BRPI9813021B1 - processo para a preparação microbiana de aminoácidos de l-lisina, l-treonina, l-homosserina e glutamato, vetor, e célula transformada - Google Patents
processo para a preparação microbiana de aminoácidos de l-lisina, l-treonina, l-homosserina e glutamato, vetor, e célula transformadaInfo
- Publication number
- BRPI9813021B1 BRPI9813021B1 BRPI9813021A BR9813021A BRPI9813021B1 BR PI9813021 B1 BRPI9813021 B1 BR PI9813021B1 BR PI9813021 A BRPI9813021 A BR PI9813021A BR 9813021 A BR9813021 A BR 9813021A BR PI9813021 B1 BRPI9813021 B1 BR PI9813021B1
- Authority
- BR
- Brazil
- Prior art keywords
- gene
- pyruvate carboxylase
- amino acids
- vector
- glutamate
- Prior art date
Links
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/14—Glutamic acid; Glutamine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/20—Aspartic acid; Asparagine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
- C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
- C12Y604/01001—Pyruvate carboxylase (6.4.1.1)
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (7)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19743894 | 1997-10-04 | ||
DE19831609A DE19831609B4 (de) | 1997-10-04 | 1998-07-14 | Verfahren zur Herstellung von Aminosäuren der Aspartat- und/oder Glutamatfamilie und im Verfahren einsetzbare Mittel |
PCT/EP1998/006210 WO1999018228A2 (de) | 1997-10-04 | 1998-09-30 | Verfahren zur mikrobiellen herstellung von aminosäuren der aspartat- und/oder glutamatfamilie und im verfahren einsetzbare mittel |
Publications (2)
Publication Number | Publication Date |
---|---|
BR9813021A BR9813021A (pt) | 2000-08-15 |
BRPI9813021B1 true BRPI9813021B1 (pt) | 2016-04-05 |
Family
ID=26040582
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI9813021A BRPI9813021B1 (pt) | 1997-10-04 | 1998-09-30 | processo para a preparação microbiana de aminoácidos de l-lisina, l-treonina, l-homosserina e glutamato, vetor, e célula transformada |
Country Status (15)
Country | Link |
---|---|
US (2) | US7267967B1 (pt) |
EP (1) | EP1015621B1 (pt) |
JP (1) | JP2002508921A (pt) |
CN (1) | CN1323171C (pt) |
AT (1) | ATE290602T1 (pt) |
AU (1) | AU741038B2 (pt) |
BR (1) | BRPI9813021B1 (pt) |
CA (1) | CA2305577A1 (pt) |
DE (1) | DE59812638D1 (pt) |
ES (1) | ES2238773T3 (pt) |
HU (1) | HU224055B1 (pt) |
ID (1) | ID26644A (pt) |
RU (1) | RU2231552C2 (pt) |
SK (1) | SK284235B6 (pt) |
WO (1) | WO1999018228A2 (pt) |
Families Citing this family (85)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2194122B1 (en) | 1998-04-13 | 2015-12-02 | The University Of Georgia Research Foundation, Inc. | Pyruvate carboxylase overexpression for enhanced production of oxaloacetate-derived biochemicals in microbial cells |
DE19931317A1 (de) * | 1999-07-07 | 2001-01-11 | Degussa | L-Lysin produzierende coryneforme Bakterien und Verfahren zur Herstellung von L-Lysin |
AU2203399A (en) * | 1998-12-23 | 2000-07-31 | Massachusetts Institute Of Technology | Pyruvate carboxylase from (corynebacterium glutamicum) |
US6171833B1 (en) | 1998-12-23 | 2001-01-09 | Massachusetts Institute Of Technology | Pyruvate carboxylase from corynebacterium glutamicum |
JP2000201692A (ja) | 1999-01-13 | 2000-07-25 | Ajinomoto Co Inc | 発酵法によるl―グルタミン酸の製造法 |
TW200734459A (en) | 1999-10-04 | 2007-09-16 | Ajinomoto Kk | Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria |
DE19947792A1 (de) * | 1999-10-05 | 2001-04-12 | Degussa | Neue für das Irp Gen codierende Nukleotidsequenzen |
US7132272B2 (en) | 1999-10-05 | 2006-11-07 | Degussa Ag | Nucleotide sequence encoding corynebacterium glutamicum leucine response regulatory protein |
RU2207376C2 (ru) * | 1999-10-14 | 2003-06-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" | Способ получения l-аминокислоты методом ферментации, штамм бактерии escherichia coli - продуцент l-аминокислоты (варианты) |
US6949374B2 (en) | 2000-05-04 | 2005-09-27 | Degussa Ag | FadD15 gene of Corynebacterium glutamicum, encoding an acyl-CoA synthase polypeptide |
DE10021831A1 (de) * | 2000-05-04 | 2001-11-08 | Degussa | Neue für das fadD15-Gen kodierende Nukleotidsequenzen |
DE10032350A1 (de) | 2000-07-04 | 2002-01-24 | Degussa | Neue für das mdhA-Gen kodierende Nukleotidsequenzen |
EP1307562B1 (en) * | 2000-08-10 | 2004-12-15 | Degussa AG | Nucleotide sequences which code for the lysr3 gene |
DE10039049A1 (de) | 2000-08-10 | 2002-02-21 | Degussa | Neue für das IysR3-Gen kodierende Nukleotidsequenzen |
DE10042051A1 (de) * | 2000-08-26 | 2002-03-07 | Degussa | Neue für das cstA-Gen kodierende Nukleotidsequenzen |
PL364087A1 (en) * | 2001-02-21 | 2004-12-13 | Basf Aktiengesellschaft | Method for the production of d-pantothenic acid and/or salts thereof as adjunct for animal feedstuffs |
JP2004523237A (ja) * | 2001-02-21 | 2004-08-05 | ビーエーエスエフ アクチェンゲゼルシャフト | 動物飼料添加剤としてのd−パントテン酸および/またはその塩の製造方法 |
DE10108223A1 (de) * | 2001-02-21 | 2002-10-10 | Basf Ag | Verfahren zur Herstellung von D-Pantothensäure und/oder deren Salze als Zusatz zu Tierfuttermitteln |
DE10108225A1 (de) * | 2001-02-21 | 2002-10-10 | Basf Ag | Verfahren zur Herstellung von D-Pantothensäure und/oder deren Salze als Zusatz zu Tierfuttermitteln |
EP1407035B1 (en) | 2001-07-18 | 2006-04-12 | Degussa AG | Process for the preparation of l-amino acids using strains of the enterobacteriaceae family which contain an attenuated aspa gene |
ATE434663T1 (de) | 2001-07-18 | 2009-07-15 | Evonik Degussa Gmbh | Verfahren zur herstellung von l-threonin durch enterobakteriaceae-stämmen mit verstärkter exprimierung der succ und sucd gene |
CA2456416A1 (en) * | 2001-08-06 | 2003-02-20 | Degussa Ag | Coryneform bacteria which produce chemical compounds ii |
US8278076B2 (en) | 2002-02-20 | 2012-10-02 | University Of Georgia Research Foundation, Inc. | Microbial production of pyruvate and pyruvate derivatives |
DE10303571A1 (de) | 2003-01-30 | 2004-08-12 | Degussa Ag | Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae |
DE10316109A1 (de) | 2003-04-09 | 2004-10-21 | Degussa Ag | Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae |
DE102004005836A1 (de) | 2004-02-06 | 2005-09-15 | Degussa Ag | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae |
DE102004037572A1 (de) * | 2004-08-03 | 2006-02-23 | Degussa Ag | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae |
DE102005018835A1 (de) | 2005-04-22 | 2006-11-02 | Degussa Ag | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung verbesserter Stämme der Familie Enterobacteriaceae |
DE102005043979A1 (de) | 2005-09-15 | 2007-03-22 | Forschungszentrum Jülich GmbH | Verfahren zur Produktion von Aminosäuren in aminosäureproduzierenden Mikroorganismen |
JP2009118740A (ja) | 2006-03-03 | 2009-06-04 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
DE102006032634A1 (de) | 2006-07-13 | 2008-01-17 | Evonik Degussa Gmbh | Verfahren zur Herstellung von L-Aminosäuren |
EP2066782A4 (en) * | 2006-09-15 | 2010-02-03 | Cj Cheiljedang Corp | CORYNEBACTERIA WITH IMPROVED L-LYSINE PRODUCTIVITY AND METHOD FOR THE PREPARATION OF L-LYSINE THEREWITH |
JP2010041920A (ja) | 2006-12-19 | 2010-02-25 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
KR100830826B1 (ko) | 2007-01-24 | 2008-05-19 | 씨제이제일제당 (주) | 코리네박테리아를 이용하여 글리세롤을 포함한탄소원으로부터 발효산물을 생산하는 방법 |
JP2010088301A (ja) | 2007-02-01 | 2010-04-22 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
JP2010110216A (ja) | 2007-02-20 | 2010-05-20 | Ajinomoto Co Inc | L−アミノ酸または核酸の製造方法 |
DE102007051024A1 (de) | 2007-03-05 | 2008-09-11 | Evonik Degussa Gmbh | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae |
EP1975241A1 (de) | 2007-03-29 | 2008-10-01 | Evonik Degussa GmbH | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
JP2010226956A (ja) * | 2007-07-23 | 2010-10-14 | Ajinomoto Co Inc | L−リジンの製造法 |
EP2192170B1 (en) | 2007-09-04 | 2017-02-15 | Ajinomoto Co., Inc. | Amino acid-producing microorganism and method of producing amino acid |
DE102007044134A1 (de) | 2007-09-15 | 2009-03-19 | Evonik Degussa Gmbh | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
DE102007052270A1 (de) | 2007-11-02 | 2009-05-07 | Evonik Degussa Gmbh | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
EP2060636A1 (de) | 2007-11-14 | 2009-05-20 | Evonik Degussa GmbH | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
JP2011067095A (ja) | 2008-01-10 | 2011-04-07 | Ajinomoto Co Inc | 発酵法による目的物質の製造法 |
CN102348806A (zh) | 2008-01-23 | 2012-02-08 | 味之素株式会社 | L-氨基酸的生产方法 |
EP2098597A1 (de) | 2008-03-04 | 2009-09-09 | Evonik Degussa GmbH | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
US8932861B2 (en) | 2008-04-10 | 2015-01-13 | Cj Cheiljedang Corporation | Transformation vector comprising transposon, microorganisms transformed with the vector, and method for producing L-lysine using the microorganism |
KR101126041B1 (ko) | 2008-04-10 | 2012-03-19 | 씨제이제일제당 (주) | 트랜스포존을 이용한 형질전환용 벡터, 상기 벡터로형질전환된 미생물 및 이를 이용한 l-라이신 생산방법 |
DE102008001874A1 (de) | 2008-05-20 | 2009-11-26 | Evonik Degussa Gmbh | Verfahren zur Herstellung von L-Aminosäuren |
DE102008002309A1 (de) | 2008-06-09 | 2009-12-10 | Evonik Degussa Gmbh | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
DE102008044768A1 (de) | 2008-08-28 | 2010-03-04 | Evonik Degussa Gmbh | Verfahren zur Herstellung von organisch-chemischen Verbindungen unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
CN102177246B (zh) | 2008-09-08 | 2015-02-11 | 味之素株式会社 | 生产l-氨基酸的微生物和l-氨基酸的生产方法 |
JP2012029565A (ja) | 2008-11-27 | 2012-02-16 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
EP2267145A1 (de) | 2009-06-24 | 2010-12-29 | Evonik Degussa GmbH | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
WO2011013707A1 (ja) | 2009-07-29 | 2011-02-03 | 味の素株式会社 | L-アミノ酸の製造法 |
JP2012223092A (ja) | 2009-08-28 | 2012-11-15 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
JP2013013329A (ja) | 2009-11-06 | 2013-01-24 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
MX2012009816A (es) | 2010-02-25 | 2012-12-10 | Council Scient Ind Res | Procesos para la produccion de arginina utilizando corynebacterium glutamicum atcc 21831 o corynebacterium glutamicum atcc 21493 en un medio de fermentacion que comprende bagazo de mandioca o semilla del fruto de panapen como una fuente de carbono. |
KR101145943B1 (ko) | 2010-03-11 | 2012-05-15 | 씨제이제일제당 (주) | L-아미노산 생산능을 갖는 미생물 및 이를 이용하여 l-아미노산을 생산하는 방법 |
JP5796282B2 (ja) * | 2010-08-31 | 2015-10-21 | 東レ株式会社 | コリネ型細菌による化学品の製造方法 |
RU2496867C2 (ru) * | 2011-04-25 | 2013-10-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | Способ получения l-аминокислоты семейства глутамата с использованием коринеформной бактерии |
EP2540834A1 (en) | 2011-06-29 | 2013-01-02 | Metabolic Explorer | Method for the preparation of 1,3-propanediol |
CN105483145B (zh) * | 2011-12-21 | 2021-04-06 | Cj第一制糖株式会社 | 利用具有产生l-赖氨酸能力的微生物产生l-赖氨酸的方法 |
JP5831669B2 (ja) | 2013-05-13 | 2015-12-09 | 味の素株式会社 | L−アミノ酸の製造法 |
EA201591846A1 (ru) * | 2013-05-17 | 2016-08-31 | Ксилеко, Инк. | Обработка биомассы |
JP2016165225A (ja) | 2013-07-09 | 2016-09-15 | 味の素株式会社 | 有用物質の製造方法 |
JP2016192903A (ja) | 2013-09-17 | 2016-11-17 | 味の素株式会社 | 海藻由来バイオマスからのl−アミノ酸の製造方法 |
ES2761596T3 (es) | 2013-10-02 | 2020-05-20 | Ajinomoto Kk | Aparato de control de amoniaco y método de control de amoniaco |
JP6459962B2 (ja) | 2013-10-21 | 2019-01-30 | 味の素株式会社 | L−アミノ酸の製造法 |
JP6519476B2 (ja) | 2013-10-23 | 2019-05-29 | 味の素株式会社 | 目的物質の製造法 |
CN105505969A (zh) * | 2014-09-26 | 2016-04-20 | 中国科学院天津工业生物技术研究所 | 一种提高l-苏氨酸转化率的方法及其应用 |
JP6623690B2 (ja) | 2015-10-30 | 2019-12-25 | 味の素株式会社 | グルタミン酸系l−アミノ酸の製造法 |
JP7066977B2 (ja) | 2017-04-03 | 2022-05-16 | 味の素株式会社 | L-アミノ酸の製造法 |
DE102017004751A1 (de) | 2017-05-18 | 2018-11-22 | Forschungszentrum Jülich GmbH | Pyruvatcarboxylase und für die Pyruvatcarboxylase kodierende DNA, Plasmid enthaltend die DNA, sowie Mikroorganismus zur Produktion und verfahren zur Herstellung von Produkten, deren Bioynthese Oxalacetat als Vorstufe beeinhaltet und Chromosom |
EP3467099A1 (en) | 2017-10-05 | 2019-04-10 | Evonik Degussa GmbH | Method for the fermentative production of l-amino acids |
EP3608409A1 (en) | 2018-08-09 | 2020-02-12 | Evonik Operations GmbH | Process for preparing l amino acids using improved strains of the enterobacteriaceae family |
EP3847270A1 (en) | 2018-09-07 | 2021-07-14 | Archer Daniels Midland Company | Engineered strains of corynebacteria |
RU2019128538A (ru) | 2018-09-26 | 2021-03-11 | Эвоник Оперейшенс ГмбХ | Способ ферментативного получения l-лизина |
WO2020071538A1 (en) | 2018-10-05 | 2020-04-09 | Ajinomoto Co., Inc. | Method for producing target substance by bacterial fermentation |
CN113755492B (zh) * | 2020-07-20 | 2023-05-30 | 中国科学院天津工业生物技术研究所 | 丙酮酸羧化酶基因启动子的突变体及其应用 |
JPWO2022092018A1 (pt) | 2020-10-28 | 2022-05-05 | ||
WO2023222510A1 (en) | 2022-05-18 | 2023-11-23 | Evonik Operations Gmbh | Biotechnological production of desferrioxamines and analogs thereof |
WO2023222515A1 (en) | 2022-05-18 | 2023-11-23 | Evonik Operations Gmbh | Biotechnological production of bisucaberins, desferrioxamines and analogs thereof |
WO2023222505A1 (en) | 2022-05-18 | 2023-11-23 | Evonik Operations Gmbh | Biotechnological production of monomers of bisucaberins, desferrioxamines and analogs thereof |
US20240117393A1 (en) | 2022-09-30 | 2024-04-11 | Ajinomoto Co., Inc. | Method for producing l-amino acid |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2223754B (en) | 1988-09-12 | 1992-07-22 | Degussa | Dna encoding phosphoenolpyruvate carboxylase |
DE4201085A1 (de) | 1992-01-17 | 1993-07-22 | Degussa | Verfahren zur erhoehung der leistungsfaehigkeit l-lysin ausscheidender coryneformer bakterien |
HU219600B (hu) * | 1993-08-24 | 2001-05-28 | Ajinomoto Co., Inc. | Mutáns foszfo-enolpiruvát-karboxiláz, ennek génje, valamint eljárás aminosav előállítására |
US5939307A (en) * | 1996-07-30 | 1999-08-17 | The Archer-Daniels-Midland Company | Strains of Escherichia coli, methods of preparing the same and use thereof in fermentation processes for l-threonine production |
DE60030400T2 (de) * | 1999-07-09 | 2007-09-13 | Degussa Gmbh | Für den opac-gen kodierende nukleotidsequenzen |
DE19941478A1 (de) * | 1999-09-01 | 2001-03-08 | Degussa | Neue für das thrE-Gen codierende Nukleotidsequenzen und Verfahren zur fermentativen Herstellung von L-Threonin mit coryneformen Bakterien |
DE19959329A1 (de) * | 1999-12-09 | 2001-06-13 | Degussa | Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien |
DE10154102A1 (de) * | 2001-11-02 | 2003-05-15 | Degussa | Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae |
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1998
- 1998-09-30 SK SK4812000A patent/SK284235B6/sk unknown
- 1998-09-30 CN CNB988098385A patent/CN1323171C/zh not_active Expired - Lifetime
- 1998-09-30 BR BRPI9813021A patent/BRPI9813021B1/pt active IP Right Grant
- 1998-09-30 JP JP2000515022A patent/JP2002508921A/ja active Pending
- 1998-09-30 ID IDW20000634A patent/ID26644A/id unknown
- 1998-09-30 US US09/529,043 patent/US7267967B1/en not_active Expired - Fee Related
- 1998-09-30 RU RU2000111541/13A patent/RU2231552C2/ru not_active IP Right Cessation
- 1998-09-30 DE DE59812638T patent/DE59812638D1/de not_active Expired - Lifetime
- 1998-09-30 AT AT98954301T patent/ATE290602T1/de not_active IP Right Cessation
- 1998-09-30 WO PCT/EP1998/006210 patent/WO1999018228A2/de active IP Right Grant
- 1998-09-30 EP EP98954301A patent/EP1015621B1/de not_active Expired - Lifetime
- 1998-09-30 HU HU0004381A patent/HU224055B1/hu not_active IP Right Cessation
- 1998-09-30 CA CA002305577A patent/CA2305577A1/en not_active Abandoned
- 1998-09-30 AU AU11482/99A patent/AU741038B2/en not_active Ceased
- 1998-09-30 ES ES98954301T patent/ES2238773T3/es not_active Expired - Lifetime
-
2007
- 2007-04-05 US US11/732,869 patent/US20070202571A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
SK284235B6 (en) | 2004-11-03 |
ES2238773T3 (es) | 2005-09-01 |
CN1275167A (zh) | 2000-11-29 |
CN1323171C (zh) | 2007-06-27 |
DE59812638D1 (de) | 2005-04-14 |
ID26644A (id) | 2001-01-25 |
EP1015621A2 (de) | 2000-07-05 |
US20070202571A1 (en) | 2007-08-30 |
AU1148299A (en) | 1999-04-27 |
EP1015621B1 (de) | 2005-03-09 |
HUP0004381A1 (en) | 2001-03-28 |
HU224055B1 (hu) | 2005-05-30 |
RU2231552C2 (ru) | 2004-06-27 |
CA2305577A1 (en) | 1999-04-15 |
HUP0004381A3 (en) | 2003-08-28 |
AU741038B2 (en) | 2001-11-22 |
WO1999018228A2 (de) | 1999-04-15 |
ATE290602T1 (de) | 2005-03-15 |
BR9813021A (pt) | 2000-08-15 |
SK4812000A3 (en) | 2000-09-12 |
WO1999018228A3 (de) | 1999-05-20 |
JP2002508921A (ja) | 2002-03-26 |
US7267967B1 (en) | 2007-09-11 |
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