CA2390309C - Procede de synthese d'un acide nucleique - Google Patents

Procede de synthese d'un acide nucleique Download PDF

Info

Publication number
CA2390309C
CA2390309C CA2390309A CA2390309A CA2390309C CA 2390309 C CA2390309 C CA 2390309C CA 2390309 A CA2390309 A CA 2390309A CA 2390309 A CA2390309 A CA 2390309A CA 2390309 C CA2390309 C CA 2390309C
Authority
CA
Canada
Prior art keywords
region
nucleic acid
complementary
nucleotide sequence
chain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2390309A
Other languages
English (en)
Other versions
CA2390309A1 (fr
Inventor
Tsugunori Notomi
Tetsu Hase
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Eiken Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=14237231&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2390309(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from PCT/JP1999/006213 external-priority patent/WO2000028082A1/fr
Application filed by Eiken Chemical Co Ltd filed Critical Eiken Chemical Co Ltd
Publication of CA2390309A1 publication Critical patent/CA2390309A1/fr
Application granted granted Critical
Publication of CA2390309C publication Critical patent/CA2390309C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

L'invention porte sur un oligonucléotide présentant une structure nouvelle et sur un procédé de synthèse d'un acide nucléique l'utilisant comme amorce. L'oligonucléotide du côté 5' de l'amorce comporte une séquence de base sensiblement identique à la région à synthétiser lorsqu'on utilise ladite amorce comme point de départ de la synthèse. On peut ainsi synthétiser un acide nucléique sur la base d'une réaction isothermique en recourant à une simple constitution de réactif. L'invention porte également sur un procédé de synthèse d'un acide nucléique à forte spécificité sur la base du procédé ci-dessus de synthèse d'un acide nucléique.
CA2390309A 1999-11-08 2000-03-28 Procede de synthese d'un acide nucleique Expired - Lifetime CA2390309C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/JP1999/006213 WO2000028082A1 (fr) 1998-11-09 1999-11-08 Procede de synthese d'acide nucleique
JPPCT/JP99/06213 1999-11-08
PCT/JP2000/001919 WO2001034790A1 (fr) 1999-11-08 2000-03-28 Procede de synthese d'un acide nucleique

Publications (2)

Publication Number Publication Date
CA2390309A1 CA2390309A1 (fr) 2001-05-17
CA2390309C true CA2390309C (fr) 2012-09-25

Family

ID=14237231

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2390309A Expired - Lifetime CA2390309C (fr) 1999-11-08 2000-03-28 Procede de synthese d'un acide nucleique

Country Status (9)

Country Link
KR (1) KR100612551B1 (fr)
CN (2) CN100393875C (fr)
BR (1) BR0015382B1 (fr)
CA (1) CA2390309C (fr)
IL (1) IL149446A0 (fr)
NO (1) NO331732B1 (fr)
RU (1) RU2252964C2 (fr)
WO (1) WO2001034790A1 (fr)
ZA (1) ZA200203293B (fr)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US8445664B2 (en) 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
CN100422323C (zh) * 2000-04-07 2008-10-01 荣研化学株式会社 使用双链核酸为模板扩增核酸的方法
ATE437225T1 (de) 2000-09-19 2009-08-15 Eiken Chemical Verfahren zur polynukleotidsynthese
KR100806680B1 (ko) * 2003-11-21 2008-02-26 한국표준과학연구원 Ｐｃｒ용 열순환 반응기의 성능시험용 다중 ｐｃｒ 조성물
PL2412718T3 (pl) 2009-03-26 2017-06-30 Xiamen Amoy Diagnostics Co., Ltd. Primer w kształcie pętli stosowany w amplifikacji kwasów nukleinowych i jego zastosowanie
CN101671674B (zh) * 2009-03-27 2010-09-22 郑立谋 一种用于核酸扩增的环形引物及其应用
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
TWI600766B (zh) * 2012-08-09 2017-10-01 財團法人工業技術研究院 用於偵測一目標核苷酸序列中之一特定區域的一突變及／或多形性的套組
DK3553175T3 (da) 2013-03-13 2021-08-23 Illumina Inc Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek
AU2014233150A1 (en) 2013-03-15 2015-09-17 Theranos Ip Company, Llc Nucleic acid amplification
CA2903874C (fr) 2013-03-15 2024-02-20 Theranos, Inc. Amplification d'acides nucleiques
KR102324117B1 (ko) * 2013-11-22 2021-11-10 오리온 디아그노스티카 오와이 가닥 침입에 기초한 증폭에 의한 핵산 검출
EP3169793B1 (fr) 2014-07-16 2022-07-06 Tangen Biosciences Inc. Procédés isothermes pour l'amplification d'échantillons d'acide nucléique
AU2015331739B2 (en) 2014-10-17 2021-12-02 Illumina Cambridge Limited Contiguity preserving transposition
EP3215260B1 (fr) 2014-11-03 2020-01-15 Tangen Biosciences Inc. Appareil et procédé pour la capture et la rupture de cellules, de spores ou de virus
JP6804462B2 (ja) 2014-12-15 2020-12-23 セファイド 指数関数の底が２より大きい核酸増幅
WO2018132939A1 (fr) * 2017-01-17 2018-07-26 中国科学院过程工程研究所 Procédé de synthèse d'acide nucléique à température constante
EP3476938B1 (fr) * 2017-09-14 2020-05-13 Zhongke Xinray (Suzhou) Biological Science Technologies Co., Ltd. Procédé et kit de synthèse d'acide nucléique dans des conditions de température constantes
CN113227398B (zh) 2018-10-29 2025-03-14 塞弗德公司 在减少的扩增时间下使用巢式重叠引物的指数基数-3核酸扩增
CN113302314A (zh) 2019-01-15 2021-08-24 3M创新有限公司 用于产志贺毒素大肠杆菌(stec)检测的环介导的等温扩增引物
CN115103919A (zh) 2020-02-17 2022-09-23 3M创新有限公司 用于副溶血弧菌检测的环介导的等温扩增引物及其用途
AU2021287968A1 (en) 2020-06-12 2023-02-09 Sherlock Biosciences, Inc. CRISPR-based SARS-CoV-2 detection
EP4193363A1 (fr) 2020-08-07 2023-06-14 Oxford Nanopore Technologies plc Procédés d'identification de codes-barres d'acide nucléique
CN115109840B (zh) * 2021-03-18 2025-08-12 厦门大学 一种分析目标核酸中突变的存在及其类型的方法
WO2022226870A1 (fr) * 2021-04-29 2022-11-03 中国科学院大学宁波生命与健康产业研究院 Procédé de synthèse d'acide nucléique dans des conditions de température constante, kit et application
CN113201583B (zh) * 2021-04-29 2022-02-08 国科宁波生命与健康产业研究院 恒温条件下合成核酸的方法及试剂盒和应用
KR102908488B1 (ko) 2022-11-10 2026-01-07 (주)레보스케치 황색포도상구균 감염 진단을 위한 rt-lamp용 조성물 및 이의 용도

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR0148265B1 (ko) * 1988-12-16 1998-10-15 에프.지이.엠 헤르만스 자가-지속 서열 복제 시스템
DE4213029A1 (de) * 1991-09-26 1993-04-01 Boehringer Mannheim Gmbh Verfahren zur spezifischen vervielfaeltigung von nukleins aeuresequenzen
DK0549107T3 (da) * 1991-10-11 1998-08-31 Dade Behring Marburg Gmbh Fremgangsmåde til fremstilling af et polynukleotidtil anvendelse ved enkeltprimeramplifikation og phosphorthioatholdige oli
AU3729393A (en) * 1992-02-20 1993-09-13 State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University, The Boomerand DNA amplification
FR2721945B1 (fr) * 1994-07-04 1996-10-18 David Fabrice Accroissement genique, un procede d'amplicication genique isotherme et ses applications
FR2726277B1 (fr) * 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
AU1057801A (en) * 1999-11-08 2001-06-06 Eiken Kagaku Kabushiki Kaisha Method of detecting variation or polymorphism

Also Published As

Publication number Publication date
CN1876843A (zh) 2006-12-13
ZA200203293B (en) 2003-03-26
WO2001034790A1 (fr) 2001-05-17
BR0015382A (pt) 2002-07-02
CN100393875C (zh) 2008-06-11
KR100612551B1 (ko) 2006-08-11
NO20022171D0 (no) 2002-05-07
NO20022171L (no) 2002-07-04
NO331732B1 (no) 2012-03-12
BR0015382B1 (pt) 2014-04-29
RU2002115268A (ru) 2004-01-27
CN1876843B (zh) 2012-09-05
IL149446A0 (en) 2002-11-10
RU2252964C2 (ru) 2005-05-27
KR20020064896A (ko) 2002-08-10
CA2390309A1 (fr) 2001-05-17
CN1420928A (zh) 2003-05-28

Similar Documents

Publication Publication Date Title
CA2390309C (fr) Procede de synthese d'un acide nucleique
US20040132144A1 (en) Method of synthesizing nucleic acid
JPWO2000028082A1 (ja) 核酸の合成方法
JP3897805B2 (ja) 核酸の増幅法およびこれを利用した変異核酸の検出法
JP2002330796A (ja) 核酸の合成方法
JP2002345499A (ja) 核酸配列を検出する方法及びその方法に使用するキット
AU779160B2 (en) Method of synthesizing nucleic acids
JP3942627B2 (ja) 変異核酸の検出法
JPWO2002090538A1 (ja) 核酸を合成する方法
TWI221855B (en) Process for synthesizing nucleic acid
HK1110902B (en) Process for synthesizing nucleic acid
JP2001309785A (ja) 遺伝子配列間の相違検出方法
HK1154635B (en) Process for synthesizing nucleic acid
HK1029378B (en) Process for synthesizing nucleic acid

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20200328