CA2779144C - Multipotent stem cells from the extrahepatic biliary tree and methods of isolating same - Google Patents

Multipotent stem cells from the extrahepatic biliary tree and methods of isolating same Download PDF

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Publication number
CA2779144C
CA2779144C CA2779144A CA2779144A CA2779144C CA 2779144 C CA2779144 C CA 2779144C CA 2779144 A CA2779144 A CA 2779144A CA 2779144 A CA2779144 A CA 2779144A CA 2779144 C CA2779144 C CA 2779144C
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Prior art keywords
cells
biliary tree
tissue
biliary
duct
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CA2779144A
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English (en)
French (fr)
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CA2779144A1 (en
Inventor
Lola M. Reid
Yunfang Wang
Vincenzo Cardinale
Eugenio Gaudio
Guido Carpino
Domenico Alvaro
Cai-Bin Cui
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Sapienza University of Rome
University of North Carolina at Chapel Hill
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Sapienza University of Rome
University of North Carolina at Chapel Hill
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Application filed by Sapienza University of Rome, University of North Carolina at Chapel Hill filed Critical Sapienza University of Rome
Priority to CA3169831A priority Critical patent/CA3169831C/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/413—Gall bladder; Bile
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/068—Stem cells; Progenitors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/38—Hormones with nuclear receptors
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CA2779144A 2009-10-30 2010-10-28 Multipotent stem cells from the extrahepatic biliary tree and methods of isolating same Active CA2779144C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3169831A CA3169831C (en) 2009-10-30 2010-10-28 Pluripodent Stem Cells from the Extrahepatic Biliary Tree and Methods for Their Isolation

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25684609P 2009-10-30 2009-10-30
US61/256,846 2009-10-30
PCT/US2010/054450 WO2011053690A1 (en) 2009-10-30 2010-10-28 Multipotent stem cells from the extrahepatic billary tree and methods of isolating same

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3169831A Division CA3169831C (en) 2009-10-30 2010-10-28 Pluripodent Stem Cells from the Extrahepatic Biliary Tree and Methods for Their Isolation

Publications (2)

Publication Number Publication Date
CA2779144A1 CA2779144A1 (en) 2011-05-05
CA2779144C true CA2779144C (en) 2022-10-25

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ID=43922533

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2779144A Active CA2779144C (en) 2009-10-30 2010-10-28 Multipotent stem cells from the extrahepatic biliary tree and methods of isolating same
CA3169831A Active CA3169831C (en) 2009-10-30 2010-10-28 Pluripodent Stem Cells from the Extrahepatic Biliary Tree and Methods for Their Isolation

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CA3169831A Active CA3169831C (en) 2009-10-30 2010-10-28 Pluripodent Stem Cells from the Extrahepatic Biliary Tree and Methods for Their Isolation

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US (1) US20110135610A1 (enExample)
EP (2) EP3725874A1 (enExample)
JP (2) JP5936547B2 (enExample)
KR (4) KR20200146043A (enExample)
CN (2) CN102918145B (enExample)
AR (2) AR078849A1 (enExample)
AU (1) AU2016210783B2 (enExample)
BR (1) BR112012009848B1 (enExample)
CA (2) CA2779144C (enExample)
CL (1) CL2012001117A1 (enExample)
CR (1) CR20120254A (enExample)
ES (1) ES2819028T3 (enExample)
IL (2) IL219454A (enExample)
MX (1) MX2012005087A (enExample)
NZ (3) NZ709124A (enExample)
PH (1) PH12012500845A1 (enExample)
RU (2) RU2756584C2 (enExample)
SG (2) SG10201913006XA (enExample)
TW (3) TWI641688B (enExample)
WO (1) WO2011053690A1 (enExample)

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WO2012178215A1 (en) 2011-06-23 2012-12-27 The Children's Hospital Of Philadelphia Self-renewing endodermal progenitor lines generated from human pluripotent stem cells and methods of use thereof
US9533013B2 (en) 2013-03-13 2017-01-03 University Of North Carolina At Chapel Hill Method of treating pancreatic and liver conditions by endoscopic-mediated (or laparoscopic-mediated) transplantation of stem cells into/onto bile duct walls of particular regions of the biliary tree
CN103266084B (zh) * 2013-05-27 2015-03-04 刘卫辉 富集干细胞的三步分离法
CN106461676B (zh) * 2014-03-17 2019-07-05 新加坡科技研究局 基于核因子κB易位预测化合物的毒性的方法
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US10683486B2 (en) * 2015-10-30 2020-06-16 Biolamina Ab Methods for producing hepatocytes
GB201521179D0 (en) 2015-12-01 2016-01-13 Univ Leuven Kath Methods for differentiating cells into hepatic stellate cells
CN105807053B (zh) * 2016-04-15 2019-04-02 苏州药明康德新药开发股份有限公司 一种肿瘤解离试剂在流式细胞检测中的应用
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
KR20190141168A (ko) * 2017-04-06 2019-12-23 더 유니버시티 오브 노쓰 캐롤라이나 엣 채플 힐 동결보존 방법
CA3112650A1 (en) * 2018-03-29 2019-10-03 The University Of North Carolina At Chapel Hill Stem/progenitor cells from duodenal brunner's glands and methods of isolating and using them
CN112126618B (zh) * 2019-06-24 2023-09-12 上海拜羡生物科技有限公司 一种人胆囊干细胞的获取和长期体外培养的方法
CN113388569B (zh) * 2020-08-28 2022-05-17 广东乾晖生物科技有限公司 肝脏类器官的制备方法
BR112023025612A2 (pt) * 2021-06-30 2024-02-27 Polbionica Sp Z O O Meio para armazenar ilhotas pancreáticas isoladas, e, método de armazenamento de ilhotas pancreáticas isoladas
CN116478904A (zh) * 2022-01-17 2023-07-25 香港中文大学 肝样细胞和人工肝组织的制备方法以及应用
CN115975913B (zh) * 2023-02-03 2024-06-04 北京中医药大学深圳医院(龙岗) 一种利用多能干细胞诱导分化胆囊祖细胞的方法

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