CA1316133C - Method of using bar1 for secreting foreign proteins - Google Patents
Method of using bar1 for secreting foreign proteinsInfo
- Publication number
- CA1316133C CA1316133C CA000521206A CA521206A CA1316133C CA 1316133 C CA1316133 C CA 1316133C CA 000521206 A CA000521206 A CA 000521206A CA 521206 A CA521206 A CA 521206A CA 1316133 C CA1316133 C CA 1316133C
- Authority
- CA
- Canada
- Prior art keywords
- promoter
- fragment
- gene
- cell
- barl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US79130585A | 1985-10-25 | 1985-10-25 | |
US791,305 | 1985-10-25 | ||
USPCT/US86/02198 | 1986-10-20 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA1316133C true CA1316133C (en) | 1993-04-13 |
Family
ID=25153298
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA000521206A Expired - Fee Related CA1316133C (en) | 1985-10-25 | 1986-10-23 | Method of using bar1 for secreting foreign proteins |
Country Status (13)
Country | Link |
---|---|
EP (1) | EP0243465A1 (cs) |
JP (1) | JP2523562B2 (cs) |
CN (1) | CN1027179C (cs) |
AU (2) | AU6543286A (cs) |
CA (1) | CA1316133C (cs) |
CZ (1) | CZ284251B6 (cs) |
DK (1) | DK320287A (cs) |
FI (1) | FI872801A (cs) |
HU (1) | HU206897B (cs) |
IE (1) | IE63822B1 (cs) |
SK (1) | SK279041B6 (cs) |
UA (1) | UA41863C2 (cs) |
WO (1) | WO1987002670A1 (cs) |
Families Citing this family (54)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK58285D0 (da) * | 1984-05-30 | 1985-02-08 | Novo Industri As | Peptider samt fremstilling og anvendelse deraf |
JP2730923B2 (ja) * | 1987-10-02 | 1998-03-25 | ザイモジェネティクス,インコーポレイティド | Bar1分泌シグナル |
US5206699A (en) * | 1988-05-06 | 1993-04-27 | Gersan Establishment | Sensing a narrow frequency band of radiation and gemstones |
AU633020B2 (en) * | 1988-07-23 | 1993-01-21 | Novozymes Delta Limited | New secretory leader sequences |
AU7493491A (en) * | 1990-03-13 | 1991-10-10 | Hawaii Biotechnology Group, Inc. | (neurospora) expression system |
DK82893D0 (da) * | 1993-07-08 | 1993-07-08 | Novo Nordisk As | Peptid |
CA2214116C (en) | 1995-03-17 | 2011-05-24 | Novo Nordisk A/S | Novel endoglucanases |
CZ297338B6 (cs) | 1996-03-01 | 2006-11-15 | Novo Nordisk A/S | Peptid potlacující chut k jídlu, farmaceutický prostredek jej obsahující a jeho pouzití |
EP1250154B1 (en) | 2000-01-10 | 2009-07-01 | Maxygen Holdings Ltd | G-csf conjugates |
EP1982732A3 (en) | 2000-02-11 | 2011-06-08 | Bayer HealthCare LLC | Factor VII or VIIA-like conjugates |
BR0207576A (pt) | 2001-02-27 | 2004-04-27 | Maxygen Aps | Variante glicosilada de um polipeptìdeo de interferon beta precursor (ifnb), processos de aumentar a glicosilação in vivo de uma molécula de ifnb precursora, de produzir uma molécula de ifnb glicosilada, para preparar uma variante conjugada e de tratar um mamìfero com esclerose múltiplam composição farmacêutica, molécula de ifnb variante, sequência de nucleotìdeo, vetor de expressão, célula hospedeira de glicosilação conjugado, e, uso de um conjugado |
ES2432967T3 (es) | 2001-03-22 | 2013-12-05 | Novo Nordisk Health Care Ag | Derivados del Factor VII de coagulación |
US6534059B2 (en) | 2001-06-05 | 2003-03-18 | Advanced Biotherapy, Inc. | Compositions and methods for treating hyperimmune response in the eye |
AU2002333211B2 (en) | 2001-09-27 | 2008-05-08 | Novo Nordisk Health Care Ag | Human coagulation factor VII polypeptides |
ES2381110T3 (es) | 2003-09-09 | 2012-05-23 | Novo Nordisk Health Care Ag | Polipéptidos de factor VII de coagulación |
ES2357550T3 (es) | 2005-04-18 | 2011-04-27 | Novo Nordisk A/S | Variantes de la il-21. |
ES2368500T3 (es) | 2005-08-16 | 2011-11-17 | Novo Nordisk A/S | Método para producir polipéptidos de insulina maduros. |
US20090055942A1 (en) | 2005-09-14 | 2009-02-26 | Novo Nordisk Healthcare A/G | Human Coagulation Factor VII Polypeptides |
EP2407561A3 (en) | 2006-03-06 | 2012-05-30 | Humagene, Inc. | A method for the preparation of recombinant human fibrinogen |
WO2008030558A2 (en) | 2006-09-08 | 2008-03-13 | Ambrx, Inc. | Modified human plasma polypeptide or fc scaffolds and their uses |
ES2464282T3 (es) | 2006-09-27 | 2014-06-02 | Novo Nordisk A/S | Método para preparar polipéptidos de insulina madura |
US8114630B2 (en) | 2007-05-02 | 2012-02-14 | Ambrx, Inc. | Modified interferon beta polypeptides and their uses |
NZ603812A (en) | 2007-11-20 | 2014-06-27 | Ambrx Inc | Modified insulin polypeptides and their uses |
UA103774C2 (uk) | 2008-07-23 | 2013-11-25 | Амбркс, Інк. | Модифіковані поліпептиди бичачого гранулоцитарного колонієстимулювального фактора (g-csf) та їх застосування |
US8349325B2 (en) | 2008-12-23 | 2013-01-08 | Abbott Laboratories | Soluble FMS-like tyrosine kinase-1 (sFLT-1) antibody and related composition, kit, methods of using, and materials and method for making |
EP2406282A1 (en) | 2009-03-11 | 2012-01-18 | Novo Nordisk A/S | Interleukin-21 variants having antagonistic binding to the il-21 receptor |
EP3395828A1 (en) | 2009-07-17 | 2018-10-31 | Rigshospitalet | Inhibitors of complement activation |
CN102666570B (zh) | 2009-12-01 | 2015-12-02 | 诺沃—诺迪斯克有限公司 | 新的肽基α-羟基甘氨酸α-酰胺化裂合酶 |
AU2010341518B2 (en) | 2009-12-21 | 2014-01-09 | Ambrx, Inc. | Modified porcine somatotropin polypeptides and their uses |
NZ600361A (en) | 2009-12-21 | 2014-06-27 | Ambrx Inc | Modified bovine somatotropin polypeptides and their uses |
EP3815708A1 (en) | 2010-03-05 | 2021-05-05 | Omeros Corporation | Chimeric inhibitor molecules of complement activation |
US20110229921A1 (en) | 2010-03-18 | 2011-09-22 | Abbott Laboratories | METHODS OF ASSAYING URINARY NEUTROPHIL GELATINASE-ASSOCIATED LIPOCALIN (uNGAL) IN THE PROGNOSIS OF CADAVERIC KIDNEY TRANSPLANT FUNCTION IN A PATIENT, INCLUDING A PATIENT DIAGNOSED WITH DELAYED GRAFT FUNCTION (DGF), A METHOD OF ASSAYING uNGAL IN THE ASSESSMENT OF RISK OF DGF IN A PATIENT DIAGNOSED WITH EARLY GRAFT FUNCTION (EGF), AND RELATED KITS |
WO2011163558A1 (en) | 2010-06-25 | 2011-12-29 | Abbott Laboratories | Materials and methods for assay of anti-hepatitis c virus (hcv) antibodies |
MA34521B1 (fr) | 2010-08-17 | 2013-09-02 | Ambrx Inc | Polypeptides de relaxine modifiés et leurs utilisations |
AR083006A1 (es) | 2010-09-23 | 2013-01-23 | Lilly Co Eli | Formulaciones para el factor estimulante de colonias de granulocitos (g-csf) bovino y variantes de las mismas |
US20120115244A1 (en) | 2010-11-09 | 2012-05-10 | Abbott Laboratories | Materials and methods for immunoassay of pterins |
ES2627117T3 (es) | 2010-12-09 | 2017-07-26 | Institut Pasteur | Método basado en MGMT para obtener un rendimiento elevado de expresión de proteínas recombinantes |
KR102007061B1 (ko) | 2011-12-09 | 2019-08-02 | 앵스띠뛰 파스퇴르 | 다중 면역 스크리닝 분석 |
US8993248B2 (en) | 2011-12-31 | 2015-03-31 | Abbott Laboratories | Truncated human vitamin D binding protein and mutation and fusion thereof and related materials and methods of use |
ES2676029T3 (es) | 2012-02-09 | 2018-07-16 | Var2 Pharmaceuticals Aps | Direccionamiento de glicanos de sulfato de condroitina |
CN104704118A (zh) | 2012-10-15 | 2015-06-10 | 诺和诺德保健Ag(股份有限公司) | 凝血因子vii多肽 |
JP2014128262A (ja) * | 2012-11-27 | 2014-07-10 | Kirin Brewery Co Ltd | 接合能を持つ酵母細胞株のスクリーニング方法 |
MX2020011529A (es) | 2018-05-01 | 2021-01-29 | Ambrx Inc | Un metodo para optimizar la expresion de anticuerpos. |
US20210278406A1 (en) | 2018-07-13 | 2021-09-09 | Varct Diagnostic Aps | Isolation of circulating cells of fetal origin using recombinant malaria protein var2csa |
BR112021004488A2 (pt) | 2018-09-11 | 2021-06-01 | Ambrx, Inc. | conjugados de polipeptídeo de interleucina-2 e seus usos |
JP2022512746A (ja) | 2018-10-19 | 2022-02-07 | アンブルックス,インコーポレイテッド | インターロイキン-10ポリペプチド複合体、その二量体、およびそれらの使用 |
MX2021009644A (es) | 2019-02-12 | 2021-09-08 | Ambrx Inc | Composiciones que contienen, metodos y usos de conjugados de anticuerpo-agonista de tlr. |
CA3174114A1 (en) | 2020-03-11 | 2021-09-16 | Ambrx, Inc. | Interleukin-2 polypeptide conjugates and methods of use thereof |
CA3175523A1 (en) | 2020-04-13 | 2021-10-21 | Antti Virtanen | Methods, complexes and kits for detecting or determining an amount of a .beta.-coronavirus antibody in a sample |
US20220043000A1 (en) | 2020-08-04 | 2022-02-10 | Abbott Laboratories | Methods and kits for detecting sars-cov-2 protein in a sample |
KR20230073200A (ko) | 2020-08-20 | 2023-05-25 | 암브룩스, 인코포레이티드 | 항체-tlr 작용제 접합체, 그 방법 및 용도 |
EP4271998A1 (en) | 2020-12-30 | 2023-11-08 | Abbott Laboratories | Methods for determining sars-cov-2 antigen and anti-sars-cov-2 antibody in a sample |
IL307282A (en) | 2021-04-03 | 2023-11-01 | Ambrx Inc | Antidote conjugates against HER2 and their applications |
CN115806889B (zh) * | 2022-09-28 | 2024-06-11 | 山东大学 | 一种提高基因表达水平的酿酒酵母工程菌及其构建方法与应用 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4546082A (en) * | 1982-06-17 | 1985-10-08 | Regents Of The Univ. Of California | E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins |
US4613572A (en) * | 1983-08-15 | 1986-09-23 | Kansas State University Research Foundation | Yeast BAR1 gene plasmid |
JPS61247384A (ja) * | 1984-10-18 | 1986-11-04 | チモ−ジエネテイツクス インコ−ポレ−テツド | 酵母中でプラスミノ−ゲン活性化因子を発現する方法 |
DE3537176C2 (de) * | 1984-10-18 | 1994-06-09 | Zymogenetics Inc | Gewebeplasminogenaktivator und Verfahren zu seiner Herstellung |
-
1986
- 1986-10-20 EP EP86906638A patent/EP0243465A1/en not_active Withdrawn
- 1986-10-20 UA UA4203156A patent/UA41863C2/uk unknown
- 1986-10-20 AU AU65432/86A patent/AU6543286A/en not_active Abandoned
- 1986-10-20 JP JP61505713A patent/JP2523562B2/ja not_active Expired - Fee Related
- 1986-10-20 WO PCT/US1986/002198 patent/WO1987002670A1/en active Application Filing
- 1986-10-20 HU HU865248A patent/HU206897B/hu not_active IP Right Cessation
- 1986-10-23 CA CA000521206A patent/CA1316133C/en not_active Expired - Fee Related
- 1986-10-23 IE IE280486A patent/IE63822B1/en not_active IP Right Cessation
- 1986-10-25 CN CN86107554A patent/CN1027179C/zh not_active Expired - Lifetime
- 1986-10-27 SK SK7764-86A patent/SK279041B6/sk unknown
- 1986-10-27 CZ CS867764A patent/CZ284251B6/cs not_active IP Right Cessation
-
1987
- 1987-06-23 DK DK320287A patent/DK320287A/da not_active Application Discontinuation
- 1987-06-24 FI FI872801A patent/FI872801A/fi not_active Application Discontinuation
-
1991
- 1991-04-02 AU AU74003/91A patent/AU676132B2/en not_active Ceased
Also Published As
Publication number | Publication date |
---|---|
AU6543286A (en) | 1987-05-19 |
DK320287D0 (da) | 1987-06-23 |
UA41863C2 (uk) | 2001-10-15 |
SK776486A3 (en) | 1998-06-03 |
FI872801A0 (fi) | 1987-06-24 |
JP2523562B2 (ja) | 1996-08-14 |
CZ776486A3 (en) | 1996-09-11 |
FI872801A (fi) | 1987-06-24 |
SK279041B6 (sk) | 1998-06-03 |
CZ284251B6 (cs) | 1998-10-14 |
EP0243465A1 (en) | 1987-11-04 |
DK320287A (da) | 1987-06-23 |
AU676132B2 (en) | 1997-03-06 |
JPS63501614A (ja) | 1988-06-23 |
AU7400391A (en) | 1991-07-18 |
HU206897B (en) | 1993-01-28 |
IE63822B1 (en) | 1995-06-14 |
CN1027179C (zh) | 1994-12-28 |
HUT43624A (en) | 1987-11-30 |
IE862804L (en) | 1987-04-25 |
WO1987002670A1 (en) | 1987-05-07 |
CN86107554A (zh) | 1987-08-26 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
MKLA | Lapsed |