CA2696652C - Multiplex quantitative nucleic acid amplification and melting assay - Google Patents

Multiplex quantitative nucleic acid amplification and melting assay Download PDF

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Publication number
CA2696652C
CA2696652C CA2696652A CA2696652A CA2696652C CA 2696652 C CA2696652 C CA 2696652C CA 2696652 A CA2696652 A CA 2696652A CA 2696652 A CA2696652 A CA 2696652A CA 2696652 C CA2696652 C CA 2696652C
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Prior art keywords
nucleic acid
target nucleic
melting
labeled
amplification
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CA2696652A
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French (fr)
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CA2696652A1 (en
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Russell Higuchi
Cherie Holcomb
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2696652A 2009-03-10 2010-03-09 Multiplex quantitative nucleic acid amplification and melting assay Active CA2696652C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/400,966 US8039215B2 (en) 2009-03-10 2009-03-10 Multiplex quantitative nucleic acid amplification and melting assay
US12/400,966 2009-03-10

Publications (2)

Publication Number Publication Date
CA2696652A1 CA2696652A1 (en) 2010-09-10
CA2696652C true CA2696652C (en) 2015-09-08

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Family Applications (1)

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CA2696652A Active CA2696652C (en) 2009-03-10 2010-03-09 Multiplex quantitative nucleic acid amplification and melting assay

Country Status (6)

Country Link
US (1) US8039215B2 (https=)
EP (1) EP2228454B1 (https=)
JP (1) JP5745774B2 (https=)
CN (1) CN101831496B (https=)
CA (1) CA2696652C (https=)
ES (1) ES2589102T3 (https=)

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CN106868140B (zh) * 2017-03-02 2022-10-18 北京酷搏科技有限公司 多重荧光定量pcr的方法
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
US11072820B2 (en) 2017-10-19 2021-07-27 Bio-Rad Laboratories, Inc. Digital amplification assays with unconventional and/or inverse changes in photoluminescence
JP2021511056A (ja) * 2018-01-22 2021-05-06 ルミネックス コーポレーション 不連続融解分析のための方法および組成物
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
KR102464501B1 (ko) 2018-04-20 2022-11-17 주식회사 씨젠 샘플 내 복수의 타겟 핵산 서열의 검출을 위한 방법 및 장치
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
WO2023023533A1 (en) * 2021-08-19 2023-02-23 Luminex Corporation Digital amplification assay analysis method
WO2023125552A1 (zh) * 2021-12-27 2023-07-06 迈克生物股份有限公司 对靶标核酸进行检测的方法
CN116356075A (zh) * 2021-12-27 2023-06-30 迈克生物股份有限公司 一种用于检测的引物探针、引物探针组及其应用
JP2025522828A (ja) * 2022-06-29 2025-07-17 ライフ テクノロジーズ コーポレーション チャネル内マルチプレックス化を用いて核酸を検出するための組成物、キット及び方法
CN117701689A (zh) * 2022-09-14 2024-03-15 迈克生物股份有限公司 多重pcr反应体系

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Also Published As

Publication number Publication date
JP5745774B2 (ja) 2015-07-08
US20100233686A1 (en) 2010-09-16
CA2696652A1 (en) 2010-09-10
JP2010207220A (ja) 2010-09-24
CN101831496B (zh) 2017-04-12
CN101831496A (zh) 2010-09-15
ES2589102T3 (es) 2016-11-10
EP2228454A1 (en) 2010-09-15
US8039215B2 (en) 2011-10-18
EP2228454B1 (en) 2016-06-22

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