AU2014245783B2 - Nanopore biosensors for detection of proteins and nucleic acids - Google Patents

Nanopore biosensors for detection of proteins and nucleic acids Download PDF

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Publication number
AU2014245783B2
AU2014245783B2 AU2014245783A AU2014245783A AU2014245783B2 AU 2014245783 B2 AU2014245783 B2 AU 2014245783B2 AU 2014245783 A AU2014245783 A AU 2014245783A AU 2014245783 A AU2014245783 A AU 2014245783A AU 2014245783 B2 AU2014245783 B2 AU 2014245783B2
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AU2014245783A1 (en
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Giovanni Maglia
Mikhael SOSKINE
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Katholieke Universiteit Leuven
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306Solid-phase reaction mechanisms
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/255Salmonella (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28Electrolytic cell components
    • G01N27/30Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3278Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721Investigating individual macromolecules, e.g. by translocation through nanopores

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Electrochemistry (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2014245783A 2013-03-25 2014-03-25 Nanopore biosensors for detection of proteins and nucleic acids Active AU2014245783B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361805068P 2013-03-25 2013-03-25
US61/805,068 2013-03-25
GB1313477.0 2013-07-29
GBGB1313477.0A GB201313477D0 (en) 2013-07-29 2013-07-29 Nanopore biosensors for detection of proteins and nucleic acids
PCT/BE2014/000013 WO2014153625A1 (en) 2013-03-25 2014-03-25 Nanopore biosensors for detection of proteins and nucleic acids

Publications (2)

Publication Number Publication Date
AU2014245783A1 AU2014245783A1 (en) 2015-10-01
AU2014245783B2 true AU2014245783B2 (en) 2018-08-16

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AU2014245783A Active AU2014245783B2 (en) 2013-03-25 2014-03-25 Nanopore biosensors for detection of proteins and nucleic acids

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US (7) US10006905B2 (OSRAM)
EP (1) EP2978773B1 (OSRAM)
JP (1) JP6592427B2 (OSRAM)
CN (1) CN105358567B (OSRAM)
AU (1) AU2014245783B2 (OSRAM)
BR (1) BR112015024452B1 (OSRAM)
CA (1) CA2904202C (OSRAM)
GB (1) GB201313477D0 (OSRAM)
WO (1) WO2014153625A1 (OSRAM)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
US10385389B2 (en) 2014-01-22 2019-08-20 Oxford Nanopore Technologies Ltd. Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
WO2015166275A1 (en) 2014-05-02 2015-11-05 Oxford Nanopore Technologies Limited Mutant pores
KR20170042794A (ko) 2014-09-01 2017-04-19 브이아이비 브이지더블유 돌연변이체 csgg 포어
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
DK3218519T3 (da) * 2014-11-11 2020-12-21 Bgi Shenzhen Multipass-sekvensering
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) * 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
US11169138B2 (en) * 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
US10976300B2 (en) 2015-12-08 2021-04-13 Katholieke Universiteit Leuven Modified nanopores, compositions comprising the same, and uses thereof
CN116200476A (zh) 2016-03-02 2023-06-02 牛津纳米孔科技公开有限公司 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔
EP3440098B1 (en) 2016-04-06 2022-07-27 Oxford Nanopore Technologies plc Mutant pore
US10351908B2 (en) * 2016-04-21 2019-07-16 Roche Sequencing Solutions, Inc. Alpha-hemolysin variants and uses thereof
FR3053119A1 (fr) * 2016-06-24 2017-12-29 Excilone Procede de detection electrique de peptides, proteines et autres macromolecules
GB201612458D0 (en) * 2016-07-14 2016-08-31 Howorka Stefan And Pugh Genevieve Membrane spanning DNA nanopores for molecular transport
WO2018039587A1 (en) * 2016-08-26 2018-03-01 Ohio State Innovation Foundation Phi29 nanochannel for early detection of breast cancer biomarkers
CN119530363A (zh) 2017-02-10 2025-02-28 牛津纳米孔科技公开有限公司 修饰的纳米孔、包含其的组合物及其用途
GB201705764D0 (en) 2017-04-10 2017-05-24 Imp Innovations Ltd Analyte detection method
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN110914290A (zh) 2017-06-30 2020-03-24 弗拉芒区生物技术研究所 新颖蛋白孔
CN110914291B (zh) * 2017-07-17 2024-10-29 哈佛大学校董委员会 用于分子表征的纳米孔匹配的蛋白质穿梭物
US11639922B2 (en) 2017-07-17 2023-05-02 President And Fellows Of Harvard College Nanopore-matched protein shuttle for molecular characterization and methodology for data analysis thereof
AU2019218263B2 (en) * 2018-02-12 2024-12-12 Oxford Nanopore Technologies Plc Nanopore assemblies and uses thereof
GB201809207D0 (en) * 2018-06-05 2018-07-25 Univ Oxford Innovation Ltd Molecular electronic device
BR112021004675A2 (pt) 2018-09-11 2021-06-01 Rijksuniversiteit Groningen nanoporos biológicos com diâmetros de poros ajustáveis e usos dos mesmos como ferramentas analíticas
US20200326325A1 (en) 2019-04-12 2020-10-15 Lisa Diamond Nanosensor chip with compound nanopores
CN111912892B (zh) * 2019-05-07 2022-03-25 南京大学 气单胞菌溶素纳米孔通道在生物磷酸化及相关酶分析中的应用
CN112708544A (zh) * 2019-10-25 2021-04-27 成都今是科技有限公司 基因测序的测量装置及其测量方法
CN111235224B (zh) * 2020-01-14 2023-06-20 广东工业大学 一种基于磁泳分离的生物分子精确修饰方法及装置
CN112481363B (zh) * 2020-03-09 2024-05-28 南京大学 突变Aerolysin单体在检测RNA碱基序列及RNA修饰方面的应用
US20230176034A1 (en) 2020-03-11 2023-06-08 Rijksuniversiteit Groningen Engineered PlyAB Nanopores and Uses Thereof
WO2021236828A1 (en) * 2020-05-20 2021-11-25 The Board Of Trustees Of The University Of Illinois Methods and compositions for rapid direct detection and differentiation of infectious from noninfectious virus
KR102309424B1 (ko) * 2020-09-28 2021-10-07 한국생명공학연구원 나노포어를 이용한 리보스위치-표적 물질의 스크리닝 방법
GB202107192D0 (en) 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
GB202118908D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202118906D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
IL320502A (en) 2022-10-28 2025-06-01 Univ Groningen Nanopore-based protein efflux
GB202216162D0 (en) 2022-10-31 2022-12-14 Oxford Nanopore Tech Plc Method
GB202307486D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Plc Method
GB202317028D0 (en) 2023-11-06 2023-12-20 Oxford Nanopore Tech Ltd Method

Family Cites Families (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI82266C (fi) 1982-10-19 1991-02-11 Cetus Corp Foerfarande foer framstaellning av il-2 -mutein.
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5777078A (en) 1993-04-28 1998-07-07 Worcester Foundation For Experimental Biology Triggered pore-forming agents
CA2160909A1 (en) 1993-04-28 1994-11-10 Hagan Bayley Cell-targeted lytic pore-forming agents
DE4320201A1 (de) 1993-06-18 1995-01-12 Asta Medica Ag Verwendung von Cetrorelix und weiteren Nona- und Dekapeptiden zur Herstellung eines Arzneimittels zur Bekämpfung von Aids und zur Wachstumsstimulation
US5386373A (en) 1993-08-05 1995-01-31 Pavilion Technologies, Inc. Virtual continuous emission monitoring system with sensor validation
US5561043A (en) 1994-01-31 1996-10-01 Trustees Of Boston University Self-assembling multimeric nucleic acid constructs
US7569341B2 (en) 1994-01-31 2009-08-04 Trustees Of Boston University Nucleic acid directed immobilization arrays and methods of assembly
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
JP3891620B2 (ja) 1996-11-14 2007-03-14 株式会社アイシン・コスモス研究所 ヘアピン型構造の核酸プローブ分子、及び該核酸プローブ分子を利用した核酸検出方法
US20020197614A1 (en) 1997-05-16 2002-12-26 Mosaic Technologies, Inc. Electrophoretic analysis of target molecules using adapter molecules
WO1999005167A1 (en) 1997-07-25 1999-02-04 University Of Massachusetts Designed protein pores as components for biosensors
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6127166A (en) 1997-11-03 2000-10-03 Bayley; Hagan Molluscan ligament polypeptides and genes encoding them
JPH11137260A (ja) 1997-11-06 1999-05-25 Soyaku Gijutsu Kenkyusho:Kk 抗インフルエンザウイルス環状ダンベル型rna−dnaキメラ化合物及び抗インフルエンザウイルス剤
US6123819A (en) 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
DE19826758C1 (de) 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US7155344B1 (en) 1998-07-27 2006-12-26 Caliper Life Sciences, Inc. Distributed database for analytical instruments
US7588900B2 (en) 2001-07-03 2009-09-15 The Regents Of The University Of California Mammalian sweet and amino acid heterodimeric taste receptors
US6150112A (en) 1998-09-18 2000-11-21 Yale University Methods for identifying DNA sequences for use in comparison of DNA samples by their lack of polymorphism using Y shape adaptors
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
EP1192103A1 (en) 1999-06-22 2002-04-03 President And Fellows of Harvard College Control of solid state dimensional features
AU5668100A (en) 1999-06-29 2001-01-22 University Health Network Peptide conjugates for the stabilization of membrane proteins and interactions with biological membranes
EP1208207A2 (de) 1999-08-31 2002-05-29 Michael Niederweis Verfahren zur herstellung eines kanalbildenden proteins
JP2003532380A (ja) 1999-12-02 2003-11-05 モレキュラー ステージング,インコーポレイテッド 線状自己アニーリングセグメントからの一本鎖環状dnaの産出
US6916665B2 (en) * 2000-02-11 2005-07-12 The Texas A&M University System Biosensor compositions and methods of use
WO2001062943A1 (en) 2000-02-25 2001-08-30 Invitrogen Corporation Topoisomerase linker-mediated amplification methods
US20020132978A1 (en) 2000-03-21 2002-09-19 Hans-Peter Gerber VEGF-modulated genes and methods employing them
JP2003527849A (ja) 2000-03-22 2003-09-24 キュラゲン コーポレイション 新規ペプチド及びこれをコードする核酸
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
ATE369697T1 (de) 2000-09-25 2007-08-15 Sensovation Ag Vorrichtung und verfahren zur optischen messung
US6936433B2 (en) 2000-11-27 2005-08-30 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US20030087232A1 (en) 2001-01-25 2003-05-08 Fred Christians Methods for screening polypeptides
US7807408B2 (en) 2001-03-19 2010-10-05 President & Fellows Of Harvard College Directed evolution of proteins
JP2002355035A (ja) 2001-05-08 2002-12-10 Inst Of Physical & Chemical Res スフィンゴミエリン検出プローブ
US6995236B2 (en) 2001-05-08 2006-02-07 Riken Sphingomyelin detecting probe
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
CA2450435C (en) 2001-08-31 2009-02-03 Gentex Corporation Vehicle lamp assembly with heat sink
CA2518626A1 (en) 2002-03-15 2003-09-25 Nuevolution A/S An improved method for synthesising templated molecules
AU2003245272A1 (en) 2002-05-10 2003-11-11 The Texas A And M University System Stochastic sensing through covalent interactions
US20040209299A1 (en) 2003-03-07 2004-10-21 Rubicon Genomics, Inc. In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA
JP4722035B2 (ja) 2003-03-25 2011-07-13 アジレント・テクノロジーズ・インク Dnaポリメラーゼ融合物およびその使用
US7163658B2 (en) 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
US7344882B2 (en) 2003-05-12 2008-03-18 Bristol-Myers Squibb Company Polynucleotides encoding variants of the TRP channel family member, LTRPC3
AU2003904237A0 (en) 2003-08-08 2003-08-21 Garvan Institute Of Medical Research Novel translocation assay
WO2005056750A2 (en) 2003-12-11 2005-06-23 Quark Biotech, Inc. Inversion-duplication of nucleic acids and libraries prepared thereby
EP1721283B1 (en) 2004-02-06 2022-11-30 Council of Scientific and Industrial Research Computational method for identifying adhesin and adhesin-like proteins of therapeutic potential
JP2005253427A (ja) 2004-03-15 2005-09-22 Aisin Seiki Co Ltd 核酸検出方法及び核酸単離方法
US7238485B2 (en) 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
AU2005272823B2 (en) 2004-08-13 2012-04-12 President And Fellows Of Harvard College An ultra high-throughput opti-nanopore DNA readout platform
US20060105461A1 (en) 2004-10-22 2006-05-18 May Tom-Moy Nanopore analysis system
EP1842061A4 (en) 2004-12-21 2009-05-13 Texas A & M Univ Sys HIGH-TEMPERATURE ION CHANNELS AND ION PORES
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US7272518B2 (en) 2005-07-01 2007-09-18 Square D Company Automated hierarchy classification in utility monitoring systems
WO2007024997A2 (en) 2005-08-22 2007-03-01 Fermalogic, Inc. Methods of increasing production of secondary metabolites
US7877154B2 (en) 2005-09-30 2011-01-25 Fisher-Rosemount Systems, Inc. Method and system for controlling a batch process
KR100730350B1 (ko) 2005-10-17 2007-06-19 삼성전자주식회사 표면처리된 나노포어를 이용한 dna 검출방법 및검출장치
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
ATE518010T1 (de) 2005-12-22 2011-08-15 Pacific Biosciences California Aktive oberflächengekoppelte polymerasen
US7932029B1 (en) 2006-01-04 2011-04-26 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
US7849581B2 (en) 2006-05-05 2010-12-14 University Of Utah Research Foundation Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof
CN101578373A (zh) 2006-09-06 2009-11-11 费斯生物制药公司 融合肽治疗组合物
US7638034B2 (en) 2006-09-21 2009-12-29 Los Alamos National Security, Llc Electrochemical detection of single molecules using abiotic nanopores having electrically tunable dimensions
WO2008045575A2 (en) 2006-10-13 2008-04-17 J. Craig Venter Institute, Inc. Sequencing method
US8594848B2 (en) 2006-11-28 2013-11-26 Lester F. Ludwig Reconfigurable chemical process systems
AU2008217579A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Formation of lipid bilayers
US20110005918A1 (en) 2007-04-04 2011-01-13 Akeson Mark A Compositions, devices, systems, and methods for using a nanopore
WO2008147816A2 (en) * 2007-05-22 2008-12-04 Cornell Research Foundation, Inc. Compositions and methods for the display of proteins on the surface of bacteria and their derived vesicles and uses thereof
US8158344B2 (en) 2007-06-29 2012-04-17 Duke University Methods and compositions for correlating genetic markers with multiple sclerosis
GB0716005D0 (en) 2007-08-16 2007-09-26 Imp Innovations Ltd Single molecule spectroscopy using nanoporpus membranes
GB0716264D0 (en) 2007-08-21 2007-09-26 Isis Innovation Bilayers
EP3540436B1 (en) 2007-09-12 2023-11-01 President And Fellows Of Harvard College High-resolution molecular sensor
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
WO2009052214A2 (en) 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
EP2232233B1 (en) 2007-12-31 2014-03-12 Fujirebio Inc. Clusters of microresonators for cavity mode optical sensing
US8231969B2 (en) 2008-03-26 2012-07-31 University Of Utah Research Foundation Asymmetrically functionalized nanoparticles
CA2719747C (en) 2008-03-28 2018-02-20 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US8772041B2 (en) 2008-05-22 2014-07-08 The Regents Of The University Of California Membrane precursors and membranes formed therefrom
US8652771B2 (en) 2008-05-28 2014-02-18 University of Souther California Measurement of succinate in urine samples as a biomarker of kidney damage in diabetic subjects
CN102245760A (zh) 2008-07-07 2011-11-16 牛津纳米孔技术有限公司 酶-孔构建体
CN102144037A (zh) 2008-07-07 2011-08-03 牛津纳米孔技术有限公司 检测碱基的孔
WO2010034018A2 (en) 2008-09-22 2010-03-25 University Of Washington Msp nanopores and related methods
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
US20110246084A1 (en) 2008-11-26 2011-10-06 Mostafa Ronaghi Methods and systems for analysis of sequencing data
WO2010086622A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
US9222082B2 (en) 2009-01-30 2015-12-29 Oxford Nanopore Technologies Limited Hybridization linkers
EP2398494A4 (en) 2009-02-23 2015-10-28 Cytomx Therapeutics Inc Proproteins and their methods of use
FR2943656A1 (fr) 2009-03-25 2010-10-01 Air Liquide Procede et installation de production d'hydrogene mettant en oeuvre un compresseur thermocinetique
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
JP5781502B2 (ja) 2009-04-20 2015-09-24 オックスフォード ナノポール テクノロジーズ リミテッド 脂質二重層センサアレイ
CN102741430B (zh) 2009-12-01 2016-07-13 牛津楠路珀尔科技有限公司 生化分析仪器、用于进行生化分析的第一模块以及相关方法
FR2955773B1 (fr) 2010-02-01 2017-05-26 Commissariat Energie Atomique Complexe moleculaire de ciblage des antigenes vers les cellules presentatrices d'antigene et ses applications pour la vaccination
CN102834527B (zh) 2010-02-23 2015-06-03 华盛顿大学 用纳米孔的分析物测序
EP2556085A2 (en) 2010-04-05 2013-02-13 Bar-Ilan University Protease-activatable pore-forming polypeptides
EP2622343B1 (en) 2010-10-01 2016-01-20 Oxford Nanopore Technologies Limited Biochemical analysis apparatus using nanopores
CN102116783B (zh) 2010-12-31 2013-05-29 北京普源精电科技有限公司 一种波形显示方法
GB201100516D0 (en) 2011-01-12 2011-02-23 Isis Innovation Method using fluorinated amphiphiles
CN102174554A (zh) 2011-01-24 2011-09-07 内蒙古民族大学 双控双调节原核表达载体系统及其构建方法和用途
JP6169976B2 (ja) 2011-02-11 2017-07-26 オックスフォード ナノポール テクノロジーズ リミテッド 変異体細孔
AU2012264497B2 (en) 2011-05-27 2017-06-15 Oxford Nanopore Technologies Limited Coupling method
WO2012166906A1 (en) 2011-05-31 2012-12-06 Massachusetts Institute Of Technology Cell-directed synthesis of multifunctional nanopatterns and nanomaterials
WO2013014451A1 (en) 2011-07-25 2013-01-31 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
CA3113287C (en) 2011-09-23 2022-12-20 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
WO2013109970A1 (en) 2012-01-20 2013-07-25 Genia Technologies, Inc. Nanopore based molecular detection and sequencing
US10739341B2 (en) * 2012-02-15 2020-08-11 Oxford Nanopore Technologies Limited Aptamer method
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
TWI655213B (zh) 2012-07-13 2019-04-01 目立康股份有限公司 自我組織化肽衍生物的製造方法
EP2875152B1 (en) 2012-07-19 2019-10-09 Oxford Nanopore Technologies Limited Enzyme construct
JP6614972B2 (ja) 2012-07-19 2019-12-04 オックスフォード ナノポール テクノロジーズ リミテッド 修飾ヘリカーゼ
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
JP6375301B2 (ja) 2012-10-26 2018-08-15 オックスフォード ナノポール テクノロジーズ リミテッド 液滴界面
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US20140206842A1 (en) 2013-01-22 2014-07-24 Muhammed Majeed Peptides Modified with Triterpenoids and Small Organic Molecules: Synthesis and use in Cosmeceutical
CN105074458B (zh) 2013-02-07 2019-06-28 耶路撒冷希伯来大学伊森姆研究发展有限公司 杂化纳米孔及其用于检测分析物的用途
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
SG11201507138RA (en) 2013-03-08 2015-10-29 Oxford Nanopore Tech Ltd Enzyme stalling method
EP3919617A1 (en) 2013-03-13 2021-12-08 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2014153047A1 (en) 2013-03-14 2014-09-25 The Trustees Of Boston University Optoelectronic control of solid-state nanopores
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
ES2817425T3 (es) 2013-05-24 2021-04-07 Illumina Cambridge Ltd Secuenciado pirofosforolítico usando nanoporos
WO2015051378A1 (en) 2013-10-04 2015-04-09 University Of Washington Through Its Center For Commercialization Systems and methods for nanopore-based analysis of nucleic acids
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
US10724018B2 (en) 2013-10-18 2020-07-28 Oxford Nanopore Technologies Ltd. Modified helicases
CN105934522B (zh) 2013-11-26 2021-07-20 伊鲁米那股份有限公司 用于多核苷酸测序的组合物和方法
US20160326220A1 (en) 2013-12-24 2016-11-10 Vib Vzw Secretion and functional display of chimeric polypeptides
US10385389B2 (en) 2014-01-22 2019-08-20 Oxford Nanopore Technologies Ltd. Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
CN106460061B (zh) 2014-04-04 2020-03-06 牛津纳米孔技术公司 在双链核酸分子的两端使用纳米孔和锚分子来表征所述双链核酸的方法
WO2015166275A1 (en) 2014-05-02 2015-11-05 Oxford Nanopore Technologies Limited Mutant pores
FR3023394B1 (fr) 2014-07-02 2017-12-29 Adn Access Data Networks Dispositif permettant de faciliter l'enseignement de la langue amharique et d'en normaliser l'ecriture
KR20170042794A (ko) 2014-09-01 2017-04-19 브이아이비 브이지더블유 돌연변이체 csgg 포어
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
US10976300B2 (en) 2015-12-08 2021-04-13 Katholieke Universiteit Leuven Modified nanopores, compositions comprising the same, and uses thereof
CN116200476A (zh) 2016-03-02 2023-06-02 牛津纳米孔科技公开有限公司 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔
EP3440098B1 (en) 2016-04-06 2022-07-27 Oxford Nanopore Technologies plc Mutant pore
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN110914290A (zh) 2017-06-30 2020-03-24 弗拉芒区生物技术研究所 新颖蛋白孔

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
A. ATKINS, "Structure-Function Relationships of a Novel Bacterial Toxin, Hemolysin E. THE ROLE OF alpha G", JOURNAL OF BIOLOGICAL CHEMISTRY, (20001222), vol. 275, no. 52, doi:10.1074/jbc.M005420200, ISSN 0021-9258, pages 41150 - 41155 *

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