CA2852365C - Proteases able to hydrolyze gluten peptides and proteins at acidic ph, from the actinomycete actinoallomurus. - Google Patents
Proteases able to hydrolyze gluten peptides and proteins at acidic ph, from the actinomycete actinoallomurus. Download PDFInfo
- Publication number
- CA2852365C CA2852365C CA2852365A CA2852365A CA2852365C CA 2852365 C CA2852365 C CA 2852365C CA 2852365 A CA2852365 A CA 2852365A CA 2852365 A CA2852365 A CA 2852365A CA 2852365 C CA2852365 C CA 2852365C
- Authority
- CA
- Canada
- Prior art keywords
- endopeptidase
- seq
- endopep
- sequence
- identity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 108010068370 Glutens Proteins 0.000 title claims abstract description 51
- 235000021312 gluten Nutrition 0.000 title claims abstract description 50
- 241000605656 Actinoallomurus Species 0.000 title claims abstract description 32
- 108090000765 processed proteins & peptides Proteins 0.000 title claims description 96
- 108090000623 proteins and genes Proteins 0.000 title claims description 92
- 102000004169 proteins and genes Human genes 0.000 title claims description 80
- 235000018102 proteins Nutrition 0.000 title claims description 77
- 102000035195 Peptidases Human genes 0.000 title claims description 66
- 108091005804 Peptidases Proteins 0.000 title claims description 66
- 102000004196 processed proteins & peptides Human genes 0.000 title claims description 63
- 239000004365 Protease Substances 0.000 title description 45
- 230000002378 acidificating effect Effects 0.000 title description 17
- 241001446247 uncultured actinomycete Species 0.000 title description 4
- 102000005593 Endopeptidases Human genes 0.000 claims abstract description 133
- 108010059378 Endopeptidases Proteins 0.000 claims abstract description 133
- 102000004190 Enzymes Human genes 0.000 claims abstract description 122
- 108090000790 Enzymes Proteins 0.000 claims abstract description 122
- 229940088598 enzyme Drugs 0.000 claims abstract description 122
- 239000000203 mixture Substances 0.000 claims abstract description 82
- 208000015943 Coeliac disease Diseases 0.000 claims abstract description 69
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 43
- 238000000034 method Methods 0.000 claims abstract description 41
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 37
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 37
- 231100000331 toxic Toxicity 0.000 claims abstract description 22
- 230000002588 toxic effect Effects 0.000 claims abstract description 22
- 230000002496 gastric effect Effects 0.000 claims abstract description 19
- 235000013305 food Nutrition 0.000 claims abstract description 18
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 17
- 239000008194 pharmaceutical composition Substances 0.000 claims abstract description 13
- 230000002797 proteolythic effect Effects 0.000 claims abstract description 12
- 208000035475 disorder Diseases 0.000 claims abstract description 11
- 206010012468 Dermatitis herpetiformis Diseases 0.000 claims abstract description 10
- 238000003776 cleavage reaction Methods 0.000 claims abstract description 10
- 230000007017 scission Effects 0.000 claims abstract description 10
- 230000000968 intestinal effect Effects 0.000 claims abstract description 9
- 230000002265 prevention Effects 0.000 claims abstract description 7
- 239000004615 ingredient Substances 0.000 claims abstract description 6
- 210000004027 cell Anatomy 0.000 claims description 60
- 239000012634 fragment Substances 0.000 claims description 47
- 238000004519 manufacturing process Methods 0.000 claims description 23
- 229920001184 polypeptide Polymers 0.000 claims description 20
- 235000015872 dietary supplement Nutrition 0.000 claims description 19
- 239000001963 growth medium Substances 0.000 claims description 19
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 18
- 230000006378 damage Effects 0.000 claims description 15
- 244000005700 microbiome Species 0.000 claims description 13
- 230000035772 mutation Effects 0.000 claims description 13
- 230000008569 process Effects 0.000 claims description 13
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 12
- 108091033319 polynucleotide Proteins 0.000 claims description 12
- 102000040430 polynucleotide Human genes 0.000 claims description 12
- 239000002157 polynucleotide Substances 0.000 claims description 12
- 108010038807 Oligopeptides Proteins 0.000 claims description 11
- 102000015636 Oligopeptides Human genes 0.000 claims description 11
- 241000187747 Streptomyces Species 0.000 claims description 11
- 241000093906 Actinoallomurus sp. Species 0.000 claims description 8
- 241000588724 Escherichia coli Species 0.000 claims description 8
- 239000000047 product Substances 0.000 claims description 7
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 claims description 7
- 241001001250 Catenulispora Species 0.000 claims description 6
- 208000002460 Enteropathy-Associated T-Cell Lymphoma Diseases 0.000 claims description 6
- 208000030836 Hashimoto thyroiditis Diseases 0.000 claims description 6
- 241001430082 Ktedonobacter Species 0.000 claims description 6
- 208000001132 Osteoporosis Diseases 0.000 claims description 6
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 claims description 6
- 208000007502 anemia Diseases 0.000 claims description 6
- 230000002950 deficient Effects 0.000 claims description 6
- 239000003814 drug Substances 0.000 claims description 6
- 229910052742 iron Inorganic materials 0.000 claims description 6
- 241000228212 Aspergillus Species 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 5
- 241000186660 Lactobacillus Species 0.000 claims description 5
- 229940039696 lactobacillus Drugs 0.000 claims description 5
- 239000003531 protein hydrolysate Substances 0.000 claims description 4
- 238000010187 selection method Methods 0.000 claims description 4
- 102100034560 Cytosol aminopeptidase Human genes 0.000 claims description 3
- 241000589516 Pseudomonas Species 0.000 claims description 3
- 241000205160 Pyrococcus Species 0.000 claims description 3
- 230000000593 degrading effect Effects 0.000 claims description 3
- 235000021056 liquid food Nutrition 0.000 claims description 3
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 3
- 241001198387 Escherichia coli BL21(DE3) Species 0.000 claims description 2
- 101710135670 Putative Xaa-Pro dipeptidyl-peptidase Proteins 0.000 claims description 2
- 101710143531 Xaa-Pro dipeptidyl-peptidase Proteins 0.000 claims description 2
- 235000013361 beverage Nutrition 0.000 claims description 2
- 108010017378 prolyl aminopeptidase Proteins 0.000 claims description 2
- 239000008203 oral pharmaceutical composition Substances 0.000 claims 1
- 229940066758 endopeptidases Drugs 0.000 abstract description 35
- 239000000654 additive Substances 0.000 abstract description 2
- 108010061711 Gliadin Proteins 0.000 description 40
- 230000000694 effects Effects 0.000 description 34
- 102000057297 Pepsin A Human genes 0.000 description 28
- 108090000284 Pepsin A Proteins 0.000 description 28
- 229940111202 pepsin Drugs 0.000 description 28
- 238000004458 analytical method Methods 0.000 description 25
- 101710118538 Protease Proteins 0.000 description 20
- 230000015556 catabolic process Effects 0.000 description 20
- 230000029087 digestion Effects 0.000 description 20
- 238000011534 incubation Methods 0.000 description 20
- 235000019419 proteases Nutrition 0.000 description 18
- 239000000758 substrate Substances 0.000 description 18
- 125000003275 alpha amino acid group Chemical group 0.000 description 17
- 238000006731 degradation reaction Methods 0.000 description 17
- 235000019833 protease Nutrition 0.000 description 16
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 15
- 235000001014 amino acid Nutrition 0.000 description 15
- 108090000797 sedolisin Proteins 0.000 description 15
- 150000001413 amino acids Chemical class 0.000 description 12
- 230000014509 gene expression Effects 0.000 description 11
- 238000004949 mass spectrometry Methods 0.000 description 11
- 239000002609 medium Substances 0.000 description 11
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 10
- 230000002255 enzymatic effect Effects 0.000 description 10
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 10
- 238000000746 purification Methods 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 230000004071 biological effect Effects 0.000 description 9
- 230000001965 increasing effect Effects 0.000 description 9
- 238000006467 substitution reaction Methods 0.000 description 9
- 108010043175 succinylalanylalanyl-prolyl-phenylalanine-4-methylcoumaryl-7-amide Proteins 0.000 description 9
- 238000004885 tandem mass spectrometry Methods 0.000 description 9
- 210000001744 T-lymphocyte Anatomy 0.000 description 8
- 238000007792 addition Methods 0.000 description 8
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 8
- 241000186361 Actinobacteria <class> Species 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 7
- 108091028043 Nucleic acid sequence Proteins 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 230000000875 corresponding effect Effects 0.000 description 7
- 239000000499 gel Substances 0.000 description 7
- 239000013612 plasmid Substances 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 6
- 238000013459 approach Methods 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 235000005911 diet Nutrition 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 238000000589 high-performance liquid chromatography-mass spectrometry Methods 0.000 description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 6
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 5
- 240000005979 Hordeum vulgare Species 0.000 description 5
- 235000007340 Hordeum vulgare Nutrition 0.000 description 5
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 5
- 238000012512 characterization method Methods 0.000 description 5
- 239000000306 component Substances 0.000 description 5
- 230000037213 diet Effects 0.000 description 5
- 239000000284 extract Substances 0.000 description 5
- 235000019253 formic acid Nutrition 0.000 description 5
- 210000001035 gastrointestinal tract Anatomy 0.000 description 5
- 230000007062 hydrolysis Effects 0.000 description 5
- 238000006460 hydrolysis reaction Methods 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 244000063299 Bacillus subtilis Species 0.000 description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 description 4
- 102000003779 Dipeptidyl-peptidases and tripeptidyl-peptidases Human genes 0.000 description 4
- 108090000194 Dipeptidyl-peptidases and tripeptidyl-peptidases Proteins 0.000 description 4
- 241000192125 Firmicutes Species 0.000 description 4
- 101000610620 Homo sapiens Putative serine protease 29 Proteins 0.000 description 4
- 101710172072 Kexin Proteins 0.000 description 4
- 206010025476 Malabsorption Diseases 0.000 description 4
- 208000004155 Malabsorption Syndromes Diseases 0.000 description 4
- 101710088675 Proline-rich peptide Proteins 0.000 description 4
- 102100037838 Prolyl endopeptidase Human genes 0.000 description 4
- 108700039882 Protein Glutamine gamma Glutamyltransferase 2 Proteins 0.000 description 4
- 108010029485 Protein Isoforms Proteins 0.000 description 4
- 102000001708 Protein Isoforms Human genes 0.000 description 4
- 108010076504 Protein Sorting Signals Proteins 0.000 description 4
- 102100038095 Protein-glutamine gamma-glutamyltransferase 2 Human genes 0.000 description 4
- 102100040345 Putative serine protease 29 Human genes 0.000 description 4
- 108090000787 Subtilisin Proteins 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 239000006071 cream Substances 0.000 description 4
- 238000012217 deletion Methods 0.000 description 4
- 230000037430 deletion Effects 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 230000037406 food intake Effects 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 238000003306 harvesting Methods 0.000 description 4
- 238000001819 mass spectrum Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 235000012054 meals Nutrition 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 210000004400 mucous membrane Anatomy 0.000 description 4
- 239000002773 nucleotide Substances 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000014616 translation Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- HZWWPUTXBJEENE-UHFFFAOYSA-N 5-amino-2-[[1-[5-amino-2-[[1-[2-amino-3-(4-hydroxyphenyl)propanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoic acid Chemical compound C1CCC(C(=O)NC(CCC(N)=O)C(=O)N2C(CCC2)C(=O)NC(CCC(N)=O)C(O)=O)N1C(=O)C(N)CC1=CC=C(O)C=C1 HZWWPUTXBJEENE-UHFFFAOYSA-N 0.000 description 3
- 208000023275 Autoimmune disease Diseases 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108020004511 Recombinant DNA Proteins 0.000 description 3
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 241000209140 Triticum Species 0.000 description 3
- 235000021307 Triticum Nutrition 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 239000008351 acetate buffer Substances 0.000 description 3
- 125000000539 amino acid group Chemical group 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 230000001079 digestive effect Effects 0.000 description 3
- 230000008034 disappearance Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 231100000110 immunotoxic Toxicity 0.000 description 3
- 230000002625 immunotoxic effect Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000005040 ion trap Methods 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 230000035790 physiological processes and functions Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 229910052709 silver Inorganic materials 0.000 description 3
- 239000004332 silver Substances 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 238000002560 therapeutic procedure Methods 0.000 description 3
- 230000009261 transgenic effect Effects 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 208000002109 Argyria Diseases 0.000 description 2
- 241000308822 Aspergillus fumigatus Af293 Species 0.000 description 2
- 241000228245 Aspergillus niger Species 0.000 description 2
- 240000006439 Aspergillus oryzae Species 0.000 description 2
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000919932 Catenulispora acidiphila Species 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 102000029816 Collagenase Human genes 0.000 description 2
- 108060005980 Collagenase Proteins 0.000 description 2
- 102000016622 Dipeptidyl Peptidase 4 Human genes 0.000 description 2
- 101000930822 Giardia intestinalis Dipeptidyl-peptidase 4 Proteins 0.000 description 2
- 102100025255 Haptoglobin Human genes 0.000 description 2
- 101001086426 Homo sapiens Olfactory receptor 1J2 Proteins 0.000 description 2
- 108010004098 Leucyl aminopeptidase Proteins 0.000 description 2
- 102000002704 Leucyl aminopeptidase Human genes 0.000 description 2
- 102000004882 Lipase Human genes 0.000 description 2
- 108090001060 Lipase Proteins 0.000 description 2
- 239000004367 Lipase Substances 0.000 description 2
- 241000579835 Merops Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 208000031888 Mycoses Diseases 0.000 description 2
- 241000736107 Novosphingobium capsulatum Species 0.000 description 2
- 102100032722 Olfactory receptor 1J2 Human genes 0.000 description 2
- 102000056251 Prolyl Oligopeptidases Human genes 0.000 description 2
- 241001257175 Rugosimonospora Species 0.000 description 2
- 108091006629 SLC13A2 Proteins 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 241000209056 Secale Species 0.000 description 2
- 235000007238 Secale cereale Nutrition 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000319051 Streptacidiphilus Species 0.000 description 2
- 102000004142 Trypsin Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 150000001793 charged compounds Chemical class 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 229960002424 collagenase Drugs 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 210000001842 enterocyte Anatomy 0.000 description 2
- 230000006862 enzymatic digestion Effects 0.000 description 2
- 230000007717 exclusion Effects 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 108020001507 fusion proteins Proteins 0.000 description 2
- 102000037865 fusion proteins Human genes 0.000 description 2
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 2
- 125000000404 glutamine group Chemical group N[C@@H](CCC(N)=O)C(=O)* 0.000 description 2
- 108091005608 glycosylated proteins Proteins 0.000 description 2
- 102000035122 glycosylated proteins Human genes 0.000 description 2
- 238000012405 in silico analysis Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000015788 innate immune response Effects 0.000 description 2
- 210000004347 intestinal mucosa Anatomy 0.000 description 2
- 238000004255 ion exchange chromatography Methods 0.000 description 2
- 230000005865 ionizing radiation Effects 0.000 description 2
- 235000019421 lipase Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 210000000110 microvilli Anatomy 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 239000003471 mutagenic agent Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000004007 reversed phase HPLC Methods 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- 229960001322 trypsin Drugs 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- 108010027843 zonulin Proteins 0.000 description 2
- 238000007805 zymography Methods 0.000 description 2
- IOIKVMQQNXHELZ-UHFFFAOYSA-N 4-amino-3-methylchromen-2-one Chemical compound C1=CC=C2OC(=O)C(C)=C(N)C2=C1 IOIKVMQQNXHELZ-UHFFFAOYSA-N 0.000 description 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 108010051457 Acid Phosphatase Proteins 0.000 description 1
- 102000013563 Acid Phosphatase Human genes 0.000 description 1
- 241000203809 Actinomycetales Species 0.000 description 1
- 241001001804 Actinospica Species 0.000 description 1
- 241000919936 Actinospica acidiphila Species 0.000 description 1
- 241000921014 Actinospica robiniae Species 0.000 description 1
- 241001001795 Actinospicaceae Species 0.000 description 1
- 241001147780 Alicyclobacillus Species 0.000 description 1
- 108090000915 Aminopeptidases Proteins 0.000 description 1
- 102000004400 Aminopeptidases Human genes 0.000 description 1
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- 239000005695 Ammonium acetate Substances 0.000 description 1
- 241001225321 Aspergillus fumigatus Species 0.000 description 1
- 240000001009 Aspergillus oryzae RIB40 Species 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 1
- 108091005658 Basic proteases Proteins 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- 101710134031 CCAAT/enhancer-binding protein beta Proteins 0.000 description 1
- 102100035793 CD83 antigen Human genes 0.000 description 1
- 241001001255 Catenulisporaceae Species 0.000 description 1
- 241001001796 Catenulisporales Species 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 208000011231 Crohn disease Diseases 0.000 description 1
- 108010037462 Cyclooxygenase 2 Proteins 0.000 description 1
- 108010005843 Cysteine Proteases Proteins 0.000 description 1
- 102000005927 Cysteine Proteases Human genes 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 101710199286 Cytosol aminopeptidase Proteins 0.000 description 1
- 102100028717 Cytosolic 5'-nucleotidase 3A Human genes 0.000 description 1
- 201000004624 Dermatitis Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 101710197780 E3 ubiquitin-protein ligase LAP Proteins 0.000 description 1
- 241000589566 Elizabethkingia meningoseptica Species 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 102000018389 Exopeptidases Human genes 0.000 description 1
- 108010091443 Exopeptidases Proteins 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 206010064571 Gene mutation Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 description 1
- 102000005744 Glycoside Hydrolases Human genes 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 108091006054 His-tagged proteins Proteins 0.000 description 1
- 101000946856 Homo sapiens CD83 antigen Proteins 0.000 description 1
- 101001057504 Homo sapiens Interferon-stimulated gene 20 kDa protein Proteins 0.000 description 1
- 101001055144 Homo sapiens Interleukin-2 receptor subunit alpha Proteins 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 102000008070 Interferon-gamma Human genes 0.000 description 1
- 108010074328 Interferon-gamma Proteins 0.000 description 1
- 102100027268 Interferon-stimulated gene 20 kDa protein Human genes 0.000 description 1
- 241001138401 Kluyveromyces lactis Species 0.000 description 1
- 241001430080 Ktedonobacter racemifer Species 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical group CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical group OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 108010059881 Lactase Proteins 0.000 description 1
- 244000199866 Lactobacillus casei Species 0.000 description 1
- 235000013958 Lactobacillus casei Nutrition 0.000 description 1
- 241000194036 Lactococcus Species 0.000 description 1
- 201000010538 Lactose Intolerance Diseases 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Chemical group CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 241000192132 Leuconostoc Species 0.000 description 1
- 241000219745 Lupinus Species 0.000 description 1
- 239000006137 Luria-Bertani broth Substances 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 101710204480 Lysosomal acid phosphatase Proteins 0.000 description 1
- 101710132682 Lysozyme 1 Proteins 0.000 description 1
- 238000007476 Maximum Likelihood Methods 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 108090000301 Membrane transport proteins Proteins 0.000 description 1
- 102000003939 Membrane transport proteins Human genes 0.000 description 1
- 241000187708 Micromonospora Species 0.000 description 1
- 241001134635 Micromonosporaceae Species 0.000 description 1
- 239000012901 Milli-Q water Substances 0.000 description 1
- 241000863422 Myxococcus xanthus Species 0.000 description 1
- 101100477212 Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) sed4 gene Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000192001 Pediococcus Species 0.000 description 1
- 102000007079 Peptide Fragments Human genes 0.000 description 1
- 108010033276 Peptide Fragments Proteins 0.000 description 1
- 102100030887 Pleckstrin homology-like domain family A member 1 Human genes 0.000 description 1
- 101710134452 Pleckstrin homology-like domain family A member 1 Proteins 0.000 description 1
- 102000029797 Prion Human genes 0.000 description 1
- 108091000054 Prion Proteins 0.000 description 1
- 101710089118 Probable cytosol aminopeptidase Proteins 0.000 description 1
- 101710083337 Probable tripeptidyl-peptidase SED2 Proteins 0.000 description 1
- 108700015930 Prolyl Oligopeptidases Proteins 0.000 description 1
- 101710178372 Prolyl endopeptidase Proteins 0.000 description 1
- 102100038280 Prostaglandin G/H synthase 2 Human genes 0.000 description 1
- 108010026552 Proteome Proteins 0.000 description 1
- 244000097202 Rathbunia alamosensis Species 0.000 description 1
- 235000009776 Rathbunia alamosensis Nutrition 0.000 description 1
- 241000521390 Rugosimonospora acidiphila Species 0.000 description 1
- 241000521392 Rugosimonospora africana Species 0.000 description 1
- 238000010847 SEQUEST Methods 0.000 description 1
- 101100397225 Schizosaccharomyces pombe (strain 972 / ATCC 24843) isp3 gene Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241001446311 Streptomyces coelicolor A3(2) Species 0.000 description 1
- 241000187398 Streptomyces lividans Species 0.000 description 1
- 241001312734 Streptomyces parvulus Species 0.000 description 1
- 241000204060 Streptomycetaceae Species 0.000 description 1
- 108010033576 Transferrin Receptors Proteins 0.000 description 1
- 102000007238 Transferrin Receptors Human genes 0.000 description 1
- 241000223238 Trichophyton Species 0.000 description 1
- 101710100305 Tripeptidyl-peptidase SED2 Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241001148118 Xanthomonas sp. Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000004721 adaptive immunity Effects 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000005377 adsorption chromatography Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 208000030961 allergic reaction Diseases 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- 229940043376 ammonium acetate Drugs 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 239000001166 ammonium sulphate Substances 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 230000030741 antigen processing and presentation Effects 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 229940091771 aspergillus fumigatus Drugs 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 210000004082 barrier epithelial cell Anatomy 0.000 description 1
- 238000002869 basic local alignment search tool Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 238000007622 bioinformatic analysis Methods 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000004464 cereal grain Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229920001429 chelating resin Polymers 0.000 description 1
- UHZZMRAGKVHANO-UHFFFAOYSA-M chlormequat chloride Chemical compound [Cl-].C[N+](C)(C)CCCl UHZZMRAGKVHANO-UHFFFAOYSA-M 0.000 description 1
- 239000003593 chromogenic compound Substances 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 230000000254 damaging effect Effects 0.000 description 1
- 230000006240 deamidation Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 229940108890 emend Drugs 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 230000003241 endoproteolytic effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000004890 epithelial barrier function Effects 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000005428 food component Substances 0.000 description 1
- 210000003918 fraction a Anatomy 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-L glutamate group Chemical group N[C@@H](CCC(=O)[O-])C(=O)[O-] WHUUTDBJXJRKMK-VKHMYHEASA-L 0.000 description 1
- 235000006171 gluten free diet Nutrition 0.000 description 1
- 235000020884 gluten-free diet Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 230000008073 immune recognition Effects 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 230000002998 immunogenetic effect Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 229960003130 interferon gamma Drugs 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 229940116108 lactase Drugs 0.000 description 1
- 229940017800 lactobacillus casei Drugs 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 230000016379 mucosal immune response Effects 0.000 description 1
- 230000004677 mucosal permeability Effects 0.000 description 1
- 235000013557 nattō Nutrition 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000031787 nutrient reservoir activity Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 231100000255 pathogenic effect Toxicity 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 238000003359 percent control normalization Methods 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Chemical group OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N phenylalanine group Chemical group N[C@@H](CC1=CC=CC=C1)C(=O)O COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000007112 pro inflammatory response Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 238000012514 protein characterization Methods 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000000575 proteomic method Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- QEVHRUUCFGRFIF-MDEJGZGSSA-N reserpine Chemical compound O([C@H]1[C@@H]([C@H]([C@H]2C[C@@H]3C4=C(C5=CC=C(OC)C=C5N4)CCN3C[C@H]2C1)C(=O)OC)OC)C(=O)C1=CC(OC)=C(OC)C(OC)=C1 QEVHRUUCFGRFIF-MDEJGZGSSA-N 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000012465 retentate Substances 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 238000010563 solid-state fermentation Methods 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000013179 statistical model Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000031998 transcytosis Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 238000000539 two dimensional gel electrophoresis Methods 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
- A61K38/482—Serine endopeptidases (3.4.21)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21026—Prolyl oligopeptidase (3.4.21.26), i.e. proline-specific endopeptidase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Zoology (AREA)
- Epidemiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Wood Science & Technology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Dermatology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11425291 | 2011-12-06 | ||
| EP11425291.9 | 2011-12-06 | ||
| PCT/EP2012/071816 WO2013083338A1 (en) | 2011-12-06 | 2012-11-05 | New proteases able to hydrolyze gluten peptides and proteins at acidic ph, from the actinomycete actinoallomurus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2852365A1 CA2852365A1 (en) | 2013-06-13 |
| CA2852365C true CA2852365C (en) | 2021-01-26 |
Family
ID=47137719
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2852365A Active CA2852365C (en) | 2011-12-06 | 2012-11-05 | Proteases able to hydrolyze gluten peptides and proteins at acidic ph, from the actinomycete actinoallomurus. |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US20140356345A1 (enExample) |
| EP (1) | EP2788018B1 (enExample) |
| JP (1) | JP6203743B2 (enExample) |
| CN (1) | CN104039345B (enExample) |
| AU (1) | AU2012348782B2 (enExample) |
| BR (1) | BR112014012100B1 (enExample) |
| CA (1) | CA2852365C (enExample) |
| EA (1) | EA028228B1 (enExample) |
| ES (1) | ES2595500T3 (enExample) |
| IN (1) | IN2014CN04131A (enExample) |
| WO (1) | WO2013083338A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2718434B1 (en) | 2011-08-10 | 2018-03-28 | University of Washington through its Center for Commercialization | Compositions and methods for treating celiac sprue disease |
| EP3520808B1 (en) * | 2013-08-14 | 2021-04-21 | University Of Washington Through Its Center For Commercialization | Compositions for treating celiac sprue disease |
| WO2015032620A1 (en) | 2013-09-05 | 2015-03-12 | Dsm Ip Assets B.V. | Use of an acidic soft drink to enhance the efficacy of a gluten-digesting enzyme |
| US10308967B2 (en) * | 2013-11-26 | 2019-06-04 | Novozymes A/S | Milling process |
| DK3209774T3 (da) * | 2014-10-24 | 2020-05-25 | Dupont Nutrition Biosci Aps | Anvendelse af prolintolerante tripeptidylpeptidaser i foderadditivsammensætninger |
| MX392140B (es) | 2015-06-08 | 2025-03-21 | Univ Washington | Composiciones y uso de las mismas para tratar la enfermedad celiaca. |
| US11124785B2 (en) | 2016-02-11 | 2021-09-21 | Trustees Of Boston University | Rothia subtilisins, S8A family proteases, as therapeutic enzymes for application in gluten intolerance disorders |
| IT201900012942A1 (it) | 2019-07-25 | 2021-01-25 | Nemysis Ltd | Metodo per la produzione di una composizione enzimatica comprendente una endopeptidasi ricombinante |
| WO2021013553A1 (en) | 2019-07-25 | 2021-01-28 | Nemysis Limited | Method for the production of an enzymatic composition comprising a recombinant endopeptidase |
| CN111172138B (zh) * | 2020-02-14 | 2022-03-25 | 江南大学 | 一种蛋白水解酶及其在蛋白肽生产中的应用 |
| IT202100018254A1 (it) | 2021-07-12 | 2023-01-12 | Nemysis Ltd | New systems for producing recombinant proteins/ nuovi sistemi di produzione di proteine ricombinanti |
| CN114438044B (zh) * | 2022-01-14 | 2024-03-29 | 南昌大学 | 一种高效降解麸质蛋白的多酶复合体及其应用 |
| WO2024233959A1 (en) * | 2023-05-11 | 2024-11-14 | Digestiva, Inc. | Wine dehazing methods and compositions |
| WO2025067635A1 (en) * | 2023-09-26 | 2025-04-03 | Amyra Biotech Ag | Pharmaceutical compositions comprising exopeptidases and uses thereof |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3104935B2 (ja) | 1992-05-28 | 2000-10-30 | 株式会社ブリヂストン | 空気入りタイヤ |
| EP2364718B1 (en) | 2002-02-14 | 2016-07-06 | The Board of Trustees of the Leland Stanford Junior University | Enzyme treatment of foodstuffs for celiac sprue |
| US7320788B2 (en) * | 2002-02-14 | 2008-01-22 | The Board Of Trustees Of The Leland Stanford Junior University | Enzyme treatment of foodstuffs for Celiac Sprue |
| US7265093B2 (en) * | 2002-05-14 | 2007-09-04 | The Board Of Trustees Of The Leland Stanford Junior University | Drug therapy for Celiac Sprue |
| CN1871351B (zh) | 2003-08-25 | 2010-06-23 | 富诺齐梅生物技术股份有限公司 | 一种新的真菌蛋白及其编码核酸 |
| DK1663298T3 (da) | 2003-09-23 | 2019-10-14 | Dsm Ip Assets Bv | Anvendelse af prolinspecifikke endoproteaser til hydrolyse af peptider og proteiner |
| NL1027650C1 (nl) | 2004-12-03 | 2006-06-07 | Leonardus Antonius Luttikhold | Transporthulpmiddel voor deuren en panelen. |
| EP1985698A1 (en) * | 2007-04-25 | 2008-10-29 | Nestec S.A. | Peptidases from basidiomycetes |
| US20120276075A1 (en) | 2009-12-21 | 2012-11-01 | Centre Hospitalier Universitaire Vaudois (Chuv) | Synergic action of a prolyl protease and tripeptidyl proteases |
| EP2531521B1 (en) * | 2010-02-02 | 2017-09-06 | Amano Enzyme USA., Ltd. | Use of proteases for gluten intolerance |
| EP2555792A4 (en) * | 2010-03-30 | 2013-11-06 | Alvine Pharmaceuticals Inc | PROTEASES DEGRADING GLUTEN |
| JP2011211997A (ja) * | 2010-04-02 | 2011-10-27 | Astellas Pharma Inc | アントラサイクリン化合物を生産する微生物 |
-
2012
- 2012-11-05 CA CA2852365A patent/CA2852365C/en active Active
- 2012-11-05 BR BR112014012100-1A patent/BR112014012100B1/pt active IP Right Grant
- 2012-11-05 WO PCT/EP2012/071816 patent/WO2013083338A1/en not_active Ceased
- 2012-11-05 AU AU2012348782A patent/AU2012348782B2/en active Active
- 2012-11-05 ES ES12781098.4T patent/ES2595500T3/es active Active
- 2012-11-05 IN IN4131CHN2014 patent/IN2014CN04131A/en unknown
- 2012-11-05 EP EP12781098.4A patent/EP2788018B1/en active Active
- 2012-11-05 CN CN201280060514.5A patent/CN104039345B/zh active Active
- 2012-11-05 US US14/356,122 patent/US20140356345A1/en not_active Abandoned
- 2012-11-05 EA EA201491117A patent/EA028228B1/ru not_active IP Right Cessation
- 2012-11-05 JP JP2014545146A patent/JP6203743B2/ja active Active
-
2015
- 2015-11-23 US US14/949,503 patent/US10201593B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| AU2012348782B2 (en) | 2017-10-26 |
| JP6203743B2 (ja) | 2017-09-27 |
| IN2014CN04131A (enExample) | 2015-07-17 |
| WO2013083338A1 (en) | 2013-06-13 |
| US20140356345A1 (en) | 2014-12-04 |
| EA028228B1 (ru) | 2017-10-31 |
| BR112014012100B1 (pt) | 2023-04-18 |
| US10201593B2 (en) | 2019-02-12 |
| BR112014012100A2 (pt) | 2020-10-27 |
| CA2852365A1 (en) | 2013-06-13 |
| ES2595500T3 (es) | 2016-12-30 |
| JP2015500641A (ja) | 2015-01-08 |
| AU2012348782A1 (en) | 2014-05-15 |
| CN104039345A (zh) | 2014-09-10 |
| HK1198244A1 (en) | 2015-03-20 |
| US20160114009A1 (en) | 2016-04-28 |
| EP2788018A1 (en) | 2014-10-15 |
| EP2788018B1 (en) | 2016-06-29 |
| EA201491117A1 (ru) | 2014-09-30 |
| CN104039345B (zh) | 2016-11-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2852365C (en) | Proteases able to hydrolyze gluten peptides and proteins at acidic ph, from the actinomycete actinoallomurus. | |
| AU2010334383B2 (en) | Synergic action of a prolyl protease and tripeptidyl proteases | |
| US20140206036A1 (en) | Use of proline specific endoproteases to hydrolyse peptides and proteins | |
| US20240158774A1 (en) | Compositions and Methods for Treating Celiac Sprue Disease | |
| Alvarez-Sieiro et al. | Generation of food-grade recombinant Lactobacillus casei delivering Myxococcus xanthus prolyl endopeptidase | |
| US20220364069A1 (en) | Method for the production of an enzymatic composition comprising a recombinant endopeptidase | |
| HK1198244B (en) | New proteases able to hydrolyze gluten peptides and proteins at acidic ph, from the actinomycete actinoallomurus | |
| US20150197738A1 (en) | Acid Stable Prolyl Endopeptidases for Degrading Gluten | |
| WO2016120764A1 (en) | Aspergillus oryzae prolyl endopeptidases and use thereof in degradation of polypeptides | |
| IT201900012942A1 (it) | Metodo per la produzione di una composizione enzimatica comprendente una endopeptidasi ricombinante |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20151125 |