리뉴클레오타이드 및 히알루론산 포함하는 피부 필러 조성물의 동결건조 제조방법 및 이에 따라 제조된 폴리뉴클레오타이드 및 히알루론산 포함하는 피부 필러 조성물 |
Family Cites Families (8)
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AU2309692A (en) * | 1991-07-03 | 1993-02-11 | Cryolife, Inc. | Method for stabilization of biomaterials |
US5709076A (en) * | 1992-09-14 | 1998-01-20 | Lawlor; Shawn P. | Method and apparatus for power generation using rotating ramjet which compresses inlet air and expands exhaust gas against stationary peripheral wall |
JPH08511956A (ja) * | 1994-04-07 | 1996-12-17 | アクゾ・ノベル・エヌ・ベー | Rnaを含む凍結乾燥組成物 |
WO1996027393A1 (en) * | 1995-03-07 | 1996-09-12 | University Of Pittsburgh | A dry powder formulation for gene therapy |
US5811406A (en) * | 1995-06-07 | 1998-09-22 | Regents Of The University Of California | Dry powder formulations of polynucleotide complexes |
CA2252565C (en) * | 1996-04-26 | 2010-11-23 | David B. Volkin | Dna vaccine formulations |
ZA973642B (en) * | 1996-04-26 | 1997-11-25 | Merck & Co Inc | DNA vaccine formulations. |
DE19716154A1 (de) * | 1997-04-18 | 1998-10-22 | Boehringer Mannheim Gmbh | Stabile pharmazeutische Darreichungsform für Peptide, Proteine und Nukleinsäuren |
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1999
- 1999-03-12 WO PCT/US1999/005547 patent/WO1999045966A1/en active IP Right Grant
- 1999-03-12 KR KR1020007009929A patent/KR100625385B1/ko not_active IP Right Cessation
- 1999-03-12 CN CNB998038741A patent/CN100471522C/zh not_active Expired - Fee Related
- 1999-03-12 EP EP99912502A patent/EP1061955B1/en not_active Expired - Lifetime
- 1999-03-12 JP JP2000535379A patent/JP2002506048A/ja active Pending
- 1999-03-12 IL IL13830699A patent/IL138306A0/xx not_active IP Right Cessation
- 1999-03-12 CA CA002322232A patent/CA2322232A1/en not_active Abandoned
- 1999-03-12 ES ES99912502T patent/ES2239440T3/es not_active Expired - Lifetime
- 1999-03-12 BR BR9908754-5A patent/BR9908754A/pt not_active Application Discontinuation
- 1999-03-12 DE DE69925113T patent/DE69925113T2/de not_active Expired - Fee Related
- 1999-03-12 PT PT99912502T patent/PT1061955E/pt unknown
- 1999-03-12 AT AT99912502T patent/ATE294594T1/de not_active IP Right Cessation
- 1999-03-12 AU AU30868/99A patent/AU765177B2/en not_active Ceased
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Publication number | Publication date |
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US20080081366A1 (en) | 2008-04-03 |
EP1061955A1 (en) | 2000-12-27 |
CA2322232A1 (en) | 1999-09-16 |
DE69925113D1 (de) | 2005-06-09 |
CN1294520A (zh) | 2001-05-09 |
DE69925113T2 (de) | 2006-01-19 |
ATE294594T1 (de) | 2005-05-15 |
PT1061955E (pt) | 2005-08-31 |
BR9908754A (pt) | 2000-11-28 |
WO1999045966A1 (en) | 1999-09-16 |
KR100625385B1 (ko) | 2006-09-18 |
IL138306A0 (en) | 2001-10-31 |
ES2239440T3 (es) | 2005-09-16 |
JP2002506048A (ja) | 2002-02-26 |
AU3086899A (en) | 1999-09-27 |
AU765177B2 (en) | 2003-09-11 |
KR20010074441A (ko) | 2001-08-04 |
EP1061955B1 (en) | 2005-05-04 |
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