CA2390309A1 - Procede de synthese d'un acide nucleique - Google Patents

Procede de synthese d'un acide nucleique Download PDF

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Publication number
CA2390309A1
CA2390309A1 CA002390309A CA2390309A CA2390309A1 CA 2390309 A1 CA2390309 A1 CA 2390309A1 CA 002390309 A CA002390309 A CA 002390309A CA 2390309 A CA2390309 A CA 2390309A CA 2390309 A1 CA2390309 A1 CA 2390309A1
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CA
Canada
Prior art keywords
region
complementary
oligonucleotide
synthesis
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002390309A
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English (en)
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CA2390309C (fr
Inventor
Tsugunori Notomi
Tetsu Hase
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Eiken Chemical Co Ltd
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Individual
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=14237231&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2390309(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from PCT/JP1999/006213 external-priority patent/WO2000028082A1/fr
Application filed by Individual filed Critical Individual
Publication of CA2390309A1 publication Critical patent/CA2390309A1/fr
Application granted granted Critical
Publication of CA2390309C publication Critical patent/CA2390309C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

L'invention porte sur un oligonucléotide présentant une structure nouvelle et sur un procédé de synthèse d'un acide nucléique l'utilisant comme amorce. L'oligonucléotide du côté 5' de l'amorce comporte une séquence de base sensiblement identique à la région à synthétiser lorsqu'on utilise ladite amorce comme point de départ de la synthèse. On peut ainsi synthétiser un acide nucléique sur la base d'une réaction isothermique en recourant à une simple constitution de réactif. L'invention porte également sur un procédé de synthèse d'un acide nucléique à forte spécificité sur la base du procédé ci-dessus de synthèse d'un acide nucléique.
CA2390309A 1999-11-08 2000-03-28 Procede de synthese d'un acide nucleique Expired - Lifetime CA2390309C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JPPCT/JP99/06213 1999-11-08
PCT/JP1999/006213 WO2000028082A1 (fr) 1998-11-09 1999-11-08 Procede de synthese d'acide nucleique
PCT/JP2000/001919 WO2001034790A1 (fr) 1999-11-08 2000-03-28 Procede de synthese d'un acide nucleique

Publications (2)

Publication Number Publication Date
CA2390309A1 true CA2390309A1 (fr) 2001-05-17
CA2390309C CA2390309C (fr) 2012-09-25

Family

ID=14237231

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2390309A Expired - Lifetime CA2390309C (fr) 1999-11-08 2000-03-28 Procede de synthese d'un acide nucleique

Country Status (9)

Country Link
KR (1) KR100612551B1 (fr)
CN (2) CN100393875C (fr)
BR (1) BR0015382B1 (fr)
CA (1) CA2390309C (fr)
IL (1) IL149446A0 (fr)
NO (1) NO331732B1 (fr)
RU (1) RU2252964C2 (fr)
WO (1) WO2001034790A1 (fr)
ZA (1) ZA200203293B (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7713691B2 (en) 1998-06-24 2010-05-11 Enzo Life Sciences, Inc. Modified nucleic acid polymers and methods for their production
US10745745B2 (en) 2013-03-15 2020-08-18 Labrador Diagnostics Llc Nucleic acid amplification
US11254960B2 (en) 2013-03-15 2022-02-22 Labrador Diagnostics Llc Nucleic acid amplification

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8445664B2 (en) 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
AU2001244677A1 (en) * 2000-04-07 2001-10-23 Eiken Kagaku Kabushiki Kaisha Method of amplifying nucleic acid by using double-stranded nucleic acid as template
DE60139331D1 (de) 2000-09-19 2009-09-03 Eiken Chemical Verfahren zur polynukleotidsynthese
KR100806680B1 (ko) * 2003-11-21 2008-02-26 한국표준과학연구원 Ｐｃｒ용 열순환 반응기의 성능시험용 다중 ｐｃｒ 조성물
JP5818018B2 (ja) * 2009-03-26 2015-11-18 厦門艾徳生物医薬科技有限公司 核酸増幅を用いる環状プライマー及びその応用
CN101671674B (zh) * 2009-03-27 2010-09-22 郑立谋 一种用于核酸扩增的环形引物及其应用
TWI600766B (zh) 2012-08-09 2017-10-01 財團法人工業技術研究院 用於偵測一目標核苷酸序列中之一特定區域的一突變及／或多形性的套組
CA3094792A1 (fr) 2013-03-13 2014-09-18 Illumina, Inc. Procedes et compositions pour le sequencage d'acide nucleique
WO2015075198A1 (fr) * 2013-11-22 2015-05-28 Orion Diagnostica Oy Détection d'acides nucléiques par amplification basée sur l'invasion de brin
US10364458B2 (en) 2014-07-16 2019-07-30 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
BR112017007912A2 (pt) * 2014-10-17 2018-01-23 Illumina Cambridge Limited transposon de preservação de contiguidade
US11559801B2 (en) 2014-11-03 2023-01-24 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
PT3234188T (pt) 2014-12-15 2020-01-16 Cepheid Amplificação de ácidos nucleicos em base exponencial superior a 2
WO2018132939A1 (fr) * 2017-01-17 2018-07-26 中国科学院过程工程研究所 Procédé de synthèse d'acide nucléique à température constante
EP3808843A1 (fr) * 2017-09-14 2021-04-21 Zhongke Xinray (Suzhou) Biological Science Technologies Co., Ltd. Procédé et kit de synthèse d'acide nucléique dans des conditions de température constantes
WO2020092134A1 (fr) 2018-10-29 2020-05-07 Cepheid Amplification d'acides nucléiques exponentielle de base 3 avec temps d'amplification réduit à l'aide d'amorces chevauchantes imbriquées
WO2020148610A1 (fr) 2019-01-15 2020-07-23 3M Innovative Properties Company Amorces d'amplification isotherme à médiation par boucle pour la détection d'e. coli produisant la toxine de shiga (stec)
WO2021165828A1 (fr) 2020-02-17 2021-08-26 3M Innovative Properties Company Amorces d'amplification isothermique à médiation par boucle pour la détection de vibrio parahaemolyticus et leurs utilisations
CA3186580A1 (fr) 2020-06-12 2021-12-16 Sherlock Biosciences, Inc. Detection du sars-cov-2 basee sur crispr
WO2022029449A1 (fr) 2020-08-07 2022-02-10 Oxford Nanopore Technologies Limited Procédés d'identification de codes-barres d'acide nucléique
CN113201583B (zh) * 2021-04-29 2022-02-08 国科宁波生命与健康产业研究院 恒温条件下合成核酸的方法及试剂盒和应用
EP4105327A4 (fr) * 2021-04-29 2023-01-18 Ningbo Institute of Life and Health Industry University of Chinese Academy of Sciences Procédé de synthèse d'acide nucléique dans des conditions de température constante, kit et application

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4213029A1 (de) * 1991-09-26 1993-04-01 Boehringer Mannheim Gmbh Verfahren zur spezifischen vervielfaeltigung von nukleins aeuresequenzen
ATE161586T1 (de) * 1991-10-11 1998-01-15 Behringwerke Ag Verfahren zur herstellung eines polynukleotids zum gebrauch in ''single-primer'' amplifizierung und phosphorothioat-enthaltende oligonukleotide als primer in nukleinsäureamplifizierung
AU3729393A (en) * 1992-02-20 1993-09-13 State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University, The Boomerand DNA amplification
FR2721945B1 (fr) * 1994-07-04 1996-10-18 David Fabrice Accroissement genique, un procede d'amplicication genique isotherme et ses applications
FR2726277B1 (fr) * 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
EP1231281B1 (fr) * 1999-11-08 2006-02-01 Eiken Kagaku Kabushiki Kaisha Methode de detection d'une variation ou d'un polymorphisme

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7713691B2 (en) 1998-06-24 2010-05-11 Enzo Life Sciences, Inc. Modified nucleic acid polymers and methods for their production
US8133989B2 (en) 1998-06-24 2012-03-13 Enzo Diagnostics, Inc. Nucleic acid primer/construct compositions
US8288092B2 (en) 1998-06-24 2012-10-16 Enzo Life Sciences, Inc. Modified nucleic acid polymers and methods for their production
US10745745B2 (en) 2013-03-15 2020-08-18 Labrador Diagnostics Llc Nucleic acid amplification
US11254960B2 (en) 2013-03-15 2022-02-22 Labrador Diagnostics Llc Nucleic acid amplification
US11603558B2 (en) 2013-03-15 2023-03-14 Labrador Diagnostics Llc Nucleic acid amplification
US11649487B2 (en) 2013-03-15 2023-05-16 Labrador Diagnostics Llc Nucleic acid amplification

Also Published As

Publication number Publication date
RU2252964C2 (ru) 2005-05-27
IL149446A0 (en) 2002-11-10
CN100393875C (zh) 2008-06-11
CN1876843B (zh) 2012-09-05
WO2001034790A1 (fr) 2001-05-17
KR100612551B1 (ko) 2006-08-11
BR0015382B1 (pt) 2014-04-29
CN1876843A (zh) 2006-12-13
ZA200203293B (en) 2003-03-26
NO20022171D0 (no) 2002-05-07
RU2002115268A (ru) 2004-01-27
CN1420928A (zh) 2003-05-28
NO20022171L (no) 2002-07-04
NO331732B1 (no) 2012-03-12
KR20020064896A (ko) 2002-08-10
BR0015382A (pt) 2002-07-02
CA2390309C (fr) 2012-09-25

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