CA3007635A1 - Promoteurs de corynebacterium glutamicum - Google Patents
Promoteurs de corynebacterium glutamicum Download PDFInfo
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- CA3007635A1 CA3007635A1 CA3007635A CA3007635A CA3007635A1 CA 3007635 A1 CA3007635 A1 CA 3007635A1 CA 3007635 A CA3007635 A CA 3007635A CA 3007635 A CA3007635 A CA 3007635A CA 3007635 A1 CA3007635 A1 CA 3007635A1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
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- Life Sciences & Earth Sciences (AREA)
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- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La présente invention concerne des promoteurs natifs comprenant des polynucléotides isolés à partir de Corynebacterium glutamicum, ainsi que des promoteurs mutants dérivés de ceux-ci, qui peuvent être utilisés pour réguler, c'est-à-dire, augmenter ou réduire, l'expression génique. L'invention concerne également des échelles de promoteurs comprenant une pluralité des promoteurs présentant une activité de promoteur augmentant progressivement. L'invention concerne également des cellules hôtes et des vecteurs recombinés comprenant lesdits promoteurs, ainsi que des procédés d'expression de gènes d'intérêt et de production de biomolécules au moyen des cellules hôtes.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562264232P | 2015-12-07 | 2015-12-07 | |
| US62/264,232 | 2015-12-07 | ||
| US201662431409P | 2016-12-07 | 2016-12-07 | |
| PCT/US2016/065464 WO2017100376A2 (fr) | 2015-12-07 | 2016-12-07 | Promoteurs de corynebacterium glutamicum |
| US62/431,409 | 2016-12-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3007635A1 true CA3007635A1 (fr) | 2017-06-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3007635A Abandoned CA3007635A1 (fr) | 2015-12-07 | 2016-12-07 | Promoteurs de corynebacterium glutamicum |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US11293029B2 (fr) |
| JP (1) | JP6821598B2 (fr) |
| KR (1) | KR20180084756A (fr) |
| BR (1) | BR112018011503A2 (fr) |
| CA (1) | CA3007635A1 (fr) |
| WO (1) | WO2017100376A2 (fr) |
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| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
| CA3007635A1 (fr) | 2015-12-07 | 2017-06-15 | Zymergen Inc. | Promoteurs de corynebacterium glutamicum |
| KR102345898B1 (ko) | 2016-06-30 | 2022-01-03 | 지머젠 인코포레이티드 | 글루코오스 투과 효소 라이브러리를 생성하는 방법 및 이의 용도 |
| US10544390B2 (en) | 2016-06-30 | 2020-01-28 | Zymergen Inc. | Methods for generating a bacterial hemoglobin library and uses thereof |
| JP2019519243A (ja) | 2016-07-05 | 2019-07-11 | ザイマージェン インコーポレイテッド | Ｒｎａデグラドソームタンパク質複合体の遺伝的攪乱 |
| EP3625351A1 (fr) | 2017-05-19 | 2020-03-25 | Zymergen Inc. | Ingénierie génomique des voies de biosynthèse conduisant à un accroissement du nadph |
| CA3064607A1 (fr) | 2017-06-06 | 2018-12-13 | Zymergen Inc. | Mutagenese de transposon a haut debit |
| JP7350659B2 (ja) | 2017-06-06 | 2023-09-26 | ザイマージェン インコーポレイテッド | Ｓａｃｃｈａｒｏｐｏｌｙｓｐｏｒａ ｓｐｉｎｏｓａの改良のためのハイスループット（ＨＴＰ）ゲノム操作プラットフォーム |
| US20200370058A1 (en) * | 2017-06-06 | 2020-11-26 | Zymergen Inc. | A htp genomic engineering platform for improving escherichia coli |
| CA3064053A1 (fr) | 2017-06-06 | 2018-12-13 | Zymergen Inc. | Hierarchisation de modifications genetiques pour augmenter le rendement de l'optimisation phenotypique |
| CA3061984A1 (fr) | 2017-06-06 | 2018-12-13 | Zymergen Inc. | Plate-forme d'ingenierie genomique htp permettant d'ameliorer les souches fongiques |
| WO2018226964A2 (fr) * | 2017-06-07 | 2018-12-13 | Zymergen Inc. | Promoteurs issus de corynebacterium glutamicum et leurs utilisations dans la régulation de l'expression génique ancillaire |
| CN108359669B (zh) * | 2018-02-13 | 2020-04-07 | 江南大学 | 一种棒杆菌启动子及其应用 |
| EP3768844A4 (fr) | 2018-03-20 | 2021-12-15 | Zymergen Inc. | Plateforme htp pour ingénierie génétique de cellules d'ovaire de hamster chinois |
| CA3097071A1 (fr) | 2018-06-06 | 2019-12-12 | Zymergen Inc. | Manipulation de genes impliques dans la transduction de signal pour reguler la morphologie fongique pendant la fermentation et la production |
| EP3847270A1 (fr) * | 2018-09-07 | 2021-07-14 | Archer Daniels Midland Company | Souches génétiquement modifiées de corynebactéries |
| US11053515B2 (en) | 2019-03-08 | 2021-07-06 | Zymergen Inc. | Pooled genome editing in microbes |
| CN113728106A (zh) | 2019-03-08 | 2021-11-30 | 齐默尔根公司 | 微生物中的迭代基因组编辑 |
| US11479779B2 (en) | 2020-07-31 | 2022-10-25 | Zymergen Inc. | Systems and methods for high-throughput automated strain generation for non-sporulating fungi |
| AU2021432764A1 (en) * | 2021-03-09 | 2023-09-28 | Cj Cheiljedang Corporation | Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine by using same |
| CN112695036B (zh) * | 2021-03-23 | 2021-07-06 | 中国科学院天津工业生物技术研究所 | 一种天冬氨酸激酶基因表达调控序列及其应用 |
| CN115449519B (zh) * | 2021-06-08 | 2023-04-07 | 中国科学院天津工业生物技术研究所 | 基于dapB基因的具有启动子活性的多核苷酸及其用途 |
| WO2022265130A1 (fr) * | 2021-06-15 | 2022-12-22 | 씨제이제일제당 (주) | Nouveau promoteur et son utilisation |
| CN116536310A (zh) * | 2022-01-26 | 2023-08-04 | 廊坊梅花生物技术开发有限公司 | 启动子、产苏氨酸重组微生物及其应用 |
| CN114835783A (zh) * | 2022-06-01 | 2022-08-02 | 宁夏伊品生物科技股份有限公司 | NCgl2747基因突变体及其在制备L-赖氨酸中的应用 |
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- 2016-12-07 KR KR1020187011781A patent/KR20180084756A/ko unknown
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|---|---|
| WO2017100376A2 (fr) | 2017-06-15 |
| JP6821598B2 (ja) | 2021-01-27 |
| US20180362991A1 (en) | 2018-12-20 |
| WO2017100376A9 (fr) | 2017-12-21 |
| WO2017100376A3 (fr) | 2017-08-03 |
| US20220325291A1 (en) | 2022-10-13 |
| KR20180084756A (ko) | 2018-07-25 |
| JP2018530991A (ja) | 2018-10-25 |
| US11293029B2 (en) | 2022-04-05 |
| BR112018011503A2 (pt) | 2018-12-04 |
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