CN103782792A - Nomocharis plant scale bulb embedding and cultivating method - Google Patents
Nomocharis plant scale bulb embedding and cultivating method Download PDFInfo
- Publication number
- CN103782792A CN103782792A CN201410090815.3A CN201410090815A CN103782792A CN 103782792 A CN103782792 A CN 103782792A CN 201410090815 A CN201410090815 A CN 201410090815A CN 103782792 A CN103782792 A CN 103782792A
- Authority
- CN
- China
- Prior art keywords
- cuttage
- scale
- nomocharis
- cutting medium
- matrix
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
Provided is a nomocharis plant scale bulb embedding and cultivating method. Seed balls which are healthy, free of diseases, suitable for stripping and used for scale bulb embedding and cultivation and have the diameter ranging from 2cm to 3cm are selected, subjected to disinfection treatment with 1000 times of chlorothalonil for 15 minutes and then subjected to cottage in a mixed matrix with the volume ratio of straw earthy coal: humus soil: perlite = 1:1:1, the humidity is 30%-40%, and then the seed balls are put into a cultivating room at the constant temperature of 20+/-1 DEG C for cultivation. After 60 days, the average bud ratio can reach to higher than 80%, the average propagation coefficient can reach to higher than 1.5 (scale propagation coefficient = newly growing seed ball number cuttage scale number).
Description
Affiliated field:
The present invention relates to biological technical field, particularly, relate to ornamental flower Genus Nomocharis scale bag and bury and cultivation method.
Background technology:
Leopard Pittosporum (Nomocharis) be in Liliaceae (Liliaceae) lily family (Lilieae) with lilium relation very close a genus.This genus, as an endemic genus of Southeastern Tibet edge, is mainly distributed in northwestern Yunnan Province, Southeastern Tibet, western Sichuan and contiguous Upper Myanmar, the alpine belt of the western height above sea level 2900-4000m of Nepal.Totally 9 kinds of subordinates, Chinese branch has 7 kinds (N.georgei is distributed in Upper Myanmar, and N.oxypetala is distributed in Kashmir and Nepal western part).Leopard flower is Wild bulbar flower, perennial herb, the high 30-100 centimetre of stem, napiform root scale 9-10 piece.The florescence 6-7 month, the fruit phase 8-11 month.Pattern is gorgeous colourful, for good ornamental flower, is not yet developed in China, also can be used as fresh cut-flowers, because it wins liking of the zoophytologist of China and foreign countries compared with high ornamental value.From 1910, the Europe cultivation that successively successfully induced one of some kinds, and cultivate high, the adaptable garden-variety of some ornamental values.At present, Genus Nomocharis has become the ornamental flower of commonly seeing in Europe, America.But as the China in original producton location, about the relevant report of Genus Nomocharis introducing and planting and breeding research also fewer.Because its area is narrow, population quantity is little; some kinds have been listed in state guarantee plant, as beautiful leopard flower (N.basilissa), many spots leopard flower (N.meleagrina) (in part bibliographical information, green sieve leopard flower N.biluoensis is this kind of different name) etc.Genus Nomocharis breeding is main by excavating from field at present, serious to wild resource destruction, if it is too high to use tissue culture propagating cost instead, realize protection and the exploitation of leopard flower wild resource, and utilizing scale cuttage breeding method is a kind of effectively propagation method.By this technology, can realize the expanding propagation of this Wild Flowers, reduce reproductive-cost, protection wild resource, prevents exhaustive exploitation, for this wild resource sustainable use and development provides material base, scientific basis and technical support.
So far, in prior art, do not relate to the report of ornamental flower Genus Nomocharis scale ball embedding techniques and cultivation method.
Summary of the invention:
Object of the present invention aims to provide conveniently good ornamental flower Genus Nomocharis scale ball embedding techniques and cultivation method of one.The inventive method can realize the expanding propagation of leopard Pittosporum Wild Flowers, reduces reproductive-cost, and protection wild resource, prevents exhaustive exploitation, for the wild resource sustainable use of leopard Pittosporum and development provides material base, scientific basis and technical support.
The object of the invention is to be achieved by following technical scheme:
Genus Nomocharis scale bag buries and cultivation method, to select diameter disease-free Genus Nomocharis kind ball between 2-3cm, 6 scales that strip outer and middle level, scale cuttage after the tpn of 1000 times is disinfected 15min, then puts in culturing room and cultivates.
As described in method, be by scale cuttage in the thick cutting medium of 5cm, seeding row spacing 3X4cm, scale outside cuttage upward, then cover the cutting medium of one deck 3-4cm.
As described in method, cuttage bottom and the moisturizing of surrounding covering with plastic film, cutting medium keeps the humidity of 30%-40%, puts into the indoor cultivation of constant temperature culture, 20 ± 1 ℃ of temperature after cuttage finishes.
As described in method, selected matrix is peat composed of rotten mosses soil or perlite or sawdust or mixed-matrix, described mixed-matrix is peat composed of rotten mosses soil: humus soil: perlite=1:1:1.
Concrete method of the present invention can be generalized into: choose good disease-free Genus Nomocharis kind ball, strip 6 scales in outer and middle level, disinfect after 15min through the tpn of 1000 times, the cuttage of random choose scale in the plastic crate that fills cutting medium (the wide * of long * is high=60cm*40cm*20cm basket at the bottom of and surrounding be covered with mulch film), the cutting medium of 5cm is spread at the basket end, press the seeding row spacing of 3*4cm, the cuttage upward of scale outside, the cutting medium of rear covering one deck 3-4cm.Cuttage basket top covering with plastic film guarantees that humidity is 30%-40%, and cuttage basket should be put into the indoor cultivation of constant temperature culture of 20 ± 1 ℃.It is peat composed of rotten mosses soil that cutting medium should be chosen volume ratio: the mixed-matrix of humus soil: perlite=1:1:1 carries out cuttage.
Embodiment:
Further illustrate essentiality content of the present invention with embodiments of the invention below, but do not limit the present invention with this.
Embodiment 1:
One, materials and methods:
Tested in the 6-7 month in 2012 and carry out in proving ground, Kunming Inst. of Botany, Chinese Academy of Sciences of Lijiang County In Yunnan Province city Lijing alpine garden.Choose the beautiful leopard flower of state guarantee plant (N.basilissa), many spots leopard flower (N.meleagrina) as experiment kind, choose in the same size, even disease-free kind of ball.The mixed-matrix of selecting peat composed of rotten mosses soil, perlite, sawdust equal-volume to mix is cutting medium.Preferred volume ratio is peat composed of rotten mosses soil: the mixed-matrix of humus soil: perlite=1:1:1 carries out cuttage.Strip 6 scales in outer and middle level.Scale cuttage after the tpn of 1000 times is disinfected 15min.When cuttage, the cuttage of random choose scale is in filling the plastic crate of cutting medium, and the basket end and surrounding are covered with mulch film, and 5 cutting medium is spread by the seeding row spacing of 3X4cm in the basket end, the cuttage upward of scale outside, the cutting medium of rear covering one deck 3-4cm.Cuttage finishes plastic crate to be put into the indoor cultivation of constant temperature culture, 20 ± 1 ℃ of temperature afterwards.Totally 3 processing, 50 scales of each processing.Cuttage is the observed and recorded situation of sprouting after 20 days, adds up bud ratio, newborn seed nodule number and the seed bulb diameter of scale breeding 60 days time.
Two, results and analysis:
Two kinds of Genus Nomocharis scale bags of table 1 bury experimental result
Note: scale reproduction coefficient=newborn seed nodule number ÷ cuttage sheet number
As can be seen from Table 1, the reproduction coefficient of leopard flower scale is relevant with wild species characteristic.Due to interspecific difference impact, there is some difference for the time of sprouting, average reproduction coefficient and seed mean diameter of a ball, and the time many spots leopard of sprouting spends the shortest, beautiful leopard to spend the required time longer.There is certain inverse relation with seed bulb diameter in average reproduction coefficient, mainly due to the parent cuttage scale with respect to same, generates seed nodule number amount more, is dispensed to nutriment in each seed ball relatively less, and seed bulb diameter is just relatively little.
Embodiment 2:
One, materials and methods:
Tested in the 6-7 month in 2013 and carry out in proving ground, Kunming Inst. of Botany, Chinese Academy of Sciences of Lijiang County In Yunnan Province city Lijing alpine garden.Material is (beautiful leopard flower N.basilissa, many spots leopard are spent N.meleagrina, open lobe leopard flower N.aperta) three wild species that introduce a fine variety in field, in the same size, even disease-free kind of ball.Select different substrates, selected matrix is peat composed of rotten mosses soil, perlite, sawdust and mixed-matrix (peat composed of rotten mosses soil: humus soil: perlite=1:1:1).Select the kind ball of disease-free health, strip 6 scales in outer and middle level.Scale cuttage after the tpn of 1000 times is disinfected 15min.When cuttage, the cuttage of random choose scale (the wide * of long * high=60cm*40cm*20cm) basket end and surrounding in the plastic crate that fills cutting medium are covered with mulch film, 5 cutting medium is spread by the seeding row spacing of 3*4cm in the basket end, the cuttage upward of scale outside, the cutting medium of rear covering one deck 3-4cm, can 5～6 groups of scales of cuttage according to basket of above method, last top covering with plastic film moisturizing (matrix should keep 30%～40% humidity).Cuttage finishes plastic crate to be put into the indoor cultivation of constant temperature culture, 20 ± 1 ℃ of temperature afterwards.Totally 3 processing, 50 scales of each processing, repeat 2 times.Cuttage is the observed and recorded situation of sprouting after 20 days, adds up bud ratio, newborn seed nodule number and the seed bulb diameter of scale breeding 60 days time.
Two, results and analysis:
Genus Nomocharis fertility comparison under table 2 different substrates
Note: scale reproduction coefficient=newborn seed nodule number ÷ cuttage sheet number
As can be seen from Table 2, the relatively various plant propagation situations of leopard Pittosporum under different substrates, sawdust matrix is sprouted the time early, main because due to better with respect to other three kinds of matrix air capacity of soils.From bud ratio, because nutrition content is relatively low, perlite is starkly lower than other three kinds of matrix.Can find out from newborn seed nodule number and average reproduction coefficient, peat composed of rotten mosses soil and mixed-matrix are more conducive to the breeding of Genus Nomocharis, have higher reproduction coefficient, and wherein mixed mechanism effect are slightly excellent.Parent scale size when seed mean diameter of a ball depends primarily on cuttage, the diameter that larger parent generates seed ball is often larger; During due to cuttage embedding, in every kind of matrix, select scale in the same size, so newborn seed bulb diameter does not have notable difference.
Comprehensive known mixed-matrix is all better than other three kinds of matrix on the time of sprouting, bud ratio, newborn seed nodule number, average reproduction coefficient, can be used as the desirable matrix that leopard flower scale bag buries.
Cutting medium all has a significant impact scale bud ratio, newborn seed nodule number, scale reproduction coefficient, seed bulb diameter, the number of taking root, and depends primarily on physical arrangement and the trophic level of cutting medium.Ventilative, water penetration and water conservation, the nutrient preserving capability of cutting medium structure directly have influence on the sprouting of leopard flower scale, rooting rate and the healthy and strong degree of small seed ball.The mixed-matrix of peat composed of rotten mosses soil: humus soil: perlite=1:1:1 can be used as the cutting medium of leopard flower.The reproduction coefficient of leopard flower scale is relevant with wild species characteristic.The lepisphere diameter stem of the selected leopard flower of the present invention is 2-3cm, due to the restriction of scale nutrition content, therefore the reproduction coefficient of scale and seed bulb diameter are inversely proportional to.Due to interspecific difference, it is also different that different plant species seed ball produces quantity; Because seed mean diameter of a ball depends on cuttage parent scale size, in the time of cuttage, should select as far as possible the large and outside scale of bulb to cultivate.
After 60 days, average bud ratio can reach more than 80%, and average reproduction coefficient can reach more than 1.5 (scale reproduction coefficient=newborn seed nodule number ÷ cuttage sheet number).
Excellent effect of the present invention is mainly reflected in:
1, the analysis of system the result of the test of different substrates for leopard flower scale ball embedding techniques, screened the optimum matrix of suitable this technology, and explained the preferred reason of this matrix.
2, spend wild kind to carry out test relatively for different leopards, analyze and point out that wild species exist interspecific difference, while choosing seed ball, should be noted seed ball size and selection criteria.
3, the advantage of having evaluated leopard flower scale ball embedding techniques from multiple angle systematic comparisons, comprises the indexs such as the time of sprouting, bud ratio, newborn seed nodule number, average reproduction coefficient, the appraisement system while can be used for instructing correlation technique test to carry out.
4, cultivate cost with respect to tissue higher, the present invention can reduce the reproductive-cost of Genus Nomocharis, expanding propagation Wild Flowers; reduce reproductive-cost; protection wild resource, prevents exhaustive exploitation, for this wild resource sustainable use and development provides material base, scientific basis and technical support.
Claims (5)
1. Genus Nomocharis scale bag buries and cultivation method, select diameter disease-free Genus Nomocharis kind ball between 2-3cm, 6 scales that strip outer and middle level, scale cuttage after the tpn of 1000 times is disinfected 15min, then puts in culturing room and cultivates.
2. the method for claim 1, by scale cuttage in the thick cutting medium of 5cm, seeding row spacing 3X4cm, scale outside cuttage upward, then cover the cutting medium of one deck 3-4cm.
3. the method for claim 1, cuttage bottom and the moisturizing of surrounding covering with plastic film, cutting medium keeps the humidity of 30%-40%, puts into the indoor cultivation of constant temperature culture, 20 ± 1 ℃ of temperature after cuttage finishes.
4. the method for claim 1, selected matrix is peat composed of rotten mosses soil or perlite or sawdust or mixed-matrix, described mixed-matrix is peat composed of rotten mosses soil: humus soil: perlite=1:1:1.
5. the method for claim 1, it is characterized in that choosing good disease-free Genus Nomocharis kind ball, strip 6 scales in outer and middle level, disinfect after 15min through the tpn of 1000 times, the cuttage of random choose scale is in filling peat composed of rotten mosses soil or perlite or sawdust or mixed-matrix peat composed of rotten mosses soil: in the container of the cutting medium of humus soil: perlite=1:1:1, container bottom and surrounding are covered with mulch film, the cutting medium of 5cm is spread in bottom, press the seeding row spacing of 3X4cm, the cuttage upward of scale outside, cover again the cutting medium of one deck 3-4cm, cuttage vessel covering with plastic film guarantees that humidity is 30%-40%, then put into the indoor cultivation of constant temperature culture of 20 ± 1 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410090815.3A CN103782792A (en) | 2014-03-12 | 2014-03-12 | Nomocharis plant scale bulb embedding and cultivating method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410090815.3A CN103782792A (en) | 2014-03-12 | 2014-03-12 | Nomocharis plant scale bulb embedding and cultivating method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103782792A true CN103782792A (en) | 2014-05-14 |
Family
ID=50659181
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410090815.3A Pending CN103782792A (en) | 2014-03-12 | 2014-03-12 | Nomocharis plant scale bulb embedding and cultivating method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103782792A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1736151A (en) * | 2005-07-26 | 2006-02-22 | 云南格桑花卉有限责任公司 | Method for propagating female parent of oriental lily |
| CN101536645A (en) * | 2009-04-29 | 2009-09-23 | 云南省农业科学院花卉研究所 | Method for rapid cultivation of lily bud scale bulb-lets |
| CN101711493A (en) * | 2009-11-11 | 2010-05-26 | 玉溪明珠花卉股份有限公司 | Propagation of lily seedball by directly peeling scale |
| CN102972198A (en) * | 2012-12-25 | 2013-03-20 | 连云港市农业科学院 | Method for enlarging oriental lily scale cutting ball |
-
2014
- 2014-03-12 CN CN201410090815.3A patent/CN103782792A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1736151A (en) * | 2005-07-26 | 2006-02-22 | 云南格桑花卉有限责任公司 | Method for propagating female parent of oriental lily |
| CN101536645A (en) * | 2009-04-29 | 2009-09-23 | 云南省农业科学院花卉研究所 | Method for rapid cultivation of lily bud scale bulb-lets |
| CN101711493A (en) * | 2009-11-11 | 2010-05-26 | 玉溪明珠花卉股份有限公司 | Propagation of lily seedball by directly peeling scale |
| CN102972198A (en) * | 2012-12-25 | 2013-03-20 | 连云港市农业科学院 | Method for enlarging oriental lily scale cutting ball |
Non-Patent Citations (1)
| Title |
|---|
| 刘维暐等: "豹子花属植物鳞片扦插繁殖的研究", 《北方园艺》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105993804B (en) | Method for cultivating citrus container mycorrhizal seedlings by utilizing wormcast to prepare nutrient soil | |
| CN103814652B (en) | The seed germination method of Yunnan Paris polyphylla and gross weight building plant | |
| CN103145497B (en) | Novel mushroom residue and arbuscular mycorrhizal fungi (AMF) culture medium, and preparation method and application thereof | |
| Karlsson et al. | Effect of cultivar, traditional corm pre-treatment and watering on sprouting and early growth of enset (Ensete ventricosum) suckers | |
| CN105684849A (en) | Organic substrate containing arbuscular mycorrhiza fungi and preparing method and application thereof | |
| Bertucci et al. | Comparison of root system morphology of cucurbit rootstocks for use in watermelon grafting | |
| Janket et al. | Genetic diversity of water use efficiency in Jerusalem artichoke ('Helianthus tuberosus' L.) germplasm | |
| Oagile et al. | Effect of container size on the growth and development of tomato seedlings | |
| Gonzales et al. | Response of Pechay (Brassica napus L.) to different levels of compost fertilizer | |
| Kim et al. | Growth response and developing simple test method for waterlogging stress tolerance in soybean | |
| CN103444415B (en) | Breeding seedling-growing method for polygonum capitatum of Relinqing granule medicine materials | |
| Mabhaudhi et al. | Growth response of selected taro [Colocasia esculenta (L.) schott] landraces to water stress | |
| CN105393672B (en) | A kind of method effectively abolished husky honey raisin tree seed dormancy, promote emergence | |
| Osoro et al. | Effects of water hyacinth (Eichhornia crassipes [mart.] solms) compost on growth and yield of common beans (Phaseolus vulgaris) in Lake Victoria Basin | |
| Alawathugoda et al. | Effects of mycorrhizae as a substitute for inorganic fertilizer on growth and yield of tomato (Lycopersicon esculentum L.) and soybean (Glycine max L.), and soil microbial activity | |
| CN104016755A (en) | Vinegar residue substrate for cultivating cherry tomatoes | |
| CN103229694A (en) | Method for improving survival rate of trees in planting stage in arid areas | |
| CN106211862A (en) | A kind of Acer buergerianum Miq． presprouting of seeds and seeding growing seedlings method | |
| Soldan et al. | Development of forestry species inoculated with Trichoderma spp. Fertilized with rock phosphate | |
| CN103782792A (en) | Nomocharis plant scale bulb embedding and cultivating method | |
| Coelho et al. | Utilization and fate of phosphorus of different sources applied to cohesive soil of Amazonian periphery | |
| Roy et al. | Effect of organic growing media and crop geometry on growth and yield of capsicum var. California wonder under protected condition in North West Himalayas | |
| Singh et al. | Grain yield, water use efficiency, economics and soil moisture extraction pattern of summer greengram (Vigna radiata var. radiata) as influenced by planting and irrigation methods; Irrigation schedules and vam inoculation | |
| CN109122116A (en) | A kind of high-efficient culture method of wheat seedling test material | |
| Hamma et al. | Effect of irrigation intervals and NPK application on the growth and yield of carrot (Daucus carota L.) in samaru, kaduna state, Nigeria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140514 |