CA2904202C - Nanopore biosensors for detection of proteins and nucleic acids - Google Patents
Nanopore biosensors for detection of proteins and nucleic acids Download PDFInfo
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/255—Salmonella (G)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
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- Cell Biology (AREA)
- Electrochemistry (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Peptides Or Proteins (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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| US201361805068P | 2013-03-25 | 2013-03-25 | |
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| GB1313477.0 | 2013-07-29 | ||
| PCT/BE2014/000013 WO2014153625A1 (en) | 2013-03-25 | 2014-03-25 | Nanopore biosensors for detection of proteins and nucleic acids |
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| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| CN111534504B (zh) | 2014-01-22 | 2024-06-21 | 牛津纳米孔科技公开有限公司 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
| CN106459159B (zh) | 2014-05-02 | 2021-11-30 | 牛津纳米孔技术公司 | 突变孔 |
| US10400014B2 (en) | 2014-09-01 | 2019-09-03 | Oxford Nanopore Technologies Ltd. | Mutant CsgG pores |
| WO2016055778A1 (en) | 2014-10-07 | 2016-04-14 | Oxford Nanopore Technologies Limited | Mutant pores |
| CN107002130B (zh) * | 2014-11-11 | 2022-02-01 | 深圳华大基因研究院 | 多程测序 |
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| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
| CN107735686B (zh) * | 2015-04-14 | 2021-06-11 | 鲁汶天主教大学 | 具有内部蛋白质衔接子的纳米孔 |
| CN109072295B (zh) * | 2015-12-08 | 2022-05-31 | 鲁汶大学研究与发展中心 | 修饰的纳米孔，包含其的组合物及其应用 |
| US10975428B2 (en) | 2016-03-02 | 2021-04-13 | Oxford Nanopore Technologies Ltd. | Mutant pore |
| CN118326018B (zh) | 2016-04-06 | 2025-11-07 | 牛津纳米孔科技公开有限公司 | 突变体孔 |
| JP7027334B2 (ja) | 2016-04-21 | 2022-03-01 | エフ．ホフマン－ラ ロシュ アーゲー | アルファ溶血素バリアントおよびその使用 |
| FR3053119A1 (fr) * | 2016-06-24 | 2017-12-29 | Excilone | Procede de detection electrique de peptides, proteines et autres macromolecules |
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| CN119530363A (zh) | 2017-02-10 | 2025-02-28 | 牛津纳米孔科技公开有限公司 | 修饰的纳米孔、包含其的组合物及其用途 |
| GB201705764D0 (en) | 2017-04-10 | 2017-05-24 | Imp Innovations Ltd | Analyte detection method |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| CN117106037B (zh) | 2017-06-30 | 2025-07-25 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
| EP4265733A1 (en) | 2017-07-17 | 2023-10-25 | President and Fellows of Harvard College | Analysis of nanopore-matched protein shuttle for molecular characterization |
| EP3655423B1 (en) * | 2017-07-17 | 2023-12-20 | President and Fellows of Harvard College | Nanopore-matched protein shuttle for molecular characterization |
| EP3752284A4 (en) * | 2018-02-12 | 2022-05-25 | Oxford Nanopore Technologies plc | NANOPOROUS ARRANGEMENTS AND THEIR USES |
| GB201809207D0 (en) * | 2018-06-05 | 2018-07-25 | Univ Oxford Innovation Ltd | Molecular electronic device |
| CA3111488A1 (en) | 2018-09-11 | 2020-03-19 | Rijksuniversiteit Groningen | Biological nanopores having tunable pore diameters and uses thereof as analytical tools |
| US20200326325A1 (en) | 2019-04-12 | 2020-10-15 | Lisa Diamond | Nanosensor chip with compound nanopores |
| CN111912892B (zh) * | 2019-05-07 | 2022-03-25 | 南京大学 | 气单胞菌溶素纳米孔通道在生物磷酸化及相关酶分析中的应用 |
| CN112708544A (zh) * | 2019-10-25 | 2021-04-27 | 成都今是科技有限公司 | 基因测序的测量装置及其测量方法 |
| CN111235224B (zh) * | 2020-01-14 | 2023-06-20 | 广东工业大学 | 一种基于磁泳分离的生物分子精确修饰方法及装置 |
| CN112481363B (zh) * | 2020-03-09 | 2024-05-28 | 南京大学 | 突变Aerolysin单体在检测RNA碱基序列及RNA修饰方面的应用 |
| WO2021182957A1 (en) | 2020-03-11 | 2021-09-16 | Rijksuniversiteit Groningen | Engineered plyab nanopores and uses thereof. |
| US20230184767A1 (en) * | 2020-05-20 | 2023-06-15 | The Board Of Trustees Of The University Of Illinois | Methods and compositions for rapid direct detection and differentiation of infectious from noninfectious virus |
| KR102309424B1 (ko) * | 2020-09-28 | 2021-10-07 | 한국생명공학연구원 | 나노포어를 이용한 리보스위치-표적 물질의 스크리닝 방법 |
| GB202107192D0 (en) | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
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| IL320502A (en) | 2022-10-28 | 2025-06-01 | Univ Groningen | Nanopore-based protein efflux |
| GB202216162D0 (en) | 2022-10-31 | 2022-12-14 | Oxford Nanopore Tech Plc | Method |
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