CL2012001117A1 - Composicion que comprende celulas madres multipotenciales del arbol biliar de un mamífero; método para obtener células multipotentes de un mamífero capaz de diferenciarse en multiples linajes endotérmicos. - Google Patents

Composicion que comprende celulas madres multipotenciales del arbol biliar de un mamífero; método para obtener células multipotentes de un mamífero capaz de diferenciarse en multiples linajes endotérmicos.

Info

Publication number
CL2012001117A1
CL2012001117A1 CL2012001117A CL2012001117A CL2012001117A1 CL 2012001117 A1 CL2012001117 A1 CL 2012001117A1 CL 2012001117 A CL2012001117 A CL 2012001117A CL 2012001117 A CL2012001117 A CL 2012001117A CL 2012001117 A1 CL2012001117 A1 CL 2012001117A1
Authority
CL
Chile
Prior art keywords
mammal
lineages
differentiating
composition
cells
Prior art date
Application number
CL2012001117A
Other languages
English (en)
Spanish (es)
Inventor
Lola M Reid
Yunfang Wang
Vicenzo Cardinale
Eugenio Gaudio
Guido Carpino
Domenico Alvaro
Cal-Bin Cui
Original Assignee
The Univ Of North Carolina At Chapel Hill Y Sapienza Univ Di Roma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43922533&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CL2012001117(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by The Univ Of North Carolina At Chapel Hill Y Sapienza Univ Di Roma filed Critical The Univ Of North Carolina At Chapel Hill Y Sapienza Univ Di Roma
Publication of CL2012001117A1 publication Critical patent/CL2012001117A1/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/413—Gall bladder; Bile
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C12N5/068—Stem cells; Progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/335—Glucagon; Glucagon-like peptide [GLP]; Exendin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/80—Hyaluronan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2537/00—Supports and/or coatings for cell culture characterised by physical or chemical treatment
    • C12N2537/10—Cross-linking

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
CL2012001117A 2009-10-30 2012-04-27 Composicion que comprende celulas madres multipotenciales del arbol biliar de un mamífero; método para obtener células multipotentes de un mamífero capaz de diferenciarse en multiples linajes endotérmicos. CL2012001117A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US25684609P 2009-10-30 2009-10-30

Publications (1)

Publication Number Publication Date
CL2012001117A1 true CL2012001117A1 (es) 2013-03-22

Family

ID=43922533

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2012001117A CL2012001117A1 (es) 2009-10-30 2012-04-27 Composicion que comprende celulas madres multipotenciales del arbol biliar de un mamífero; método para obtener células multipotentes de un mamífero capaz de diferenciarse en multiples linajes endotérmicos.

Country Status (20)

Country Link
US (1) US20110135610A1 (enExample)
EP (2) EP3725874A1 (enExample)
JP (2) JP5936547B2 (enExample)
KR (4) KR20120117758A (enExample)
CN (2) CN105456292B (enExample)
AR (2) AR078849A1 (enExample)
AU (1) AU2016210783B2 (enExample)
BR (1) BR112012009848B1 (enExample)
CA (2) CA3169831C (enExample)
CL (1) CL2012001117A1 (enExample)
CR (1) CR20120254A (enExample)
ES (1) ES2819028T3 (enExample)
IL (2) IL219454A (enExample)
MX (1) MX2012005087A (enExample)
NZ (3) NZ709124A (enExample)
PH (1) PH12012500845A1 (enExample)
RU (2) RU2580246C2 (enExample)
SG (2) SG10201407102QA (enExample)
TW (3) TWI686478B (enExample)
WO (1) WO2011053690A1 (enExample)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3169831C (en) * 2009-10-30 2025-11-18 Sapienza Universita Di Roma Pluripodent Stem Cells from the Extrahepatic Biliary Tree and Methods for Their Isolation
AU2012272586B2 (en) * 2011-06-23 2016-07-07 The Children's Hospital Of Philadelphia Self-renewing endodermal progenitor lines generated from human pluripotent stem cells and methods of use thereof
US9533013B2 (en) 2013-03-13 2017-01-03 University Of North Carolina At Chapel Hill Method of treating pancreatic and liver conditions by endoscopic-mediated (or laparoscopic-mediated) transplantation of stem cells into/onto bile duct walls of particular regions of the biliary tree
CN103266084B (zh) * 2013-05-27 2015-03-04 刘卫辉 富集干细胞的三步分离法
WO2015142288A1 (en) * 2014-03-17 2015-09-24 Agency For Science, Technology And Research METHOD FOR PREDICTING TOXICITY OF A COMPOUND BASED ON NUCLEAR FACTOR-κB TRANSLOCATION
GB201507894D0 (en) * 2015-05-08 2015-06-24 Imagen Biotech Ltd Personalised media
GB201510950D0 (en) 2015-06-22 2015-08-05 Cambridge Entpr Ltd In vitro Production of Cholangiocytes
AU2016344599B2 (en) * 2015-10-30 2020-12-17 Biolamina Ab Methods for producing hepatocytes
GB201521179D0 (en) 2015-12-01 2016-01-13 Univ Leuven Kath Methods for differentiating cells into hepatic stellate cells
CN105807053B (zh) * 2016-04-15 2019-04-02 苏州药明康德新药开发股份有限公司 一种肿瘤解离试剂在流式细胞检测中的应用
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
WO2018187439A1 (en) * 2017-04-06 2018-10-11 The University Of North Carolina At Chapel Hill Cryopreservation method
IL277552B2 (en) * 2018-03-29 2024-08-01 Univ North Carolina Chapel Hill Stem/progenitor cells from Brunner's duodenal glands and methods for their isolation and use
CN112126618B (zh) * 2019-06-24 2023-09-12 上海拜羡生物科技有限公司 一种人胆囊干细胞的获取和长期体外培养的方法
CN113388569B (zh) * 2020-08-28 2022-05-17 广东乾晖生物科技有限公司 肝脏类器官的制备方法
AU2021453264A1 (en) * 2021-06-30 2023-12-14 Polbionica Sp. Z O.O. A medium for storing isolated pancreatic islets and a method for storing isolated pancreatic islets
CN115975913B (zh) * 2023-02-03 2024-06-04 北京中医药大学深圳医院(龙岗) 一种利用多能干细胞诱导分化胆囊祖细胞的方法

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3646197A (en) * 1969-05-14 1972-02-29 Zaidan Hojin Biseibutsu Novel process for removing copper from copper-containing bleomycin
US6069005A (en) * 1991-08-07 2000-05-30 Albert Einstein College Of Medicine Of Yeshwa University Hapatoblasts and method of isolating same
US5660207A (en) * 1994-12-29 1997-08-26 Tylan General, Inc. Flow controller, parts of flow controller, and related method
US5861313A (en) * 1995-06-07 1999-01-19 Ontogeny, Inc. Method of isolating bile duct progenitor cells
GB9605808D0 (en) * 1996-03-20 1996-05-22 Isis Innovation CFTR gene regulator
US5830850A (en) * 1996-08-28 1998-11-03 Mount Sinai School Of Medicine Of The City Of New York Methods for the treatment of bone resorption disorders, including osteoporosis
HK1046930B (zh) * 1999-01-19 2012-08-10 查珀尔希尔北卡罗来纳大学 人肝脏祖先
US7456017B2 (en) * 1999-10-01 2008-11-25 University Of North Carolina At Chapel Hill Processes for clonal growth of hepatic progenitor cells
ATE328067T1 (de) * 2000-04-28 2006-06-15 Childrens Medical Center Isolierung von mesenchymalen stammzellen und deren verwendung
US6828145B2 (en) * 2000-05-10 2004-12-07 Cedars-Sinai Medical Center Method for the isolation of stem cells by immuno-labeling with HLA/MHC gene product marker
JP3602038B2 (ja) * 2000-07-24 2004-12-15 株式会社日立グローバルストレージテクノロジーズ 磁気ヘッドおよび磁気記録再生装置
DE10064804C2 (de) * 2000-12-22 2003-03-20 Schott Glas Alkalifreie Aluminoborosilicatgläser und ihre Verwendung
AU2002340897A1 (en) 2001-09-12 2003-03-24 Develogen Aktiengesellschaft Fur Entwicklungsbiologische Forschung A method for isolating, culturing and differentiating intestinal stem cells for therapeutic use
US7150990B2 (en) * 2002-03-06 2006-12-19 Reprocell, Inc. Self-renewing pluripotent hepatic stem cells
ES2569951T3 (es) * 2002-03-15 2016-05-13 University Of North Carolina At Chapel Hill Células madre hepáticas primitivas y proximales
WO2004071464A2 (en) * 2003-02-12 2004-08-26 Johns Hopkins University School Of Medicine Diagnostic application of differentially-expressed genes in lympho-hematopoietic stem cells
DE10336152B4 (de) * 2003-08-06 2007-02-15 Nephrogen LLC, Salt Lake City Aufreinigungsverfahren für humane mesenchymale Stammzellen
US8119774B2 (en) * 2003-10-09 2012-02-21 Martin Pera Cell marker for hepatic and pancreatic stem cells and progenitor cells
KR20070007030A (ko) * 2003-10-28 2007-01-12 카리타스 세인트 엘리자베스 메디칼 센터 오브 보스톤, 인코포레이티드 신규한 다능성 줄기세포 및 그의 용도
US7081582B2 (en) * 2004-06-30 2006-07-25 Microsoft Corporation System and method for aligning and mixing songs of arbitrary genres
DK1838843T3 (da) 2004-12-23 2019-08-26 Viacyte Inc Ekspansion af definitive endodermceller
JP4773777B2 (ja) * 2005-08-30 2011-09-14 グローバル・オーエルイーディー・テクノロジー・リミテッド・ライアビリティ・カンパニー アクティブマトリクス型表示装置
SG169324A1 (en) * 2005-10-14 2011-03-30 Univ Minnesota Differentiation of non-embryonic stem cells to cells having a pancreatic phenotype
JP2009515558A (ja) * 2005-11-16 2009-04-16 ユニバーシティ オブ ノース カロライナ アット チャペル ヒル 肝前駆細胞の増殖又は分化用細胞外マトリックス成分
US9005964B2 (en) * 2006-11-24 2015-04-14 Regents Of The University Of Minnesota Endodermal progenitor cells
WO2008109659A2 (en) * 2007-03-06 2008-09-12 University Of North Carolina At Chapel Hill Complexes of hyaluronans, other matrix components, hormones and growth factors for maintenance, expansion and/or differentiation of cells
JP2008307007A (ja) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
KR20100094970A (ko) * 2007-06-15 2010-08-27 유니버시티 오브 노스캐롤라이나 앳 채플 힐 중간엽 공급자 세포로부터의 파라크린 시그널 및 이를 이용한 간 전구세포의 확장 및 분화 조절
CN101952415B (zh) * 2007-07-31 2017-06-27 生命扫描有限公司 人胚胎干细胞的分化
CA3169831C (en) * 2009-10-30 2025-11-18 Sapienza Universita Di Roma Pluripodent Stem Cells from the Extrahepatic Biliary Tree and Methods for Their Isolation
US10135700B2 (en) 2014-03-10 2018-11-20 Softphone SRL Enterprise application integration on client computing devices
US10387547B2 (en) 2014-09-09 2019-08-20 Intentional Software Corporation Layout engine for creating a visual layout tree for a document

Also Published As

Publication number Publication date
EP2494033A1 (en) 2012-09-05
TWI740180B (zh) 2021-09-21
MX2012005087A (es) 2012-09-28
WO2011053690A1 (en) 2011-05-05
RU2016104823A3 (enExample) 2019-08-19
RU2580246C2 (ru) 2016-04-10
TW201831678A (zh) 2018-09-01
CA2779144A1 (en) 2011-05-05
NZ724435A (en) 2021-02-26
ES2819028T3 (es) 2021-04-14
CA2779144C (en) 2022-10-25
IL219454A (en) 2017-05-29
SG10201913006XA (en) 2020-02-27
CN102918145A (zh) 2013-02-06
NZ600068A (en) 2014-05-30
KR20190065476A (ko) 2019-06-11
AU2016210783A1 (en) 2016-09-01
IL219454A0 (en) 2012-06-28
TWI641688B (zh) 2018-11-21
AR122249A2 (es) 2022-08-31
EP2494033B1 (en) 2020-07-01
JP6356165B2 (ja) 2018-07-11
EP2494033A4 (en) 2015-07-29
JP5936547B2 (ja) 2016-06-22
CR20120254A (es) 2012-09-24
NZ622427A (en) 2015-10-30
NZ709124A (en) 2017-06-30
TWI686478B (zh) 2020-03-01
RU2756584C2 (ru) 2021-10-01
SG10201407102QA (en) 2014-11-27
KR20120117758A (ko) 2012-10-24
KR20180055931A (ko) 2018-05-25
EP3725874A1 (en) 2020-10-21
KR20200146043A (ko) 2020-12-31
RU2016104823A (ru) 2018-11-22
JP2013509187A (ja) 2013-03-14
BR112012009848A2 (pt) 2015-09-15
TW202022111A (zh) 2020-06-16
BR112012009848B1 (pt) 2021-11-03
KR102016870B1 (ko) 2019-08-30
US20110135610A1 (en) 2011-06-09
CA3169831C (en) 2025-11-18
TW201122108A (en) 2011-07-01
RU2012121428A (ru) 2013-12-10
CN105456292A (zh) 2016-04-06
PH12012500845A1 (en) 2013-01-07
CN102918145B (zh) 2016-01-27
IL251763A0 (en) 2017-06-29
AU2016210783B2 (en) 2018-06-28
AU2010313386A1 (en) 2012-06-14
CN105456292B (zh) 2019-03-26
AR078849A1 (es) 2011-12-07
CA3169831A1 (en) 2011-05-05
JP2016127847A (ja) 2016-07-14

Similar Documents

Publication Publication Date Title
CL2012001117A1 (es) Composicion que comprende celulas madres multipotenciales del arbol biliar de un mamífero; método para obtener células multipotentes de un mamífero capaz de diferenciarse en multiples linajes endotérmicos.
EP2504426A4 (en) METHOD FOR PRODUCING MESENCHYMAL STEM CELLS AND COMPOSITIONS AND KIT THEREWITH
PH12012500818A1 (en) Pluripotent stem cells
PH12012501355A1 (en) Certain triazolopyridines and triazolopyrazines, compositions thereof and methods of use therefor
WO2010111409A3 (en) Pluripotent stem cells
BRPI0921799A2 (pt) compostos que expandem células-tronco hematopoéticas
ES2690212T3 (es) Células estromales mesenquimales y usos relacionados con las mismas
SG10201408475VA (en) Brassica plants yielding oils with a low total saturated fatty acid content
MX2009013706A (es) Celulas multipotentes/pluripotentes y metodos relacionados.
EP2373784A4 (en) GENERATION AND CONSERVATION OF STEM CELLS
WO2013177133A3 (en) Generation of human ips cells by a synthetic self- replicative rna
HK1222205A1 (zh) 由多能干细胞产生的光感受器和光感受器袓细胞
CL2012000590A1 (es) Proceso para preparar w-transaminasa (r)-selectiva.
CL2011002669A1 (es) Tabaco tratado para un articulo para fumar en que el tabaco porta diluyente y material de barrera en donde el diluyente se aplica al tabaco antes que o simultaneamente con el material de barrera, articulo para fumar; y metodo para producir el tabaco tratado.
CL2012003383A1 (es) Metodo para predecir un fenotipo en una planta de una poblacion sometida a predicción
BR112012012192A2 (pt) b-glicosidades variantes recombinantes thermoascus aurantiacus para produção de açúcares fermentáveis a partir da biomassa celulósica
IN2012DN00852A (enExample)
GT201300256A (es) Procesos para la elaboración de depsipéptidos macrocíclicos y nuevos intermediarios
MX349684B (es) Composiciones que contienen peliculas.
BR112013019822A2 (pt) método para remover água a partir de um material que possui água livre associada, produto, método para produzir estruvita substancialmente seca, e, composição para secagem química
WO2011032025A3 (en) Adipose-derived induced pluripotent stem cells
EA201290801A1 (ru) Способ получения сциллоинозита
CO6470817A2 (es) Cristales de solvato novedosos
IN2013MN00607A (enExample)
UY34544A (es) Determinación colorimétrica del contenido total de aceite de una muestra de planta mediante saponificación alcalina.