CA2696652C - Multiplex quantitative nucleic acid amplification and melting assay - Google Patents

Multiplex quantitative nucleic acid amplification and melting assay Download PDF

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Publication number
CA2696652C
CA2696652C CA2696652A CA2696652A CA2696652C CA 2696652 C CA2696652 C CA 2696652C CA 2696652 A CA2696652 A CA 2696652A CA 2696652 A CA2696652 A CA 2696652A CA 2696652 C CA2696652 C CA 2696652C
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Prior art keywords
nucleic acid
target nucleic
melting
labeled
amplification
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CA2696652A
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English (en)
French (fr)
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CA2696652A1 (en
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Russell Higuchi
Cherie Holcomb
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2696652A 2009-03-10 2010-03-09 Multiplex quantitative nucleic acid amplification and melting assay Active CA2696652C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/400,966 US8039215B2 (en) 2009-03-10 2009-03-10 Multiplex quantitative nucleic acid amplification and melting assay
US12/400,966 2009-03-10

Publications (2)

Publication Number Publication Date
CA2696652A1 CA2696652A1 (en) 2010-09-10
CA2696652C true CA2696652C (en) 2015-09-08

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Family Applications (1)

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CA2696652A Active CA2696652C (en) 2009-03-10 2010-03-09 Multiplex quantitative nucleic acid amplification and melting assay

Country Status (6)

Country Link
US (1) US8039215B2 (https=)
EP (1) EP2228454B1 (https=)
JP (1) JP5745774B2 (https=)
CN (1) CN101831496B (https=)
CA (1) CA2696652C (https=)
ES (1) ES2589102T3 (https=)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004068110A2 (en) 2003-01-24 2004-08-12 University Of Utah Methods of predicting mortality risk by determining telomere length
US9921154B2 (en) 2011-03-18 2018-03-20 Bio-Rad Laboratories, Inc. Multiplexed digital assays
US8951939B2 (en) * 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9689028B2 (en) * 2008-12-22 2017-06-27 University Of Utah Foundation Monochrome multiplex quantitative PCR
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
JP5744038B2 (ja) * 2009-10-21 2015-07-01 コーニンクレッカ フィリップス エヌ ヴェ 増幅反応の解析ツール
AU2012231098B2 (en) * 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
JP6059453B2 (ja) * 2011-05-31 2017-01-11 アークレイ株式会社 同一又は近傍の検出波長を有する蛍光色素で修飾された複数のオリゴヌクレオチドを用いて、１種類の波長で複数の遺伝子多型を検出する方法
JP5570657B2 (ja) * 2011-10-31 2014-08-13 アークレイ株式会社 遺伝子存在量の測定方法
JP2014533506A (ja) * 2011-11-16 2014-12-15 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッドＣａｎｏｎ Ｕ．Ｓ． Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ， Ｉｎｃ． 高度に相同的なゲノム領域での突然変異の検出
WO2013113748A1 (en) * 2012-02-02 2013-08-08 Primer Design Ltd Method for detecting and genotyping target nucleic acid
EP4361609A3 (en) 2012-02-03 2024-07-17 California Institute of Technology Signal encoding and decoding in multiplexed biochemical assays
CN104662172B (zh) * 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
US9970052B2 (en) 2012-08-23 2018-05-15 Bio-Rad Laboratories, Inc. Digital assays with a generic reporter
CN102952794B (zh) * 2012-09-05 2015-02-25 张家港蓝苏生物工程有限公司 靶向基因dna分子探针的制备方法
SG10201804101XA (en) 2012-09-10 2018-07-30 Biofire Diagnostics Llc Multiple amplification cycle detection
EP2971115B1 (en) * 2013-03-13 2022-07-27 Seegene, Inc. Quantification of target nucleic acid using melting peak analysis
EP2999800B1 (en) 2013-05-22 2019-09-25 Telomere Diagnostics Inc. Measures of short telomere abundance
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
EP3034617A1 (en) 2014-12-16 2016-06-22 Marcos Isamat Riviere Method for the simultaneous identification of multiple same-genome fragments or multiple fragments from different genomes using a PCR-technique
US9944978B2 (en) 2014-12-30 2018-04-17 Telomere Diagnostics, Inc. Multiplex quantitative PCR
CA2983428C (en) * 2015-06-04 2021-12-07 Mizuho Medy Co., Ltd. Kit for together detecting multiple target nucleic acids differing from each other and detection method using the same
EP4219519A3 (en) 2016-04-01 2023-09-13 Chromacode, Inc. Competitive probes for engineering signal generation
WO2017218777A1 (en) 2016-06-17 2017-12-21 California Institute Of Technology Nucleic acid reactions and related methods and compositions
US11313000B2 (en) 2016-08-04 2022-04-26 Duke University Compositions and methods for measuring bacterial growth
US11034997B2 (en) 2016-09-15 2021-06-15 Roche Molecular Systems, Inc. Methods for performing multiplexed real-time PCR
CN106868140B (zh) * 2017-03-02 2022-10-18 北京酷搏科技有限公司 多重荧光定量pcr的方法
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
EP3697927B1 (en) 2017-10-19 2022-12-14 Bio-Rad Laboratories, Inc. Digital amplification assays with unconventional and/or inverse changes in photoluminescence
KR20200115552A (ko) * 2018-01-22 2020-10-07 루미넥스 코포레이션 별개의 용융 분석을 위한 방법 및 조성물
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
AU2019255933B2 (en) 2018-04-20 2022-08-25 Seegene, Inc. . Method and apparatus for detecting a plurality of target nucleic acid sequences in sample
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
EP4388132A1 (en) * 2021-08-19 2024-06-26 Luminex Corporation Digital amplification assay analysis method
US20250066857A1 (en) * 2021-12-27 2025-02-27 Maccura Biotechnology Co., Ltd. Method for detecting target nucleic acid
CN116356075A (zh) * 2021-12-27 2023-06-30 迈克生物股份有限公司 一种用于检测的引物探针、引物探针组及其应用
WO2024006927A1 (en) * 2022-06-29 2024-01-04 Life Technologies Corporation Compositions, kits, and methods for detecting nucleic acids using intra-channel multiplexing
CN117701689A (zh) * 2022-09-14 2024-03-15 迈克生物股份有限公司 多重pcr反应体系

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
DE3529478A1 (de) 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5405774A (en) 1986-08-22 1995-04-11 Hoffmann-La Roche Inc. DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17
US5795762A (en) 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5968799A (en) 1990-09-28 1999-10-19 Roche Molecular Systems, Inc. Purified thermostable nucleic acid polymerase enzyme from thermosipho africanus
JPH04234422A (ja) 1990-10-31 1992-08-24 Internatl Business Mach Corp <Ibm> 二重硬化エポキシバックシール処方物
DE4234086A1 (de) 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
GB9513271D0 (en) 1995-06-29 1995-09-06 Johnson James G Stress free fish harvester
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
EP1033411B1 (en) 1996-06-04 2006-02-22 University of Utah Research Foundation Fluorescent donor-acceptor pair
US6427156B1 (en) 1997-01-21 2002-07-30 Xilinx, Inc. Configurable logic block with AND gate for efficient multiplication in FPGAS
US6228628B1 (en) 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6472156B1 (en) * 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
US20040053230A1 (en) * 2001-03-07 2004-03-18 Schaffer Michael Edward Real time quantitative pcr with intercalating dye for single and multiplex target dna
US7019129B1 (en) * 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
ES2269614T3 (es) 2001-08-31 2007-04-01 The University Of Utah Research Foundation Cuantificacion de genes en tiempo real con estandares internos.
CA2516299A1 (en) * 2003-02-18 2004-09-02 Applera Corporation Compositions and methods for multiplex analysis of polynucleotides
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
US20080096199A1 (en) * 2004-10-11 2008-04-24 Reimo Tetzner Method for the Carry-Over Protection In Dna Amplification Systems Targeting Methylation Analysis Achieved by a Modified Pre-Treatment of Nucleic Acids
WO2006044994A2 (en) * 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification
US20070072211A1 (en) 2005-06-30 2007-03-29 Roche Molecular Systems, Inc. Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids
JP4815980B2 (ja) * 2005-10-05 2011-11-16 株式会社島津製作所 融解曲線測定による遺伝子増幅産物の解析方法
WO2009042728A1 (en) 2007-09-24 2009-04-02 Allelogic Biosciences Corporation Detection and /or quantification of nucleic acids
EP2116614A1 (en) 2008-05-06 2009-11-11 Qiagen GmbH Simultaneous detection of multiple nucleic acid sequences in a reaction
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay

Also Published As

Publication number Publication date
CA2696652A1 (en) 2010-09-10
EP2228454A1 (en) 2010-09-15
CN101831496B (zh) 2017-04-12
ES2589102T3 (es) 2016-11-10
JP5745774B2 (ja) 2015-07-08
EP2228454B1 (en) 2016-06-22
CN101831496A (zh) 2010-09-15
US20100233686A1 (en) 2010-09-16
JP2010207220A (ja) 2010-09-24
US8039215B2 (en) 2011-10-18

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