KR101871084B1 - 망막 색소 상피 세포의 제조 방법 - Google Patents

망막 색소 상피 세포의 제조 방법 Download PDF

Info

Publication number
KR101871084B1
KR101871084B1 KR1020167018138A KR20167018138A KR101871084B1 KR 101871084 B1 KR101871084 B1 KR 101871084B1 KR 1020167018138 A KR1020167018138 A KR 1020167018138A KR 20167018138 A KR20167018138 A KR 20167018138A KR 101871084 B1 KR101871084 B1 KR 101871084B1
Authority
KR
South Korea
Prior art keywords
cells
delete delete
days
rpe
cultured
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
KR1020167018138A
Other languages
English (en)
Korean (ko)
Other versions
KR20160095118A (ko
Inventor
파룰 초우다리
베타 서막츠-코들
헤더 돈 엘런 부쓰
폴 존 와이팅
Original Assignee
화이자 리미티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 화이자 리미티드 filed Critical 화이자 리미티드
Publication of KR20160095118A publication Critical patent/KR20160095118A/ko
Application granted granted Critical
Publication of KR101871084B1 publication Critical patent/KR101871084B1/ko
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0618Cells of the nervous system
    • C12N5/0621Eye cells, e.g. cornea, iris pigmented cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00Drugs for disorders of the senses
    • A61P27/02Ophthalmic agents
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/15Transforming growth factor beta (TGF-β)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/155Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/16Activin; Inhibin; Mullerian inhibiting substance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins
    • C12N2533/52Fibronectin; Laminin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/90Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Ophthalmology & Optometry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
KR1020167018138A 2013-12-11 2014-12-08 망막 색소 상피 세포의 제조 방법 Expired - Fee Related KR101871084B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361914445P 2013-12-11 2013-12-11
US61/914,445 2013-12-11
PCT/IB2014/066703 WO2015087231A1 (en) 2013-12-11 2014-12-08 Method for producing retinal pigment epithelial cells

Publications (2)

Publication Number Publication Date
KR20160095118A KR20160095118A (ko) 2016-08-10
KR101871084B1 true KR101871084B1 (ko) 2018-06-25

Family

ID=52446403

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020167018138A Expired - Fee Related KR101871084B1 (ko) 2013-12-11 2014-12-08 망막 색소 상피 세포의 제조 방법

Country Status (13)

Country Link
EP (1) EP3080248A1 (https=)
JP (1) JP2017504311A (https=)
KR (1) KR101871084B1 (https=)
CN (1) CN105814194A (https=)
AR (1) AR098693A1 (https=)
AU (1) AU2014363032A1 (https=)
BR (1) BR112016012129A8 (https=)
CA (1) CA2933083A1 (https=)
HK (1) HK1221734A1 (https=)
IL (1) IL245594A0 (https=)
MX (1) MX2016007671A (https=)
TW (2) TW201538726A (https=)
WO (1) WO2015087231A1 (https=)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3221318B1 (en) * 2014-11-21 2021-08-25 Rigel Pharmaceuticals, Inc. Fused imidazole derivatives as tgf-beta inhibitors
KR20170115514A (ko) 2014-12-30 2017-10-17 셀 큐어 뉴로사이언시스 리미티드 Rpe 세포 집단 및 이를 생성하는 방법
WO2016108240A1 (en) * 2014-12-30 2016-07-07 Cell Cure Neurosciences Ltd. Assessing retinal pigment epithelial cell populations
CN114533761A (zh) * 2014-12-30 2022-05-27 细胞治疗神经科学有限公司 治疗视网膜疾病的方法
WO2017021973A1 (en) 2015-08-05 2017-02-09 Cell Cure Neurosciences Ltd. Preparation of retinal pigment epithelium cells
CN114807035B (zh) * 2015-09-08 2024-02-02 (由卫生与公众服务部部长代表的)美利坚合众国 临床级别视网膜色素上皮细胞的可再现的分化方法
KR20260003355A (ko) 2015-09-08 2026-01-06 후지필름 셀룰러 다이내믹스, 인코포레이티드 줄기 세포에서 유래된 망막색소상피의 macs 기반 정제
CN108431211B (zh) 2015-10-20 2026-03-24 富士胶片细胞动力公司 通过遗传编程的多谱系造血前体细胞产生
EP3384008A4 (en) 2015-12-04 2019-10-02 Bond University Ltd Methods of differentiating retinal cells
CN106344932B (zh) * 2016-08-26 2019-12-27 首都医科大学附属北京同仁医院 一种用于提高视网膜退行性疾病基因治疗效果的组合物及其应用
MX2019006576A (es) 2016-12-07 2019-08-21 Mayo Found Medical Education & Res Metodos y materiales para usar soportes de fibrina para trasplante de epitelio de pigmento retiniano.
CN106906178A (zh) * 2017-04-07 2017-06-30 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 培养上皮细胞的组合物及其保持上皮样细胞形态中的应用
WO2018195175A1 (en) 2017-04-18 2018-10-25 FUJIFILM Cellular Dynamics, Inc. Antigen-specific immune effector cells
JP7311433B2 (ja) * 2017-06-05 2023-07-19 メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ 幹細胞を培養し、増殖させ、分化させるための方法及び材料
CN109957545A (zh) 2017-12-26 2019-07-02 江苏艾尔康生物医药科技有限公司 一种消化rpe细胞的方法
BR112020013238A2 (pt) 2017-12-29 2020-12-01 Cell Cure Neurosciences Ltd. composição e método para formular células do epitélio pigmentar da retina bem como uso da dita composição para tratar uma condição retiniana
WO2020003219A1 (en) * 2018-06-29 2020-01-02 Oat & Iil India Laboratories Private Limited Substituted pyrazole derivatives as insecticides and fungicides
JP2022532411A (ja) * 2019-05-15 2022-07-14 ノヴォ ノルディスク アー/エス ヒト多能性幹細胞から眼野前駆細胞を得るための方法
CN114450418A (zh) * 2019-07-19 2022-05-06 东京毅力科创株式会社 细胞的分化状态的评价方法
JP7668013B2 (ja) 2019-07-26 2025-04-24 学校法人 埼玉医科大学 Alk2/acvr1の細胞外領域を認識する抗体
CN113699097B (zh) * 2021-09-08 2023-06-09 湖南光琇高新生命科技有限公司 视网膜色素上皮细胞的制备方法
CN120905148B (zh) * 2025-10-11 2026-01-06 中国人民解放军陆军军医大学第一附属医院 一种基于ChR2和GCaMP5G双转染hESC构建起搏细胞制备视网膜类器官的方法

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG49267A1 (en) 1989-08-14 1998-05-18 Photogenesis Inc Surgical instrument and cell isolation and transplantation
US6045791A (en) 1992-03-06 2000-04-04 Photogenesis, Inc. Retinal pigment epithelium transplantation
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5941250A (en) 1996-11-21 1999-08-24 University Of Louisville Research Foundation Inc. Retinal tissue implantation method
WO2004033635A2 (en) 2002-10-04 2004-04-22 Tissuetech, Inc. Retinal pigment epithelial cell cultures on amniotic membrane and transplantation
BRPI0507074A (pt) 2004-01-23 2007-06-19 Advanced Cell Tech Inc modalidades aperfeiçoadas para o tratamento de doenças degenerativas da retina
CA3015835A1 (en) 2004-11-04 2006-05-18 Astellas Institute For Regenerative Medicine Derivation of embryonic stem cells
US7893315B2 (en) 2004-11-04 2011-02-22 Advanced Cell Technology, Inc. Derivation of embryonic stem cells and embryo-derived cells
BRPI0619794B8 (pt) 2005-12-13 2022-06-14 Univ Kyoto Uso de um fator de reprogramação, agente para a preparação de uma célula-tronco pluripotente induzida a partir de uma célula somática e métodos para preparar uma célula- tronco pluripotente induzida método e para preparar uma célula somática e uso de células-tronco pluripotentes induzidas
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
JP5441099B2 (ja) 2007-01-18 2014-03-12 独立行政法人理化学研究所 視細胞への分化誘導方法
DK3399025T3 (da) 2007-03-23 2025-06-16 Wisconsin Alumni Res Found Omprogrammering af somatiske celler
ES2527444T5 (es) * 2007-04-18 2018-05-17 Hadasit Medical Research Services & Development Limited Células del epitelio pigmentario de la retina derivadas de células madre
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
IL292561A (en) 2007-10-12 2022-06-01 Astellas Inst For Regenerative Medicine Improved methods of rpe cell production and rpe cell preparations
GB0806746D0 (en) 2008-04-14 2008-05-14 Ucl Business Plc Membrane
WO2011028524A1 (en) 2009-08-24 2011-03-10 Wisconsin Alumni Research Foundation Substantially pure human retinal progenitor, forebrain progenitor, and retinal pigment epithelium cell cultures and methods of making the same
US10485829B2 (en) 2009-11-17 2019-11-26 Astellas Institute For Regenerative Medicine Methods of producing human RPE cells and pharmaceutical preparations of human RPE cells
EP2383333B1 (en) 2010-04-28 2015-05-27 Technische Universität Dresden Method for producing polarized retinal progenitor cells from pluripotent stem cells and their differentiation into retinal pigment epithelium cells
US9453198B2 (en) * 2010-05-25 2016-09-27 Memorial Sloan Kettering Cancer Center Method of nociceptor differentiation of human embryonic stem cells and uses thereof
GB201011313D0 (en) 2010-07-05 2010-08-18 Ucl Business Plc Implantation devices, methods and implants
AU2011279250B2 (en) 2010-07-12 2014-12-04 California Institute Of Technology Biocompatible substrate for facilitating interconnections between stem cells and target tissues and methods for implanting same
US8871744B2 (en) 2010-07-21 2014-10-28 B & G Partyers, LLC Compounds and methods for selectively targeting tumor-associated mucins
CN102465111A (zh) * 2010-11-19 2012-05-23 薛志刚 一种体外人胚胎干细胞诱导分化为视网膜色素上皮细胞的试验方法
US10390856B2 (en) 2011-01-18 2019-08-27 Minipumps, Llc Surgical implantation instrument
WO2012177968A1 (en) 2011-06-22 2012-12-27 The Schepens Eye Research Institute, Inc. A scaffold for subretinal cell transplantation and drug delivery
TWI719277B (zh) 2011-11-14 2021-02-21 美商安斯泰來再生醫藥協會 人類rpe細胞之醫藥組合物及其用途
US9850463B2 (en) 2012-02-01 2017-12-26 The Regents Of The University Of California Methods of culturing retinal pigmented epithelium cells, including xeno-free production, RPE enrichment, and cryopreservation
CN102618488A (zh) * 2012-03-15 2012-08-01 中国人民解放军第三军医大学第一附属医院 一种制备视网膜色素上皮细胞的方法
US9458428B2 (en) 2012-06-05 2016-10-04 The Regents Of The University Of California Methods and compositions for the rapid production of retinal pigmented epithelial cells from pluripotent cells
EP2951290B1 (en) 2013-02-01 2017-11-29 The United States of America, as represented by The Secretary, Department of Health and Human Services Method for generating retinal pigment epithelium (rpe) cells from induced pluripotent stem cells (ipscs)

Also Published As

Publication number Publication date
EP3080248A1 (en) 2016-10-19
TW201538726A (zh) 2015-10-16
CN105814194A (zh) 2016-07-27
AU2014363032A1 (en) 2016-06-09
JP2017504311A (ja) 2017-02-09
BR112016012129A2 (pt) 2017-08-08
BR112016012129A8 (pt) 2020-05-12
HK1221734A1 (zh) 2017-06-09
IL245594A0 (en) 2016-06-30
CA2933083A1 (en) 2015-06-18
WO2015087231A1 (en) 2015-06-18
KR20160095118A (ko) 2016-08-10
MX2016007671A (es) 2016-10-14
TW201823451A (zh) 2018-07-01
AR098693A1 (es) 2016-06-08

Similar Documents

Publication Publication Date Title
KR101871084B1 (ko) 망막 색소 상피 세포의 제조 방법
US20150159134A1 (en) Method for producing retinal pigment epithelial cells
JP5721111B2 (ja) 幹細胞の培地及び培養方法
JP5631210B2 (ja) 多能性幹細胞からのニューロン細胞の生成
AU2014352773B2 (en) Specification of functional cranial placode derivatives from human pluripotent stem cells
AU2017214749B2 (en) Methods of differentiating stem cell-derived ectodermal lineage precursors
JPWO2016013669A1 (ja) 腺性下垂体又はその前駆組織の製造方法
JP7766724B2 (ja) オリゴデンドロサイト前駆細胞組成物
Saito‐Diaz et al. Autonomic neurons with sympathetic character derived from human pluripotent stem cells
IL297082A (en) Methods for producing thymus cells in vitro
US20200199530A1 (en) Methods of differentiating stem cell-derived ectodermal lineage precursors
JP7737672B2 (ja) 下垂体ホルモン産生細胞及びその前駆細胞の分離法
AU2022355700A1 (en) Cell aggregate including pituitary hormone-producing cells and method for producing same
Rattier et al. Disruption of epithelial integrity drives mesendoderm differentiation in human induced pluripotent stem cells by enabling BMP and ACTIVIN sensing
EP4689063A1 (en) Compositions and methods for differentiating rpe cells
HK40091526A (zh) 少突胶质细胞祖细胞组合物
HK40086565A (en) Method for separating pituitary hormone-producing cells and progenitor cells thereof

Legal Events

Date Code Title Description
A201 Request for examination
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PC1903 Unpaid annual fee

St.27 status event code: A-4-4-U10-U13-oth-PC1903

Not in force date: 20210620

Payment event data comment text: Termination Category : DEFAULT_OF_REGISTRATION_FEE

PC1903 Unpaid annual fee

St.27 status event code: N-4-6-H10-H13-oth-PC1903

Ip right cessation event data comment text: Termination Category : DEFAULT_OF_REGISTRATION_FEE

Not in force date: 20210620