EA201491117A1 - НОВЫЕ ПРОТЕАЗЫ ИЗ АКТИНОМИЦЕТА ACTINOALLOMURUS, СПОСОБНЫЕ ГИДРОЛИЗОВАТЬ ГЛЮТЕНОВЫЕ ПЕПТИДЫ И БЕЛКИ ПРИ КИСЛОТНОМ pH - Google Patents

НОВЫЕ ПРОТЕАЗЫ ИЗ АКТИНОМИЦЕТА ACTINOALLOMURUS, СПОСОБНЫЕ ГИДРОЛИЗОВАТЬ ГЛЮТЕНОВЫЕ ПЕПТИДЫ И БЕЛКИ ПРИ КИСЛОТНОМ pH

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Publication number
EA201491117A1
EA201491117A1 EA201491117A EA201491117A EA201491117A1 EA 201491117 A1 EA201491117 A1 EA 201491117A1 EA 201491117 A EA201491117 A EA 201491117A EA 201491117 A EA201491117 A EA 201491117A EA 201491117 A1 EA201491117 A1 EA 201491117A1
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EA
Eurasian Patent Office
Prior art keywords
actinoallomurus
endopeptidases
gluten
enzymes
nucleic acids
Prior art date
Application number
EA201491117A
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English (en)
Other versions
EA028228B1 (ru
Inventor
Линда Кавалетти
Лючия Каррано
Моника Аббонди
Мара Брунати
Анна Таравелла
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Фондацьоне Иституто Инсубрико Ди Ричерка Пер Ла Вита
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Application filed by Фондацьоне Иституто Инсубрико Ди Ричерка Пер Ла Вита filed Critical Фондацьоне Иституто Инсубрико Ди Ричерка Пер Ла Вита
Publication of EA201491117A1 publication Critical patent/EA201491117A1/ru
Publication of EA028228B1 publication Critical patent/EA028228B1/ru

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/482—Serine endopeptidases (3.4.21)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
    • A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21026—Prolyl oligopeptidase (3.4.21.26), i.e. proline-specific endopeptidase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Organic Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Dermatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

Изобретение относится к новому семейству протеолитических ферментов, обладающих способностью гидролизовать при рН между 3 и 8 глютеновые олигопептиды, которые являются устойчивыми к расщеплению посредством желудочных и панкреатических ферментов, и чье присутствие в просвете кишечника приводит в результате к токсическим эффектам. Ферменты были идентифицированы как эндопептидазы из семейства S8/S53 и продуцируются штаммом Actinoallomurus. Цель изобретения включает также способы получения ферментной композиции, содержащей эндопептидазы, посредством культивирования нативных штаммов Actinoallomurus, их мутантов или рекомбинантных клеток-хозяев, содержащих нуклеиновые кислоты, кодирующие эндопептидазы. Указанные нуклеиновые кислоты составляют дополнительную цель изобретения. Ферментные композиции, содержащие по меньшей мере одну эндопептидазу изобретения, являются применимыми для лечения и/или предотвращения глютенчувствительной целиакии, герпетиформного дерматита и любого другого расстройства, ассоциированного с непереносимостью клейковины в качестве ингредиентов фармацевтических композиций или в качестве добавок в пищевые продукты и напитки.
EA201491117A 2011-12-06 2012-11-05 Гидролизующая глютеновые олигопептиды эндопептидазная композиция, способ ее получения и применение EA028228B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11425291 2011-12-06
PCT/EP2012/071816 WO2013083338A1 (en) 2011-12-06 2012-11-05 New proteases able to hydrolyze gluten peptides and proteins at acidic ph, from the actinomycete actinoallomurus

Publications (2)

Publication Number Publication Date
EA201491117A1 true EA201491117A1 (ru) 2014-09-30
EA028228B1 EA028228B1 (ru) 2017-10-31

Family

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EA201491117A EA028228B1 (ru) 2011-12-06 2012-11-05 Гидролизующая глютеновые олигопептиды эндопептидазная композиция, способ ее получения и применение

Country Status (12)

Country Link
US (2) US20140356345A1 (ru)
EP (1) EP2788018B1 (ru)
JP (1) JP6203743B2 (ru)
CN (1) CN104039345B (ru)
AU (1) AU2012348782B2 (ru)
BR (1) BR112014012100B1 (ru)
CA (1) CA2852365C (ru)
EA (1) EA028228B1 (ru)
ES (1) ES2595500T3 (ru)
HK (1) HK1198244A1 (ru)
IN (1) IN2014CN04131A (ru)
WO (1) WO2013083338A1 (ru)

Cited By (1)

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CN111172138A (zh) * 2020-02-14 2020-05-19 江南大学 一种蛋白水解酶及其在蛋白肽生产中的应用

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EP4023243A3 (en) 2011-08-10 2022-09-14 University of Washington through its Center for Commercialization Compositions and methods for treating celiac sprue disease
WO2015023728A1 (en) * 2013-08-14 2015-02-19 University Of Washington Through Its Center For Commercialization Compositions and methods for treating celiac sprue disease
US9867872B2 (en) 2013-09-05 2018-01-16 Dsm Ip Assets B.V. Use of an acidic soft drink to enhance the efficacy of a gluten-digesting enzyme
WO2015078372A1 (en) * 2013-11-26 2015-06-04 Novozymes A/S Milling process
WO2016062855A1 (en) * 2014-10-24 2016-04-28 Dupont Nutrition Biosciences Aps Proline tolerant tripeptidyl peptidases and uses thereof
CR20170567A (es) 2015-06-08 2018-06-14 Univ California Composiciones y métodos para tratar la enfermedad celiaca.
US11124785B2 (en) 2016-02-11 2021-09-21 Trustees Of Boston University Rothia subtilisins, S8A family proteases, as therapeutic enzymes for application in gluten intolerance disorders
IT201900012942A1 (it) 2019-07-25 2021-01-25 Nemysis Ltd Metodo per la produzione di una composizione enzimatica comprendente una endopeptidasi ricombinante
BR112022000954A2 (pt) 2019-07-25 2022-05-24 Nemysis Ltd Método para a produção de uma composição enzimática compreendendo uma endopeptidase recombinante
IT202100018254A1 (it) 2021-07-12 2023-01-12 Nemysis Ltd New systems for producing recombinant proteins/ nuovi sistemi di produzione di proteine ricombinanti
CN114438044B (zh) * 2022-01-14 2024-03-29 南昌大学 一种高效降解麸质蛋白的多酶复合体及其应用

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JP3104935B2 (ja) 1992-05-28 2000-10-30 株式会社ブリヂストン 空気入りタイヤ
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US7320788B2 (en) * 2002-02-14 2008-01-22 The Board Of Trustees Of The Leland Stanford Junior University Enzyme treatment of foodstuffs for Celiac Sprue
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111172138A (zh) * 2020-02-14 2020-05-19 江南大学 一种蛋白水解酶及其在蛋白肽生产中的应用
CN111172138B (zh) * 2020-02-14 2022-03-25 江南大学 一种蛋白水解酶及其在蛋白肽生产中的应用

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Publication number Publication date
CN104039345B (zh) 2016-11-09
US10201593B2 (en) 2019-02-12
CN104039345A (zh) 2014-09-10
BR112014012100A2 (pt) 2020-10-27
WO2013083338A1 (en) 2013-06-13
JP2015500641A (ja) 2015-01-08
AU2012348782A1 (en) 2014-05-15
BR112014012100B1 (pt) 2023-04-18
AU2012348782B2 (en) 2017-10-26
EP2788018A1 (en) 2014-10-15
US20140356345A1 (en) 2014-12-04
EA028228B1 (ru) 2017-10-31
ES2595500T3 (es) 2016-12-30
CA2852365A1 (en) 2013-06-13
HK1198244A1 (en) 2015-03-20
IN2014CN04131A (ru) 2015-07-17
JP6203743B2 (ja) 2017-09-27
CA2852365C (en) 2021-01-26
US20160114009A1 (en) 2016-04-28
EP2788018B1 (en) 2016-06-29

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