CN100372451C - Propagation method of female parent for oriental lily planting - Google Patents
Propagation method of female parent for oriental lily planting Download PDFInfo
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- CN100372451C CN100372451C CNB2005100109359A CN200510010935A CN100372451C CN 100372451 C CN100372451 C CN 100372451C CN B2005100109359 A CNB2005100109359 A CN B2005100109359A CN 200510010935 A CN200510010935 A CN 200510010935A CN 100372451 C CN100372451 C CN 100372451C
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Abstract
The invention discloses a method for breeding female parents for planting oriental lilies, which comprises the steps of selecting healthy bulbs with the specification of 14-22 cm in 8-10 months, dividing 5 layers of scales on the periphery, fully cleaning and disinfecting, embedding the scales into a matrix, planting the embedded scales in a seedbed after heat treatment and cold treatment respectively, carrying out seedbed management according to a conventional process, and growing overground stem plants after 30-40 days. The method overcomes the defects of the prior art, can effectively control the development type of the oriental lily bulblets, greatly improves the proportion of overground stem plants, ensures that the cultivated bulblets can generate a large number of large-sized planting female parents in the first year of planting, shortens the planting period, reduces the production cost, and has good market application prospect.
Description
Technical field
The invention belongs to field of plant growing technology, be specifically related to a kind of propagation method of female parent of oriental lily.
Background technology
Oriental hybrid lily (Lilium spp.cv.oriental hybrids) is a hybridization strain in the lilium, is one of flowering bulb most popular on the international market.By international market transaction convention, with perpendicular to the maximum bulb circumference of bulb central authorities main bud as the standard of dividing oriental hybrid lily bulb specification, that wherein specification 12cm is following is plantation maternal (planting stocking), that 12cm is above is split (flowering bulbs), and split is mainly used in the production cut-flower.
Because the great demand in market, China is maternal from external a large amount of import oriental hybrid lily splits and plantation every year, is example with 2004 only, about 6,000 ten thousand of import split, maternal about 6,000,000 of plantation.Not only expend a large amount of foreign exchanges, and increased the operating cost and the risk of enterprise.Therefore, since the mid-90, how China units concerned utilizes the scale propagation technique to produce female parent of oriental lily if just beginning one's study from eighties of last century.
Find that after deliberation when utilizing the numerous plantation of oriental hybrid lily scale expansion maternal, the clove that scale is grown has two kinds of plant to grow types, a kind of is the basal leaf sheet, and another kind of is the plant with terrestrial stem.This have terrestrial stem plant, have advantages such as blade quantity is many, big, the underground bulb growth of photosynthesis area is fast.But existing propagation technique still can not be controlled the plant development type of clove effectively; plant and grew that the terrestrial stem plant that only accounts for below 1% of plant sum, the maternal ratio of the above plantation of results specification 6cm only accounts for 10% of whole harvest yields in 1 year; even lower, can not satisfy the needs of large-scale planting far away.
Summary of the invention
Technical problem to be solved by this invention is at the deficiencies in the prior art, a kind of artificial fecundation method of female parent of oriental lily is provided, pass through the artificial induction, the clove that makes scale grow just can develop into the plant with terrestrial stem in a large number at 1 year that plants, significantly improve the maternal output of the above plantation of specification 6cm, and then shorten crop cycle, reduce production costs.
Purpose of the present invention is achieved by the following technical programs.
Except as otherwise noted, the percentage of the present invention's employing is percetage by weight.
The invention provides a kind of propagation method of female parent of oriental lily, this method comprises the steps:
1, matrix is prepared
Select fresh peat soil as matrix, the sterilization back is standby;
2, the bulb that the branch scale is used is selected in the field
Select for 8～October the oriental hybrid lily plant of robust growth to give mark in the field;
3, burst
8～October, excavate the plant of having marked, select the healthy bulb of specification 14～22cm, clean the back and divide 5 layers of peripheral down scale, standby.
4, scale sterilization
Scale fully cleans with clear water earlier, soaked 15～30 minutes with the disinfectant that can effectively suppress mould (Penicillium spp.), fusarium (Fusarium spp.), botrytis sp (Botrytis spp.), rhizoctonia (Rhizoctoniaspp.), rotten mould (Pythium spp.) then, scale after the immersion is air-dry to the no moisture in surface at the environment of cleaning, and is standby;
5, embedding scale
Potassium permanganate solution with 0.04%～0.06% sprays the matrix after step (1) is handled, and makes matrix keep moistening; Be lined with in the kind ball Turnover Box of lily ball storage bag in scale after the sterilization and matrix 1: 2 by volume～4 packed into after mixing, storage bag is provided with air-vent, and sack is sealed;
6, thermal treatment
The Turnover Box that is embedded with scale is positioned over indoor heat-treating, require to handle place and extraneous air exchange amount hourly and should be 3～5 times that handle the place volume, keeping indoor temperature is 19～30 ℃, relative moisture 60%～80%, after under dark condition, handling 90～95 days, scale grows the following clove of differing in size of specification 5cm, should regularly water during the processing to keep matrix moistening;
7, cold treatment
After thermal treatment finishes, immediately Turnover Box is placed low temperature treatment under the dark condition, design temperature is 2～5 ℃ ± 0.5 ℃, handles 90～95 days, and regularly water make bury matrix maintenance moistening handling the thorough ventilation in place once every day during the cold treatment.
8, plantation of clove and management
After cold treatment finishes clove taken out from Turnover Box together with the scale that links to each other and plant in the seedbed, and technology is carried out seedbed management routinely, clove emerges after 14～30 days, grow the terrestrial stem plant, through 4～5 months growth, clove can be cultivated into satisfactory plantation female parent again.
Wherein the bulb described in the step 3 is the healthy bulb of 20～22cm.
Disinfectant described in the step 4 is the mixing suspension that contains carbendazim 0.4%, tpn 0.2%, sporgon 0.1%.
The Yin that further comprises 50ppm in the disinfectant described in the step 4 butyric acid of trembling.
In the step 5 with the moistening matrix of 0.05% potassium permanganate solution
The described lily ball storage bag of step 5 is the plastic sack that is provided with air-vent, air-vent diameter 0.6～1.0cm, pitch-row 15～25cm.
Heat treatment temperature described in the step 6 is 23～26 ℃ ± 1 ℃.
Cold treatment temperature described in the step 7 is 5 ℃ ± 0.5 ℃.
The present invention has overcome the deficiencies in the prior art, can control the plant development type of growing the clove that by the oriental hybrid lily scale effectively, significantly improve the ratio that it forms the terrestrial stem plant, make clove the plantation the plantation female parent that just can generate big specification in 1 year in a large number, shortened crop cycle, reduce production cost, had better market prospect.
Embodiment
Embodiment 1
The influence that scale under the bulb of investigation different size divides is grown clove.
1, the cultivation of clove
Select fresh peat soil in small, broken bits as matrix, standby after every cubic metre of matrix is mixed with 55% fenaminosulf pulvis 100g and 40% pcnb pulvis 100g.Select mid-September the oriental hybrid lily plant of robust growth to give mark in the field, late October the good plant of mark of gathering, select the healthy bulb of specification 14～16cm, 16～18cm, 20～22cm, divide kind and specification standby.The bulb flushing with clean water is divided 3 layers of scale of ecto-entad number down then.Scale under dividing fully cleans with clear water earlier, soaks sterilization in 15 minutes with the mixing suspension that contains carbendazim 0.4%, tpn 0.2%, sporgon 0.1% then, and the scale after the immersion is under the environment of cleaning, and is air-dry to the no moisture in surface, standby.Potassium permanganate solution with 0.05% sprays matrix, make matrix keep moistening, scale and matrix after the sterilization were mixed in 1: 3 by volume, be lined with in packing into then in the kind ball Turnover Box of lily ball storage bag, every case buries 1500 pieces of scales, and sack is sealed.Turnover Box is the mesh plastic box of the high 23cm of the wide 40cm of long 60cm, and storage bag is the plastic sack that is provided with air-vent, air-vent diameter 0.6～1.0cm, pitch-row 15～25cm.The Turnover Box that is embedded with scale is positioned in the special-purpose depots of flowering bulb heat-treats, requirement depots and extraneous air exchange amount hourly should be 3 times of its volume, keeping storehouse temperature is 23 ℃ ± 1 ℃, relative moisture 60%, under dark condition, handled 90 days, watered once to keep matrix moistening every 30 days during the processing.When thermal treatment finished, scale had produced the following clove of specification 5cm.At this moment, immediately Turnover Box is placed low temperature treatment under the dark condition, design temperature is 5 ℃ ± 0.5 ℃ to be handled 90 days, and regularly water make bury matrix keep moistening handling the thorough ventilation in place once every day during the cold treatment.The differentiation situation of clove saw Table 1 when cold treatment finished.
The clove specification and the quantity that produce when the scale cold treatment that the bulb of table 1 different size is told finishes
| Kind | Burst bulb specification (cm) | Handle scale quantity (sheet) | Clove specification (cm) and quantity (head) | Add up on (head) | Rotten scale quantity (sheet) | ||||
| Less than 1 | 1-2 | 2-3 | 3-4 | 4-5 | |||||
| Siberia Siberia | 14-16 | 1500 | 760 | 410 | 502 | 880 | 396 | 2948 | 58 |
| 16-18 | 1500 | 629 | 463 | 550 | 904 | 482 | 3028 | 57 | |
| 20-22 | 1500 | 405 | 602 | 601 | 936 | 653 | 3197 | 62 | |
| Sorbonne rope freshwater mussel | 14-16 | 1500 | 221 | 140 | 564 | 604 | 297 | 1826 | 73 |
| 16-18 | 1500 | 320 | 121 | 586 | 592 | 352 | 1971 | 70 | |
| 20-22 | 1500 | 365 | 295 | 550 | 637 | 390 | 2237 | 69 | |
2, plantation of clove and management
Plant in the seedbed after after cold treatment finishes clove being taken out from Turnover Box together with the scale that links to each other, planting environment is the plastic tunnel that disposes the dropper system, 6～13 ℃ of nighttime temperatures, 13～25 ℃ of daytimes; Nursery soil adopts paramo soil and humus soil composite soil, meadow soil and humus soil mixed proportion 1: 3, PH5.8; Top covering shading rate is 50% sunshade net.
Main field management measure: 400/m of planting density
2, degree of depth 5cm.The seedbed is wide 90cm, dark 3.5cm, the plantation row that length is determined according to each kind quantity.Smooth plantation row spreads fertilizer over the fields disinfectant, base fertilizer, waters, and evenly disseminates clove or have the scale of clove in plantation row, and blinding makes the furrow face concordant, waters permeable once more.Disinfectant be native bacterium go out the gram 11.5g/m
2, iron go out the gram 3g/m
2, phoxim baythion granule 1.2g/m
2The base fertilizer combination sees Table 2.
Table 2 fertilising table
| Fertilizer type | Every square metre of plantation row consumption (g) |
| Potassium dihydrogen phosphate | 11 |
| Potassium nitrate | 12 |
| Nitrate of lime | 6 |
| Epsom salt | 3 |
| Borax | 0.2 |
| Ferrous sulfate | 10 |
Later on every 40 days by this combination fertilising, totally 3 times, wherein ferrous sulfate has been executed for 1 time when using base fertilizer, does not use for back 3 times.Remaining is used by drip irrigation system.The back of emerging used that the 400ppm decis is anti-to eliminate aphis in per 20 days, and use 1000 times for per 10 days 1 time by turns and execute good happy 800 times of Sukelings and prevent and treat gray mold, and the maintenance ground moistening that often waters.Plantation the results are shown in Table 3.
The result of clove growth after 150 days that 1500 scales under table 3 different size bulb divides produce
| Kind | Burst bulb specification (cm) | Produce the scale quantity of clove | Clove sum (individual) during plantation | The terrestrial stem quantity (strain) that differentiates | Results specification (cm) and quantity (head) | |||
| 4-6 | 6-8 | 8-10 | 10-12 | |||||
| Siberia Siberia | 14-16 | 1442 | 2948 | 1202 | 987 | 580 | 337 | 480 |
| 16-18 | 1443 | 3028 | 1290 | 1163 | 812 | 400 | 487 | |
| 20-22 | 1438 | 3197 | 1449 | 1242 | 747 | 430 | 610 | |
| Sorbonne rope freshwater mussel | 14-16 | 1427 | 1826 | 964 | 762 | 740 | 239 | 28 |
| 16-18 | 1430 | 1971 | 1019 | 865 | 732 | 255 | 30 | |
| 20-22 | 1431 | 2237 | 1072 | 925 | 897 | 290 | 33 | |
Embodiment 2
Investigation under identical treatment conditions, the peripheral scale of oriental hybrid lily and difference by the scale of central main bud differentiation clove.
Repeat embodiment 1, following difference is arranged: select the healthy bulb of specification 16～18cm, divide kind standby.Clean the back and divide 5 layers of scale of ecto-entad number down, outmost 3 layers as the outer scale experimental group, two-layer as internal layer scale experimental group in addition, every group is provided with a repetition, 50 pieces on every group of scale.After thermal treatment finished, the situation of scale differentiation clove saw Table 4.
The difference of table 4 oriental hybrid lily internal layer and outer scale differentiation clove
| Kind | Experimental group | Specification (cm) and the quantity (head) of clove when thermal treatment finishes | Scale (sheet) rots during the thermal treatment | |||||
| Less than 1 | 1-2 | 2-3 | 3-4 | 4-5 | Add up on (head) | |||
| Acapulco marshal | Outer experimental group 1 | 19 | 43 | 54 | 58 | 3 | 177 | 1 |
| Internal layer experimental group 1 | 26 | 30 | 40 | 26 | 1 | 123 | 0 | |
| Outer experimental group 2 | 14 | 20 | 37 | 52 | 5 | 128 | 2 | |
| Internal layer experimental group 2 | 10 | 26 | 47 | 25 | 0 | 108 | 1 | |
| Siberia | Outer experimental group 1 | 16 | 42 | 26 | 43 | 8 | 135 | 2 |
| Siberia | Internal layer experimental group 1 | 4 | 11 | 29 | 36 | 2 | 82 | 1 |
| Outer experimental group 2 | 0 | 14 | 25 | 55 | 23 | 117 | 2 | |
| Internal layer experimental group 2 | 0 | 20 | 20 | 59 | 3 | 102 | 0 | |
| Sorbonne rope freshwater mussel | Outer experimental group 1 | 1 | 15 | 39 | 59 | 8 | 122 | 2 |
| Internal layer experimental group 1 | 7 | 26 | 29 | 35 | 4 | 101 | 1 | |
| Outer experimental group 2 | 3 | 10 | 34 | 58 | 13 | 118 | 4 | |
| Internal layer experimental group 2 | 9 | 39 | 25 | 21 | 3 | 97 | 0 |
Embodiment 3
Investigate of the influence of burst time to the differentiation of clove terrestrial stem.
Repeat embodiment 1, following difference is arranged: begin from mid-August, once sampled and burst every 20 days, each kind is gathered 40, selects 20 on the healthy bulb of specification 16～18cm wherein, divides kind standby, clean the back and divide 5 layers of scale of ecto-entad number down, experimental group is set in chronological order, and every group is provided with 1 repetition, every group of 50 pieces of scales.Scale after the cleaning soaks sterilization in 30 minutes with the mixing suspension that contains 0.4% carbendazim, 0.2% tpn, 0.1% sporgon.Cold treatment finishes the back by kind and experimental group classification, immediately clove is planted in the seedbed together with the scale that links to each other, and plantation the results are shown in Table 5.
The clove growth result that the scale of different burst times of table 5 produces
| The kind group | The burst time | Produce the scale quantity of clove | Clove sum (individual) during plantation | The terrestrial stem quantity (strain) that differentiates | Results specification (cm) and quantity (head) | |||
| 4-6 | 6-8 | 8-10 | 10-12 | |||||
| Acapulco repeats 1 | October 17 September 27 September 7 August 18 | 50 50 48 46 | 146 157 213 136 | 97 103 110 87 | 71 72 88 70 | 44 50 66 42 | 21 19 24 16 | 6 8 12 3 |
| Acapulco repeats 2 | October 17 September 27 September 7 August 18 | 50 50 50 44 | 160 141 197 133 | 105 98 102 90 | 75 75 90 78 | 58 40 72 35 | 10 17 20 12 | 8 5 9 2 |
| Siberia repeats 1 | October 17 September 27 September 7 August 18 | 50 50 50 48 | 157 150 124 108 | 95 93 67 58 | 59 57 65 63 | 43 44 32 27 | 19 27 11 10 | 33 18 9 5 |
| Siberia repeats 2 | August 18 | 50 50 50 47 | 151 157 132 108 | 101 108 70 57 | 64 67 36 60 | 30 27 65 29 | 23 35 21 11 | 28 20 5 4 |
| September 7 | ||||||||
| September 27 | ||||||||
| October 17 | ||||||||
| Sorbonne repeats 1 | October 17 September 27 September 7 August 18 | 50 50 50 42 | 172 210 177 146 | 100 96 102 61 | 76 100 85 81 | 68 62 68 46 | 21 30 27 14 | 0 0 |
| Sorbonne repeats 2 | October 17 September 27 September 7 August 18 | 50 50 50 41 | 181 194 195 143 | 116 119 95 63 | 70 80 96 81 | 74 73 63 50 | 25 27 24 7 | 5 7 4 1 |
Embodiment 4
Investigate of the influence of different heat treatment temperature to the clove differentiation terrestrial stem of oriental hybrid lily scale generation.
Repeat embodiment 1, following difference is arranged: late September is excavated the plant that mark is crossed, and each kind selects for use 40 on the healthy bulb of specification 16～18cm standby.After the bulb cleaning, divide bulb 3 layers of scale from outside to inside down, each kind selects 400 pieces on scale standby.Scale branch kind is imbedded matrix, and per 100 is 1 group.Use 20 ℃ ± 1 ℃, 23 ℃ ± 1 ℃, 26 ℃ ± 1 ℃, 29 ℃ ± 1 ℃ to handle respectively 90 days on embedded scale, the scale mean allocation of each kind is in each Temperature Treatment group.Cold treatment finishes the back by kind and heat treatment temperature classification, immediately clove is planted in the seedbed together with the scale that links to each other, and plantation the results are shown in Table 6.
The growth result of clove under the table 6 different heat treatment temperature
| Kind | Treatment temperature | The terrestrial stem quantity (strain) that differentiates | Results specification (cm) and quantity (head) | ||||
| 4-6 | 6-8 | 8-10 | 10-12 | Add up to | |||
| Acapulco marshal | 20℃±1 23℃±1?℃ | 51 123 | 151 144 | 127 141 | 51 49 | 9 30 | 338 364 |
| ?26℃±1℃ 29℃±1℃ | 207 29 | 108 186 | 136 120 | 100 34 | 55 7 | 399 347 | |
| Sorbonne rope freshwater mussel | 20℃±1℃ 23℃±1℃ 26℃±1℃ 29℃±1℃ | 62 95 116 50 | 127 107 112 114 | 92 98 87 96 | 43 50 64 35 | 5 25 30 8 | 267 280 293 253 |
| Tiber days despots | 20℃±1℃ 23℃±1℃ 26℃±1℃ 29℃±1℃ | 37 78 84 30 | 101 72 120 104 | 39 50 30 36 | 38 30 41 22 | 3 26 20 5 | 181 178 211 167 |
Embodiment 5
Investigate the different Yin influence of butyric acid density of trembling to oriental hybrid lily scale differentiation clove.
Repeat embodiment 1, following difference is arranged: excavate the plant that mark is crossed mid-September, each kind selects for use 10 on the healthy bulb of specification 16～18cm standby.After bulb cleaned, divide bulb 3 layers of scale from outside to inside down, each kind select 100 standby.In embodiment 1 described scale disinfectant, add Yin tremble butyric acid 1ppm, 10ppm, 50ppm, 100ppm respectively, be made into and contain tremble each 500ml of mixing suspension of butyric acid of Yin, and with embodiment 1 described disinfectant as the blank group.Scale divides kind, and per 20 is 1 group, and the scale quantity of each kind is evenly distributed in the above-mentioned various treatment fluid, soaks 15 minutes, drenches solid carbon dioxide and divides standby.When thermal treatment finished, the situation of scale differentiation clove saw Table 7.
The difference of oriental hybrid lily scale differentiation clove when the different Yin of table 7 tremble the butyric acid density processing
| Kind | Experimental group | Specification (cm) and the quantity (head) of clove when thermal treatment finishes | Scale (sheet) rots during the thermal treatment | |||||
| Less than 1 | 1-2 | 2-3 | 3-4 | 4-5 | Add up on (head) | |||
| Acapulco marshal | Blank 1ppm 10ppm | 15 11 14 | 14 24 37 | 33 27 38 | 22 7 9 | 0 0 3 | 84 69 101 | 0 2 0 |
| 50ppm 100ppm | 27 11 | 43 37 | 38 40 | 18 11 | 0 0 | 126 99 | 0 2 | |
| Siberia Siberia | Blank 1ppm 10ppm 50ppm 100ppm | 6 3 4 11 11 | 5 11 21 30 31 | 16 26 25 35 25 | 26 30 22 20 23 | 4 1 3 5 3 | 57 71 75 101 93 | 0 0 0 0 0 |
| Sorbonne rope freshwater mussel | Blank 1ppm 10ppm 50ppm 100ppm | 2 3 5 6 6 | 11 6 12 7 11 | 11 29 15 33 33 | 14 19 19 18 16 | 1 1 3 0 0 | 39 58 54 64 66 | 5 2 1 1 3 |
| Tiber days despots | Blank 1ppm 10ppm 50ppm 100ppm | 4 2 0 0 1 | 6 1 5 3 3 | 7 5 6 6 10 | 12 21 15 13 7 | 5 6 5 7 5 | 34 35 31 29 26 | 1 2 4 5 4 |
Embodiment 6
Investigate of the influence of different cold treatment temperature to the clove differentiation terrestrial stem of oriental hybrid lily scale generation.
Repeat embodiment 1, following difference is arranged: late September is excavated the plant that mark is crossed, and each kind selects for use 20 on the healthy bulb of specification 16～18cm standby.After the bulb cleaning, divide bulb 3 layers of scale from outside to inside down, each kind selects 200 on scale standby.Scale divides kind, and per 100 is 1 group, uses 5 ℃ ± 0.5 ℃, 2 ℃ ± 0.5 ℃ to handle during cold treatment respectively 90 days.Divide the kind mean allocation in above-mentioned processing on each kind scale.Cold treatment finishes the back by kind and experimental group classification, immediately clove is planted in the seedbed together with the scale that links to each other.Plantation the results are shown in Table 8.
The growth result of clove under the different cold treatment temperature of table 8
| Kind | Treatment temperature | The terrestrial stem quantity (strain) that differentiates | Results specification (cm) and quantity (head) | ||||
| 4-6 | 6-8 | 8-10 | 10-12 | Add up to | |||
| Acapulco marshal | 2℃±0.5℃ 5℃±0.5℃ | 64 180 | 176 162 | 85 112 | 29 37 | 4 16 | 294 327 |
| Siberia Siberia | 2℃±0.5℃ 5℃±0.5℃ | 69 169 | 150 130 | 77 72 | 34 46 | 31 57 | 292 305 |
| Sorbonne rope freshwater mussel | 2℃±0.5℃ 5℃±0.5℃ | 87 200 | 145 137 | 103 119 | 42 31 | 0 0 | 290 287 |
| Tiber days despots | 2℃±0.5℃ 5℃±0.5℃ | 39 129 | 85 70 | 89 90 | 35 32 | 0 10 | 209 202 |
Embodiment 7
Investigate the terrestrial stem quantity of the different size clove differentiation of oriental hybrid lily scale generation.
Repeat embodiment 1, following difference is arranged: cold treatment finishes the back and selects each 100 on the bulb of specification 2-3cm, 3-4cm, 4-5cm by kind, and clove is planted in the seedbed after scale takes off, and plantation the results are shown in Table 9.
The growth result of table 9 different size clove
| Kind | Clove specification (cm) | The terrestrial stem quantity (strain) that differentiates | Results specification (cm) and quantity (head) | ||||
| 4-6 | 6-8 | 8-10 | 10-12 | Add up to | |||
| Acapulco marshal | 2-3 3-4 4-5 | 12 97 100 | 70 0 0 | 23 4 1 | 5 87 51 | 0 9 48 | 98 100 100 |
| Siberia Siberia | 2-3 3-4 4-5 | 0 85 100 | 60 0 0 | 30 8 2 | 7 75 31 | 0 15 67 | 97 98 100 |
| Sorbonne rope freshwater mussel | 2-3 3-4 4-5 | 15 98 100 | 47 2 0 | 40 9 4 | 12 58 34 | 0 31 62 | 99 100 100 |
| Tiber days despots | 2-3 3-4 4-5 | 5 96 100 | 61 0 0 | 32 12 3 | 3 58 22 | 0 28 75 | 96 98 100 |
Claims (8)
1. the propagation method of a female parent of oriental lily, this method comprises the steps:
(1) matrix is prepared
Select fresh peat soil as matrix, the sterilization back is standby;
(2) bulb that the branch scale is used is selected in the field
Select for 8～October the oriental hybrid lily plant of robust growth to give mark in the field;
(3) burst
8～October, excavate the plant of having marked, select the healthy bulb of specification 14～22cm, clean the back and divide 5 layers of peripheral down scale, standby;
(4) scale sterilization
Scale fully cleans with clear water earlier, soaks 15～30 minutes with effectively suppressing mould, fusarium, botrytis sp, rhizoctonia, rotten mould disinfectant then, and the scale after the immersion is air-dry to the no moisture in surface at the environment of cleaning, and is standby;
(5) embedding scale
Potassium permanganate solution with 0.04%～0.06% sprays the matrix after step (1) is handled, and makes matrix keep moistening; Be lined with in the kind ball Turnover Box of lily ball storage bag in scale after the sterilization and matrix 1: 2 by volume～4 packed into after mixing, storage bag is provided with air-vent, and sack is sealed;
(6) thermal treatment
The Turnover Box that is embedded with scale is positioned over indoor heat-treating, require to handle place and extraneous air exchange amount hourly and should be 3～5 times that handle the place volume, keeping indoor temperature is 19～30 ℃, relative moisture 60%～80%, handled under dark condition 90～95 days, should regularly water during the processing keeps matrix moistening;
(7) cold treatment
After thermal treatment finishes, immediately Turnover Box is placed low temperature treatment under the dark condition, design temperature is 2～5 ℃ ± 0.5 ℃, handles 90～95 days, and regularly water make bury matrix maintenance moistening handling the thorough ventilation in place once every day during the cold treatment;
(8) plantation of clove and management
After cold treatment finishes clove taken out from Turnover Box together with the scale that links to each other and plant in the seedbed, and technology is carried out seedbed management routinely, clove emerges after 14～30 days, grow the terrestrial stem plant, through 4～5 months growth, clove can be cultivated into satisfactory plantation female parent again.
2. the propagation method of a kind of female parent of oriental lily according to claim 1, it is characterized in that: the bulb described in the step (3) is the healthy bulb of 20～22cm.
3. the propagation method of a kind of female parent of oriental lily according to claim 1, it is characterized in that: the disinfectant described in the step (4) is the mixing suspension that contains carbendazim 0.4%, tpn 0.2%, sporgon 0.1%.
4. according to the propagation method of claim 1 or 3 described a kind of female parent of oriental lily, it is characterized in that: the Yin that further comprises 50ppm in the disinfectant described in the step (4) butyric acid of trembling.
5. the propagation method of a kind of female parent of oriental lily according to claim 1 is characterized in that: in the step (5) with the moistening matrix of 0.05% potassium permanganate solution.
6. the propagation method of a kind of female parent of oriental lily according to claim 1, it is characterized in that: the described lily ball storage bag of step (5) is the plastic sack that is provided with air-vent, air-vent diameter 0.6～1.0cm, pitch-row 15～25cm.
7. the propagation method of a kind of female parent of oriental lily according to claim 1, it is characterized in that: the heat treatment temperature described in the step (6) is 23～26 ℃ ± 1 ℃.
8. the propagation method of a kind of female parent of oriental lily according to claim 1, it is characterized in that: the cold treatment temperature described in the step (7) is 5 ℃ ± 0.5 ℃.
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| CN101711493B (en) * | 2009-11-11 | 2013-01-02 | 玉溪明珠花卉股份有限公司 | Propagation of lily seedball by directly peeling scale |
| CN102626030B (en) * | 2012-04-25 | 2013-10-23 | 大理润森花卉开发有限公司 | Cultivation method for growing tissue-cultured Oriental lily bulbs in 18 months |
| CN103733832B (en) * | 2013-12-11 | 2015-10-14 | 甘肃大河中草药发展有限公司 | A kind of seedling-cultivating method of lanzhou lily |
| CN103782792A (en) * | 2014-03-12 | 2014-05-14 | 中国科学院昆明植物研究所 | Nomocharis plant scale bulb embedding and cultivating method |
| CN104304030B (en) * | 2014-10-31 | 2016-06-29 | 浙江大学宁波理工学院 | A kind of propagation method of oriental hybrid lily |
| CN104429497A (en) * | 2014-11-17 | 2015-03-25 | 成都红柿子科技有限公司 | Planting method for lilies |
| CN106489462A (en) * | 2015-09-07 | 2017-03-15 | 宁远县富鑫生态农业开发有限公司 | A kind of Lilium longiflorum rapid breeding method |
| CN107494224A (en) * | 2017-09-07 | 2017-12-22 | 东北农业大学 | A kind of bulb reproduction method of Lilium Pollyanna |
| CN115843645A (en) * | 2022-11-16 | 2023-03-28 | 云南省农业科学院花卉研究所 | Method for controlling germination quantity of lily scale seed balls and promoting quick expansion of seed balls |
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| CN1179260A (en) * | 1996-10-10 | 1998-04-22 | 谢祥华 | Cultivation and reproducing of Chinese medicinal crop lily on hill surface |
| CN1484939A (en) * | 2002-09-23 | 2004-03-31 | 杨贤志 | lily cultivation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1179260A (en) * | 1996-10-10 | 1998-04-22 | 谢祥华 | Cultivation and reproducing of Chinese medicinal crop lily on hill surface |
| CN1484939A (en) * | 2002-09-23 | 2004-03-31 | 杨贤志 | lily cultivation method |
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| 东方百合鳞片的组织培养. 唐东芹等.植物生理学通讯，第39卷第5期. 2003 * |
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