EA202091646A1 - Платформа на основе самособирающегося диагностического массива - Google Patents

Платформа на основе самособирающегося диагностического массива

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Publication number
EA202091646A1
EA202091646A1 EA202091646A EA202091646A EA202091646A1 EA 202091646 A1 EA202091646 A1 EA 202091646A1 EA 202091646 A EA202091646 A EA 202091646A EA 202091646 A EA202091646 A EA 202091646A EA 202091646 A1 EA202091646 A1 EA 202091646A1
Authority
EA
Eurasian Patent Office
Prior art keywords
nucleic acid
present
self
primer
platform based
Prior art date
Application number
EA202091646A
Other languages
English (en)
Inventor
Роберт Боухеннон
Марк Стюарт
Дэвид Робсон
Эдвард Фаррелл
Линда Хендерсон
Натан Макауэн
Севен Боухеннон
Original Assignee
Квошент Сюисс Са
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Квошент Сюисс Са filed Critical Квошент Сюисс Са
Publication of EA202091646A1 publication Critical patent/EA202091646A1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Настоящее изобретение относится к способам и наборам для детектирования нуклеиновой кислоты или антигена в образце с использованием платформы на основе универсального массива. Так, например, нуклеиновая кислота может быть детектирована путем амплификации по меньшей мере части нуклеиновой кислоты из образца с использованием пары праймеров, включающей первый праймер, содержащий метку, и первую олигонуклеотидную последовательность, которая гибридизуется с первой цепью части нуклеиновой кислоты, и второй праймер, содержащий вторую олигонуклеотидную последовательность, которая гибридизуется со второй цепью части нуклеиновой кислоты; контактирование ампликона, если он присутствует, со множеством одноцепочечных олигонуклеотидных последовательностей для захвата, каждая из которых присоединена к твердому носителю; нанесение коллоидного детектирующего реагента на твердые носители; промывку твердых носителей промывочным раствором и детектирование коллоидного детектирующего реагента. Настоящее изобретение также относится к специфическим олигонуклеотидным последовательностям для захвата и связывания.
EA202091646A 2018-01-05 2018-12-19 Платформа на основе самособирающегося диагностического массива EA202091646A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862614313P 2018-01-05 2018-01-05
PCT/EP2018/085945 WO2019134835A1 (en) 2018-01-05 2018-12-19 Self-assembling diagnostic array platform

Publications (1)

Publication Number Publication Date
EA202091646A1 true EA202091646A1 (ru) 2020-11-17

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EA202091646A EA202091646A1 (ru) 2018-01-05 2018-12-19 Платформа на основе самособирающегося диагностического массива

Country Status (8)

Country Link
US (1) US20200385792A1 (ru)
EP (1) EP3735473A1 (ru)
JP (2) JP2021510085A (ru)
CN (1) CN111819289A (ru)
AU (1) AU2018400335A1 (ru)
CA (1) CA3087624A1 (ru)
EA (1) EA202091646A1 (ru)
WO (1) WO2019134835A1 (ru)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3044782A1 (en) 2017-12-29 2019-06-29 Clear Labs, Inc. Automated priming and library loading device
JP2022546443A (ja) * 2019-08-27 2022-11-04 エフ．ホフマン－ラ ロシュ アーゲー ｃｃｃＤＮＡから転写されたＨＢＶ ＲＮＡを含む、Ｂ型肝炎ウイルスＲＮＡの増幅および検出のための組成物および方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9404709D0 (en) * 1994-03-11 1994-04-27 Multilyte Ltd Binding assay
US20010031468A1 (en) * 2000-02-08 2001-10-18 Alex Chenchik Analyte assays employing universal arrays
DE10046184A1 (de) * 2000-09-18 2002-04-04 November Ag Molekulare Medizin Verfahren zum Nachweis mindestens einer Nukleinsäuresequenz
FR2823438B1 (fr) * 2001-04-11 2004-09-17 Nuxe Lab Emulsion a base d'alcools gras et de glucolipides a stabilite amelioree et viscosite elevee, utilisable en cosmetique
US20040161741A1 (en) * 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US7132233B2 (en) * 2002-12-12 2006-11-07 Novartis Vaccines And Diagnostics, Inc. Identification of oligonucleotides for the capture, detection and quantitation of West Nile virus
US20050191636A1 (en) * 2004-03-01 2005-09-01 Biocept, Inc. Detection of STRP, such as fragile X syndrome
US8980561B1 (en) * 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
US20090148849A1 (en) * 2007-11-02 2009-06-11 Barbara Galvan-Goldman One-step target detection assay
WO2011008530A2 (en) * 2009-06-29 2011-01-20 Luminex Corporation Chimeric primers with hairpin conformations and methods of using same
EP2566981A2 (en) * 2010-05-07 2013-03-13 Quantibact A/s Method for generating a double stranded nucleic acid with a single stranded overhang
WO2013039228A1 (ja) * 2011-09-14 2013-03-21 日本碍子株式会社 標的核酸の検出方法
US9783844B2 (en) * 2012-04-27 2017-10-10 Kaneka Corporation Method for amplifying nucleic acid and method for detecting amplified nucleic acid
DK2972353T3 (da) * 2013-03-11 2023-03-27 Meso Scale Technologies Llc Forbedrede fremgangsmåder til udførelse af multipleksanalyser
WO2016099999A1 (en) * 2014-12-18 2016-06-23 Ge Healthcare Uk Limited Analyte detection on a solid support by nucleic acid amplification coupled to an immunoassay
US11060131B2 (en) * 2015-03-25 2021-07-13 Angle Europe Limited Solid phase nucleic acid target capture and replication using strand displacing polymerases

Also Published As

Publication number Publication date
JP2023110042A (ja) 2023-08-08
AU2018400335A1 (en) 2020-07-02
WO2019134835A1 (en) 2019-07-11
EP3735473A1 (en) 2020-11-11
CA3087624A1 (en) 2019-07-11
CN111819289A (zh) 2020-10-23
JP2021510085A (ja) 2021-04-15
US20200385792A1 (en) 2020-12-10

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