CN105814194A - 产生视网膜色素上皮细胞的方法 - Google Patents

产生视网膜色素上皮细胞的方法 Download PDF

Info

Publication number
CN105814194A
CN105814194A CN201480067617.3A CN201480067617A CN105814194A CN 105814194 A CN105814194 A CN 105814194A CN 201480067617 A CN201480067617 A CN 201480067617A CN 105814194 A CN105814194 A CN 105814194A
Authority
CN
China
Prior art keywords
cells
rpe
cultured
days
certain embodiments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201480067617.3A
Other languages
English (en)
Chinese (zh)
Inventor
P·乔杜里
B·苏尔马齐-科德勒
H·D·E·布思
P·J·惠廷
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Ltd Great Britain
Original Assignee
Pfizer Ltd Great Britain
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Ltd Great Britain filed Critical Pfizer Ltd Great Britain
Publication of CN105814194A publication Critical patent/CN105814194A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Ophthalmology & Optometry (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN201480067617.3A 2013-12-11 2014-12-08 产生视网膜色素上皮细胞的方法 Pending CN105814194A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361914445P 2013-12-11 2013-12-11
US61/914,445 2013-12-11
PCT/IB2014/066703 WO2015087231A1 (en) 2013-12-11 2014-12-08 Method for producing retinal pigment epithelial cells

Publications (1)

Publication Number Publication Date
CN105814194A true CN105814194A (zh) 2016-07-27

Family

ID=52446403

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480067617.3A Pending CN105814194A (zh) 2013-12-11 2014-12-08 产生视网膜色素上皮细胞的方法

Country Status (14)

Country Link
US (1) US20150159134A1 (https=)
EP (1) EP3080248A1 (https=)
JP (1) JP2017504311A (https=)
KR (1) KR101871084B1 (https=)
CN (1) CN105814194A (https=)
AR (1) AR098693A1 (https=)
AU (1) AU2014363032A1 (https=)
BR (1) BR112016012129A8 (https=)
CA (1) CA2933083A1 (https=)
HK (1) HK1221734A1 (https=)
IL (1) IL245594A0 (https=)
MX (1) MX2016007671A (https=)
TW (2) TW201823451A (https=)
WO (1) WO2015087231A1 (https=)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106344932A (zh) * 2016-08-26 2017-01-25 首都医科大学附属北京同仁医院 一种用于提高视网膜退行性疾病基因治疗效果的组合物及其应用
CN114450418A (zh) * 2019-07-19 2022-05-06 东京毅力科创株式会社 细胞的分化状态的评价方法
WO2023035608A1 (zh) * 2021-09-08 2023-03-16 湖南光琇高新生命科技有限公司 视网膜色素上皮细胞的制备方法
CN120905148A (zh) * 2025-10-11 2025-11-07 中国人民解放军陆军军医大学第一附属医院 一种基于ChR2和GCaMP5G双转染hESC构建起搏细胞制备视网膜类器官的方法

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL281453B (en) 2009-11-17 2022-07-01 Astellas Inst For Regenerative Medicine Methods for preparing human rpe cells and pharmaceutical preparations of human rpe cells
EP3221318B1 (en) * 2014-11-21 2021-08-25 Rigel Pharmaceuticals, Inc. Fused imidazole derivatives as tgf-beta inhibitors
EP3240612B1 (en) * 2014-12-30 2020-12-23 Cell Cure Neurosciences Ltd. Methods of treating retinal diseases
CA2972575A1 (en) * 2014-12-30 2016-07-07 Cell Cure Neurosciences Ltd. Rpe cell populations and methods of generating same
EP3916085A1 (en) * 2014-12-30 2021-12-01 Cell Cure Neurosciences Ltd. Assessing retinal pigment epithelial cell populations
CN108290855A (zh) 2015-07-01 2018-07-17 西北大学 被取代的喹唑啉化合物及其调节葡糖脑苷脂酶活性的用途
US11066642B2 (en) 2015-08-05 2021-07-20 Cell Cure Neurosciences Ltd Preparation of retinal pigment epithelium cells
AU2016318774B2 (en) * 2015-09-08 2022-08-18 FUJIFILM Cellular Dynamics, Inc. MACS-based purification of stem cell-derived retinal pigment epithelium
SG11201801770VA (en) 2015-09-08 2018-04-27 Sumitomo Dainippon Pharma Co Ltd Method for producing retinal pigment epithelial cells
DK3347456T3 (da) * 2015-09-08 2024-02-19 Us Health Fremgangsmåde til reproducerbar differentiering af retinale pigmentepitelceller af klinisk kvalitet
US10947502B2 (en) 2015-10-20 2021-03-16 FUJIFILM Cellular Dynamics, Inc. Methods for directed differentiation of pluripotent stem cells to immune cells
US20180362925A1 (en) * 2015-12-04 2018-12-20 Bond University Ltd Methods for differentiating cells
JP2020501543A (ja) 2016-12-07 2020-01-23 メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ 網膜色素上皮移植のためのフィブリン支持体を用いた方法及び材料
CN110520139A (zh) * 2017-02-08 2019-11-29 哈达西特医疗研究服务和开发有限公司 用于治疗视网膜疾病的感光细胞
WO2018164240A1 (ja) 2017-03-08 2018-09-13 大日本住友製薬株式会社 網膜色素上皮細胞の製造方法
CN106906178A (zh) * 2017-04-07 2017-06-30 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 培养上皮细胞的组合物及其保持上皮样细胞形态中的应用
SG11201909331UA (en) 2017-04-18 2019-11-28 Fujifilm Cellular Dynamics Inc Antigen-specific immune effector cells
KR102624998B1 (ko) * 2017-06-05 2024-01-12 메이오 파운데이션 포 메디칼 에쥬케이션 앤드 리써치 줄기 세포의 배양, 증식 및 분화를 위한 방법 및 물질
EP3687580A1 (en) * 2017-09-27 2020-08-05 Sigilon Therapeutics, Inc. Methods, compositions, and implantable elements comprising active cells
IL263217B (en) 2017-11-24 2022-06-01 Juul Labs Inc Puff sensing and power circuitry for vaporizer devices
CN109957545A (zh) 2017-12-26 2019-07-02 江苏艾尔康生物医药科技有限公司 一种消化rpe细胞的方法
WO2019130061A2 (en) 2017-12-29 2019-07-04 Cell Cure Neurosciences Ltd. Retinal pigment epithelium cell compositions
WO2020003219A1 (en) * 2018-06-29 2020-01-02 Oat & Iil India Laboratories Private Limited Substituted pyrazole derivatives as insecticides and fungicides
EP3969570A1 (en) * 2019-05-15 2022-03-23 Novo Nordisk A/S Methods for obtaining eye field progenitor cells from human pluripotent stem cells
EP4008350A4 (en) 2019-07-26 2023-06-14 Saitama Medical University ANTIBODY RECOGNIZING THE EXTRACELLULAR REGION OF ALK2/ACVR1

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101688178A (zh) * 2007-04-18 2010-03-31 哈达锡特医学研究服务及发展有限公司 干细胞衍生的视网膜色素上皮细胞
CA2800500A1 (en) * 2010-05-25 2011-12-01 Memorial Sloan-Kettering Cancer Center Method of nociceptor differentiation of human embryonic stem cells and uses thereof
CN102465111A (zh) * 2010-11-19 2012-05-23 薛志刚 一种体外人胚胎干细胞诱导分化为视网膜色素上皮细胞的试验方法
CN102618488A (zh) * 2012-03-15 2012-08-01 中国人民解放军第三军医大学第一附属医院 一种制备视网膜色素上皮细胞的方法

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG49267A1 (en) 1989-08-14 1998-05-18 Photogenesis Inc Surgical instrument and cell isolation and transplantation
US6045791A (en) 1992-03-06 2000-04-04 Photogenesis, Inc. Retinal pigment epithelium transplantation
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5941250A (en) 1996-11-21 1999-08-24 University Of Louisville Research Foundation Inc. Retinal tissue implantation method
CN1720055A (zh) 2002-10-04 2006-01-11 组织技术公司 视网膜上皮细胞在羊膜上的培养和移植
EP2929782A1 (en) 2004-01-23 2015-10-14 Ocata Therapeutics, Inc. Improved modalities for the treatment of degenerative diseases of the retina
US7893315B2 (en) 2004-11-04 2011-02-22 Advanced Cell Technology, Inc. Derivation of embryonic stem cells and embryo-derived cells
US7838727B2 (en) 2004-11-04 2010-11-23 Advanced Cell Technology, Inc. Derivation of embryonic stem cells
US8048999B2 (en) 2005-12-13 2011-11-01 Kyoto University Nuclear reprogramming factor
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
EP2128244A4 (en) 2007-01-18 2012-05-09 Riken PROCESS FOR INDUCTION / DIFFERENTIATION TO A PHOTOREZEPTORZELLE
JP5813321B2 (ja) 2007-03-23 2015-11-17 ウィスコンシン アラムニ リサーチ ファンデーション 体細胞の再プログラム化
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
DK2209888T3 (da) 2007-10-12 2020-01-20 Astellas Inst For Regenerative Medicine Forbedrede fremgangsmåder til fremstilling af rpe-celler og sammensætninger af rpe-celler
GB0806746D0 (en) 2008-04-14 2008-05-14 Ucl Business Plc Membrane
EP2470645B1 (en) 2009-08-24 2019-06-26 Wisconsin Alumni Research Foundation Substantially pure human retinal progenitor, forebrain progenitor, and retinal pigment epithelium cell cultures and methods of making the same
IL281453B (en) 2009-11-17 2022-07-01 Astellas Inst For Regenerative Medicine Methods for preparing human rpe cells and pharmaceutical preparations of human rpe cells
ES2542226T3 (es) 2010-04-28 2015-08-03 Technische Universität Dresden Método para producir células progenitoras retinianas polarizadas a partir de células madre pluripotentes y su diferenciación para dar células del epitelio pigmentario retiniano
GB201011313D0 (en) 2010-07-05 2010-08-18 Ucl Business Plc Implantation devices, methods and implants
EP2593117B1 (en) 2010-07-12 2019-03-20 University of Southern California Biocompatible substrate for facilitating interconnections between stem cells and target tissues and methods for implanting same
US8871744B2 (en) 2010-07-21 2014-10-28 B & G Partyers, LLC Compounds and methods for selectively targeting tumor-associated mucins
WO2012099873A1 (en) 2011-01-18 2012-07-26 Minipums, Llc Surgical implantation instrument
WO2012177968A1 (en) 2011-06-22 2012-12-27 The Schepens Eye Research Institute, Inc. A scaffold for subretinal cell transplantation and drug delivery
IL287381B2 (en) 2011-11-14 2024-04-01 Astellas Inst For Regenerative Medicine Pharmaceutical preparations of human rpe cells and uses thereof
US9850463B2 (en) 2012-02-01 2017-12-26 The Regents Of The University Of California Methods of culturing retinal pigmented epithelium cells, including xeno-free production, RPE enrichment, and cryopreservation
US9458428B2 (en) 2012-06-05 2016-10-04 The Regents Of The University Of California Methods and compositions for the rapid production of retinal pigmented epithelial cells from pluripotent cells
EP2951290B1 (en) 2013-02-01 2017-11-29 The United States of America, as represented by The Secretary, Department of Health and Human Services Method for generating retinal pigment epithelium (rpe) cells from induced pluripotent stem cells (ipscs)

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101688178A (zh) * 2007-04-18 2010-03-31 哈达锡特医学研究服务及发展有限公司 干细胞衍生的视网膜色素上皮细胞
CA2800500A1 (en) * 2010-05-25 2011-12-01 Memorial Sloan-Kettering Cancer Center Method of nociceptor differentiation of human embryonic stem cells and uses thereof
CN102465111A (zh) * 2010-11-19 2012-05-23 薛志刚 一种体外人胚胎干细胞诱导分化为视网膜色素上皮细胞的试验方法
CN102618488A (zh) * 2012-03-15 2012-08-01 中国人民解放军第三军医大学第一附属医院 一种制备视网膜色素上皮细胞的方法

Non-Patent Citations (13)

* Cited by examiner, † Cited by third party
Title
5965-5974: "Differential Effects of TGF and Vitreous on the Transformation of Retial Pigment Epithelial Cells.", 《INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE》 *
AMANDA-JAYNE F等: "Development of human embryonic stem cell therapies for age-related macular degeneration.", 《TRENDS IN NEUROSCIENCES》 *
D.E.BUCHHOLZ等: "Rapid and Efficient Directed Differentiation of Human Pluripotent Stem Cells Into Retinal Pigmented Epithelium.", 《STEM CELLS TRANSLATIONAL MEDICINE》 *
GAMM DAVID M等: "A novel serum-free method for culturing human prenatal retinal pigment epithelial cells.", 《INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE》 *
HUNG-CHI CHEN等: "Wnt signaling induces epithelial-mesenchymal transition with proliferation in ARPE-19 cells upon loss of contact inhibition.", 《LABORATORY INVESTIGATION》 *
J LIVERSIDGE等: "CD59 and CD48 expressed by rat retinal pigment epithelial cells are major ligands for the CD2-mediated alternative pathway of T cell acctivation.", 《THE JOURNAL OF IMMUNOLOGY》 *
JOSEPH REYNOLDS等: "Human embryonic stem cell applications for retinal degenerations.", 《EXPERIMENTAL EYE RESEARCH》 *
MARIA IDELSON等: "Directed Differentiation of Human Embryonic Stem Cells into Functional Retinal Pigment Epithelium Cells.", 《CELL STEM CELL》 *
MEYER JASON S等: "Modeling early retinal development with human embryonic and induced pluripotent stem cells.", 《PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES》 *
OSAKADA FUMITAKA等: "In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction.", 《JOURNAL OF CELL SCIENCE》 *
S.OKAMOTO等: "Induction of Retinal Pigment Epithelial Cells from Monkey iPS Cells.", 《INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE》 *
STUART M CHAMBERS等: "Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling.", 《NATURE BIOTECHNOLOGY》 *
YA FATOU NJIE-MBYE等: "Mechanism of action of hydrogen sulfide on cyclic AMP formation in rat retinal pigment epithelial cells.", 《EXPERIMENTAL EYE RESEARCH》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106344932A (zh) * 2016-08-26 2017-01-25 首都医科大学附属北京同仁医院 一种用于提高视网膜退行性疾病基因治疗效果的组合物及其应用
CN114450418A (zh) * 2019-07-19 2022-05-06 东京毅力科创株式会社 细胞的分化状态的评价方法
WO2023035608A1 (zh) * 2021-09-08 2023-03-16 湖南光琇高新生命科技有限公司 视网膜色素上皮细胞的制备方法
CN120905148A (zh) * 2025-10-11 2025-11-07 中国人民解放军陆军军医大学第一附属医院 一种基于ChR2和GCaMP5G双转染hESC构建起搏细胞制备视网膜类器官的方法

Also Published As

Publication number Publication date
CA2933083A1 (en) 2015-06-18
IL245594A0 (en) 2016-06-30
BR112016012129A8 (pt) 2020-05-12
JP2017504311A (ja) 2017-02-09
WO2015087231A1 (en) 2015-06-18
TW201538726A (zh) 2015-10-16
BR112016012129A2 (pt) 2017-08-08
AU2014363032A1 (en) 2016-06-09
TW201823451A (zh) 2018-07-01
AR098693A1 (es) 2016-06-08
KR20160095118A (ko) 2016-08-10
EP3080248A1 (en) 2016-10-19
KR101871084B1 (ko) 2018-06-25
US20150159134A1 (en) 2015-06-11
MX2016007671A (es) 2016-10-14
HK1221734A1 (zh) 2017-06-09

Similar Documents

Publication Publication Date Title
CN105814194A (zh) 产生视网膜色素上皮细胞的方法
US11560546B2 (en) Methods for neural conversion of human embryonic stem cells
JP7029144B2 (ja) 腺性下垂体又はその前駆組織の製造方法
JP5721111B2 (ja) 幹細胞の培地及び培養方法
AU2014352773B2 (en) Specification of functional cranial placode derivatives from human pluripotent stem cells
JP6995314B2 (ja) 網膜組織の製造法
EP2314671B1 (en) Method for culture of stem cell
JP5631210B2 (ja) 多能性幹細胞からのニューロン細胞の生成
AU2017214749B2 (en) Methods of differentiating stem cell-derived ectodermal lineage precursors
JP7737672B2 (ja) 下垂体ホルモン産生細胞及びその前駆細胞の分離法
EP3679125A1 (en) Methods of differentiating stem cell-derived ectodermal lineage precursors
KR20240093530A (ko) 뇌하수체 호르몬 생산 세포를 포함하는 세포 응집체 및 그 생산 방법
WO2024207102A1 (en) Compositions and methods for differentiating rpe cells

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1221734

Country of ref document: HK

WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20160727

WD01 Invention patent application deemed withdrawn after publication
REG Reference to a national code

Ref country code: HK

Ref legal event code: WD

Ref document number: 1221734

Country of ref document: HK