LT3474669T - Barkodu pažymėtų kombinatorinių bibliotekų generavimo būdai - Google Patents
Barkodu pažymėtų kombinatorinių bibliotekų generavimo būdaiInfo
- Publication number
- LT3474669T LT3474669T LTEPPCT/US2017/039146T LTUS2017039146T LT3474669T LT 3474669 T LT3474669 T LT 3474669T LT US2017039146 T LTUS2017039146 T LT US2017039146T LT 3474669 T LT3474669 T LT 3474669T
- Authority
- LT
- Lithuania
- Prior art keywords
- methods
- combinatorial libraries
- generating barcoded
- barcoded combinatorial
- generating
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662354516P | 2016-06-24 | 2016-06-24 | |
US201662367386P | 2016-07-27 | 2016-07-27 | |
US201762483930P | 2017-04-10 | 2017-04-10 | |
PCT/US2017/039146 WO2017223538A1 (en) | 2016-06-24 | 2017-06-23 | Methods for generating barcoded combinatorial libraries |
Publications (1)
Publication Number | Publication Date |
---|---|
LT3474669T true LT3474669T (lt) | 2022-06-10 |
Family
ID=60676029
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
LTEPPCT/US2017/039146T LT3474669T (lt) | 2016-06-24 | 2017-06-23 | Barkodu pažymėtų kombinatorinių bibliotekų generavimo būdai |
Country Status (10)
Country | Link |
---|---|
US (5) | US10017760B2 (lt) |
EP (1) | EP3474669B1 (lt) |
JP (1) | JP2019518478A (lt) |
CN (1) | CN109688820B (lt) |
AU (1) | AU2017280353B2 (lt) |
CA (1) | CA3029254A1 (lt) |
DK (1) | DK3474669T3 (lt) |
ES (1) | ES2915562T3 (lt) |
LT (1) | LT3474669T (lt) |
WO (1) | WO2017223538A1 (lt) |
Families Citing this family (95)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP4063503A1 (en) | 2014-02-11 | 2022-09-28 | The Regents of the University of Colorado, a body corporate | Crispr enabled multiplexed genome engineering |
WO2017100376A2 (en) | 2015-12-07 | 2017-06-15 | Zymergen, Inc. | Promoters from corynebacterium glutamicum |
US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
EP4273248A3 (en) * | 2016-05-20 | 2024-01-10 | Braingene AB | Destabilising domains for conditionally stabilising a protein |
US10337051B2 (en) | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
US11293021B1 (en) | 2016-06-23 | 2022-04-05 | Inscripta, Inc. | Automated cell processing methods, modules, instruments, and systems |
LT3474669T (lt) | 2016-06-24 | 2022-06-10 | The Regents Of The University Of Colorado, A Body Corporate | Barkodu pažymėtų kombinatorinių bibliotekų generavimo būdai |
US10544411B2 (en) | 2016-06-30 | 2020-01-28 | Zymergen Inc. | Methods for generating a glucose permease library and uses thereof |
EP3478833A4 (en) | 2016-06-30 | 2019-10-02 | Zymergen, Inc. | METHODS OF GENERATING A BACTERIAL HEMOGLOBIN LIBRARY AND USES THEREOF |
US10892034B2 (en) | 2016-07-01 | 2021-01-12 | Microsoft Technology Licensing, Llc | Use of homology direct repair to record timing of a molecular event |
US20180004537A1 (en) * | 2016-07-01 | 2018-01-04 | Microsoft Technology Licensing, Llc | Molecular State Machines |
US11359234B2 (en) | 2016-07-01 | 2022-06-14 | Microsoft Technology Licensing, Llc | Barcoding sequences for identification of gene expression |
EP3478852B1 (en) | 2016-07-01 | 2020-08-12 | Microsoft Technology Licensing, LLC | Storage through iterative dna editing |
DK3500668T3 (da) * | 2016-08-17 | 2020-08-17 | Univ Leuven Kath | Identificering af mål for lægemiddel ved hurtig udvælgelse af lægemiddelresistente mutationer |
WO2018064352A1 (en) * | 2016-09-30 | 2018-04-05 | The Regents Of The University Of California | Rna-guided nucleic acid modifying enzymes and methods of use thereof |
GB2569733B (en) | 2016-09-30 | 2022-09-14 | Univ California | RNA-guided nucleic acid modifying enzymes and methods of use thereof |
RU2769475C2 (ru) * | 2017-06-23 | 2022-04-01 | Инскрипта, Инк. | Направляемые нуклеиновыми кислотами нуклеазы |
US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
DK3645719T3 (da) | 2017-06-30 | 2022-05-16 | Inscripta Inc | Automatiserede cellebehandlingsfremgangsmåder, moduler, instrumenter og systemer |
US10738327B2 (en) | 2017-08-28 | 2020-08-11 | Inscripta, Inc. | Electroporation cuvettes for automation |
CA3075532A1 (en) * | 2017-09-15 | 2019-03-21 | The Board Of Trustees Of The Leland Stanford Junior University | Multiplex production and barcoding of genetically engineered cells |
CN111372650A (zh) | 2017-09-30 | 2020-07-03 | 因思科瑞普特公司 | 流通式电穿孔仪器 |
SG11202003863VA (en) | 2017-11-01 | 2020-05-28 | Univ California | Casz compositions and methods of use |
WO2019089808A1 (en) | 2017-11-01 | 2019-05-09 | The Regents Of The University Of California | Class 2 crispr/cas compositions and methods of use |
WO2019152932A1 (en) * | 2018-02-05 | 2019-08-08 | The Regents Of The University Of Colorado, A Body Corporate | Construction and methods of use of a barcoded and gene edited dna library |
EP3752607A1 (en) * | 2018-02-15 | 2020-12-23 | Sigma Aldrich Co. LLC | Engineered cas9 systems for eukaryotic genome modification |
CN112204131A (zh) | 2018-03-29 | 2021-01-08 | 因思科瑞普特公司 | 用于诱导和转化的细胞生长速率的自动化控制 |
WO2019195701A1 (en) * | 2018-04-05 | 2019-10-10 | Massachusetts Eye And Ear Infirmary | Methods of making and using combinatorial barcoded nucleic acid libraries having defined variation |
WO2019200004A1 (en) | 2018-04-13 | 2019-10-17 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
US10557216B2 (en) | 2018-04-24 | 2020-02-11 | Inscripta, Inc. | Automated instrumentation for production of T-cell receptor peptide libraries |
US10508273B2 (en) | 2018-04-24 | 2019-12-17 | Inscripta, Inc. | Methods for identifying selective binding pairs |
US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
EP3813974A4 (en) | 2018-06-30 | 2022-08-03 | Inscripta, Inc. | INSTRUMENTS, MODULES AND METHODS FOR ENHANCED DETECTION OF EDITED SEQUENCES IN LIVING CELLS |
WO2020011985A1 (en) * | 2018-07-12 | 2020-01-16 | Keygene N.V. | Type v crispr/nuclease-system for genome editing in plant cells |
WO2020028729A1 (en) | 2018-08-01 | 2020-02-06 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
US10752874B2 (en) | 2018-08-14 | 2020-08-25 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
EP3841205A4 (en) * | 2018-08-22 | 2022-08-17 | The Regents of The University of California | VARIANT TYPE CRISPR/CAS V EFFECTIVE POLYPEPTIDES AND METHODS OF USE THEREOF |
US11718847B2 (en) * | 2018-08-29 | 2023-08-08 | Agilent Technologies, Inc. | Amplifying oligonucleotides and producing libraries of dual guide constructs |
CN112955540A (zh) | 2018-08-30 | 2021-06-11 | 因思科瑞普特公司 | 在自动化模块和仪器中对经核酸酶经编辑的序列的改进的检测 |
EP3861112A4 (en) * | 2018-10-04 | 2022-09-21 | The Regents of the University of Colorado, A Body Corporate | MODIFIED CHIMERIC NUCLEIC ACID GUIDED NUCLEASE CONSTRUCTS AND THEIR USES |
US11214781B2 (en) | 2018-10-22 | 2022-01-04 | Inscripta, Inc. | Engineered enzyme |
CN113227368B (zh) | 2018-10-22 | 2023-07-07 | 因思科瑞普特公司 | 工程化酶 |
WO2020092763A1 (en) * | 2018-11-03 | 2020-05-07 | Blueallele, Llc | Methods for comparing efficacy of donor molecules |
EP3931313A2 (en) | 2019-01-04 | 2022-01-05 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
WO2020163779A1 (en) * | 2019-02-08 | 2020-08-13 | The Board Of Trustees Of The Leland Stanford Junior University | Production and tracking of engineered cells with combinatorial genetic modifications |
CN113631713A (zh) | 2019-03-25 | 2021-11-09 | 因思科瑞普特公司 | 酵母中的同时多重基因组编辑 |
US11001831B2 (en) | 2019-03-25 | 2021-05-11 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
AU2020262429A1 (en) * | 2019-04-24 | 2021-12-16 | Spotlight Therapeutics | Methods and compositions for nucleic acid-guided nuclease cell targeting screen |
CA3139122C (en) | 2019-06-06 | 2023-04-25 | Inscripta, Inc. | Curing for recursive nucleic acid-guided cell editing |
TW202113074A (zh) | 2019-06-07 | 2021-04-01 | 美商斯奎柏治療公司 | 工程化之casx系統 |
EP3983536A4 (en) * | 2019-06-14 | 2023-11-29 | Arbor Biotechnologies, Inc. | NOVEL CRISPR DNA TARGETING ENZYMES AND SYSTEMS |
US10907125B2 (en) | 2019-06-20 | 2021-02-02 | Inscripta, Inc. | Flow through electroporation modules and instrumentation |
CA3139124C (en) | 2019-06-21 | 2023-01-31 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli |
US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
EP4028522A1 (en) | 2019-09-09 | 2022-07-20 | Scribe Therapeutics Inc. | Compositions and methods for the targeting of sod1 |
CN114729368A (zh) * | 2019-09-09 | 2022-07-08 | 斯克里贝治疗公司 | 用于免疫疗法的组合物和方法 |
CA3151872A1 (en) * | 2019-09-12 | 2021-03-18 | Glaxosmithkline Intellectual Property Development Limited | Method for screening libraries |
US20220315907A1 (en) * | 2019-10-10 | 2022-10-06 | Inscripta, Inc. | Split crispr nuclease tethering system |
EP4063500A4 (en) * | 2019-11-18 | 2023-12-27 | Suzhou Qi Biodesign biotechnology Company Limited | GENE EDITING SYSTEM DERIVED FROM BACTERIA OF THE GENUS FLAVOBACTERIUM |
WO2021102059A1 (en) | 2019-11-19 | 2021-05-27 | Inscripta, Inc. | Methods for increasing observed editing in bacteria |
WO2021113763A1 (en) | 2019-12-06 | 2021-06-10 | Scribe Therapeutics Inc. | Compositions and methods for the targeting of rhodopsin |
WO2021118626A1 (en) | 2019-12-10 | 2021-06-17 | Inscripta, Inc. | Novel mad nucleases |
US10704033B1 (en) | 2019-12-13 | 2020-07-07 | Inscripta, Inc. | Nucleic acid-guided nucleases |
IL292895A (en) | 2019-12-18 | 2022-07-01 | Inscripta Inc | Cascade/dcas3 complementation assays for in vivo detection of nucleic acid-directed nuclease-edited cells |
EP4081641A4 (en) * | 2019-12-24 | 2023-12-27 | Asklepios Biopharmaceutical, Inc. | METHOD FOR CONFORMATION DETECTION OF REGULATORY ELEMENTS |
EP4087923A1 (en) * | 2020-01-11 | 2022-11-16 | Inscripta, Inc. | Cell populations with rationally designed edits |
US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
EP4096770A1 (en) | 2020-01-27 | 2022-12-07 | Inscripta, Inc. | Electroporation modules and instrumentation |
US20210317444A1 (en) * | 2020-04-08 | 2021-10-14 | Inscripta, Inc. | System and method for gene editing cassette design |
WO2021211972A1 (en) * | 2020-04-16 | 2021-10-21 | Zymergen Inc. | Circular-permuted nucleic acids for homology-directed editing |
US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
WO2021236740A2 (en) * | 2020-05-19 | 2021-11-25 | Board Of Regents, The University Of Texas System | Genetic physical unclonable functions and methods of use thereof |
WO2021247942A2 (en) * | 2020-06-04 | 2021-12-09 | Inscripta, Inc. | Methods and compositions for crispr editing of cells and correlating the edits to a resulting cellular nucleic acid profile |
JP7419168B2 (ja) * | 2020-06-10 | 2024-01-22 | 株式会社東芝 | 改変型piggyBacトランスポゼースのポリペプチド、それをコードするポリヌクレオチド、導入キャリア、キット、細胞のゲノムに目的配列を組込む方法及び細胞製造方法 |
WO2021257716A2 (en) * | 2020-06-16 | 2021-12-23 | Bio-Techne Corporation | Engineered mad7 directed endonuclease |
WO2022060749A1 (en) * | 2020-09-15 | 2022-03-24 | Inscripta, Inc. | Crispr editing to embed nucleic acid landing pads into genomes of live cells |
US11566241B2 (en) * | 2020-10-02 | 2023-01-31 | Inscripta, Inc. | Methods and systems for modeling of design representation in a library of editing cassettes |
US11512297B2 (en) | 2020-11-09 | 2022-11-29 | Inscripta, Inc. | Affinity tag for recombination protein recruitment |
WO2022146497A1 (en) | 2021-01-04 | 2022-07-07 | Inscripta, Inc. | Mad nucleases |
WO2022150269A1 (en) | 2021-01-07 | 2022-07-14 | Inscripta, Inc. | Mad nucleases |
US11884924B2 (en) | 2021-02-16 | 2024-01-30 | Inscripta, Inc. | Dual strand nucleic acid-guided nickase editing |
CA3209070A1 (en) * | 2021-03-09 | 2022-09-22 | Hongxia Xu | Analyzing expression of protein-coding variants in cells |
WO2022226085A1 (en) * | 2021-04-20 | 2022-10-27 | The Board Of Trustees Of The Leland Stanford Junior University | Compressive molecular probes for genomic editing and tracking |
WO2022261150A2 (en) | 2021-06-09 | 2022-12-15 | Scribe Therapeutics Inc. | Particle delivery systems |
WO2023288018A2 (en) * | 2021-07-14 | 2023-01-19 | Ultima Genomics, Inc. | Barcode selection |
KR20240047475A (ko) * | 2021-09-02 | 2024-04-12 | 유니버시티 오브 워싱톤 | 다중의, 시간적으로 분해된 분자 신호 리코더 및 관련 방법 |
WO2023076134A1 (en) * | 2021-10-26 | 2023-05-04 | Inscripta, Inc. | Processes for measuring strain fitness and/or genotype selection in bioreactors |
CN114022491B (zh) * | 2021-10-27 | 2022-05-10 | 安徽医科大学 | 基于改进空间金字塔模型的小数据集食管癌肿瘤靶区影像自动勾画方法 |
WO2023137233A2 (en) * | 2022-01-17 | 2023-07-20 | Danmarks Tekniske Universitet | Compositions and methods for editing genomes |
EP4384213A1 (en) | 2022-11-02 | 2024-06-19 | Centre For Virology, Vaccinology and Therapeutics Limited | Interferon-producing universal sarbecovirus vaccines, and uses thereof |
Family Cites Families (99)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US1001760A (en) | 1911-03-16 | 1911-08-29 | William F Mcgregor | Floating fish-trap. |
US1001184A (en) | 1911-04-20 | 1911-08-22 | Charles M Coover | Non-slipping device. |
US6562594B1 (en) | 1999-09-29 | 2003-05-13 | Diversa Corporation | Saturation mutagenesis in directed evolution |
US20030044866A1 (en) | 2001-08-15 | 2003-03-06 | Charles Boone | Yeast arrays, methods of making such arrays, and methods of analyzing such arrays |
KR20050010053A (ko) | 2002-06-14 | 2005-01-26 | 다이버사 코포레이션 | 자일라나제, 이를 암호화하는 핵산, 및 이의 제조 및 사용방법 |
JP4447977B2 (ja) | 2004-06-30 | 2010-04-07 | 富士通マイクロエレクトロニクス株式会社 | セキュアプロセッサ、およびセキュアプロセッサ用プログラム。 |
WO2007025097A2 (en) | 2005-08-26 | 2007-03-01 | Danisco A/S | Use |
AU2007258872A1 (en) | 2006-06-16 | 2007-12-21 | Danisco A/S | Bacterium |
WO2008052101A2 (en) | 2006-10-25 | 2008-05-02 | President And Fellows Of Harvard College | Multiplex automated genome engineering |
WO2009032185A2 (en) | 2007-08-28 | 2009-03-12 | The Johns Hopkins University | Functional assay for indentification of loss-of-function mutations in genes |
US20140121118A1 (en) | 2010-11-23 | 2014-05-01 | Opx Biotechnologies, Inc. | Methods, systems and compositions regarding multiplex construction protein amino-acid substitutions and identification of sequence-activity relationships, to provide gene replacement such as with tagged mutant genes, such as via efficient homologous recombination |
WO2012142591A2 (en) | 2011-04-14 | 2012-10-18 | The Regents Of The University Of Colorado | Compositions, methods and uses for multiplex protein sequence activity relationship mapping |
CA3226329A1 (en) | 2011-12-16 | 2013-06-20 | Targetgene Biotechnologies Ltd | Compositions and methods for modifying a predetermined target nucleic acid sequence |
US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
DE202013012241U1 (de) | 2012-05-25 | 2016-01-18 | Emmanuelle Charpentier | Zusammensetzungen für die durch RNA gesteuerte Modifikation einer Ziel-DNA und für die durch RNA gesteuerte Modulation der Transkription |
CN104718284A (zh) | 2012-05-25 | 2015-06-17 | 塞勒克提斯公司 | 工程化用于免疫疗法的异体和免疫抑制耐受性t细胞的方法 |
EP2880171B1 (en) | 2012-08-03 | 2018-10-03 | The Regents of The University of California | Methods and compositions for controlling gene expression by rna processing |
ES2926021T3 (es) | 2012-10-23 | 2022-10-21 | Toolgen Inc | Composición para escindir un ADN objetivo que comprende un ARN guía específico para el ADN objetivo y ácido nucleico codificador de proteína Cas o proteína Cas, y uso de la misma |
PL2928496T3 (pl) | 2012-12-06 | 2020-04-30 | Sigma-Aldrich Co. Llc | Modyfikacja i regulacja genomu w oparciu o CRISPR |
BR112015013784A2 (pt) | 2012-12-12 | 2017-07-11 | Massachusetts Inst Technology | aplicação, manipulação e otimização de sistemas, métodos e composições para manipulação de sequência e aplicações terapêuticas |
MX2015007549A (es) | 2012-12-12 | 2017-01-20 | Broad Inst Inc | Modificaciones de sistemas, métodos y composiciones guía optimizadas para la manipulación de secuencias. |
EP2825654B1 (en) | 2012-12-12 | 2017-04-26 | The Broad Institute, Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
PL2898075T3 (pl) | 2012-12-12 | 2016-09-30 | PROJEKTOWANIE i OPTYMALIZACJA ULEPSZONYCH SYSTEMÓW, SPOSOBY I KOMPOZYCJE ENZYMÓW DO MANIPULACJI SEKWENCJĄ | |
US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
PL2896697T3 (pl) | 2012-12-12 | 2016-01-29 | Broad Inst Inc | Projektowanie systemów, sposoby i optymalizowane kompozycje kierujące do manipulacji sekwencją |
RU2699523C2 (ru) | 2012-12-17 | 2019-09-05 | Президент Энд Фэллоуз Оф Харвард Коллидж | Рнк-направляемая инженерия генома человека |
EP2943579B1 (en) | 2013-01-10 | 2018-09-12 | Dharmacon, Inc. | Libraries and methods for generating molecules |
EP3919505B1 (en) | 2013-01-16 | 2023-08-30 | Emory University | Uses of cas9-nucleic acid complexes |
US10612043B2 (en) | 2013-03-09 | 2020-04-07 | Agilent Technologies, Inc. | Methods of in vivo engineering of large sequences using multiple CRISPR/cas selections of recombineering events |
NZ712727A (en) | 2013-03-14 | 2017-05-26 | Caribou Biosciences Inc | Compositions and methods of nucleic acid-targeting nucleic acids |
US9234213B2 (en) | 2013-03-15 | 2016-01-12 | System Biosciences, Llc | Compositions and methods directed to CRISPR/Cas genomic engineering systems |
KR102210322B1 (ko) | 2013-03-15 | 2021-02-01 | 더 제너럴 하스피탈 코포레이션 | Rna-안내 게놈 편집의 특이성을 증가시키기 위한 rna-안내 foki 뉴클레아제(rfn)의 용도 |
CN105518146B (zh) | 2013-04-04 | 2022-07-15 | 哈佛学院校长同事会 | 利用CRISPR/Cas系统的基因组编辑的治疗性用途 |
EP3004337B1 (en) | 2013-05-29 | 2017-08-02 | Cellectis | Methods for engineering t cells for immunotherapy by using rna-guided cas nuclease system |
KR20160034901A (ko) * | 2013-06-17 | 2016-03-30 | 더 브로드 인스티튜트, 인코퍼레이티드 | 서열 조작에 최적화된 crispr-cas 이중 닉카아제 시스템, 방법 및 조성물 |
SG10201913026TA (en) | 2013-07-09 | 2020-02-27 | Harvard College | Multiplex rna-guided genome engineering |
JP7019233B2 (ja) | 2013-07-11 | 2022-02-15 | モデルナティエックス インコーポレイテッド | CRISPR関連タンパク質をコードする合成ポリヌクレオチドおよび合成sgRNAを含む組成物ならびに使用方法 |
US10563225B2 (en) | 2013-07-26 | 2020-02-18 | President And Fellows Of Harvard College | Genome engineering |
CA2920253A1 (en) | 2013-08-02 | 2015-02-05 | Enevolv, Inc. | Processes and host cells for genome, pathway, and biomolecular engineering |
US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
EP3041498B1 (en) | 2013-09-05 | 2022-02-16 | Massachusetts Institute of Technology | Tuning microbial populations with programmable nucleases |
DE202014010413U1 (de) | 2013-09-18 | 2015-12-08 | Kymab Limited | Zellen und Organismen |
WO2015048577A2 (en) | 2013-09-27 | 2015-04-02 | Editas Medicine, Inc. | Crispr-related methods and compositions |
WO2015048690A1 (en) | 2013-09-27 | 2015-04-02 | The Regents Of The University Of California | Optimized small guide rnas and methods of use |
US20150098954A1 (en) | 2013-10-08 | 2015-04-09 | Elwha Llc | Compositions and Methods Related to CRISPR Targeting |
US10752906B2 (en) | 2013-11-05 | 2020-08-25 | President And Fellows Of Harvard College | Precise microbiota engineering at the cellular level |
JP5900942B2 (ja) | 2013-11-06 | 2016-04-06 | 国立大学法人広島大学 | 核酸挿入用ベクター |
WO2015070062A1 (en) | 2013-11-07 | 2015-05-14 | Massachusetts Institute Of Technology | Cell-based genomic recorded accumulative memory |
EP3071695A2 (en) | 2013-11-18 | 2016-09-28 | Crispr Therapeutics AG | Crispr-cas system materials and methods |
US9074199B1 (en) | 2013-11-19 | 2015-07-07 | President And Fellows Of Harvard College | Mutant Cas9 proteins |
RU2725520C2 (ru) | 2013-12-11 | 2020-07-02 | Регенерон Фармасьютикалс, Инк. | Способы и композиции для направленной модификации генома |
CA2932472A1 (en) | 2013-12-12 | 2015-06-18 | Massachusetts Institute Of Technology | Compositions and methods of use of crispr-cas systems in nucleotide repeat disorders |
US10787654B2 (en) | 2014-01-24 | 2020-09-29 | North Carolina State University | Methods and compositions for sequence guiding Cas9 targeting |
EP4063503A1 (en) | 2014-02-11 | 2022-09-28 | The Regents of the University of Colorado, a body corporate | Crispr enabled multiplexed genome engineering |
CA2939621C (en) | 2014-02-13 | 2019-10-15 | Takara Bio Usa, Inc. | Methods of depleting a target molecule from an initial collection of nucleic acids, and compositions and kits for practicing the same |
WO2015153889A2 (en) | 2014-04-02 | 2015-10-08 | University Of Florida Research Foundation, Incorporated | Materials and methods for the treatment of latent viral infection |
GB201406970D0 (en) | 2014-04-17 | 2014-06-04 | Green Biologics Ltd | Targeted mutations |
GB201406968D0 (en) | 2014-04-17 | 2014-06-04 | Green Biologics Ltd | Deletion mutants |
US20170051311A1 (en) | 2014-05-02 | 2017-02-23 | Tufts University | Methods and apparatus for transformation of naturally competent cells |
US20170175143A1 (en) | 2014-05-20 | 2017-06-22 | Regents Of The University Of Minnesota | Method for editing a genetic sequence |
KR101815695B1 (ko) | 2014-05-28 | 2018-01-08 | 기초과학연구원 | 표적 특이적 뉴클레아제를 이용한 표적 dna의 민감한 검출 방법 |
WO2015188056A1 (en) | 2014-06-05 | 2015-12-10 | Sangamo Biosciences, Inc. | Methods and compositions for nuclease design |
EP3155116A4 (en) | 2014-06-10 | 2017-12-27 | Massachusetts Institute Of Technology | Method for gene editing |
WO2015195798A1 (en) | 2014-06-17 | 2015-12-23 | Poseida Therapeutics, Inc. | A method for directing proteins to specific loci in the genome and uses thereof |
US20150376587A1 (en) | 2014-06-25 | 2015-12-31 | Caribou Biosciences, Inc. | RNA Modification to Engineer Cas9 Activity |
GB201411344D0 (en) | 2014-06-26 | 2014-08-13 | Univ Leicester | Cloning |
US11254933B2 (en) | 2014-07-14 | 2022-02-22 | The Regents Of The University Of California | CRISPR/Cas transcriptional modulation |
US20160053272A1 (en) | 2014-07-18 | 2016-02-25 | Whitehead Institute For Biomedical Research | Methods Of Modifying A Sequence Using CRISPR |
US20160053304A1 (en) | 2014-07-18 | 2016-02-25 | Whitehead Institute For Biomedical Research | Methods Of Depleting Target Sequences Using CRISPR |
US20160076093A1 (en) | 2014-08-04 | 2016-03-17 | University Of Washington | Multiplex homology-directed repair |
CN113789317B (zh) | 2014-08-06 | 2024-02-23 | 基因工具股份有限公司 | 使用空肠弯曲杆菌crispr/cas系统衍生的rna引导的工程化核酸酶的基因编辑 |
US10513711B2 (en) | 2014-08-13 | 2019-12-24 | Dupont Us Holding, Llc | Genetic targeting in non-conventional yeast using an RNA-guided endonuclease |
US20170298450A1 (en) | 2014-09-10 | 2017-10-19 | The Regents Of The University Of California | Reconstruction of ancestral cells by enzymatic recording |
CN113930455A (zh) | 2014-10-09 | 2022-01-14 | 生命技术公司 | Crispr寡核苷酸和基因剪辑 |
US20190100769A1 (en) | 2014-10-31 | 2019-04-04 | Massachusetts Institute Of Technology | Massively parallel combinatorial genetics for crispr |
CA2969619A1 (en) | 2014-12-03 | 2016-06-09 | Agilent Technologies, Inc. | Guide rna with chemical modifications |
MX2017007907A (es) | 2014-12-17 | 2017-09-18 | Du Pont | Composiciones y métodos para la edición genética eficaz en e. coli usando sistemas de ácido desoxirribonucleico (arn) guía/endonucleasa de sistema asociado a repeticiones palindrómicas cortas agrupadas regularmente espaciadas (cas) en combinación con moldes de modificación de polinucleótido circular. |
AU2015364286B2 (en) | 2014-12-20 | 2021-11-04 | Arc Bio, Llc | Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using CRISPR/Cas system proteins |
GB201506509D0 (en) | 2015-04-16 | 2015-06-03 | Univ Wageningen | Nuclease-mediated genome editing |
US20190136248A1 (en) | 2015-05-15 | 2019-05-09 | Pioneer Hi-Bred International, Inc. | Novel guide rna/cas endonuclease systems |
MX2017016289A (es) | 2015-06-18 | 2018-08-15 | Broad Inst Inc | Mutaciones de la enzima crispr que reducen los efectos fuera del blanco. |
US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
EP3313989A4 (en) | 2015-06-29 | 2018-12-05 | Ionis Pharmaceuticals, Inc. | Modified crispr rna and modified single crispr rna and uses thereof |
WO2017015015A1 (en) | 2015-07-17 | 2017-01-26 | Emory University | Crispr-associated protein from francisella and uses related thereto |
EP3329001B1 (en) | 2015-07-28 | 2021-09-22 | Danisco US Inc. | Genome editing systems and methods of use |
WO2017031483A1 (en) | 2015-08-20 | 2017-02-23 | Applied Stemcell, Inc. | Nuclease with enhanced efficiency of genome editing |
EP3353298B1 (en) | 2015-09-21 | 2023-09-13 | Arcturus Therapeutics, Inc. | Allele selective gene editing and uses thereof |
EP3353309A4 (en) | 2015-09-25 | 2019-04-10 | Tarveda Therapeutics, Inc. | COMPOSITIONS AND METHOD FOR GENERIC EDITING |
ES2840648T3 (es) | 2015-10-22 | 2021-07-07 | Inst Nat Sante Rech Med | Generación de código de barras de endonucleasa |
WO2017070598A1 (en) | 2015-10-23 | 2017-04-27 | Caribou Biosciences, Inc. | Engineered crispr class 2 cross-type nucleic-acid targeting nucleic acids |
US11905521B2 (en) | 2015-11-17 | 2024-02-20 | The Chinese University Of Hong Kong | Methods and systems for targeted gene manipulation |
JP2018534942A (ja) | 2015-11-26 | 2018-11-29 | ディーエヌエーイー グループ ホールディングス リミテッド | 単一分子対照 |
KR20190090081A (ko) | 2015-12-07 | 2019-07-31 | 지머젠 인코포레이티드 | Htp 게놈 공학 플랫폼에 의한 미생물 균주 개량 |
US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
WO2017100343A1 (en) | 2015-12-07 | 2017-06-15 | Arc Bio, Llc | Methods and compositions for the making and using of guide nucleic acids |
WO2017109167A2 (en) | 2015-12-24 | 2017-06-29 | B.R.A.I.N. Ag | Reconstitution of dna-end repair pathway in prokaryotes |
LT3474669T (lt) | 2016-06-24 | 2022-06-10 | The Regents Of The University Of Colorado, A Body Corporate | Barkodu pažymėtų kombinatorinių bibliotekų generavimo būdai |
US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
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US11584928B2 (en) | 2023-02-21 |
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AU2017280353A1 (en) | 2019-01-24 |
CA3029254A1 (en) | 2017-12-28 |
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EP3474669A4 (en) | 2019-05-08 |
US20230227810A1 (en) | 2023-07-20 |
US20170369870A1 (en) | 2017-12-28 |
EP3474669B1 (en) | 2022-04-06 |
ES2915562T3 (es) | 2022-06-23 |
WO2017223538A1 (en) | 2017-12-28 |
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