CA2202167C - Molecular tagging system - Google Patents

Molecular tagging system Download PDF

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Publication number
CA2202167C
CA2202167C CA002202167A CA2202167A CA2202167C CA 2202167 C CA2202167 C CA 2202167C CA 002202167 A CA002202167 A CA 002202167A CA 2202167 A CA2202167 A CA 2202167A CA 2202167 C CA2202167 C CA 2202167C
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Canada
Prior art keywords
polynucleotides
oligonucleotide
tag
nucleotides
microparticles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002202167A
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English (en)
French (fr)
Other versions
CA2202167A1 (en
Inventor
Sydney Brenner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lynx Therapeutics Inc
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Lynx Therapeutics Inc
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Filing date
Publication date
Application filed by Lynx Therapeutics Inc filed Critical Lynx Therapeutics Inc
Priority to CA002441634A priority Critical patent/CA2441634A1/en
Publication of CA2202167A1 publication Critical patent/CA2202167A1/en
Application granted granted Critical
Publication of CA2202167C publication Critical patent/CA2202167C/en
Anticipated expiration legal-status Critical
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12N15/09—Recombinant DNA-technology
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B80/00—Linkers or spacers specially adapted for combinatorial chemistry or libraries, e.g. traceless linkers or safety-catch linkers
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    • B01J2219/00497—Features relating to the solid phase supports
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00718—Type of compounds synthesised
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  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Control Of Driving Devices And Active Controlling Of Vehicle (AREA)
  • Magnetic Heads (AREA)
  • Pyrane Compounds (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Saccharide Compounds (AREA)
  • Air Transport Of Granular Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
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CA002202167A 1994-10-13 1995-10-12 Molecular tagging system Expired - Fee Related CA2202167C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002441634A CA2441634A1 (en) 1994-10-13 1995-10-12 Molecular tagging system

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US32234894A 1994-10-13 1994-10-13
US08/322,348 1994-10-13
US08/358,810 US5604097A (en) 1994-10-13 1994-12-19 Methods for sorting polynucleotides using oligonucleotide tags
US08/358,810 1994-12-19
PCT/US1995/012791 WO1996012014A1 (en) 1994-10-13 1995-10-12 Molecular tagging system

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002441634A Division CA2441634A1 (en) 1994-10-13 1995-10-12 Molecular tagging system

Publications (2)

Publication Number Publication Date
CA2202167A1 CA2202167A1 (en) 1996-04-25
CA2202167C true CA2202167C (en) 2003-12-16

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CA002202167A Expired - Fee Related CA2202167C (en) 1994-10-13 1995-10-12 Molecular tagging system

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US (4) US5604097A (https=)
EP (3) EP2110445A3 (https=)
JP (6) JP4206130B2 (https=)
KR (2) KR970707279A (https=)
AT (1) ATE323159T1 (https=)
AU (2) AU712929B2 (https=)
CA (1) CA2202167C (https=)
CZ (1) CZ86697A3 (https=)
DE (1) DE69534930T2 (https=)
FI (1) FI971473A7 (https=)
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USRE39793E1 (en) 2007-08-21
FI971473L (fi) 1997-06-04
JP4480380B2 (ja) 2010-06-16
JP2006320321A (ja) 2006-11-30
NO971644D0 (no) 1997-04-10
HUT77916A (hu) 1998-10-28
DE69534930D1 (de) 2006-05-24
JP4712822B2 (ja) 2011-06-29
US5635400A (en) 1997-06-03
AU4277896A (en) 1996-05-06
US5604097A (en) 1997-02-18
JP2005065704A (ja) 2005-03-17
EP0793718B1 (en) 2006-04-12
JPH10507357A (ja) 1998-07-21
JP4480544B2 (ja) 2010-06-16
FI971473A7 (fi) 1997-06-04
EP2110445A2 (en) 2009-10-21
AU5266399A (en) 1999-12-09
AU712929B2 (en) 1999-11-18
US5654413A (en) 1997-08-05
KR19990022543A (ko) 1999-03-25
DE69534930T2 (de) 2007-01-11
EP2110445A3 (en) 2010-06-02
EP0793718A1 (en) 1997-09-10
CZ86697A3 (en) 1997-09-17
EP1724348A2 (en) 2006-11-22
EP1724348A3 (en) 2008-10-08
JP2004089198A (ja) 2004-03-25
JP4206130B2 (ja) 2009-01-07
FI971473A0 (fi) 1997-04-09
NO971644L (no) 1997-06-02
JP2008301806A (ja) 2008-12-18
ATE323159T1 (de) 2006-04-15
AU784741B2 (en) 2006-06-08
CA2202167A1 (en) 1996-04-25

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