CA2511128C - Late-pcr - Google Patents

Late-pcr Download PDF

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Publication number
CA2511128C
CA2511128C CA2511128A CA2511128A CA2511128C CA 2511128 C CA2511128 C CA 2511128C CA 2511128 A CA2511128 A CA 2511128A CA 2511128 A CA2511128 A CA 2511128A CA 2511128 C CA2511128 C CA 2511128C
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CA
Canada
Prior art keywords
primer
concentration
pcr
excess
limiting
Prior art date
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Expired - Fee Related
Application number
CA2511128A
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English (en)
French (fr)
Other versions
CA2511128A1 (en
Inventor
Lawrence J. Wangh
Kenneth Pierce
Cristina Hartshorn
John Rice
J. Aquiles Sanchez
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Brandeis University
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Brandeis University
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Publication of CA2511128A1 publication Critical patent/CA2511128A1/en
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Publication of CA2511128C publication Critical patent/CA2511128C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)
  • Debugging And Monitoring (AREA)
  • Separation By Low-Temperature Treatments (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Saccharide Compounds (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Underground Structures, Protecting, Testing And Restoring Foundations (AREA)
CA2511128A 2001-12-19 2002-12-19 Late-pcr Expired - Fee Related CA2511128C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US34188601P 2001-12-19 2001-12-19
US60/341,886 2001-12-19
US10/320,893 2002-12-17
US10/320,893 US7198897B2 (en) 2001-12-19 2002-12-17 Late-PCR
PCT/US2002/040752 WO2003054233A1 (en) 2001-12-19 2002-12-19 Late-pcr

Publications (2)

Publication Number Publication Date
CA2511128A1 CA2511128A1 (en) 2003-07-03
CA2511128C true CA2511128C (en) 2011-01-18

Family

ID=26982708

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2511128A Expired - Fee Related CA2511128C (en) 2001-12-19 2002-12-19 Late-pcr

Country Status (11)

Country Link
US (3) US7198897B2 (https=)
EP (1) EP1468114B1 (https=)
JP (1) JP4355211B2 (https=)
AT (1) ATE394508T1 (https=)
AU (1) AU2002360685A1 (https=)
CA (1) CA2511128C (https=)
DE (1) DE60226500D1 (https=)
DK (1) DK1468114T3 (https=)
ES (1) ES2306807T3 (https=)
PT (1) PT1468114E (https=)
WO (1) WO2003054233A1 (https=)

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