CH658728A5 - Process for the production of particulate reagents for immunoassays, and these reagents and their use - Google Patents
Process for the production of particulate reagents for immunoassays, and these reagents and their use Download PDFInfo
- Publication number
- CH658728A5 CH658728A5 CH1367/79A CH136779A CH658728A5 CH 658728 A5 CH658728 A5 CH 658728A5 CH 1367/79 A CH1367/79 A CH 1367/79A CH 136779 A CH136779 A CH 136779A CH 658728 A5 CH658728 A5 CH 658728A5
- Authority
- CH
- Switzerland
- Prior art keywords
- reagent
- particles
- particulate
- monomer
- polymer matrix
- Prior art date
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 84
- 238000000034 method Methods 0.000 title claims description 24
- 238000003018 immunoassay Methods 0.000 title claims description 10
- 238000004519 manufacturing process Methods 0.000 title description 8
- 239000002245 particle Substances 0.000 claims description 80
- 239000011159 matrix material Substances 0.000 claims description 24
- 239000000725 suspension Substances 0.000 claims description 24
- 239000000178 monomer Substances 0.000 claims description 20
- 229920000642 polymer Polymers 0.000 claims description 17
- HGINCPLSRVDWNT-UHFFFAOYSA-N Acrolein Chemical compound C=CC=O HGINCPLSRVDWNT-UHFFFAOYSA-N 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 13
- 239000011541 reaction mixture Substances 0.000 claims description 11
- 239000007790 solid phase Substances 0.000 claims description 11
- 238000006116 polymerization reaction Methods 0.000 claims description 10
- KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 claims description 10
- 230000008878 coupling Effects 0.000 claims description 8
- 238000010168 coupling process Methods 0.000 claims description 8
- 238000005859 coupling reaction Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 claims description 6
- 239000000427 antigen Substances 0.000 claims description 6
- 102000036639 antigens Human genes 0.000 claims description 6
- 108091007433 antigens Proteins 0.000 claims description 6
- 239000007791 liquid phase Substances 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 238000004458 analytical method Methods 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 239000003054 catalyst Substances 0.000 claims description 4
- 239000003999 initiator Substances 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- GYCMBHHDWRMZGG-UHFFFAOYSA-N Methylacrylonitrile Chemical compound CC(=C)C#N GYCMBHHDWRMZGG-UHFFFAOYSA-N 0.000 claims description 3
- HFBMWMNUJJDEQZ-UHFFFAOYSA-N acryloyl chloride Chemical compound ClC(=O)C=C HFBMWMNUJJDEQZ-UHFFFAOYSA-N 0.000 claims description 3
- 125000003172 aldehyde group Chemical group 0.000 claims description 3
- 150000001299 aldehydes Chemical class 0.000 claims description 3
- VHRYZQNGTZXDNX-UHFFFAOYSA-N methacryloyl chloride Chemical compound CC(=C)C(Cl)=O VHRYZQNGTZXDNX-UHFFFAOYSA-N 0.000 claims description 3
- 239000011236 particulate material Substances 0.000 claims description 3
- JIHQDMXYYFUGFV-UHFFFAOYSA-N 1,3,5-triazine Chemical compound C1=NC=NC=N1 JIHQDMXYYFUGFV-UHFFFAOYSA-N 0.000 claims description 2
- NOWKCMXCCJGMRR-UHFFFAOYSA-N Aziridine Chemical compound C1CN1 NOWKCMXCCJGMRR-UHFFFAOYSA-N 0.000 claims description 2
- 239000004593 Epoxy Substances 0.000 claims description 2
- ZMZDMBWJUHKJPS-UHFFFAOYSA-M Thiocyanate anion Chemical compound [S-]C#N ZMZDMBWJUHKJPS-UHFFFAOYSA-M 0.000 claims description 2
- 125000004018 acid anhydride group Chemical group 0.000 claims description 2
- 125000003118 aryl group Chemical group 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 2
- SXZHPAWDTKNSMJ-UHFFFAOYSA-N cyanato peroxycyanate Chemical compound N#COOOC#N SXZHPAWDTKNSMJ-UHFFFAOYSA-N 0.000 claims description 2
- 125000003700 epoxy group Chemical group 0.000 claims description 2
- 125000000524 functional group Chemical group 0.000 claims description 2
- 125000005842 heteroatom Chemical group 0.000 claims description 2
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 claims description 2
- 150000003949 imides Chemical class 0.000 claims description 2
- 125000001841 imino group Chemical group [H]N=* 0.000 claims description 2
- 150000002540 isothiocyanates Chemical class 0.000 claims description 2
- CTAPFRYPJLPFDF-UHFFFAOYSA-N isoxazole Chemical compound C=1C=NOC=1 CTAPFRYPJLPFDF-UHFFFAOYSA-N 0.000 claims description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- YBBRCQOCSYXUOC-UHFFFAOYSA-N sulfuryl dichloride Chemical compound ClS(Cl)(=O)=O YBBRCQOCSYXUOC-UHFFFAOYSA-N 0.000 claims description 2
- 125000003396 thiol group Chemical group [H]S* 0.000 claims description 2
- 229920003176 water-insoluble polymer Polymers 0.000 claims description 2
- 239000000470 constituent Substances 0.000 claims 2
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 claims 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims 1
- VDOKWPVSGXHSNP-UHFFFAOYSA-N 2-methylprop-1-en-1-one Chemical compound CC(C)=C=O VDOKWPVSGXHSNP-UHFFFAOYSA-N 0.000 claims 1
- OWIKHYCFFJSOEH-UHFFFAOYSA-N Isocyanic acid Chemical compound N=C=O OWIKHYCFFJSOEH-UHFFFAOYSA-N 0.000 claims 1
- STNJBCKSHOAVAJ-UHFFFAOYSA-N Methacrolein Chemical compound CC(=C)C=O STNJBCKSHOAVAJ-UHFFFAOYSA-N 0.000 claims 1
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 claims 1
- 210000002966 serum Anatomy 0.000 description 16
- 239000000243 solution Substances 0.000 description 15
- 238000010790 dilution Methods 0.000 description 13
- 239000012895 dilution Substances 0.000 description 13
- 238000003556 assay Methods 0.000 description 9
- 238000009739 binding Methods 0.000 description 9
- 230000027455 binding Effects 0.000 description 8
- 239000003085 diluting agent Substances 0.000 description 6
- 229940088597 hormone Drugs 0.000 description 6
- 239000005556 hormone Substances 0.000 description 6
- 239000008363 phosphate buffer Substances 0.000 description 6
- 210000002826 placenta Anatomy 0.000 description 6
- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 description 5
- 229960005156 digoxin Drugs 0.000 description 5
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 description 5
- SZVJSHCCFOBDDC-UHFFFAOYSA-N ferrosoferric oxide Chemical compound O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 238000003127 radioimmunoassay Methods 0.000 description 5
- 229910000859 α-Fe Inorganic materials 0.000 description 5
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 230000001983 lactogenic effect Effects 0.000 description 4
- 230000009871 nonspecific binding Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 102000014914 Carrier Proteins Human genes 0.000 description 3
- 108091008324 binding proteins Proteins 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000012265 solid product Substances 0.000 description 3
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 229910001870 ammonium persulfate Inorganic materials 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 239000013067 intermediate product Substances 0.000 description 2
- 239000006249 magnetic particle Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000005405 multipole Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 102100021809 Chorionic somatomammotropin hormone 1 Human genes 0.000 description 1
- XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 description 1
- HCYAFALTSJYZDH-UHFFFAOYSA-N Desimpramine Chemical compound C1CC2=CC=CC=C2N(CCCNC)C2=CC=CC=C21 HCYAFALTSJYZDH-UHFFFAOYSA-N 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 101000895818 Homo sapiens Chorionic somatomammotropin hormone 1 Proteins 0.000 description 1
- 101000956228 Homo sapiens Chorionic somatomammotropin hormone 2 Proteins 0.000 description 1
- PHVGLTMQBUFIQQ-UHFFFAOYSA-N Nortryptiline Chemical compound C1CC2=CC=CC=C2C(=CCCNC)C2=CC=CC=C21 PHVGLTMQBUFIQQ-UHFFFAOYSA-N 0.000 description 1
- URWAJWIAIPFPJE-UHFFFAOYSA-N Rickamicin Natural products O1CC(O)(C)C(NC)C(O)C1OC1C(O)C(OC2C(CC=C(CN)O2)N)C(N)CC1N URWAJWIAIPFPJE-UHFFFAOYSA-N 0.000 description 1
- 229940123445 Tricyclic antidepressant Drugs 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 229960004821 amikacin Drugs 0.000 description 1
- LKCWBDHBTVXHDL-RMDFUYIESA-N amikacin Chemical compound O([C@@H]1[C@@H](N)C[C@H]([C@@H]([C@H]1O)O[C@@H]1[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O1)O)NC(=O)[C@@H](O)CCN)[C@H]1O[C@H](CN)[C@@H](O)[C@H](O)[C@H]1O LKCWBDHBTVXHDL-RMDFUYIESA-N 0.000 description 1
- 239000002647 aminoglycoside antibiotic agent Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 238000012662 bulk polymerization Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 230000009137 competitive binding Effects 0.000 description 1
- 238000005112 continuous flow technique Methods 0.000 description 1
- 229960003914 desipramine Drugs 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- -1 haptens Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- IQPQWNKOIGAROB-UHFFFAOYSA-N isocyanate Chemical compound [N-]=C=O IQPQWNKOIGAROB-UHFFFAOYSA-N 0.000 description 1
- 239000012948 isocyanate Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 125000000468 ketone group Chemical group 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 229960000808 netilmicin Drugs 0.000 description 1
- ZBGPYVZLYBDXKO-HILBYHGXSA-N netilmycin Chemical compound O([C@@H]1[C@@H](N)C[C@H]([C@@H]([C@H]1O)O[C@@H]1[C@]([C@H](NC)[C@@H](O)CO1)(C)O)NCC)[C@H]1OC(CN)=CC[C@H]1N ZBGPYVZLYBDXKO-HILBYHGXSA-N 0.000 description 1
- 229960001158 nortriptyline Drugs 0.000 description 1
- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 238000010526 radical polymerization reaction Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- URWAJWIAIPFPJE-YFMIWBNJSA-N sisomycin Chemical compound O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H](CC=C(CN)O2)N)[C@@H](N)C[C@H]1N URWAJWIAIPFPJE-YFMIWBNJSA-N 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
- 239000005495 thyroid hormone Substances 0.000 description 1
- 229940036555 thyroid hormone Drugs 0.000 description 1
- 229940034208 thyroxine Drugs 0.000 description 1
- XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 description 1
- 239000003029 tricyclic antidepressant agent Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/5434—Magnetic particles using magnetic particle immunoreagent carriers which constitute new materials per se
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2446/00—Magnetic particle immunoreagent carriers
- G01N2446/20—Magnetic particle immunoreagent carriers the magnetic material being present in the particle core
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2446/00—Magnetic particle immunoreagent carriers
- G01N2446/40—Magnetic particle immunoreagent carriers the magnetic material being dispersed in the monomer composition prior to polymerisation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2446/00—Magnetic particle immunoreagent carriers
- G01N2446/80—Magnetic particle immunoreagent carriers characterised by the agent used to coat the magnetic particles, e.g. lipids
- G01N2446/86—Magnetic particle immunoreagent carriers characterised by the agent used to coat the magnetic particles, e.g. lipids the coating being pre-functionalised for attaching immunoreagents, e.g. aminodextran
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2446/00—Magnetic particle immunoreagent carriers
- G01N2446/80—Magnetic particle immunoreagent carriers characterised by the agent used to coat the magnetic particles, e.g. lipids
- G01N2446/90—Magnetic particle immunoreagent carriers characterised by the agent used to coat the magnetic particles, e.g. lipids characterised by small molecule linker used to couple immunoreagents to magnetic particles
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Compositions Of Macromolecular Compounds (AREA)
- Polymerisation Methods In General (AREA)
- Graft Or Block Polymers (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB567178 | 1978-02-13 |
Publications (1)
Publication Number | Publication Date |
---|---|
CH658728A5 true CH658728A5 (en) | 1986-11-28 |
Family
ID=9800467
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CH1367/79A CH658728A5 (en) | 1978-02-13 | 1979-02-13 | Process for the production of particulate reagents for immunoassays, and these reagents and their use |
Country Status (10)
Country | Link |
---|---|
JP (2) | JPS54145211A (fr) |
AU (1) | AU527144B2 (fr) |
BE (1) | BE874091A (fr) |
CA (1) | CA1136400A (fr) |
CH (1) | CH658728A5 (fr) |
DE (1) | DE2905154A1 (fr) |
FR (1) | FR2417106A1 (fr) |
IT (1) | IT1119273B (fr) |
NL (1) | NL7901025A (fr) |
SE (1) | SE7901166L (fr) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3582649D1 (de) * | 1984-11-01 | 1991-05-29 | Technicon Instr | Magnetisch empfindlicher reagenstraeger und verfahren zur herstellung. |
JPH073425B2 (ja) * | 1985-01-19 | 1995-01-18 | 東ソー株式会社 | 免疫反応用担体の製造方法 |
JPH07113640B2 (ja) * | 1986-07-07 | 1995-12-06 | 東ソー株式会社 | 蛋白質固定化用担体およびその製法 |
JP2672151B2 (ja) * | 1989-07-14 | 1997-11-05 | 三井東圧化学株式会社 | 磁性体を利用した酵素免疫測定法 |
JPH03109648U (fr) * | 1990-02-27 | 1991-11-11 | ||
DE10235302A1 (de) * | 2002-08-01 | 2004-02-12 | Henkel Kgaa | Verfahren zur Herstellung perl- bzw. kugelförmiger magnetischer Partikel auf Acrylsäuresbasis zur Aufreinigung biologischer Substanzen |
JP4812105B2 (ja) * | 2006-11-10 | 2011-11-09 | ピジョン株式会社 | 食事用エプロン |
CN101801181A (zh) * | 2007-07-19 | 2010-08-11 | 凯米克有限公司 | 生物杀灭性聚丙烯醛组合物 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3296234A (en) * | 1964-06-23 | 1967-01-03 | Grace W R & Co | Aqueous process for polymerizing acrylonitrile in the presence of silver nitrate and a hydrocarbon liquid |
JPS5146552B2 (fr) * | 1972-12-04 | 1976-12-09 | ||
FR2313394A1 (fr) * | 1975-06-06 | 1976-12-31 | Nestle Sa | Procede de preparation d'un produit doue d'activite enzymatique |
FR2334106A1 (fr) * | 1975-12-02 | 1977-07-01 | Pasteur Institut | Gel magnetique convenant pour dosages immunoenzymatiques |
GB1575805A (en) * | 1976-03-12 | 1980-10-01 | Technicon Instr | Automatic diagnostic apparatus |
-
1979
- 1979-02-09 NL NL7901025A patent/NL7901025A/xx not_active Application Discontinuation
- 1979-02-09 AU AU44115/79A patent/AU527144B2/en not_active Ceased
- 1979-02-10 DE DE19792905154 patent/DE2905154A1/de active Granted
- 1979-02-12 BE BE0/193397A patent/BE874091A/fr not_active IP Right Cessation
- 1979-02-12 IT IT67300/79A patent/IT1119273B/it active
- 1979-02-12 FR FR7903485A patent/FR2417106A1/fr active Granted
- 1979-02-12 CA CA000321254A patent/CA1136400A/fr not_active Expired
- 1979-02-13 CH CH1367/79A patent/CH658728A5/de not_active IP Right Cessation
- 1979-02-13 JP JP1439579A patent/JPS54145211A/ja active Granted
- 1979-05-07 SE SE7901166A patent/SE7901166L/ not_active Application Discontinuation
-
1987
- 1987-09-09 JP JP62224192A patent/JPS63183909A/ja active Granted
Also Published As
Publication number | Publication date |
---|---|
BE874091A (fr) | 1979-08-13 |
FR2417106B1 (fr) | 1984-08-10 |
DE2905154A1 (de) | 1979-08-30 |
FR2417106A1 (fr) | 1979-09-07 |
SE7901166L (sv) | 1979-08-14 |
JPH0459587B2 (fr) | 1992-09-22 |
JPS6342229B2 (fr) | 1988-08-22 |
IT1119273B (it) | 1986-03-10 |
JPS63183909A (ja) | 1988-07-29 |
DE2905154C2 (fr) | 1989-09-14 |
NL7901025A (nl) | 1979-08-15 |
CA1136400A (fr) | 1982-11-30 |
AU4411579A (en) | 1979-10-25 |
AU527144B2 (en) | 1983-02-17 |
JPS54145211A (en) | 1979-11-13 |
IT7967300A0 (it) | 1979-02-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE2522086C2 (de) | Reagenz zum Abtrennen und Bestimmen von in biologischen Fluiden vorhandenen AK:Ag-Komplexen | |
DE69121849T2 (de) | Bindung von Liganden an Gold | |
DE69021309T2 (de) | Magnetische Teilchen verwendender Immunotest. | |
DE2548427C2 (de) | Einrichtung zur Verwendung bei der Festphasen-Immunanalyse einer Komponente einer Antigen-Antikörper-Reaktion und Verfahren zu ihrer Herstellung | |
EP1419386B1 (fr) | Procede de production de microparticules chargees de proteines | |
EP0019741B1 (fr) | Réactif de latex, procédé pour sa préparation, son utilisation et préparation contenant ce réactif | |
DE2943648C2 (de) | Spezifische Bindungs-Untersuchungsmethode zur Bestimmung einer Vielzahl von verschiedenen Liganden in einer einzigen Flüssigkeitsprobe | |
DE68911674T2 (de) | Testverfahren und reagenzsatz dazu. | |
DE3689618T2 (de) | Magnetische polymerteilchen. | |
CH639208A5 (de) | Immunoassays unter verwendung von f(ab')(2)-untergruppen. | |
DE2902677A1 (de) | Unloeslich gemachte proteine und immunoassays unter verwendung dieser proteine | |
DE69106002T2 (de) | Testverfahren und reagenziensatz dafür. | |
EP0253254B1 (fr) | Polymères en dispersion, polymères biologiquement actifs en dispersion, leur procédé de préparation et leur utilisation comme moyen de diagnostic | |
CH658728A5 (en) | Process for the production of particulate reagents for immunoassays, and these reagents and their use | |
DE69428445T2 (de) | Magnetische mit gelatine überzogene teilchen und sie verwendender immunoassay | |
DE69527877T2 (de) | Mikropartikel-Immunoassayreagentien, empfindliche und spezifische Immunoassayreagentien und Immunoassaymethode | |
EP0215457A2 (fr) | Procédé pour la détermination d'un co-réactant d'une réaction immunologique | |
EP0537488B1 (fr) | Sérums de contrÔle et standard artificiels pour utilisation dans des essais de determination d'anticorps | |
DE2912239C2 (de) | Verfahren zur Herstellung eines Festphasenträgers für die Radioimmunoanalyse sowie dessen Verwendung in chromatographischen Säulen | |
DE69022795T2 (de) | Nachweis eines Antikörpers und dessen Immunglobulin-Klasse. | |
DE68918286T2 (de) | Verfahren zum testen einer immunologisch aktiven substanz und dafür geeignetes reagenz. | |
DE2841782C2 (fr) | ||
EP0055869A2 (fr) | Procédé pour la détermination de ligands, trousse d'essai pour la réalisation dudit procédé et son application | |
EP0290017B1 (fr) | Procédé pour déterminer quantitativement les protéines de sérum dans les liquides du corps et composition pour sa réalisation | |
DE69216356T2 (de) | Diagnosetestsatz und spezifischer Bindungstest unter Verwendung eines Peroxidase Markierungsmittels und eines Signalmodulators |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PL | Patent ceased |