HUP0301031A2 - Degradálható nukleinsavpróbák, és eljárások nukleinsavak kimutatására - Google Patents

Degradálható nukleinsavpróbák, és eljárások nukleinsavak kimutatására

Info

Publication number
HUP0301031A2
HUP0301031A2 HU0301031A HUP0301031A HUP0301031A2 HU P0301031 A2 HUP0301031 A2 HU P0301031A2 HU 0301031 A HU0301031 A HU 0301031A HU P0301031 A HUP0301031 A HU P0301031A HU P0301031 A2 HUP0301031 A2 HU P0301031A2
Authority
HU
Hungary
Prior art keywords
nucleic acid
probes
region
degradable
detection methods
Prior art date
Application number
HU0301031A
Other languages
English (en)
Inventor
David Albagli
Peter C. Cheng
Reuel Vanatta
Michael L. Wood
Original Assignee
Naxcor
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Naxcor filed Critical Naxcor
Publication of HUP0301031A2 publication Critical patent/HUP0301031A2/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A találmány tárgyát enzimatikus degradációra érzékeny nukleinsavpróbákképezik, valamint a találmány szerinti próbák alkalmazásán alapulóeljárások, célnukleinsav-szekvenciák kimutatására különböző mintákban.Közelebbről, a találmány tárgyát olyan nukleinsavpróbák ésnukleinsavpróba párok képezik, amelyek célspecifikus hibridizációsrégiója vagy célszekvenciával komplementer régiója a kimutathatórégióról degradációs eljárással leválasztható. A próbák megmaradórégiója egyszerűen kimutatható. A degradálható próbák alkalmazásával anukleinsav kimutatási eljárásokban jelentősen csökkenthető aháttérspecifikus vagy nemspecifikus jel keletkezése, és ezáltaljavítható a jel-zaj viszony. Ó
HU0301031A 2000-04-18 2001-04-18 Degradálható nukleinsavpróbák, és eljárások nukleinsavak kimutatására HUP0301031A2 (hu)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/551,305 US6573048B1 (en) 2000-04-18 2000-04-18 Degradable nucleic acid probes and nucleic acid detection methods
PCT/US2001/012701 WO2001079563A2 (en) 2000-04-18 2001-04-18 Degradable nucleic acid probes and nucleic acid detection methods

Publications (1)

Publication Number Publication Date
HUP0301031A2 true HUP0301031A2 (hu) 2003-07-28

Family

ID=24200719

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0301031A HUP0301031A2 (hu) 2000-04-18 2001-04-18 Degradálható nukleinsavpróbák, és eljárások nukleinsavak kimutatására

Country Status (11)

Country Link
US (1) US6573048B1 (hu)
EP (1) EP1280938A2 (hu)
JP (1) JP2004501615A (hu)
KR (1) KR20030005289A (hu)
CN (1) CN1432069A (hu)
CA (1) CA2406096A1 (hu)
CZ (1) CZ20023749A3 (hu)
HU (1) HUP0301031A2 (hu)
MX (1) MXPA02010283A (hu)
PL (1) PL362928A1 (hu)
WO (1) WO2001079563A2 (hu)

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EP1513958B1 (en) 2002-06-14 2011-08-10 Gen-Probe Incorporated Compositions for detecting hepatitis b virus
US8575327B2 (en) * 2003-06-12 2013-11-05 Alnylam Pharmaceuticals, Inc. Conserved HBV and HCV sequences useful for gene silencing
EP2471924A1 (en) 2004-05-28 2012-07-04 Asuragen, INC. Methods and compositions involving microRNA
US20060078894A1 (en) * 2004-10-12 2006-04-13 Winkler Matthew M Methods and compositions for analyzing nucleic acids
EP2302055B1 (en) * 2004-11-12 2014-08-27 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
EP1956089B1 (en) * 2005-11-17 2012-02-08 Japan Science and Technology Agency Method for detecting target nucleic acid with specific base sequence and set of nucleic acids for detection
US20080131878A1 (en) * 2006-12-05 2008-06-05 Asuragen, Inc. Compositions and Methods for the Detection of Small RNA
WO2008073915A2 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Micrornas differentially expressed in leukemia and uses thereof
AU2007333106A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. miR-20 regulated genes and pathways as targets for therapeutic intervention
CA2671270A1 (en) * 2006-12-29 2008-07-17 Asuragen, Inc. Mir-16 regulated genes and pathways as targets for therapeutic intervention
US20090232893A1 (en) * 2007-05-22 2009-09-17 Bader Andreas G miR-143 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
WO2008154333A2 (en) * 2007-06-08 2008-12-18 Asuragen, Inc. Mir-34 regulated genes and pathways as targets for therapeutic intervention
EP2198050A1 (en) * 2007-09-14 2010-06-23 Asuragen, INC. Micrornas differentially expressed in cervical cancer and uses thereof
WO2009052386A1 (en) * 2007-10-18 2009-04-23 Asuragen, Inc. Micrornas differentially expressed in lung diseases and uses thereof
WO2009070805A2 (en) 2007-12-01 2009-06-04 Asuragen, Inc. Mir-124 regulated genes and pathways as targets for therapeutic intervention
WO2009126726A1 (en) * 2008-04-08 2009-10-15 Asuragen, Inc Methods and compositions for diagnosing and modulating human papillomavirus (hpv)
US8258111B2 (en) * 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
EP2358898B1 (en) * 2008-11-17 2016-08-31 Headway Technologies, Inc. Methods and compositions in particle-based detection of target molecules using covalent bond forming reactive pairs
US9150910B2 (en) * 2008-11-17 2015-10-06 Headway Technologies, Inc. Methods and compositions in particle-based detection of target molecules using linking molecules
US9447455B2 (en) 2011-02-16 2016-09-20 Headway Technologies, Inc. Methods and compositions for the target-localized anchoring of detectable label
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
CN102703584B (zh) * 2012-04-20 2013-08-21 中国人民解放军第四军医大学 自组装聚合分支dna探针的制备方法
US9738925B2 (en) * 2013-03-15 2017-08-22 Lyle J. Arnold Methods for immobilizing target nucleic acids utilizing combinatorial capture probes
JP6210484B2 (ja) * 2013-06-25 2017-10-11 国立大学法人北陸先端科学技術大学院大学 鎖交換された二重鎖オリゴヌクレオチドの製造方法
GB201401885D0 (en) * 2014-02-04 2014-03-19 Olink Ab Proximity assay with detection based on hybridisation chain reaction (HCR)
JOP20200092A1 (ar) 2014-11-10 2017-06-16 Alnylam Pharmaceuticals Inc تركيبات iRNA لفيروس الكبد B (HBV) وطرق لاستخدامها
EP3507364A4 (en) * 2016-08-31 2020-05-20 President and Fellows of Harvard College METHODS OF GENERATING NUCLEIC ACID SEQUENCE LIBRARIES FOR IN SITU FLUORESCENT SEQUENCING DETECTION
US11324820B2 (en) 2017-04-18 2022-05-10 Alnylam Pharmaceuticals, Inc. Methods for the treatment of subjects having a hepatitis b virus (HBV) infection
MX2021001056A (es) 2018-08-13 2021-04-12 Alnylam Pharmaceuticals Inc Composiciones de agente de acido ribonucleico bicatenario (arnbc) de virus de la hepatitis b (vhb) y metodos de uso de las mismas.
CN110295235A (zh) * 2019-07-26 2019-10-01 合肥欧创基因生物科技有限公司 一种双标记探针及其在snp分型及肿瘤突变的应用

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US4826967A (en) 1987-06-16 1989-05-02 Naxcor Psoralen-nucleoside adducts and method for their preparation
US5403711A (en) * 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
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US6124099A (en) * 1998-06-22 2000-09-26 The University Of Vermont And State Agricultural College Method for placing a photo-cross-linking agent at specific internal sites within the sequence of synthetic strands of ribonucleic acids
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Also Published As

Publication number Publication date
WO2001079563A2 (en) 2001-10-25
KR20030005289A (ko) 2003-01-17
CN1432069A (zh) 2003-07-23
WO2001079563A3 (en) 2002-09-19
US6573048B1 (en) 2003-06-03
JP2004501615A (ja) 2004-01-22
PL362928A1 (en) 2004-11-02
EP1280938A2 (en) 2003-02-05
CZ20023749A3 (cs) 2003-04-16
MXPA02010283A (es) 2003-05-23
CA2406096A1 (en) 2001-10-25

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