CA2766351C - Chimeric primers with hairpin conformations and methods of using same - Google Patents
Chimeric primers with hairpin conformations and methods of using same Download PDFInfo
- Publication number
- CA2766351C CA2766351C CA2766351A CA2766351A CA2766351C CA 2766351 C CA2766351 C CA 2766351C CA 2766351 A CA2766351 A CA 2766351A CA 2766351 A CA2766351 A CA 2766351A CA 2766351 C CA2766351 C CA 2766351C
- Authority
- CA
- Canada
- Prior art keywords
- sequence
- tag
- nucleic acid
- tag sequence
- primer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US22127109P | 2009-06-29 | 2009-06-29 | |
| US61/221,271 | 2009-06-29 | ||
| PCT/US2010/040354 WO2011008530A2 (en) | 2009-06-29 | 2010-06-29 | Chimeric primers with hairpin conformations and methods of using same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2766351A1 CA2766351A1 (en) | 2011-01-20 |
| CA2766351C true CA2766351C (en) | 2018-02-27 |
Family
ID=43381153
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2766351A Active CA2766351C (en) | 2009-06-29 | 2010-06-29 | Chimeric primers with hairpin conformations and methods of using same |
Country Status (6)
| Country | Link |
|---|---|
| US (6) | US9587270B2 (https=) |
| EP (2) | EP2449104B1 (https=) |
| JP (3) | JP5785942B2 (https=) |
| CN (1) | CN102686728B (https=) |
| CA (1) | CA2766351C (https=) |
| WO (1) | WO2011008530A2 (https=) |
Families Citing this family (72)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102007029772B4 (de) * | 2007-06-22 | 2011-12-08 | Aj Innuscreen Gmbh | Verfahren und Schnelltest zum Nachweis spezifischer Nukleinsäuresequenzen |
| US9175339B2 (en) * | 2009-10-29 | 2015-11-03 | Ngk Insulators, Ltd. | Method for detection of target nucleic acid |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9850527B2 (en) * | 2010-06-14 | 2017-12-26 | National University Of Singapore | Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative PCR |
| SG190377A1 (en) | 2010-11-24 | 2013-06-28 | Kaneka Corp | Amplified nucleic acid detection method and detection device |
| JP5503021B2 (ja) * | 2011-09-14 | 2014-05-28 | 日本碍子株式会社 | 標的核酸の検出方法 |
| KR20140066782A (ko) | 2011-09-29 | 2014-06-02 | 루미넥스 코포레이션 | 가수분해 프로브 |
| US10385392B2 (en) | 2011-12-30 | 2019-08-20 | Abbott Molecular Inc. | Nucleic acid hybridization probes |
| CA2865575C (en) | 2012-02-27 | 2024-01-16 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| JP6219816B2 (ja) | 2012-04-27 | 2017-10-25 | 株式会社カネカ | 核酸の増幅方法、および、増幅核酸の検出方法 |
| CN104838014B (zh) * | 2012-10-15 | 2017-06-30 | 生命技术公司 | 用于标靶核酸富集的组合物、方法、系统和试剂盒 |
| CA2901907C (en) * | 2013-02-20 | 2023-06-20 | Emory University | Methods of sequencing nucleic acids in mixtures and compositions related thereto |
| CN105555971B (zh) * | 2013-08-09 | 2017-08-29 | 卢米耐克斯公司 | 用于在核酸测定中改善熔链分辨和多重性的探针 |
| KR102758333B1 (ko) | 2013-08-28 | 2025-01-23 | 벡톤 디킨슨 앤드 컴퍼니 | 대량의 동시 단일 세포 분석 |
| US9582877B2 (en) | 2013-10-07 | 2017-02-28 | Cellular Research, Inc. | Methods and systems for digitally counting features on arrays |
| WO2015076356A1 (ja) | 2013-11-22 | 2015-05-28 | 株式会社カネカ | 短鎖ｒｎａの検出方法 |
| US10975371B2 (en) * | 2014-04-29 | 2021-04-13 | Illumina, Inc. | Nucleic acid sequence analysis from single cells |
| WO2015193483A1 (en) * | 2014-06-19 | 2015-12-23 | Multiplicom N.V. | Restriction mediated quantitative polymerase chain reactions |
| US10011861B2 (en) | 2014-08-14 | 2018-07-03 | Luminex Corporation | Cleavable hairpin primers |
| EP3259371B1 (en) | 2015-02-19 | 2020-09-02 | Becton, Dickinson and Company | High-throughput single-cell analysis combining proteomic and genomic information |
| US10557134B2 (en) * | 2015-02-24 | 2020-02-11 | Trustees Of Boston University | Protection of barcodes during DNA amplification using molecular hairpins |
| WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| EP4582556A3 (en) | 2015-04-23 | 2025-10-08 | Becton, Dickinson and Company | Methods and compositions for whole transcriptome amplification |
| WO2016196229A1 (en) | 2015-06-01 | 2016-12-08 | Cellular Research, Inc. | Methods for rna quantification |
| JP6940484B2 (ja) | 2015-09-11 | 2021-09-29 | セルラー リサーチ， インコーポレイテッド | ライブラリー正規化のための方法および組成物 |
| JP7129343B2 (ja) | 2016-05-02 | 2022-09-01 | ベクトン・ディキンソン・アンド・カンパニー | 正確な分子バーコーディング |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| ES2979395T3 (es) | 2016-05-26 | 2024-09-25 | Becton Dickinson Co | Métodos de ajuste del recuento de etiquetas moleculares |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| CN109791157B (zh) | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| US11164659B2 (en) | 2016-11-08 | 2021-11-02 | Becton, Dickinson And Company | Methods for expression profile classification |
| JP7228510B2 (ja) | 2016-11-08 | 2023-02-24 | ベクトン・ディキンソン・アンド・カンパニー | 細胞標識分類の方法 |
| ES2961580T3 (es) | 2017-01-13 | 2024-03-12 | Cellular Res Inc | Revestimiento hidrófilo de canales de fluidos |
| CN110382708A (zh) | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | 使用阻断性寡核苷酸进行选择性扩增 |
| EP3601593B1 (en) * | 2017-03-24 | 2021-12-22 | Bio-Rad Laboratories, Inc. | Universal hairpin primers |
| CN108546748A (zh) * | 2017-04-05 | 2018-09-18 | 杭州丹威生物科技有限公司 | 一种检测核酸的方法和试剂盒 |
| CN108690875A (zh) * | 2017-04-05 | 2018-10-23 | 杭州丹威生物科技有限公司 | 用来筛查传染病基因和遗传改变的带有条码的微阵列芯片及使用方法 |
| CN110719959B (zh) | 2017-06-05 | 2021-08-06 | 贝克顿迪金森公司 | 针对单细胞的样品索引 |
| KR101899371B1 (ko) | 2017-07-25 | 2018-10-29 | (주)엔바이오텍 | 핵산 복합체 페어, 핵산 복합체 페어를 포함하는 pcr용 키트, 및 핵산 복합체 페어를 이용한 타겟 검출 방법 |
| EP3728636B1 (en) | 2017-12-19 | 2024-09-11 | Becton, Dickinson and Company | Particles associated with oligonucleotides |
| EA202091646A1 (ru) * | 2018-01-05 | 2020-11-17 | Квошент Сюисс Са | Платформа на основе самособирающегося диагностического массива |
| JP7358388B2 (ja) | 2018-05-03 | 2023-10-10 | ベクトン・ディキンソン・アンド・カンパニー | 反対側の転写物末端における分子バーコーディング |
| CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
| WO2019213624A1 (en) * | 2018-05-04 | 2019-11-07 | The Regents Of The University Of California | Spiked primers for enrichment of pathogen nucleic acids among background of nucleic acids |
| ES2992135T3 (es) | 2018-10-01 | 2024-12-09 | Becton Dickinson Co | Determinar secuencias de transcripción 5 |
| US11932849B2 (en) | 2018-11-08 | 2024-03-19 | Becton, Dickinson And Company | Whole transcriptome analysis of single cells using random priming |
| US11492660B2 (en) | 2018-12-13 | 2022-11-08 | Becton, Dickinson And Company | Selective extension in single cell whole transcriptome analysis |
| US11371076B2 (en) | 2019-01-16 | 2022-06-28 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
| ES2945227T3 (es) | 2019-01-23 | 2023-06-29 | Becton Dickinson Co | Oligonucleótidos asociados con anticuerpos |
| WO2020167920A1 (en) | 2019-02-14 | 2020-08-20 | Cellular Research, Inc. | Hybrid targeted and whole transcriptome amplification |
| CN109852612A (zh) * | 2019-02-26 | 2019-06-07 | 上海翼和应用生物技术有限公司 | 一种结合通用荧光引物的发夹修饰引物 |
| US11965208B2 (en) | 2019-04-19 | 2024-04-23 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| EP4031683A4 (en) * | 2019-09-16 | 2024-01-17 | Exact Sciences Corporation | Structure and temperature-dependent flap endonuclease substrates |
| JP7522189B2 (ja) | 2019-11-08 | 2024-07-24 | ベクトン・ディキンソン・アンド・カンパニー | 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用 |
| WO2021146219A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Cell capture using du-containing oligonucleotides |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| ES3036514T3 (en) | 2020-01-16 | 2025-09-19 | Dnae Diagnostics Ltd | Compositions, kits and methods for isolating target polynucleotides |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| WO2021173719A1 (en) | 2020-02-25 | 2021-09-02 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| WO2021188186A2 (en) * | 2020-03-18 | 2021-09-23 | The Board Of Trustees Of The Leland Stanford Junior University | Detection of gene polymorphisms |
| US11661625B2 (en) | 2020-05-14 | 2023-05-30 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| US12391940B2 (en) | 2020-07-31 | 2025-08-19 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| KR20230098259A (ko) * | 2020-10-29 | 2023-07-03 | 비오까르띠 엔브이 | 멀티플렉스 핵산 검출을 위한 제네릭 카트리지 및 방법 |
| EP4247967A1 (en) | 2020-11-20 | 2023-09-27 | Becton, Dickinson and Company | Profiling of highly expressed and lowly expressed proteins |
| CN116685850A (zh) | 2020-12-15 | 2023-09-01 | 贝克顿迪金森公司 | 单细胞分泌组分析 |
| JP6972291B1 (ja) * | 2020-12-24 | 2021-11-24 | 栄研化学株式会社 | 核酸増幅方法、核酸増幅方法用プライマーセット、プローブ、及びキット |
| CN117683855B (zh) * | 2024-01-29 | 2024-05-17 | 杭州布平医学检验实验室有限公司 | 基因突变参考品的制备方法及其应用 |
Family Cites Families (139)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4284412A (en) | 1979-07-13 | 1981-08-18 | Ortho Diagnostics, Inc. | Method and apparatus for automated identification and enumeration of specified blood cell subclasses |
| US4498766A (en) | 1982-03-25 | 1985-02-12 | Becton, Dickinson And Company | Light beam focal spot elongation in flow cytometry devices |
| US4661913A (en) | 1984-09-11 | 1987-04-28 | Becton, Dickinson And Company | Apparatus and method for the detection and classification of articles using flow cytometry techniques |
| US4883750A (en) | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US4767206A (en) | 1984-12-24 | 1988-08-30 | Flow Cytometry Standards Corporation | Calibration method for flow cytometry using fluorescent microbeads and synthesis thereof |
| US4774189A (en) | 1984-12-24 | 1988-09-27 | Flow Cytometry Standards Corp. | Fluorescent calibration microbeads simulating stained cells |
| US4857451A (en) | 1984-12-24 | 1989-08-15 | Flow Cytometry Standards Corporation | Method of compensating and calibrating a flow cytometer, and microbead standards kit therefor |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4989977A (en) | 1985-07-29 | 1991-02-05 | Becton, Dickinson And Company | Flow cytometry apparatus with improved light beam adjustment |
| US4682195A (en) | 1985-09-30 | 1987-07-21 | General Electric Company | Insulated gate device with configured emitter contact pad |
| US4959463A (en) | 1985-10-15 | 1990-09-25 | Genentech, Inc. | Intermediates |
| US4659774A (en) | 1985-11-01 | 1987-04-21 | American Hoechst Corporation | Support for solid-phase oligonucleotide synthesis |
| US4714682A (en) | 1985-12-11 | 1987-12-22 | Flow Cytometry Standards Corporation | Fluorescent calibration microbeads simulating stained cells |
| ATE88761T1 (de) | 1986-01-10 | 1993-05-15 | Amoco Corp | Kompetitiver homogener test. |
| US4851331A (en) | 1986-05-16 | 1989-07-25 | Allied Corporation | Method and kit for polynucleotide assay including primer-dependant DNA polymerase |
| EP0266032A1 (en) | 1986-08-29 | 1988-05-04 | Beecham Group Plc | Modified fibrinolytic enzyme |
| AU622104B2 (en) | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
| US4816571A (en) | 1987-06-04 | 1989-03-28 | Applied Biosystems, Inc. | Chemical capping by phosphitylation during oligonucleotide synthesis |
| IL86724A (en) | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
| US4942124A (en) | 1987-08-11 | 1990-07-17 | President And Fellows Of Harvard College | Multiplex sequencing |
| CA1323293C (en) | 1987-12-11 | 1993-10-19 | Keith C. Backman | Assay using template-dependent nucleic acid probe reorganization |
| ATE92538T1 (de) | 1988-01-21 | 1993-08-15 | Genentech Inc | Verstaerkung und nachweis von nukleinsaeuresequenzen. |
| CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| IE61148B1 (en) | 1988-03-10 | 1994-10-05 | Ici Plc | Method of detecting nucleotide sequences |
| US5932413A (en) | 1988-04-01 | 1999-08-03 | Celebuski; Joseph Eugene | DNA probe assay using neutrally charged probe strands |
| US5602244A (en) | 1988-05-26 | 1997-02-11 | Competitive Technologies, Inc. | Polynucleotide phosphorodithioate compounds |
| US5858652A (en) | 1988-08-30 | 1999-01-12 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
| US5639611A (en) | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
| US4932207A (en) | 1988-12-28 | 1990-06-12 | Sundstrand Corporation | Segmented seal plate for a turbine engine |
| US5856092A (en) | 1989-02-13 | 1999-01-05 | Geneco Pty Ltd | Detection of a nucleic acid sequence or a change therein |
| US5141813A (en) | 1989-08-28 | 1992-08-25 | Clontech Laboratories, Inc. | Multifunctional controlled pore glass reagent for solid phase oligonucleotide synthesis |
| GB8920097D0 (en) | 1989-09-06 | 1989-10-18 | Ici Plc | Amplification processes |
| US5137806A (en) | 1989-12-11 | 1992-08-11 | Board Of Regents, The University Of Texas System | Methods and compositions for the detection of sequences in selected DNA molecules |
| US5160974A (en) | 1990-06-25 | 1992-11-03 | Flow Science, Inc. | Closed sample cell for use in flow cytometry |
| US5645987A (en) | 1990-09-21 | 1997-07-08 | Amgen Inc. | Enzymatic synthesis of oligonucleotides |
| US5478722A (en) | 1991-02-17 | 1995-12-26 | The Curators Of The University Of Missouri | Preserved cell preparations for flow cytometry and immunology |
| US5714331A (en) | 1991-05-24 | 1998-02-03 | Buchardt, Deceased; Ole | Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility |
| US5539082A (en) | 1993-04-26 | 1996-07-23 | Nielsen; Peter E. | Peptide nucleic acids |
| US5766855A (en) | 1991-05-24 | 1998-06-16 | Buchardt, Deceased; Ole | Peptide nucleic acids having enhanced binding affinity and sequence specificity |
| DK51092D0 (da) | 1991-05-24 | 1992-04-15 | Ole Buchardt | Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf |
| US5719262A (en) | 1993-11-22 | 1998-02-17 | Buchardt, Deceased; Ole | Peptide nucleic acids having amino acid side chains |
| US5846717A (en) | 1996-01-24 | 1998-12-08 | Third Wave Technologies, Inc. | Detection of nucleic acid sequences by invader-directed cleavage |
| US5846708A (en) | 1991-11-19 | 1998-12-08 | Massachusetts Institiute Of Technology | Optical and electrical methods and apparatus for molecule detection |
| DE4204650C1 (https=) | 1992-02-15 | 1993-07-08 | Hoffmeister, Helmut, Dr., 4400 Muenster, De | |
| EP0675966B1 (en) | 1992-02-19 | 2004-10-06 | The Public Health Research Institute Of The City Of New York, Inc. | Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids |
| DE69207580T2 (de) | 1992-03-23 | 1996-08-08 | Hoffmann La Roche | Verfahren zum DNS-Nachweis |
| JP3633932B2 (ja) | 1992-04-01 | 2005-03-30 | ザ ジョーンズ ホプキンズ ユニバーシティー スクール オブ メディシン | 糞便試料から単離した哺乳類の核酸を検出する方法、およびその検出用試薬 |
| US5428148A (en) | 1992-04-24 | 1995-06-27 | Beckman Instruments, Inc. | N4 - acylated cytidinyl compounds useful in oligonucleotide synthesis |
| GB9211979D0 (en) | 1992-06-05 | 1992-07-15 | Buchard Ole | Uses of nucleic acid analogues |
| US5843640A (en) | 1992-06-19 | 1998-12-01 | Northwestern University | Method of simultaneously detecting amplified nucleic acid sequences and cellular antigens in cells |
| US5861244A (en) | 1992-10-29 | 1999-01-19 | Profile Diagnostic Sciences, Inc. | Genetic sequence assay using DNA triple strand formation |
| US5658751A (en) | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| GB9311386D0 (en) | 1993-06-02 | 1993-07-21 | Pna Diagnostics As | Nucleic acid analogue assay procedures |
| US5846709A (en) | 1993-06-15 | 1998-12-08 | Imclone Systems Incorporated | Chemical process for amplifying and detecting nucleic acid sequences |
| US5837832A (en) | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
| FR2708288B1 (fr) | 1993-07-26 | 1995-09-01 | Bio Merieux | Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé. |
| US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| CA2137728C (en) | 1993-12-14 | 1998-07-14 | William E. Keating | Enzymatic removal of non-target nucleic acid from biological samples |
| DE69432919T2 (de) | 1993-12-28 | 2004-05-27 | Tanabe Seiyaku Co., Ltd. | Verfahren für den Nachweis spezifischer Polynukleotiden |
| US5928905A (en) | 1995-04-18 | 1999-07-27 | Glaxo Group Limited | End-complementary polymerase reaction |
| ATE220424T1 (de) | 1994-05-28 | 2002-07-15 | Tepnel Medical Ltd | Herstellung von nukleinsaeurekopien |
| US5942391A (en) | 1994-06-22 | 1999-08-24 | Mount Sinai School Of Medicine | Nucleic acid amplification method: ramification-extension amplification method (RAM) |
| DE69528706T2 (de) | 1994-08-19 | 2003-06-12 | Pe Corp. (Ny), Foster City | Gekoppeltes ampflikation- und ligationverfahren |
| US5574146A (en) | 1994-08-30 | 1996-11-12 | Beckman Instruments, Inc. | Oligonucleotide synthesis with substituted aryl carboxylic acids as activators |
| US5599668A (en) | 1994-09-22 | 1997-02-04 | Abbott Laboratories | Light scattering optical waveguide method for detecting specific binding events |
| US5554744A (en) | 1994-09-23 | 1996-09-10 | Hybridon, Inc. | Method for loading solid supports for nucleic acid synthesis |
| US5604097A (en) | 1994-10-13 | 1997-02-18 | Spectragen, Inc. | Methods for sorting polynucleotides using oligonucleotide tags |
| DE69535240T2 (de) | 1994-10-28 | 2007-06-06 | Gen-Probe Inc., San Diego | Zusammensetzungen und Verfahren für die gleichzeitige Detektion und Quantifizierung von einer Mehrheit spezifischer Nuklein Säure Sequenzen |
| FR2726277B1 (fr) * | 1994-10-28 | 1996-12-27 | Bio Merieux | Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin |
| US5935825A (en) | 1994-11-18 | 1999-08-10 | Shimadzu Corporation | Process and reagent for amplifying nucleic acid sequences |
| US5843650A (en) | 1995-05-01 | 1998-12-01 | Segev; David | Nucleic acid detection and amplification by chemical linkage of oligonucleotides |
| US5929227A (en) | 1995-07-12 | 1999-07-27 | The Regents Of The University Of California | Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores |
| EP0951541B1 (en) | 1995-07-31 | 2005-11-30 | Urocor, Inc. | Biomarkers and targets for diagnosis, prognosis and management of prostate disease |
| US5916779A (en) | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
| US5981180A (en) | 1995-10-11 | 1999-11-09 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and methods |
| US5736330A (en) | 1995-10-11 | 1998-04-07 | Luminex Corporation | Method and compositions for flow cytometric determination of DNA sequences |
| US5866331A (en) | 1995-10-20 | 1999-02-02 | University Of Massachusetts | Single molecule detection by in situ hybridization |
| US5705629A (en) | 1995-10-20 | 1998-01-06 | Hybridon, Inc. | Methods for H-phosphonate synthesis of mono- and oligonucleotides |
| US5612473A (en) | 1996-01-16 | 1997-03-18 | Gull Laboratories | Methods, kits and solutions for preparing sample material for nucleic acid amplification |
| EP2332957B1 (en) | 1996-02-09 | 2015-04-08 | Cornell Research Foundation, Inc. | Detection of nucleic and acid sequence differences using the ligase detection reaction with addressable arrays |
| US5928906A (en) | 1996-05-09 | 1999-07-27 | Sequenom, Inc. | Process for direct sequencing during template amplification |
| US5912124A (en) | 1996-06-14 | 1999-06-15 | Sarnoff Corporation | Padlock probe detection |
| US5939291A (en) | 1996-06-14 | 1999-08-17 | Sarnoff Corporation | Microfluidic method for nucleic acid amplification |
| US5866336A (en) | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US6117635A (en) | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US5853990A (en) | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
| US5849546A (en) | 1996-09-13 | 1998-12-15 | Epicentre Technologies Corporation | Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates |
| US5853992A (en) | 1996-10-04 | 1998-12-29 | The Regents Of The University Of California | Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels |
| US5853993A (en) | 1996-10-21 | 1998-12-29 | Hewlett-Packard Company | Signal enhancement method and kit |
| US5908845A (en) | 1996-10-30 | 1999-06-01 | Segev; David | Polyether nucleic acids |
| US5905024A (en) | 1996-12-17 | 1999-05-18 | University Of Chicago | Method for performing site-specific affinity fractionation for use in DNA sequencing |
| US5846729A (en) | 1997-02-27 | 1998-12-08 | Lorne Park Research, Inc. | Assaying nucleotides in solution using a fluorescent intensity quenching effect |
| US5837860A (en) | 1997-03-05 | 1998-11-17 | Molecular Tool, Inc. | Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds |
| US5849497A (en) | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
| US5846726A (en) | 1997-05-13 | 1998-12-08 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| US5928869A (en) | 1997-05-30 | 1999-07-27 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| US5866366A (en) | 1997-07-01 | 1999-02-02 | Smithkline Beecham Corporation | gidB |
| US5916776A (en) | 1997-08-27 | 1999-06-29 | Sarnoff Corporation | Amplification method for a polynucleotide |
| US5935791A (en) | 1997-09-23 | 1999-08-10 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| US5932451A (en) | 1997-11-19 | 1999-08-03 | Incyte Pharmaceuticals, Inc. | Method for unbiased mRNA amplification |
| GB9812768D0 (en) * | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
| GB9902971D0 (en) | 1999-02-11 | 1999-03-31 | Zeneca Ltd | Assay |
| US6964847B1 (en) * | 1999-07-14 | 2005-11-15 | Packard Biosciences Company | Derivative nucleic acids and uses thereof |
| US6287778B1 (en) | 1999-10-19 | 2001-09-11 | Affymetrix, Inc. | Allele detection using primer extension with sequence-coded identity tags |
| US7838225B2 (en) | 1999-10-29 | 2010-11-23 | Hologic, Inc. | Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase |
| DK1259643T3 (da) * | 2000-02-07 | 2009-02-23 | Illumina Inc | Fremgangsmåde til detektion af nukleinsyre ved anvendelse af universel priming |
| US20050106564A1 (en) | 2000-06-02 | 2005-05-19 | Hechinger Mark K. | One-step, no-wash multiplex bead-based flow cytometric assay |
| WO2001094625A2 (en) | 2000-06-06 | 2001-12-13 | Tm Bioscience Corporation | Capture moieties for nucleic acids and uses thereof |
| ES2382542T3 (es) | 2001-01-25 | 2012-06-11 | Luminex Molecular Diagnostics, Inc. | Polinucleótidos para su utilización como etiquetas y complementos de etiqueta, fabricación y utilización de los mismos |
| US7226737B2 (en) | 2001-01-25 | 2007-06-05 | Luminex Molecular Diagnostics, Inc. | Polynucleotides for use as tags and tag complements, manufacture and use thereof |
| EP1439222A1 (en) | 2001-10-26 | 2004-07-21 | Matsushita Electric Industrial Co., Ltd. | Method of detecting target nucleic acid and nucleic acid probe |
| US6803201B2 (en) | 2002-01-24 | 2004-10-12 | Stratagene | Compositions and methods for polynucleotide sequence determination |
| JP2004205414A (ja) | 2002-12-26 | 2004-07-22 | Institute Of Physical & Chemical Research | 核酸マイクロアレイ、その作製方法及びその使用方法 |
| US7700750B2 (en) * | 2003-01-09 | 2010-04-20 | Third Wave Technologies, Inc. | Connexin allele detection assays |
| JP2004283024A (ja) | 2003-03-19 | 2004-10-14 | Junsei Chemical Co Ltd | 新規アンチセンスオリゴヌクレオチド及び抗ｈｉｖ剤 |
| NZ544959A (en) | 2003-07-07 | 2009-02-28 | One Cell Systems Inc | Hairpin-labeled probes and methods of use |
| US20070092880A1 (en) | 2003-07-16 | 2007-04-26 | Crothers Donald M | Invasive cleavage reaction with electrochemical readout |
| US20070099228A1 (en) * | 2004-11-04 | 2007-05-03 | Applera Corporation | Concatameric Ligation Products: Compositions, Methods and Kits for Same |
| WO2005047533A1 (en) | 2003-11-17 | 2005-05-26 | Tm Bioscience Corporation | Method of detecting mutations associated with thrombosis |
| JP2005253427A (ja) * | 2004-03-15 | 2005-09-22 | Aisin Seiki Co Ltd | 核酸検出方法及び核酸単離方法 |
| CA2479968A1 (en) | 2004-09-01 | 2006-03-01 | Keith R. Walley | Protein c and endothelial protein c receptor polymorphisms as indicators of patient outcome |
| ES2292270B1 (es) | 2004-04-14 | 2009-02-16 | Oryzon Genomics, S.A. | Procedimiento para detectar selectivamente acidos nucleicos con estructuras atipicas convertibles en muescas. |
| CA2568499A1 (en) | 2004-06-01 | 2005-12-15 | Tm Bioscience Corporation | Method of detecting cystic fibrosis associated mutations |
| ATE471991T1 (de) | 2004-06-30 | 2010-07-15 | Luminex Molecular Diagnostics | Verfahren zum nachweis von mutationen im für cytochrom p450-2d6 codierenden gen |
| CA2571817A1 (en) | 2004-06-30 | 2006-01-12 | Tm Bioscience Pgx, Inc. | Method of detecting mutations in the gene encoding cytochrome p450-2c9 |
| WO2006071770A2 (en) * | 2004-12-23 | 2006-07-06 | I-Stat Corporation | Molecular diagnostics system and methods |
| US20070099211A1 (en) * | 2005-07-15 | 2007-05-03 | Vissarion Aivazachvili | Detection of nucleic acid amplification |
| MX2008009654A (es) | 2006-01-24 | 2009-01-15 | Univ British Columbia | Polimorfismos de vias de vasopresina como indicadores del resultado expuesto en sujetos criticamente enfermos. |
| US20070172841A1 (en) | 2006-01-25 | 2007-07-26 | Hui Wang | Probe/target stabilization with add-in oligo |
| JP2008048648A (ja) | 2006-08-23 | 2008-03-06 | Institute Of Physical & Chemical Research | 核酸増幅用プライマーセット及び核酸の増幅方法 |
| EP2079754B1 (en) | 2006-10-04 | 2017-03-15 | Third Wave Technologies, Inc. | Snap-back primers and detectable hairpin structures |
| CN102851369B (zh) | 2006-12-13 | 2015-01-21 | 卢米耐克斯公司 | 用于实时pcr多重分析的系统和方法 |
| US7862999B2 (en) * | 2007-01-17 | 2011-01-04 | Affymetrix, Inc. | Multiplex targeted amplification using flap nuclease |
| EP2167964B1 (de) | 2007-06-13 | 2015-03-11 | Attomol GmbH Molekulare Diagnostika | Verfahren zur durchführung und auswertung von mix&measure assays für die messung von reaktionskinetiken sowie von konzentrationen und affinitäten von analyten im multiplexformat |
| EP2215245A2 (en) | 2007-11-02 | 2010-08-11 | Luminex Molecular Diagnostics, Inc. | One-step target detection assay |
| US8008019B2 (en) * | 2007-11-28 | 2011-08-30 | Luminex Molecular Diagnostics | Use of dual-tags for the evaluation of genomic variable repeat regions |
| JP4744503B2 (ja) | 2007-11-30 | 2011-08-10 | シャープ株式会社 | 操作処理装置 |
| US8312249B1 (en) | 2008-10-10 | 2012-11-13 | Apple Inc. | Dynamic trampoline and structured code generation in a signed code environment |
| FR2957821B1 (fr) | 2010-03-24 | 2014-08-29 | Inst Francais Du Petrole | Nouvelle zone de regeneration du catalyseur divisee en secteurs pour unites catalytiques regeneratives |
-
2010
- 2010-06-29 US US12/826,189 patent/US9587270B2/en active Active
- 2010-06-29 WO PCT/US2010/040354 patent/WO2011008530A2/en not_active Ceased
- 2010-06-29 EP EP10800291.6A patent/EP2449104B1/en active Active
- 2010-06-29 CN CN201080035685.3A patent/CN102686728B/zh active Active
- 2010-06-29 EP EP14168543.8A patent/EP2789689B1/en active Active
- 2010-06-29 JP JP2012518572A patent/JP5785942B2/ja active Active
- 2010-06-29 CA CA2766351A patent/CA2766351C/en active Active
-
2014
- 2014-05-06 US US14/270,785 patent/US10577647B2/en active Active
-
2015
- 2015-07-27 JP JP2015147338A patent/JP6270786B2/ja active Active
-
2017
- 2017-12-26 JP JP2017248991A patent/JP6591521B2/ja active Active
-
2020
- 2020-01-23 US US16/750,319 patent/US11319577B2/en active Active
-
2022
- 2022-03-31 US US17/709,693 patent/US11884965B2/en active Active
-
2023
- 2023-12-22 US US18/394,469 patent/US12473586B2/en active Active
-
2025
- 2025-10-24 US US19/368,597 patent/US20260103744A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP2789689A1 (en) | 2014-10-15 |
| WO2011008530A2 (en) | 2011-01-20 |
| US11884965B2 (en) | 2024-01-30 |
| CA2766351A1 (en) | 2011-01-20 |
| US20140242586A1 (en) | 2014-08-28 |
| CN102686728A (zh) | 2012-09-19 |
| US11319577B2 (en) | 2022-05-03 |
| JP6270786B2 (ja) | 2018-01-31 |
| EP2449104A4 (en) | 2012-11-07 |
| US9587270B2 (en) | 2017-03-07 |
| WO2011008530A3 (en) | 2011-05-05 |
| US12473586B2 (en) | 2025-11-18 |
| US10577647B2 (en) | 2020-03-03 |
| JP6591521B2 (ja) | 2019-10-16 |
| EP2449104B1 (en) | 2014-06-04 |
| US20100330574A1 (en) | 2010-12-30 |
| JP2018042577A (ja) | 2018-03-22 |
| JP2015226542A (ja) | 2015-12-17 |
| US20260103744A1 (en) | 2026-04-16 |
| US20220251633A1 (en) | 2022-08-11 |
| JP5785942B2 (ja) | 2015-09-30 |
| EP2789689B1 (en) | 2016-04-27 |
| EP2449104A2 (en) | 2012-05-09 |
| JP2012531913A (ja) | 2012-12-13 |
| US20200149093A1 (en) | 2020-05-14 |
| CN102686728B (zh) | 2018-09-18 |
| US20240318233A1 (en) | 2024-09-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US12473586B2 (en) | Chimeric primers with hairpin conformations and methods of using same | |
| US11371082B2 (en) | Cleavable hairpin primers | |
| US20140017689A1 (en) | Method for detecting nucleic acids | |
| JP2004511227A (ja) | 核酸の同種内検出用の特異的二本鎖プローブおよびその適用方法 | |
| US20090148849A1 (en) | One-step target detection assay | |
| WO2016007914A1 (en) | Dna amplification technology | |
| EP3055430B1 (en) | Method for the detection of target nucleic acid sequences | |
| WO2018139955A1 (ru) | Способ специфической идентификации последовательностей днк | |
| HK1174946A (en) | Chimeric primers with hairpin conformations and methods of using same | |
| HK1174946B (zh) | 具有发夹构象的嵌合引物及其使用方法 | |
| HK1227444B (en) | Method for the detection of target nucleic acid sequences | |
| HK1227444A1 (en) | Method for the detection of target nucleic acid sequences |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20150211 |
|
| MPN | Maintenance fee for patent paid |
Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 15TH ANNIV.) - STANDARD Year of fee payment: 15 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20250620 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20250620 |