EA201792628A2 - Способ специфической идентификации последовательностей днк - Google Patents

Способ специфической идентификации последовательностей днк

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Publication number
EA201792628A2
EA201792628A2 EA201792628A EA201792628A EA201792628A2 EA 201792628 A2 EA201792628 A2 EA 201792628A2 EA 201792628 A EA201792628 A EA 201792628A EA 201792628 A EA201792628 A EA 201792628A EA 201792628 A2 EA201792628 A2 EA 201792628A2
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EA
Eurasian Patent Office
Prior art keywords
mda
specific identification
dna sequence
dna sequences
multiplex
Prior art date
Application number
EA201792628A
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English (en)
Other versions
EA201792628A3 (ru
EA035333B1 (ru
Inventor
Виталий Евгеньевич ВЕДЕРНИКОВ
Original Assignee
Общество с ограниченной ответственностью "Секвойя Дженетикс"
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Application filed by Общество с ограниченной ответственностью "Секвойя Дженетикс" filed Critical Общество с ограниченной ответственностью "Секвойя Дженетикс"
Publication of EA201792628A2 publication Critical patent/EA201792628A2/ru
Publication of EA201792628A3 publication Critical patent/EA201792628A3/ru
Publication of EA035333B1 publication Critical patent/EA035333B1/ru

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Изобретение относится к области молекулярной биологии и может быть использовано в клинической диагностике in vitro. Для этого предложен способ специфической идентификации последовательностей ДНК - мультиплексный дуплекс-опосредованный анализ (МДА), осуществляемый посредством проведения полимеразной цепной реакции в присутствии зондов, имеющих оригинальную конструкцию (фиг. 1). МДА позволяет специфично определять последовательности ДНК, в том числе в мультиплексном формате, с возможностью определения до десяти мишеней в одном канале амплификатора. При проведении МДА достигается высокая степень мультиплексирования с достижением высокой чувствительности и специфичности метода. Изобретение также может быть использовано для определения нуклеотидных полиморфизмов.
EA201792628A 2017-01-25 2017-12-26 Способ специфической идентификации последовательностей днк EA035333B1 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2017102365A RU2665631C2 (ru) 2017-01-25 2017-01-25 Способ специфической идентификации последовательностей днк

Publications (3)

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EA201792628A2 true EA201792628A2 (ru) 2018-07-31
EA201792628A3 EA201792628A3 (ru) 2018-08-31
EA035333B1 EA035333B1 (ru) 2020-05-28

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Country Status (4)

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EP (1) EP3575406A4 (ru)
EA (1) EA035333B1 (ru)
RU (1) RU2665631C2 (ru)
WO (1) WO2018139955A1 (ru)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2707949C1 (ru) * 2018-12-03 2019-12-02 Закрытое акционерное общество "Синтол" (ЗАО "Синтол") Многоканальный капиллярный генетический анализатор
CN114540345B (zh) * 2021-11-03 2024-04-09 武汉蓝沙医学检验实验室有限公司 一种发夹结构的标签荧光探针和荧光检测方法

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US43517A (en) 1864-07-12 Improvement in water-elevators
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US5541311A (en) 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
GB9803382D0 (en) * 1998-02-19 1998-04-15 Secr Defence Detection system
US7381532B2 (en) 1999-10-29 2008-06-03 Stratagene California Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US6893819B1 (en) 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US8076067B2 (en) * 2006-08-15 2011-12-13 Genetag Technology, Inc. Probe-antiprobe compositions and methods for DNA or RNA detection
US20080241838A1 (en) 2006-12-29 2008-10-02 Applera Corporation, Applied Biosystems Group Methods and systems for detecting nucleic acids
CA2735246C (en) * 2008-07-30 2015-02-24 Nippon Steel Kankyo Engineering Co., Ltd. Nucleic acid probe set and method of using the same
WO2010013017A1 (en) * 2008-07-31 2010-02-04 Oxitec Limited Multiplex amplification and detection
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WO2012096430A1 (en) * 2011-01-11 2012-07-19 Seegene, Inc. Detection of target nucleic acid sequences by pto cleavage and extension assay
GB201106254D0 (en) * 2011-04-13 2011-05-25 Frisen Jonas Method and product
KR102468174B1 (ko) * 2016-09-15 2022-11-17 에프. 호프만-라 로슈 아게 멀티플렉스 pcr 수행 방법

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Publication number Publication date
EA201792628A3 (ru) 2018-08-31
EP3575406A4 (en) 2021-02-24
WO2018139955A1 (ru) 2018-08-02
RU2665631C2 (ru) 2018-09-03
RU2017102365A3 (ru) 2018-07-26
RU2017102365A (ru) 2018-07-26
EP3575406A1 (en) 2019-12-04
EA035333B1 (ru) 2020-05-28

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