MX2019011503A - Composiciones de cebador y métodos multiplex para detectar la presencia de listeria. - Google Patents

Composiciones de cebador y métodos multiplex para detectar la presencia de listeria.

Info

Publication number
MX2019011503A
MX2019011503A MX2019011503A MX2019011503A MX2019011503A MX 2019011503 A MX2019011503 A MX 2019011503A MX 2019011503 A MX2019011503 A MX 2019011503A MX 2019011503 A MX2019011503 A MX 2019011503A MX 2019011503 A MX2019011503 A MX 2019011503A
Authority
MX
Mexico
Prior art keywords
listeria
primer
detecting
primer sequence
additional
Prior art date
Application number
MX2019011503A
Other languages
English (en)
Inventor
Ziebell Bradley
Sweeney Kari
Akins-Lewenthal Deann
Original Assignee
Conagra Foods Rdm Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Conagra Foods Rdm Inc filed Critical Conagra Foods Rdm Inc
Publication of MX2019011503A publication Critical patent/MX2019011503A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Los aspectos de la divulgación se refieren a composiciones de cebador y métodos multiplex para detectar la presencia de Listeria. La composición de cebador incluye una primera secuencia de cebador operable para amplificar un primer producto de reacción en cadena de la polimerasa (PCR) (p.ej., un ácido nucleico, un fragmento del mismo, un complemento del mismo, o un gen). La primera secuencia de cebador es específica para el género Listeria. La composición de cebador también incluye al menos una secuencia de cebador adicional operable para amplificar al menos un producto de PCR adicional. La(s) secuencia(s) de cebador adicional(es) es/son específica(s) para una o más especies o subespecies de Listeria. El método multiplex incluye poner en contacto una muestra con la composición de cebador para detectar sustancialmente de manera simultánea la presencia del género Listeria y diferenciar entre especies de Listeria presentes en la muestra.
MX2019011503A 2017-03-31 2018-03-30 Composiciones de cebador y métodos multiplex para detectar la presencia de listeria. MX2019011503A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762479695P 2017-03-31 2017-03-31
US201762526584P 2017-06-29 2017-06-29
PCT/US2018/025425 WO2018183865A1 (en) 2017-03-31 2018-03-30 Primer compositions and multiplex methods for detecting the presence of listeria

Publications (1)

Publication Number Publication Date
MX2019011503A true MX2019011503A (es) 2019-12-19

Family

ID=63677046

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019011503A MX2019011503A (es) 2017-03-31 2018-03-30 Composiciones de cebador y métodos multiplex para detectar la presencia de listeria.

Country Status (4)

Country Link
US (1) US20180346966A1 (es)
CA (1) CA3058463A1 (es)
MX (1) MX2019011503A (es)
WO (1) WO2018183865A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112011537B (zh) * 2020-09-24 2022-11-04 上海国际旅行卫生保健中心(上海海关口岸门诊部) 一组扩增李斯特氏菌mlst分型溯源的管家基因的引物组及二代测序建库方法和应用
CN113845589B (zh) * 2021-08-20 2023-04-07 扬州大学 SmcL单克隆抗体、分泌该抗体的杂交瘤细胞株及其应用和竞争ELISA检测方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6951925B1 (en) * 1992-06-11 2005-10-04 Merck Patent Gesellschaft Mit Beschrankter Processes and agents for detecting Listerias
WO2012003409A1 (en) * 2010-06-30 2012-01-05 Life Technologies Corporation Detection of listeria species in food and environmental samples, methods and compositions thereof
WO2015095047A1 (en) * 2013-12-16 2015-06-25 E. I. Du Pont De Nemours Sequences and their use for detection of genus listeria species

Also Published As

Publication number Publication date
WO2018183865A1 (en) 2018-10-04
CA3058463A1 (en) 2018-10-04
US20180346966A1 (en) 2018-12-06

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