BRPI0710949A2 - Método para indução de diferenciação de células tronco pluripotentes em cardiomiócitos - Google Patents

Método para indução de diferenciação de células tronco pluripotentes em cardiomiócitos Download PDF

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Publication number
BRPI0710949A2
BRPI0710949A2 BRPI0710949-0A BRPI0710949A BRPI0710949A2 BR PI0710949 A2 BRPI0710949 A2 BR PI0710949A2 BR PI0710949 A BRPI0710949 A BR PI0710949A BR PI0710949 A2 BRPI0710949 A2 BR PI0710949A2
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Prior art keywords
pluripotent stem
stem cells
period
cardiomyocytes
canonical wnt
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BRPI0710949-0A
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English (en)
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Koshimizu Uichi
Tanaka Tomofumi
Kawashima Kayoko
Kadokura Michinori
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Asubio Pharma Co., Ltd.
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Publication of BRPI0710949A2 publication Critical patent/BRPI0710949A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Cardiology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

MÉTODO PARA INDUÇçO DE DIFERENCIAÇçO DE CÉLULAS TRONCO PLURIPOTENTES EM CARDIOMIàCITOS. A presente invenção fornece um método para a indução de diferenciação em cardiomiácitos, de maneira eficiente e seletiva, a partir de células tronco. Um método para indução de diferenciação em cardiomiácitos a partir de células tronco pluripotentes, que compreende: (i) o cultivo das células tronco pluripotentes em um meio de cultura não contendo qualquer substância que promova a ativação da via de sinalização Wnt canônica durante o período de tempo entre o início da indução de diferenciação e 24 horas antes do período de elevada expressão de gene Wnt canônico; e, então, (ii) o cultivo das células tronco pluripotentes em um meio de cultura contendo uma substância, que promova a ativação da via de sinalização Wnt canônica durante um período de tempo de 24 a 96 horas, partindo de 24 a O horas antes do período de elevada expressão de gene Wnt canônico.
BRPI0710949-0A 2006-04-28 2007-04-27 Método para indução de diferenciação de células tronco pluripotentes em cardiomiócitos BRPI0710949A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2006125148 2006-04-28
JP2006-125148 2006-04-28
JP2007-019531 2007-01-30
JP2007019531 2007-01-30
PCT/JP2007/059242 WO2007126077A1 (ja) 2006-04-28 2007-04-27 多能性幹細胞から心筋細胞を分化誘導する方法

Publications (1)

Publication Number Publication Date
BRPI0710949A2 true BRPI0710949A2 (pt) 2012-03-06

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BRPI0710949-0A BRPI0710949A2 (pt) 2006-04-28 2007-04-27 Método para indução de diferenciação de células tronco pluripotentes em cardiomiócitos

Country Status (11)

Country Link
US (1) US8293529B2 (pt)
EP (2) EP2014766A4 (pt)
JP (1) JP5149791B2 (pt)
KR (2) KR101234544B1 (pt)
CN (1) CN101426902B (pt)
AU (1) AU2007244226B2 (pt)
BR (1) BRPI0710949A2 (pt)
CA (1) CA2650685C (pt)
IL (1) IL194970A (pt)
RU (1) RU2433174C2 (pt)
WO (1) WO2007126077A1 (pt)

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Also Published As

Publication number Publication date
CA2650685C (en) 2014-02-04
EP2014766A4 (en) 2009-12-02
CA2650685A1 (en) 2007-11-08
US20090325288A1 (en) 2009-12-31
US8293529B2 (en) 2012-10-23
EP2014766A1 (en) 2009-01-14
EP2457994A1 (en) 2012-05-30
IL194970A (en) 2012-05-31
JPWO2007126077A1 (ja) 2009-09-10
RU2433174C2 (ru) 2011-11-10
AU2007244226B2 (en) 2012-09-20
KR20090009267A (ko) 2009-01-22
RU2008146991A (ru) 2010-06-10
KR101234544B1 (ko) 2013-03-07
KR20120139856A (ko) 2012-12-27
KR101346047B1 (ko) 2013-12-31
AU2007244226A1 (en) 2007-11-08
IL194970A0 (en) 2011-08-01
JP5149791B2 (ja) 2013-02-20
CN101426902A (zh) 2009-05-06
CN101426902B (zh) 2013-03-27
WO2007126077A1 (ja) 2007-11-08

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