CN101831496B - 多重定量核酸扩增及解链测定法 - Google Patents
多重定量核酸扩增及解链测定法 Download PDFInfo
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- CN101831496B CN101831496B CN201010163977.7A CN201010163977A CN101831496B CN 101831496 B CN101831496 B CN 101831496B CN 201010163977 A CN201010163977 A CN 201010163977A CN 101831496 B CN101831496 B CN 101831496B
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- nucleic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/400966 | 2009-03-10 | ||
| US12/400,966 US8039215B2 (en) | 2009-03-10 | 2009-03-10 | Multiplex quantitative nucleic acid amplification and melting assay |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101831496A CN101831496A (zh) | 2010-09-15 |
| CN101831496B true CN101831496B (zh) | 2017-04-12 |
Family
ID=42224157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010163977.7A Active CN101831496B (zh) | 2009-03-10 | 2010-03-10 | 多重定量核酸扩增及解链测定法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8039215B2 (https=) |
| EP (1) | EP2228454B1 (https=) |
| JP (1) | JP5745774B2 (https=) |
| CN (1) | CN101831496B (https=) |
| CA (1) | CA2696652C (https=) |
| ES (1) | ES2589102T3 (https=) |
Families Citing this family (38)
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| WO2004068110A2 (en) | 2003-01-24 | 2004-08-12 | University Of Utah | Methods of predicting mortality risk by determining telomere length |
| US9921154B2 (en) | 2011-03-18 | 2018-03-20 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays |
| US8951939B2 (en) * | 2011-07-12 | 2015-02-10 | Bio-Rad Laboratories, Inc. | Digital assays with multiplexed detection of two or more targets in the same optical channel |
| US9689028B2 (en) * | 2008-12-22 | 2017-06-27 | University Of Utah Foundation | Monochrome multiplex quantitative PCR |
| US8039215B2 (en) | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
| JP5744038B2 (ja) * | 2009-10-21 | 2015-07-01 | コーニンクレッカ フィリップス エヌ ヴェ | 増幅反応の解析ツール |
| AU2012231098B2 (en) * | 2011-03-18 | 2016-09-29 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays with combinatorial use of signals |
| JP6059453B2 (ja) * | 2011-05-31 | 2017-01-11 | アークレイ株式会社 | 同一又は近傍の検出波長を有する蛍光色素で修飾された複数のオリゴヌクレオチドを用いて、1種類の波長で複数の遺伝子多型を検出する方法 |
| JP5570657B2 (ja) * | 2011-10-31 | 2014-08-13 | アークレイ株式会社 | 遺伝子存在量の測定方法 |
| JP2014533506A (ja) * | 2011-11-16 | 2014-12-15 | キヤノン ユー.エス. ライフ サイエンシズ, インコーポレイテッドCanon U.S. Life Sciences, Inc. | 高度に相同的なゲノム領域での突然変異の検出 |
| WO2013113748A1 (en) * | 2012-02-02 | 2013-08-08 | Primer Design Ltd | Method for detecting and genotyping target nucleic acid |
| EP4361609A3 (en) | 2012-02-03 | 2024-07-17 | California Institute of Technology | Signal encoding and decoding in multiplexed biochemical assays |
| CN104662172B (zh) * | 2012-08-03 | 2018-07-06 | 加州理工学院 | Pcr中具有减少的硬件和要求的多重化和定量 |
| US9970052B2 (en) | 2012-08-23 | 2018-05-15 | Bio-Rad Laboratories, Inc. | Digital assays with a generic reporter |
| CN102952794B (zh) * | 2012-09-05 | 2015-02-25 | 张家港蓝苏生物工程有限公司 | 靶向基因dna分子探针的制备方法 |
| SG10201804101XA (en) | 2012-09-10 | 2018-07-30 | Biofire Diagnostics Llc | Multiple amplification cycle detection |
| EP2971115B1 (en) * | 2013-03-13 | 2022-07-27 | Seegene, Inc. | Quantification of target nucleic acid using melting peak analysis |
| EP2999800B1 (en) | 2013-05-22 | 2019-09-25 | Telomere Diagnostics Inc. | Measures of short telomere abundance |
| WO2015147370A1 (en) | 2014-03-28 | 2015-10-01 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures |
| EP3034617A1 (en) | 2014-12-16 | 2016-06-22 | Marcos Isamat Riviere | Method for the simultaneous identification of multiple same-genome fragments or multiple fragments from different genomes using a PCR-technique |
| US9944978B2 (en) | 2014-12-30 | 2018-04-17 | Telomere Diagnostics, Inc. | Multiplex quantitative PCR |
| CA2983428C (en) * | 2015-06-04 | 2021-12-07 | Mizuho Medy Co., Ltd. | Kit for together detecting multiple target nucleic acids differing from each other and detection method using the same |
| EP4219519A3 (en) | 2016-04-01 | 2023-09-13 | Chromacode, Inc. | Competitive probes for engineering signal generation |
| WO2017218777A1 (en) | 2016-06-17 | 2017-12-21 | California Institute Of Technology | Nucleic acid reactions and related methods and compositions |
| US11313000B2 (en) | 2016-08-04 | 2022-04-26 | Duke University | Compositions and methods for measuring bacterial growth |
| US11034997B2 (en) | 2016-09-15 | 2021-06-15 | Roche Molecular Systems, Inc. | Methods for performing multiplexed real-time PCR |
| CN106868140B (zh) * | 2017-03-02 | 2022-10-18 | 北京酷搏科技有限公司 | 多重荧光定量pcr的方法 |
| US20190093155A1 (en) * | 2017-05-25 | 2019-03-28 | Roche Molecular Systems, Inc. | Multiplex Nucleic Acid Amplification Assay |
| EP3697927B1 (en) | 2017-10-19 | 2022-12-14 | Bio-Rad Laboratories, Inc. | Digital amplification assays with unconventional and/or inverse changes in photoluminescence |
| KR20200115552A (ko) * | 2018-01-22 | 2020-10-07 | 루미넥스 코포레이션 | 별개의 용융 분석을 위한 방법 및 조성물 |
| CN112424377A (zh) | 2018-04-17 | 2021-02-26 | 克罗玛科德公司 | 用于多重分析的方法和系统 |
| AU2019255933B2 (en) | 2018-04-20 | 2022-08-25 | Seegene, Inc. . | Method and apparatus for detecting a plurality of target nucleic acid sequences in sample |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| EP4388132A1 (en) * | 2021-08-19 | 2024-06-26 | Luminex Corporation | Digital amplification assay analysis method |
| US20250066857A1 (en) * | 2021-12-27 | 2025-02-27 | Maccura Biotechnology Co., Ltd. | Method for detecting target nucleic acid |
| CN116356075A (zh) * | 2021-12-27 | 2023-06-30 | 迈克生物股份有限公司 | 一种用于检测的引物探针、引物探针组及其应用 |
| WO2024006927A1 (en) * | 2022-06-29 | 2024-01-04 | Life Technologies Corporation | Compositions, kits, and methods for detecting nucleic acids using intra-channel multiplexing |
| CN117701689A (zh) * | 2022-09-14 | 2024-03-15 | 迈克生物股份有限公司 | 多重pcr反应体系 |
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| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| DE3529478A1 (de) | 1985-08-16 | 1987-02-19 | Boehringer Mannheim Gmbh | 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung |
| US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US5455170A (en) | 1986-08-22 | 1995-10-03 | Hoffmann-La Roche Inc. | Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 |
| US5405774A (en) | 1986-08-22 | 1995-04-11 | Hoffmann-La Roche Inc. | DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17 |
| US5795762A (en) | 1986-08-22 | 1998-08-18 | Roche Molecular Systems, Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5466591A (en) | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
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| JPH04234422A (ja) | 1990-10-31 | 1992-08-24 | Internatl Business Mach Corp <Ibm> | 二重硬化エポキシバックシール処方物 |
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| WO2009042728A1 (en) | 2007-09-24 | 2009-04-02 | Allelogic Biosciences Corporation | Detection and /or quantification of nucleic acids |
| EP2116614A1 (en) | 2008-05-06 | 2009-11-11 | Qiagen GmbH | Simultaneous detection of multiple nucleic acid sequences in a reaction |
| US8039215B2 (en) | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
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2009
- 2009-03-10 US US12/400,966 patent/US8039215B2/en active Active
-
2010
- 2010-03-08 ES ES10002350.6T patent/ES2589102T3/es active Active
- 2010-03-08 EP EP10002350.6A patent/EP2228454B1/en active Active
- 2010-03-09 JP JP2010052206A patent/JP5745774B2/ja active Active
- 2010-03-09 CA CA2696652A patent/CA2696652C/en active Active
- 2010-03-10 CN CN201010163977.7A patent/CN101831496B/zh active Active
Non-Patent Citations (1)
| Title |
|---|
| Real-Time Multiplex PCR Assays;Carl T. Wittwer et al.;《METHODS》;20011231;第25卷;430-442 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2696652A1 (en) | 2010-09-10 |
| EP2228454A1 (en) | 2010-09-15 |
| ES2589102T3 (es) | 2016-11-10 |
| JP5745774B2 (ja) | 2015-07-08 |
| EP2228454B1 (en) | 2016-06-22 |
| CA2696652C (en) | 2015-09-08 |
| CN101831496A (zh) | 2010-09-15 |
| US20100233686A1 (en) | 2010-09-16 |
| JP2010207220A (ja) | 2010-09-24 |
| US8039215B2 (en) | 2011-10-18 |
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| GR01 | Patent grant |