CA2390309C - Procede de synthese d'un acide nucleique - Google Patents

Procede de synthese d'un acide nucleique Download PDF

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Publication number
CA2390309C
CA2390309C CA2390309A CA2390309A CA2390309C CA 2390309 C CA2390309 C CA 2390309C CA 2390309 A CA2390309 A CA 2390309A CA 2390309 A CA2390309 A CA 2390309A CA 2390309 C CA2390309 C CA 2390309C
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Canada
Prior art keywords
region
nucleic acid
complementary
nucleotide sequence
chain
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Expired - Lifetime
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CA2390309A
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CA2390309A1 (fr
Inventor
Tsugunori Notomi
Tetsu Hase
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Eiken Chemical Co Ltd
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Eiken Chemical Co Ltd
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=14237231&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2390309(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from PCT/JP1999/006213 external-priority patent/WO2000028082A1/fr
Application filed by Eiken Chemical Co Ltd filed Critical Eiken Chemical Co Ltd
Publication of CA2390309A1 publication Critical patent/CA2390309A1/fr
Application granted granted Critical
Publication of CA2390309C publication Critical patent/CA2390309C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

L'invention porte sur un oligonucléotide présentant une structure nouvelle et sur un procédé de synthèse d'un acide nucléique l'utilisant comme amorce. L'oligonucléotide du côté 5' de l'amorce comporte une séquence de base sensiblement identique à la région à synthétiser lorsqu'on utilise ladite amorce comme point de départ de la synthèse. On peut ainsi synthétiser un acide nucléique sur la base d'une réaction isothermique en recourant à une simple constitution de réactif. L'invention porte également sur un procédé de synthèse d'un acide nucléique à forte spécificité sur la base du procédé ci-dessus de synthèse d'un acide nucléique.
CA2390309A 1999-11-08 2000-03-28 Procede de synthese d'un acide nucleique Expired - Lifetime CA2390309C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/JP1999/006213 WO2000028082A1 (fr) 1998-11-09 1999-11-08 Procede de synthese d'acide nucleique
JPPCT/JP99/06213 1999-11-08
PCT/JP2000/001919 WO2001034790A1 (fr) 1999-11-08 2000-03-28 Procede de synthese d'un acide nucleique

Publications (2)

Publication Number Publication Date
CA2390309A1 CA2390309A1 (fr) 2001-05-17
CA2390309C true CA2390309C (fr) 2012-09-25

Family

ID=14237231

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2390309A Expired - Lifetime CA2390309C (fr) 1999-11-08 2000-03-28 Procede de synthese d'un acide nucleique

Country Status (9)

Country Link
KR (1) KR100612551B1 (fr)
CN (2) CN100393875C (fr)
BR (1) BR0015382B1 (fr)
CA (1) CA2390309C (fr)
IL (1) IL149446A0 (fr)
NO (1) NO331732B1 (fr)
RU (1) RU2252964C2 (fr)
WO (1) WO2001034790A1 (fr)
ZA (1) ZA200203293B (fr)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8445664B2 (en) 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
EP1275715B1 (fr) * 2000-04-07 2015-07-15 Eiken Kagaku Kabushiki Kaisha Procede d'amplification et d'detection d'acide nucleique par utilisation d'acide nucleique a double brin en tant que modele
ATE437225T1 (de) 2000-09-19 2009-08-15 Eiken Chemical Verfahren zur polynukleotidsynthese
KR100806680B1 (ko) * 2003-11-21 2008-02-26 한국표준과학연구원 Ｐｃｒ용 열순환 반응기의 성능시험용 다중 ｐｃｒ 조성물
EP2412718B1 (fr) * 2009-03-26 2016-10-12 Xiamen Amoy Diagnostics Co., Ltd Amorce en forme de boucle employée en amplification d'acides nucléiques et son utilisation
CN101671674B (zh) * 2009-03-27 2010-09-22 郑立谋 一种用于核酸扩增的环形引物及其应用
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
TWI600766B (zh) 2012-08-09 2017-10-01 財團法人工業技術研究院 用於偵測一目標核苷酸序列中之一特定區域的一突變及／或多形性的套組
DK3553175T3 (da) 2013-03-13 2021-08-23 Illumina Inc Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek
US20140302504A1 (en) 2013-03-15 2014-10-09 Theranos, Inc. Nucleic Acid Amplification
EP2971123B1 (fr) 2013-03-15 2021-05-05 Labrador Diagnostics LLC Amplification d'acide nucléique
CA2930786C (fr) * 2013-11-22 2022-05-10 Orion Diagnostica Oy Detection d'acides nucleiques par amplification basee sur l'invasion de brin
CN106661600B (zh) 2014-07-16 2021-04-06 唐恩生物科技股份有限公司 用于扩增核酸样品的等温方法
KR102643955B1 (ko) * 2014-10-17 2024-03-07 일루미나 케임브리지 리미티드 근접 보존 전위
CN107208012B (zh) 2014-11-03 2021-06-29 唐恩生物科学公司 用于细胞、孢子或病毒捕获和破碎的装置和方法
CA2971006C (fr) 2014-12-15 2024-05-21 Cepheid Amplification d'acides nucleiques superieure a 2 sur une base exponentielle
WO2018132939A1 (fr) * 2017-01-17 2018-07-26 中国科学院过程工程研究所 Procédé de synthèse d'acide nucléique à température constante
EP3476938B1 (fr) * 2017-09-14 2020-05-13 Zhongke Xinray (Suzhou) Biological Science Technologies Co., Ltd. Procédé et kit de synthèse d'acide nucléique dans des conditions de température constantes
EP3874064A1 (fr) 2018-10-29 2021-09-08 Cepheid Amplification d'acides nucléiques exponentielle de base 3 avec temps d'amplification réduit à l'aide d'amorces chevauchantes imbriquées
US20230183819A1 (en) 2019-01-15 2023-06-15 Neogen Food Safety Us Holdco Corporation Loop-mediated isothermal amplification primers for shiga toxin-producing e. coli (stec) detection
JP2023515397A (ja) 2020-02-17 2023-04-13 スリーエム イノベイティブ プロパティズ カンパニー ビブリオ・パラヘモリティカス検出のためのループ介在等温増幅プライマー及びその使用
EP4165218A1 (fr) 2020-06-12 2023-04-19 Sherlock Biosciences, Inc. Détection du sars-cov-2 basée sur crispr
US20220059187A1 (en) 2020-08-07 2022-02-24 Oxford Nanopore Technologies Limited Methods of detecting nucleic acid barcodes
CN115109840A (zh) * 2021-03-18 2022-09-27 厦门大学 一种分析目标核酸中突变的存在及其类型的方法
EP4105327A4 (fr) * 2021-04-29 2023-01-18 Ningbo Institute of Life and Health Industry University of Chinese Academy of Sciences Procédé de synthèse d'acide nucléique dans des conditions de température constante, kit et application
CN113201583B (zh) * 2021-04-29 2022-02-08 国科宁波生命与健康产业研究院 恒温条件下合成核酸的方法及试剂盒和应用
KR20240072313A (ko) 2022-11-10 2024-05-24 (주)레보스케치 황색포도상구균 감염 진단을 위한 rt-lamp용 조성물 및 이의 용도

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4213029A1 (de) * 1991-09-26 1993-04-01 Boehringer Mannheim Gmbh Verfahren zur spezifischen vervielfaeltigung von nukleins aeuresequenzen
ATE161586T1 (de) * 1991-10-11 1998-01-15 Behringwerke Ag Verfahren zur herstellung eines polynukleotids zum gebrauch in ''single-primer'' amplifizierung und phosphorothioat-enthaltende oligonukleotide als primer in nukleinsäureamplifizierung
AU3729393A (en) * 1992-02-20 1993-09-13 State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University, The Boomerand DNA amplification
FR2721945B1 (fr) * 1994-07-04 1996-10-18 David Fabrice Accroissement genique, un procede d'amplicication genique isotherme et ses applications
FR2726277B1 (fr) * 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
EP1231281B1 (fr) * 1999-11-08 2006-02-01 Eiken Kagaku Kabushiki Kaisha Methode de detection d'une variation ou d'un polymorphisme

Also Published As

Publication number Publication date
IL149446A0 (en) 2002-11-10
CA2390309A1 (fr) 2001-05-17
CN1876843B (zh) 2012-09-05
RU2002115268A (ru) 2004-01-27
BR0015382B1 (pt) 2014-04-29
KR100612551B1 (ko) 2006-08-11
NO331732B1 (no) 2012-03-12
WO2001034790A1 (fr) 2001-05-17
CN1876843A (zh) 2006-12-13
RU2252964C2 (ru) 2005-05-27
CN100393875C (zh) 2008-06-11
NO20022171L (no) 2002-07-04
BR0015382A (pt) 2002-07-02
CN1420928A (zh) 2003-05-28
KR20020064896A (ko) 2002-08-10
ZA200203293B (en) 2003-03-26
NO20022171D0 (no) 2002-05-07

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