CN107002130B - 多程测序 - Google Patents
多程测序 Download PDFInfo
- Publication number
- CN107002130B CN107002130B CN201580061261.7A CN201580061261A CN107002130B CN 107002130 B CN107002130 B CN 107002130B CN 201580061261 A CN201580061261 A CN 201580061261A CN 107002130 B CN107002130 B CN 107002130B
- Authority
- CN
- China
- Prior art keywords
- sequence
- target sequence
- concatemer
- target
- copies
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
Abstract
Description
Claims (28)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462078306P | 2014-11-11 | 2014-11-11 | |
US62/078,306 | 2014-11-11 | ||
PCT/US2015/059903 WO2016077313A1 (en) | 2014-11-11 | 2015-11-10 | Multi-pass sequencing |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107002130A CN107002130A (zh) | 2017-08-01 |
CN107002130B true CN107002130B (zh) | 2022-02-01 |
Family
ID=55954931
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201580061261.7A Active CN107002130B (zh) | 2014-11-11 | 2015-11-10 | 多程测序 |
Country Status (5)
Country | Link |
---|---|
US (1) | US10626455B2 (zh) |
EP (1) | EP3218519B1 (zh) |
CN (1) | CN107002130B (zh) |
DK (1) | DK3218519T3 (zh) |
WO (1) | WO2016077313A1 (zh) |
Families Citing this family (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11111544B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
US9424392B2 (en) | 2005-11-26 | 2016-08-23 | Natera, Inc. | System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals |
US11111543B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
US8825412B2 (en) | 2010-05-18 | 2014-09-02 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
US20190010543A1 (en) | 2010-05-18 | 2019-01-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
US11408031B2 (en) | 2010-05-18 | 2022-08-09 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
US11326208B2 (en) | 2010-05-18 | 2022-05-10 | Natera, Inc. | Methods for nested PCR amplification of cell-free DNA |
US11939634B2 (en) | 2010-05-18 | 2024-03-26 | Natera, Inc. | Methods for simultaneous amplification of target loci |
US11332785B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
US10316362B2 (en) | 2010-05-18 | 2019-06-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
US9677118B2 (en) | 2014-04-21 | 2017-06-13 | Natera, Inc. | Methods for simultaneous amplification of target loci |
US11332793B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for simultaneous amplification of target loci |
US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
US11339429B2 (en) | 2010-05-18 | 2022-05-24 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
EP2656263B1 (en) | 2010-12-22 | 2019-11-06 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
RU2717641C2 (ru) | 2014-04-21 | 2020-03-24 | Натера, Инк. | Обнаружение мутаций и плоидности в хромосомных сегментах |
US11479812B2 (en) | 2015-05-11 | 2022-10-25 | Natera, Inc. | Methods and compositions for determining ploidy |
JP6867045B2 (ja) * | 2015-08-12 | 2021-04-28 | ザ チャイニーズ ユニバーシティ オブ ホンコン | 血漿dnaの単分子配列決定 |
US11485996B2 (en) | 2016-10-04 | 2022-11-01 | Natera, Inc. | Methods for characterizing copy number variation using proximity-litigation sequencing |
US10011870B2 (en) | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
WO2018211497A1 (en) * | 2017-05-14 | 2018-11-22 | Foresee Genomic Ltd | Dna construct for sequencing and method for preparing the same |
SG11202001858PA (en) * | 2017-10-09 | 2020-04-29 | Psomagen Inc | Single molecule sequencing and unique molecular identifiers to characterize nucleic acid sequences |
US20210079461A1 (en) * | 2018-04-20 | 2021-03-18 | The Regents Of The University Of California | Nucleic Acid Sequencing Methods and Computer-Readable Media for Practicing Same |
US11525159B2 (en) | 2018-07-03 | 2022-12-13 | Natera, Inc. | Methods for detection of donor-derived cell-free DNA |
WO2020113581A1 (zh) * | 2018-12-07 | 2020-06-11 | 深圳华大生命科学研究院 | 纳米孔测序方法 |
CN109852678A (zh) * | 2019-03-01 | 2019-06-07 | 天津大学 | 基于碳纳米管和单链dna缠绕结构的dna测序方法 |
CN113646471A (zh) * | 2019-09-20 | 2021-11-12 | 武汉华大医学检验所有限公司 | 测序文库的构建方法、测序方法及试剂盒和应用 |
CN113257351A (zh) * | 2020-02-12 | 2021-08-13 | 赛纳生物科技(北京)有限公司 | 一种用于多碱基基因测序的基因文库及其构建方法 |
CA3170856A1 (en) * | 2020-02-13 | 2021-08-19 | Garvan Institute Of Medical Research | Reference ladders and adaptors |
AU2021230282A1 (en) | 2020-03-03 | 2022-09-22 | Pacific Biosciences Of California, Inc. | Methods and compositions for sequencing double stranded nucleic acids |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1225234A2 (en) * | 2001-01-20 | 2002-07-24 | Agilent Technologies, Inc. (a Delaware corporation) | Synthesis of nucleic acid |
WO2006095169A1 (en) * | 2005-03-08 | 2006-09-14 | Lingvitae As | Method for preparing polynucleotides for analysis |
US8153375B2 (en) * | 2008-03-28 | 2012-04-10 | Pacific Biosciences Of California, Inc. | Compositions and methods for nucleic acid sequencing |
WO2013185137A1 (en) * | 2012-06-08 | 2013-12-12 | Pacific Biosciences Of California, Inc. | Modified base detection with nanopore sequencing |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2183388A4 (en) | 2007-07-26 | 2010-09-08 | Pacific Biosciences California | SEQUENCING MOLECULAR REDUNDANT |
WO2010117470A2 (en) * | 2009-04-10 | 2010-10-14 | Pacific Biosciences Of California, Inc. | Nanopore sequencing devices and methods |
WO2012135658A2 (en) | 2011-03-30 | 2012-10-04 | Noblegen Biosciences, Inc. | Sequence preserved dna conversion for optical nanopore sequencing |
JP6298404B2 (ja) | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
US20130040827A1 (en) * | 2011-08-14 | 2013-02-14 | Stephen C. Macevicz | Method and compositions for detecting and sequencing nucleic acids |
US10152569B2 (en) | 2011-09-26 | 2018-12-11 | Gen-Probe Incorporated | Algorithms for sequence determinations |
US20130296173A1 (en) * | 2012-04-23 | 2013-11-07 | Complete Genomics, Inc. | Pre-anchor wash |
JP2014029594A (ja) * | 2012-07-31 | 2014-02-13 | Canon Inc | 情報端末及びその制御方法、並びにプログラム |
GB201313477D0 (en) * | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
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2015
- 2015-11-10 EP EP15858699.0A patent/EP3218519B1/en active Active
- 2015-11-10 CN CN201580061261.7A patent/CN107002130B/zh active Active
- 2015-11-10 US US15/525,253 patent/US10626455B2/en active Active
- 2015-11-10 WO PCT/US2015/059903 patent/WO2016077313A1/en active Application Filing
- 2015-11-10 DK DK15858699.0T patent/DK3218519T3/da active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1225234A2 (en) * | 2001-01-20 | 2002-07-24 | Agilent Technologies, Inc. (a Delaware corporation) | Synthesis of nucleic acid |
WO2006095169A1 (en) * | 2005-03-08 | 2006-09-14 | Lingvitae As | Method for preparing polynucleotides for analysis |
US8153375B2 (en) * | 2008-03-28 | 2012-04-10 | Pacific Biosciences Of California, Inc. | Compositions and methods for nucleic acid sequencing |
WO2013185137A1 (en) * | 2012-06-08 | 2013-12-12 | Pacific Biosciences Of California, Inc. | Modified base detection with nanopore sequencing |
Non-Patent Citations (2)
Title |
---|
High-throughput DNA sequencing errors are reduced by orders of magnitude using circle sequencing;Dianne I.Lou等;《PNAs》;20131203;第110卷(第49期);第19872-19877页 * |
第三代测序技术及其应用;张得芳 等;《中国生物工程杂志》;20130515;第33卷(第5期);第125-131页 * |
Also Published As
Publication number | Publication date |
---|---|
US10626455B2 (en) | 2020-04-21 |
EP3218519A4 (en) | 2018-07-25 |
EP3218519A1 (en) | 2017-09-20 |
WO2016077313A1 (en) | 2016-05-19 |
US20180282800A1 (en) | 2018-10-04 |
EP3218519B1 (en) | 2020-12-02 |
CN107002130A (zh) | 2017-08-01 |
DK3218519T3 (da) | 2020-12-21 |
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Address after: 518083 comprehensive building, Beishan Industrial Zone, Yantian District, Guangdong, Shenzhen Patentee after: BGI SHENZHEN Patentee after: Shenzhen Huada Gene Technology Co., Ltd. Address before: 518083 comprehensive building, Beishan Industrial Zone, Yantian District, Shenzhen City, Guangdong Province Patentee before: BGI SHENZHEN Patentee before: Shenzhen Huada Gene Technology Co., Ltd. |
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