CN101831496B - 多重定量核酸扩增及解链测定法 - Google Patents
多重定量核酸扩增及解链测定法 Download PDFInfo
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- CN101831496B CN101831496B CN201010163977.7A CN201010163977A CN101831496B CN 101831496 B CN101831496 B CN 101831496B CN 201010163977 A CN201010163977 A CN 201010163977A CN 101831496 B CN101831496 B CN 101831496B
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- nucleic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/400,966 US8039215B2 (en) | 2009-03-10 | 2009-03-10 | Multiplex quantitative nucleic acid amplification and melting assay |
| US12/400966 | 2009-03-10 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101831496A CN101831496A (zh) | 2010-09-15 |
| CN101831496B true CN101831496B (zh) | 2017-04-12 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010163977.7A Active CN101831496B (zh) | 2009-03-10 | 2010-03-10 | 多重定量核酸扩增及解链测定法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8039215B2 (https=) |
| EP (1) | EP2228454B1 (https=) |
| JP (1) | JP5745774B2 (https=) |
| CN (1) | CN101831496B (https=) |
| CA (1) | CA2696652C (https=) |
| ES (1) | ES2589102T3 (https=) |
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| EP3351919A1 (en) | 2003-01-24 | 2018-07-25 | University of Utah | Methods of predicting mortality risk by determining telomere length |
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| US9921154B2 (en) | 2011-03-18 | 2018-03-20 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays |
| CN102439171B (zh) * | 2008-12-22 | 2017-07-14 | 犹他大学研究基金会 | 单色多重定量pcr |
| US8039215B2 (en) | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
| US8990059B2 (en) * | 2009-10-21 | 2015-03-24 | Koninklijke Philips N.V. | Analyzing tool for amplification reactions |
| CN103534360A (zh) * | 2011-03-18 | 2014-01-22 | 伯乐生命医学产品有限公司 | 借助对信号的组合使用进行的多重数字分析 |
| JP6059453B2 (ja) * | 2011-05-31 | 2017-01-11 | アークレイ株式会社 | 同一又は近傍の検出波長を有する蛍光色素で修飾された複数のオリゴヌクレオチドを用いて、1種類の波長で複数の遺伝子多型を検出する方法 |
| CN104024433B (zh) * | 2011-10-31 | 2016-11-16 | 爱科来株式会社 | 基因丰度的测定方法 |
| JP2014533506A (ja) * | 2011-11-16 | 2014-12-15 | キヤノン ユー.エス. ライフ サイエンシズ, インコーポレイテッドCanon U.S. Life Sciences, Inc. | 高度に相同的なゲノム領域での突然変異の検出 |
| WO2013113748A1 (en) * | 2012-02-02 | 2013-08-08 | Primer Design Ltd | Method for detecting and genotyping target nucleic acid |
| CN114717296B (zh) | 2012-02-03 | 2025-04-04 | 加州理工学院 | 多路生化测定中信号的编码和解码 |
| EP3901243A1 (en) * | 2012-08-03 | 2021-10-27 | California Institute of Technology | Multiplexing and quantification in pcr with reduced hardware and requirements |
| US9970052B2 (en) | 2012-08-23 | 2018-05-15 | Bio-Rad Laboratories, Inc. | Digital assays with a generic reporter |
| CN102952794B (zh) * | 2012-09-05 | 2015-02-25 | 张家港蓝苏生物工程有限公司 | 靶向基因dna分子探针的制备方法 |
| WO2014039963A1 (en) | 2012-09-10 | 2014-03-13 | Biofire Diagnostics, Inc. | Multiple amplification cycle detection |
| WO2014142575A1 (en) * | 2013-03-13 | 2014-09-18 | Seegene, Inc. | Quantification of target nucleic acid using melting peak analysis |
| CA2912216A1 (en) | 2013-05-22 | 2014-11-27 | Telomere Diagnostics, Inc. | Measures of short telomere abundance |
| WO2015147370A1 (en) * | 2014-03-28 | 2015-10-01 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures |
| EP3034617A1 (en) | 2014-12-16 | 2016-06-22 | Marcos Isamat Riviere | Method for the simultaneous identification of multiple same-genome fragments or multiple fragments from different genomes using a PCR-technique |
| WO2016108954A1 (en) | 2014-12-30 | 2016-07-07 | Telomere Diagnostics, Inc. | Multiplex quantitative pcr |
| US20180155764A1 (en) * | 2015-06-04 | 2018-06-07 | Mizuho Medy Co., Ltd. | Kit for together detecting multiple target nucleic acids differing from each other and detection method using the same |
| WO2017173035A1 (en) | 2016-04-01 | 2017-10-05 | Chromacode Inc. | Competitive probes for engineering signal generation |
| US10066263B2 (en) | 2016-06-17 | 2018-09-04 | California Institute Of Technology | Nucleic acid reactions and related methods and compositions |
| US11313000B2 (en) | 2016-08-04 | 2022-04-26 | Duke University | Compositions and methods for measuring bacterial growth |
| ES2893411T3 (es) | 2016-09-15 | 2022-02-09 | Hoffmann La Roche | Procedimientos para realizar PCR multiplexada |
| CN106868140B (zh) * | 2017-03-02 | 2022-10-18 | 北京酷搏科技有限公司 | 多重荧光定量pcr的方法 |
| US20190093155A1 (en) * | 2017-05-25 | 2019-03-28 | Roche Molecular Systems, Inc. | Multiplex Nucleic Acid Amplification Assay |
| US11072820B2 (en) | 2017-10-19 | 2021-07-27 | Bio-Rad Laboratories, Inc. | Digital amplification assays with unconventional and/or inverse changes in photoluminescence |
| JP2021511056A (ja) * | 2018-01-22 | 2021-05-06 | ルミネックス コーポレーション | 不連続融解分析のための方法および組成物 |
| US12454720B2 (en) | 2018-04-17 | 2025-10-28 | ChromaCode, Inc. | Methods and systems for multiplex analysis |
| KR102464501B1 (ko) | 2018-04-20 | 2022-11-17 | 주식회사 씨젠 | 샘플 내 복수의 타겟 핵산 서열의 검출을 위한 방법 및 장치 |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| WO2023023533A1 (en) * | 2021-08-19 | 2023-02-23 | Luminex Corporation | Digital amplification assay analysis method |
| WO2023125552A1 (zh) * | 2021-12-27 | 2023-07-06 | 迈克生物股份有限公司 | 对靶标核酸进行检测的方法 |
| CN116356075A (zh) * | 2021-12-27 | 2023-06-30 | 迈克生物股份有限公司 | 一种用于检测的引物探针、引物探针组及其应用 |
| JP2025522828A (ja) * | 2022-06-29 | 2025-07-17 | ライフ テクノロジーズ コーポレーション | チャネル内マルチプレックス化を用いて核酸を検出するための組成物、キット及び方法 |
| CN117701689A (zh) * | 2022-09-14 | 2024-03-15 | 迈克生物股份有限公司 | 多重pcr反应体系 |
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| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| DE3529478A1 (de) | 1985-08-16 | 1987-02-19 | Boehringer Mannheim Gmbh | 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung |
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2009
- 2009-03-10 US US12/400,966 patent/US8039215B2/en active Active
-
2010
- 2010-03-08 ES ES10002350.6T patent/ES2589102T3/es active Active
- 2010-03-08 EP EP10002350.6A patent/EP2228454B1/en active Active
- 2010-03-09 JP JP2010052206A patent/JP5745774B2/ja active Active
- 2010-03-09 CA CA2696652A patent/CA2696652C/en active Active
- 2010-03-10 CN CN201010163977.7A patent/CN101831496B/zh active Active
Non-Patent Citations (1)
| Title |
|---|
| Real-Time Multiplex PCR Assays;Carl T. Wittwer et al.;《METHODS》;20011231;第25卷;430-442 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP5745774B2 (ja) | 2015-07-08 |
| US20100233686A1 (en) | 2010-09-16 |
| CA2696652A1 (en) | 2010-09-10 |
| JP2010207220A (ja) | 2010-09-24 |
| CN101831496A (zh) | 2010-09-15 |
| ES2589102T3 (es) | 2016-11-10 |
| EP2228454A1 (en) | 2010-09-15 |
| US8039215B2 (en) | 2011-10-18 |
| CA2696652C (en) | 2015-09-08 |
| EP2228454B1 (en) | 2016-06-22 |
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