CL2012002087A1 - Metodo intracelular de conversion de una proteina de cadena simple de toxina de clostridium, en su forma dicatenaria. - Google Patents

Metodo intracelular de conversion de una proteina de cadena simple de toxina de clostridium, en su forma dicatenaria.

Info

Publication number
CL2012002087A1
CL2012002087A1 CL2012002087A CL2012002087A CL2012002087A1 CL 2012002087 A1 CL2012002087 A1 CL 2012002087A1 CL 2012002087 A CL2012002087 A CL 2012002087A CL 2012002087 A CL2012002087 A CL 2012002087A CL 2012002087 A1 CL2012002087 A1 CL 2012002087A1
Authority
CL
Chile
Prior art keywords
conversion
dicarious
single chain
clostridium toxin
chain protein
Prior art date
Application number
CL2012002087A
Other languages
English (en)
Inventor
Sanjiv Ghanshani
Linh Q Le
Yi Lui
Lance E Steward
Original Assignee
Allergan Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Allergan Inc filed Critical Allergan Inc
Publication of CL2012002087A1 publication Critical patent/CL2012002087A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Método de intracelular de conversión de toxina de clostridium de cadena simple que comprende una región de bucle dicatenario en su forma dicatenaria.
CL2012002087A 2010-01-25 2012-07-25 Metodo intracelular de conversion de una proteina de cadena simple de toxina de clostridium, en su forma dicatenaria. CL2012002087A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US28696310P 2010-01-25 2010-01-25

Publications (1)

Publication Number Publication Date
CL2012002087A1 true CL2012002087A1 (es) 2013-01-11

Family

ID=44146329

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2012002087A CL2012002087A1 (es) 2010-01-25 2012-07-25 Metodo intracelular de conversion de una proteina de cadena simple de toxina de clostridium, en su forma dicatenaria.

Country Status (24)

Country Link
US (3) US8546108B2 (es)
EP (3) EP3034511A1 (es)
JP (2) JP5956350B2 (es)
KR (1) KR101930962B1 (es)
CN (1) CN102822196B (es)
AU (1) AU2016204285B2 (es)
BR (1) BR112012018526A2 (es)
CA (1) CA2788074C (es)
CL (1) CL2012002087A1 (es)
CY (2) CY1115341T1 (es)
DK (1) DK2528940T3 (es)
ES (2) ES2592856T3 (es)
HK (1) HK1179634A1 (es)
HU (1) HUE029962T2 (es)
IL (2) IL221095A (es)
MX (1) MX2012008650A (es)
NZ (1) NZ601472A (es)
PL (2) PL2684890T3 (es)
PT (2) PT2528940E (es)
RU (1) RU2569185C2 (es)
SG (1) SG182691A1 (es)
SI (2) SI2684890T1 (es)
WO (1) WO2011091370A1 (es)
ZA (1) ZA201205793B (es)

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CN111019926B (zh) * 2018-10-10 2021-11-26 上饶市康可得生物科技有限公司 Tev蛋白酶变体、其融合蛋白及制备方法和用途
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CN111019925B (zh) * 2018-10-10 2022-08-23 上饶市康可得生物科技有限公司 筛选蛋白酶变体的方法以及获得的蛋白酶变体
JP7296660B2 (ja) * 2018-10-10 2023-06-23 上饒市康可得生物科技有限公司 Ｔｅｖプロテアーゼバリアント、その融合タンパク質、調製方法および使用
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HK1179634A1 (en) 2013-10-04
PT2528940E (pt) 2014-06-24
CA2788074C (en) 2016-07-12
AU2011207445A1 (en) 2012-08-30
ES2592856T3 (es) 2016-12-01
US8546108B2 (en) 2013-10-01
HUE029962T2 (hu) 2017-04-28
CN102822196B (zh) 2014-12-17
EP2684890A1 (en) 2014-01-15
IL246310A0 (en) 2016-07-31
EP2528940B1 (en) 2014-05-14
SI2684890T1 (sl) 2016-10-28
MX2012008650A (es) 2012-11-23
PL2528940T3 (pl) 2014-10-31
CA2788074A1 (en) 2011-07-28
CN102822196A (zh) 2012-12-12
KR20120134117A (ko) 2012-12-11
BR112012018526A2 (pt) 2020-09-08
US9938514B2 (en) 2018-04-10
US20140030759A1 (en) 2014-01-30
RU2012136227A (ru) 2014-03-10
IL246310B (en) 2018-11-29
JP5956350B2 (ja) 2016-07-27
KR101930962B1 (ko) 2018-12-19
JP2016164205A (ja) 2016-09-08
JP6312745B2 (ja) 2018-04-18
RU2569185C2 (ru) 2015-11-20
US20120295330A1 (en) 2012-11-22
EP2528940A1 (en) 2012-12-05
WO2011091370A1 (en) 2011-07-28
EP3034511A1 (en) 2016-06-22
EP2684890B1 (en) 2016-04-20
ZA201205793B (en) 2013-05-29
AU2016204285A1 (en) 2016-07-14
PL2684890T3 (pl) 2016-11-30
IL221095A (en) 2017-06-29
PT2684890T (pt) 2016-07-28
NZ601472A (en) 2013-08-30
CY1115341T1 (el) 2017-01-04
US9340813B2 (en) 2016-05-17
JP2013517797A (ja) 2013-05-20
US20160319265A1 (en) 2016-11-03
AU2016204285B2 (en) 2018-03-29
ES2488217T3 (es) 2014-08-26
SI2528940T1 (sl) 2014-07-31
SG182691A1 (en) 2012-08-30
CY1117797T1 (el) 2017-05-17
DK2528940T3 (da) 2014-07-14

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