JP6592427B2 - タンパク質および核酸を検出するためのナノ細孔バイオセンサー - Google Patents
タンパク質および核酸を検出するためのナノ細孔バイオセンサー Download PDFInfo
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- JP6592427B2 JP6592427B2 JP2016504425A JP2016504425A JP6592427B2 JP 6592427 B2 JP6592427 B2 JP 6592427B2 JP 2016504425 A JP2016504425 A JP 2016504425A JP 2016504425 A JP2016504425 A JP 2016504425A JP 6592427 B2 JP6592427 B2 JP 6592427B2
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/255—Salmonella (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Pathology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Nanotechnology (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Electrochemistry (AREA)
- Cell Biology (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Peptides Or Proteins (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361805068P | 2013-03-25 | 2013-03-25 | |
| US61/805,068 | 2013-03-25 | ||
| GBGB1313477.0A GB201313477D0 (en) | 2013-07-29 | 2013-07-29 | Nanopore biosensors for detection of proteins and nucleic acids |
| GB1313477.0 | 2013-07-29 | ||
| PCT/BE2014/000013 WO2014153625A1 (en) | 2013-03-25 | 2014-03-25 | Nanopore biosensors for detection of proteins and nucleic acids |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016516735A JP2016516735A (ja) | 2016-06-09 |
| JP2016516735A5 JP2016516735A5 (OSRAM) | 2017-04-27 |
| JP6592427B2 true JP6592427B2 (ja) | 2019-10-16 |
Family
ID=49167071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016504425A Active JP6592427B2 (ja) | 2013-03-25 | 2014-03-25 | タンパク質および核酸を検出するためのナノ細孔バイオセンサー |
Country Status (9)
| Country | Link |
|---|---|
| US (7) | US10006905B2 (OSRAM) |
| EP (1) | EP2978773B1 (OSRAM) |
| JP (1) | JP6592427B2 (OSRAM) |
| CN (1) | CN105358567B (OSRAM) |
| AU (1) | AU2014245783B2 (OSRAM) |
| BR (1) | BR112015024452B1 (OSRAM) |
| CA (1) | CA2904202C (OSRAM) |
| GB (1) | GB201313477D0 (OSRAM) |
| WO (1) | WO2014153625A1 (OSRAM) |
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| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| WO2013153359A1 (en) | 2012-04-10 | 2013-10-17 | Oxford Nanopore Technologies Limited | Mutant lysenin pores |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| CN106103741B (zh) | 2014-01-22 | 2020-03-13 | 牛津纳米孔技术公司 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
| WO2015166276A1 (en) | 2014-05-02 | 2015-11-05 | Oxford Nanopore Technologies Limited | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
| EP4053150A1 (en) | 2014-09-01 | 2022-09-07 | Vib Vzw | Mutant csgg pores |
| US10266885B2 (en) | 2014-10-07 | 2019-04-23 | Oxford Nanopore Technologies Ltd. | Mutant pores |
| CN107002130B (zh) * | 2014-11-11 | 2022-02-01 | 深圳华大基因研究院 | 多程测序 |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
| EP3283887B1 (en) * | 2015-04-14 | 2021-07-21 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| CN109072295B (zh) * | 2015-12-08 | 2022-05-31 | 鲁汶大学研究与发展中心 | 修饰的纳米孔，包含其的组合物及其应用 |
| EP3423486B1 (en) | 2016-03-02 | 2021-12-29 | Oxford Nanopore Technologies PLC | Mutant pore |
| CA3020203A1 (en) | 2016-04-06 | 2017-10-12 | Oxford Nanopore Technologies Limited | Mutant pore |
| EP3445775A1 (en) | 2016-04-21 | 2019-02-27 | H. Hoffnabb-La Roche Ag | Alpha-hemolysin variants and uses thereof |
| FR3053119A1 (fr) * | 2016-06-24 | 2017-12-29 | Excilone | Procede de detection electrique de peptides, proteines et autres macromolecules |
| GB201612458D0 (en) * | 2016-07-14 | 2016-08-31 | Howorka Stefan And Pugh Genevieve | Membrane spanning DNA nanopores for molecular transport |
| WO2018039587A1 (en) * | 2016-08-26 | 2018-03-01 | Ohio State Innovation Foundation | Phi29 nanochannel for early detection of breast cancer biomarkers |
| US11840556B2 (en) | 2017-02-10 | 2023-12-12 | Oxford Nanopore Technologies Plc | Modified nanopores, compositions comprising the same, and uses thereof |
| GB201705764D0 (en) | 2017-04-10 | 2017-05-24 | Imp Innovations Ltd | Analyte detection method |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| CN117106037B (zh) | 2017-06-30 | 2025-07-25 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
| EP3655543B1 (en) | 2017-07-17 | 2023-09-06 | President and Fellows of Harvard College | Analysis of nanopore-matched protein shuttle for molecular characterization |
| EP3655423B1 (en) * | 2017-07-17 | 2023-12-20 | President and Fellows of Harvard College | Nanopore-matched protein shuttle for molecular characterization |
| AU2019218263B2 (en) * | 2018-02-12 | 2024-12-12 | Oxford Nanopore Technologies Plc | Nanopore assemblies and uses thereof |
| GB201809207D0 (en) * | 2018-06-05 | 2018-07-25 | Univ Oxford Innovation Ltd | Molecular electronic device |
| WO2020055246A1 (en) | 2018-09-11 | 2020-03-19 | Rijksuniversiteit Groningen | Biological nanopores having tunable pore diameters and uses thereof as analytical tools |
| US20200326325A1 (en) | 2019-04-12 | 2020-10-15 | Lisa Diamond | Nanosensor chip with compound nanopores |
| CN111912892B (zh) * | 2019-05-07 | 2022-03-25 | 南京大学 | 气单胞菌溶素纳米孔通道在生物磷酸化及相关酶分析中的应用 |
| CN112708544A (zh) * | 2019-10-25 | 2021-04-27 | 成都今是科技有限公司 | 基因测序的测量装置及其测量方法 |
| CN111235224B (zh) * | 2020-01-14 | 2023-06-20 | 广东工业大学 | 一种基于磁泳分离的生物分子精确修饰方法及装置 |
| CN112481363B (zh) * | 2020-03-09 | 2024-05-28 | 南京大学 | 突变Aerolysin单体在检测RNA碱基序列及RNA修饰方面的应用 |
| US20230176034A1 (en) | 2020-03-11 | 2023-06-08 | Rijksuniversiteit Groningen | Engineered PlyAB Nanopores and Uses Thereof |
| US20230184767A1 (en) * | 2020-05-20 | 2023-06-15 | The Board Of Trustees Of The University Of Illinois | Methods and compositions for rapid direct detection and differentiation of infectious from noninfectious virus |
| KR102309424B1 (ko) * | 2020-09-28 | 2021-10-07 | 한국생명공학연구원 | 나노포어를 이용한 리보스위치-표적 물질의 스크리닝 방법 |
| GB202107192D0 (en) | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
| GB202118908D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202118906D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| EP4609204A1 (en) | 2022-10-28 | 2025-09-03 | Rijksuniversiteit Groningen | Nanopore-based analysis of proteins |
| GB202216162D0 (en) | 2022-10-31 | 2022-12-14 | Oxford Nanopore Tech Plc | Method |
| GB202307486D0 (en) | 2023-05-18 | 2023-07-05 | Oxford Nanopore Tech Plc | Method |
| GB202317028D0 (en) | 2023-11-06 | 2023-12-20 | Oxford Nanopore Tech Ltd | Method |
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| CN105358567B (zh) | 2021-12-10 |
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