CY1117797T1 - Μεθοδοι ενδοκυτταρικης μετατροπης πρωτεϊνων μονης αλυσιδας προς τη μορφη διπλης αλυσιδας αυτων - Google Patents

Μεθοδοι ενδοκυτταρικης μετατροπης πρωτεϊνων μονης αλυσιδας προς τη μορφη διπλης αλυσιδας αυτων

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Publication number
CY1117797T1
CY1117797T1 CY20161100700T CY161100700T CY1117797T1 CY 1117797 T1 CY1117797 T1 CY 1117797T1 CY 20161100700 T CY20161100700 T CY 20161100700T CY 161100700 T CY161100700 T CY 161100700T CY 1117797 T1 CY1117797 T1 CY 1117797T1
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CY
Cyprus
Prior art keywords
methods
intellacycular
double chain
single chain
double
Prior art date
Application number
CY20161100700T
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English (en)
Inventor
Sanjiv Ghanshani
Linh Q Le
Yi Liu
Lance E Steward
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Allergan, Inc.
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Publication date
Application filed by Allergan, Inc. filed Critical Allergan, Inc.
Publication of CY1117797T1 publication Critical patent/CY1117797T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
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    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Η παρούσα περιγραφή αποκαλύπτει κατασκευάσματα έκφρασης τα οποία περιλαμβάνουν πρωτεΐνες μονής αλυσίδας, οι οποίες περιλαμβάνουν μία περιοχή βρόχου διπλής αλυσίδας η οποία περιλαμβάνει μία εξωγενή θέση διάσπασης πρωτεάσης και μία πρωτεάση η οποία μπορεί να διασπά την εξωγενή θέση διάσπασης πρωτεάσης η οποία βρίσκεται εντός του βρόχου διπλής αλυσίδας, κυτταρικές συνθέσεις οι οποίες περιλαμβάνουν τέτοιο κατασκεύασμα έκφρασης και ενδοκυτταρικές μεθόδους μετατροπής της πρωτεΐνης μονής αλυσίδας προς τη μορφή διπλής αλυσίδας αυτής.
CY20161100700T 2010-01-25 2016-07-19 Μεθοδοι ενδοκυτταρικης μετατροπης πρωτεϊνων μονης αλυσιδας προς τη μορφη διπλης αλυσιδας αυτων CY1117797T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28696310P 2010-01-25 2010-01-25
EP11702755.7A EP2528940B1 (en) 2010-01-25 2011-01-24 Methods of intracellular conversion of single-chain proteins into their di-chain form

Publications (1)

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CY1117797T1 true CY1117797T1 (el) 2017-05-17

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CY20141100528T CY1115341T1 (el) 2010-01-25 2014-07-11 Μεθοδοι ενδοκυτταρικης μετατροπης πρωτεϊνων μονης αλυσιδας προς τη μορφη διπλης αλυσιδας αυτων
CY20161100700T CY1117797T1 (el) 2010-01-25 2016-07-19 Μεθοδοι ενδοκυτταρικης μετατροπης πρωτεϊνων μονης αλυσιδας προς τη μορφη διπλης αλυσιδας αυτων

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US (3) US8546108B2 (el)
EP (3) EP2684890B1 (el)
JP (2) JP5956350B2 (el)
KR (1) KR101930962B1 (el)
CN (1) CN102822196B (el)
AU (1) AU2016204285B2 (el)
BR (1) BR112012018526A2 (el)
CA (1) CA2788074C (el)
CL (1) CL2012002087A1 (el)
CY (2) CY1115341T1 (el)
DK (1) DK2528940T3 (el)
ES (2) ES2592856T3 (el)
HK (1) HK1179634A1 (el)
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US20140030759A1 (en) 2014-01-30
IL246310B (en) 2018-11-29
SG182691A1 (en) 2012-08-30
PT2684890T (pt) 2016-07-28
CL2012002087A1 (es) 2013-01-11
AU2011207445A1 (en) 2012-08-30
HK1179634A1 (en) 2013-10-04
BR112012018526A2 (pt) 2020-09-08
DK2528940T3 (da) 2014-07-14
CY1115341T1 (el) 2017-01-04
EP2684890A1 (en) 2014-01-15
NZ601472A (en) 2013-08-30
EP3034511A1 (en) 2016-06-22
ES2488217T3 (es) 2014-08-26
US9938514B2 (en) 2018-04-10
PL2528940T3 (pl) 2014-10-31
CA2788074A1 (en) 2011-07-28
WO2011091370A1 (en) 2011-07-28
PL2684890T3 (pl) 2016-11-30
ZA201205793B (en) 2013-05-29
IL221095A (en) 2017-06-29
SI2684890T1 (sl) 2016-10-28
JP2013517797A (ja) 2013-05-20
KR101930962B1 (ko) 2018-12-19
EP2528940A1 (en) 2012-12-05
CN102822196B (zh) 2014-12-17
AU2016204285B2 (en) 2018-03-29
JP6312745B2 (ja) 2018-04-18
US20120295330A1 (en) 2012-11-22
PT2528940E (pt) 2014-06-24
JP2016164205A (ja) 2016-09-08
EP2528940B1 (en) 2014-05-14
CA2788074C (en) 2016-07-12
US8546108B2 (en) 2013-10-01
EP2684890B1 (en) 2016-04-20
MX2012008650A (es) 2012-11-23
KR20120134117A (ko) 2012-12-11
RU2569185C2 (ru) 2015-11-20
IL246310A0 (en) 2016-07-31
AU2016204285A1 (en) 2016-07-14
SI2528940T1 (sl) 2014-07-31
HUE029962T2 (hu) 2017-04-28
CN102822196A (zh) 2012-12-12
ES2592856T3 (es) 2016-12-01
US9340813B2 (en) 2016-05-17
RU2012136227A (ru) 2014-03-10
JP5956350B2 (ja) 2016-07-27
US20160319265A1 (en) 2016-11-03

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