CA2933083A1 - Method for producing retinal pigment epithelial cells - Google Patents

Method for producing retinal pigment epithelial cells Download PDF

Info

Publication number
CA2933083A1
CA2933083A1 CA2933083A CA2933083A CA2933083A1 CA 2933083 A1 CA2933083 A1 CA 2933083A1 CA 2933083 A CA2933083 A CA 2933083A CA 2933083 A CA2933083 A CA 2933083A CA 2933083 A1 CA2933083 A1 CA 2933083A1
Authority
CA
Canada
Prior art keywords
cells
rpe
cultured
days
replated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2933083A
Other languages
English (en)
French (fr)
Inventor
Parul CHOUDHARY
Beata SURMACZ-CORDLE
Heather Dawn Ellen BOOTH
Paul John Whiting
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Ltd Great Britain
Original Assignee
Pfizer Ltd Great Britain
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Ltd Great Britain filed Critical Pfizer Ltd Great Britain
Publication of CA2933083A1 publication Critical patent/CA2933083A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Ophthalmology & Optometry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CA2933083A 2013-12-11 2014-12-08 Method for producing retinal pigment epithelial cells Abandoned CA2933083A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361914445P 2013-12-11 2013-12-11
US61/914,445 2013-12-11
PCT/IB2014/066703 WO2015087231A1 (en) 2013-12-11 2014-12-08 Method for producing retinal pigment epithelial cells

Publications (1)

Publication Number Publication Date
CA2933083A1 true CA2933083A1 (en) 2015-06-18

Family

ID=52446403

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2933083A Abandoned CA2933083A1 (en) 2013-12-11 2014-12-08 Method for producing retinal pigment epithelial cells

Country Status (13)

Country Link
EP (1) EP3080248A1 (https=)
JP (1) JP2017504311A (https=)
KR (1) KR101871084B1 (https=)
CN (1) CN105814194A (https=)
AR (1) AR098693A1 (https=)
AU (1) AU2014363032A1 (https=)
BR (1) BR112016012129A8 (https=)
CA (1) CA2933083A1 (https=)
HK (1) HK1221734A1 (https=)
IL (1) IL245594A0 (https=)
MX (1) MX2016007671A (https=)
TW (2) TW201538726A (https=)
WO (1) WO2015087231A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106906178A (zh) * 2017-04-07 2017-06-30 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 培养上皮细胞的组合物及其保持上皮样细胞形态中的应用

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3221318B1 (en) * 2014-11-21 2021-08-25 Rigel Pharmaceuticals, Inc. Fused imidazole derivatives as tgf-beta inhibitors
KR20170115514A (ko) 2014-12-30 2017-10-17 셀 큐어 뉴로사이언시스 리미티드 Rpe 세포 집단 및 이를 생성하는 방법
WO2016108240A1 (en) * 2014-12-30 2016-07-07 Cell Cure Neurosciences Ltd. Assessing retinal pigment epithelial cell populations
CN114533761A (zh) * 2014-12-30 2022-05-27 细胞治疗神经科学有限公司 治疗视网膜疾病的方法
WO2017021973A1 (en) 2015-08-05 2017-02-09 Cell Cure Neurosciences Ltd. Preparation of retinal pigment epithelium cells
CN114807035B (zh) * 2015-09-08 2024-02-02 (由卫生与公众服务部部长代表的)美利坚合众国 临床级别视网膜色素上皮细胞的可再现的分化方法
KR20260003355A (ko) 2015-09-08 2026-01-06 후지필름 셀룰러 다이내믹스, 인코포레이티드 줄기 세포에서 유래된 망막색소상피의 macs 기반 정제
CN108431211B (zh) 2015-10-20 2026-03-24 富士胶片细胞动力公司 通过遗传编程的多谱系造血前体细胞产生
EP3384008A4 (en) 2015-12-04 2019-10-02 Bond University Ltd Methods of differentiating retinal cells
CN106344932B (zh) * 2016-08-26 2019-12-27 首都医科大学附属北京同仁医院 一种用于提高视网膜退行性疾病基因治疗效果的组合物及其应用
MX2019006576A (es) 2016-12-07 2019-08-21 Mayo Found Medical Education & Res Metodos y materiales para usar soportes de fibrina para trasplante de epitelio de pigmento retiniano.
WO2018195175A1 (en) 2017-04-18 2018-10-25 FUJIFILM Cellular Dynamics, Inc. Antigen-specific immune effector cells
JP7311433B2 (ja) * 2017-06-05 2023-07-19 メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ 幹細胞を培養し、増殖させ、分化させるための方法及び材料
CN109957545A (zh) 2017-12-26 2019-07-02 江苏艾尔康生物医药科技有限公司 一种消化rpe细胞的方法
BR112020013238A2 (pt) 2017-12-29 2020-12-01 Cell Cure Neurosciences Ltd. composição e método para formular células do epitélio pigmentar da retina bem como uso da dita composição para tratar uma condição retiniana
WO2020003219A1 (en) * 2018-06-29 2020-01-02 Oat & Iil India Laboratories Private Limited Substituted pyrazole derivatives as insecticides and fungicides
JP2022532411A (ja) * 2019-05-15 2022-07-14 ノヴォ ノルディスク アー／エス ヒト多能性幹細胞から眼野前駆細胞を得るための方法
CN114450418A (zh) * 2019-07-19 2022-05-06 东京毅力科创株式会社 细胞的分化状态的评价方法
JP7668013B2 (ja) 2019-07-26 2025-04-24 学校法人 埼玉医科大学 Ａｌｋ２／ａｃｖｒ１の細胞外領域を認識する抗体
CN113699097B (zh) * 2021-09-08 2023-06-09 湖南光琇高新生命科技有限公司 视网膜色素上皮细胞的制备方法
CN120905148B (zh) * 2025-10-11 2026-01-06 中国人民解放军陆军军医大学第一附属医院 一种基于ChR2和GCaMP5G双转染hESC构建起搏细胞制备视网膜类器官的方法

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG49267A1 (en) 1989-08-14 1998-05-18 Photogenesis Inc Surgical instrument and cell isolation and transplantation
US6045791A (en) 1992-03-06 2000-04-04 Photogenesis, Inc. Retinal pigment epithelium transplantation
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5941250A (en) 1996-11-21 1999-08-24 University Of Louisville Research Foundation Inc. Retinal tissue implantation method
WO2004033635A2 (en) 2002-10-04 2004-04-22 Tissuetech, Inc. Retinal pigment epithelial cell cultures on amniotic membrane and transplantation
BRPI0507074A (pt) 2004-01-23 2007-06-19 Advanced Cell Tech Inc modalidades aperfeiçoadas para o tratamento de doenças degenerativas da retina
CA3015835A1 (en) 2004-11-04 2006-05-18 Astellas Institute For Regenerative Medicine Derivation of embryonic stem cells
US7893315B2 (en) 2004-11-04 2011-02-22 Advanced Cell Technology, Inc. Derivation of embryonic stem cells and embryo-derived cells
BRPI0619794B8 (pt) 2005-12-13 2022-06-14 Univ Kyoto Uso de um fator de reprogramação, agente para a preparação de uma célula-tronco pluripotente induzida a partir de uma célula somática e métodos para preparar uma célula- tronco pluripotente induzida método e para preparar uma célula somática e uso de células-tronco pluripotentes induzidas
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
JP5441099B2 (ja) 2007-01-18 2014-03-12 独立行政法人理化学研究所 視細胞への分化誘導方法
DK3399025T3 (da) 2007-03-23 2025-06-16 Wisconsin Alumni Res Found Omprogrammering af somatiske celler
ES2527444T5 (es) * 2007-04-18 2018-05-17 Hadasit Medical Research Services & Development Limited Células del epitelio pigmentario de la retina derivadas de células madre
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
IL292561A (en) 2007-10-12 2022-06-01 Astellas Inst For Regenerative Medicine Improved methods of rpe cell production and rpe cell preparations
GB0806746D0 (en) 2008-04-14 2008-05-14 Ucl Business Plc Membrane
WO2011028524A1 (en) 2009-08-24 2011-03-10 Wisconsin Alumni Research Foundation Substantially pure human retinal progenitor, forebrain progenitor, and retinal pigment epithelium cell cultures and methods of making the same
US10485829B2 (en) 2009-11-17 2019-11-26 Astellas Institute For Regenerative Medicine Methods of producing human RPE cells and pharmaceutical preparations of human RPE cells
EP2383333B1 (en) 2010-04-28 2015-05-27 Technische Universität Dresden Method for producing polarized retinal progenitor cells from pluripotent stem cells and their differentiation into retinal pigment epithelium cells
US9453198B2 (en) * 2010-05-25 2016-09-27 Memorial Sloan Kettering Cancer Center Method of nociceptor differentiation of human embryonic stem cells and uses thereof
GB201011313D0 (en) 2010-07-05 2010-08-18 Ucl Business Plc Implantation devices, methods and implants
AU2011279250B2 (en) 2010-07-12 2014-12-04 California Institute Of Technology Biocompatible substrate for facilitating interconnections between stem cells and target tissues and methods for implanting same
US8871744B2 (en) 2010-07-21 2014-10-28 B & G Partyers, LLC Compounds and methods for selectively targeting tumor-associated mucins
CN102465111A (zh) * 2010-11-19 2012-05-23 薛志刚 一种体外人胚胎干细胞诱导分化为视网膜色素上皮细胞的试验方法
US10390856B2 (en) 2011-01-18 2019-08-27 Minipumps, Llc Surgical implantation instrument
WO2012177968A1 (en) 2011-06-22 2012-12-27 The Schepens Eye Research Institute, Inc. A scaffold for subretinal cell transplantation and drug delivery
TWI719277B (zh) 2011-11-14 2021-02-21 美商安斯泰來再生醫藥協會 人類rpe細胞之醫藥組合物及其用途
US9850463B2 (en) 2012-02-01 2017-12-26 The Regents Of The University Of California Methods of culturing retinal pigmented epithelium cells, including xeno-free production, RPE enrichment, and cryopreservation
CN102618488A (zh) * 2012-03-15 2012-08-01 中国人民解放军第三军医大学第一附属医院 一种制备视网膜色素上皮细胞的方法
US9458428B2 (en) 2012-06-05 2016-10-04 The Regents Of The University Of California Methods and compositions for the rapid production of retinal pigmented epithelial cells from pluripotent cells
EP2951290B1 (en) 2013-02-01 2017-11-29 The United States of America, as represented by The Secretary, Department of Health and Human Services Method for generating retinal pigment epithelium (rpe) cells from induced pluripotent stem cells (ipscs)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106906178A (zh) * 2017-04-07 2017-06-30 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 培养上皮细胞的组合物及其保持上皮样细胞形态中的应用

Also Published As

Publication number Publication date
EP3080248A1 (en) 2016-10-19
TW201538726A (zh) 2015-10-16
CN105814194A (zh) 2016-07-27
AU2014363032A1 (en) 2016-06-09
JP2017504311A (ja) 2017-02-09
BR112016012129A2 (pt) 2017-08-08
BR112016012129A8 (pt) 2020-05-12
HK1221734A1 (zh) 2017-06-09
IL245594A0 (en) 2016-06-30
WO2015087231A1 (en) 2015-06-18
KR20160095118A (ko) 2016-08-10
MX2016007671A (es) 2016-10-14
TW201823451A (zh) 2018-07-01
KR101871084B1 (ko) 2018-06-25
AR098693A1 (es) 2016-06-08

Similar Documents

Publication Publication Date Title
CA2933083A1 (en) Method for producing retinal pigment epithelial cells
US20150159134A1 (en) Method for producing retinal pigment epithelial cells
US20240067924A1 (en) Specification of functional cranial placode derivatives from human pluripotent stem cells
JP5721111B2 (ja) 幹細胞の培地及び培養方法
EP2314671B1 (en) Method for culture of stem cell
KR102481035B1 (ko) 선하수체 또는 이의 전구 조직의 제조 방법
AU2017214749B2 (en) Methods of differentiating stem cell-derived ectodermal lineage precursors
JP7737672B2 (ja) 下垂体ホルモン産生細胞及びその前駆細胞の分離法
AU2018327341A1 (en) Methods of differentiating stem cell-derived ectodermal lineage precursors
WO2024207102A1 (en) Compositions and methods for differentiating rpe cells

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20160608

FZDE Discontinued

Effective date: 20210104