SG10201808863UA - Combined chemical and genetic approaches for generation of induced pluripotent stem cells - Google Patents

Combined chemical and genetic approaches for generation of induced pluripotent stem cells

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Publication number
SG10201808863UA
SG10201808863UA SG10201808863UA SG10201808863UA SG10201808863UA SG 10201808863U A SG10201808863U A SG 10201808863UA SG 10201808863U A SG10201808863U A SG 10201808863UA SG 10201808863U A SG10201808863U A SG 10201808863UA SG 10201808863U A SG10201808863U A SG 10201808863UA
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Singapore
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generation
stem cells
pluripotent stem
induced pluripotent
genetic approaches
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SG10201808863UA
Inventor
Yan Shi
Caroline Desponts
Sheng Ding
Hongyan Zhou
Tongxiang Lin
Wenlin Li
Saiyong Zhu
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Scripps Research Inst
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Publication of SG10201808863UA publication Critical patent/SG10201808863UA/en

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Abstract

COMBINED CHEMICAL AND GENETIC APPROACHES FOR GENERATION OF INDUCED PLURIPOTENT STEM CELLS The present invention provides for identification and use of small molecules to induce pluripotency in mammalian cells as well as other methods of inducing pluripotency. [Figure ] 82
SG10201808863UA 2008-03-17 2009-03-17 Combined chemical and genetic approaches for generation of induced pluripotent stem cells SG10201808863UA (en)

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SG2013019211A SG188904A1 (en) 2008-03-17 2009-03-17 Combined chemical and genetic approaches for generation of induced pluripotent stem cells
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KR (1) KR101679082B1 (en)
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Families Citing this family (174)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110171185A1 (en) * 1999-06-30 2011-07-14 Klimanskaya Irina V Genetically intact induced pluripotent cells or transdifferentiated cells and methods for the production thereof
US7682828B2 (en) 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
EP2302034B1 (en) * 2005-08-03 2020-10-14 Astellas Institute for Regenerative Medicine Improved methods of reprogramming animal somatic cells
EP1942739B1 (en) 2005-10-18 2010-12-08 National Jewish Health Conditionally immortalized long-term adult stem cells and methods of making and using such cells
US8129187B2 (en) 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
EP2206724A1 (en) * 2005-12-13 2010-07-14 Kyoto University Nuclear reprogramming factor
US20090227032A1 (en) * 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
US8278104B2 (en) * 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
CA2683056C (en) 2007-04-07 2020-03-24 Whitehead Institute For Biomedical Research Reprogramming of somatic cells
JP2008307007A (en) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag Human pluripotent stem cell induced from human tissue-originated undifferentiated stem cell after birth
US9213999B2 (en) * 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
US20140342984A1 (en) * 2007-10-19 2014-11-20 Robert G. Matheny Compositions for Regenerating Defective or Absent Myocardium
ES2690554T3 (en) 2008-03-17 2018-11-21 The Scripps Research Institute Combined chemical and genetic approaches for the generation of induced pluripotent stem cells
EP2276834A4 (en) * 2008-04-07 2012-02-08 Nupotential Inc Reprogramming a cell by inducing a pluripotent gene through use of a small molecule modulator
US20100279404A1 (en) 2008-05-02 2010-11-04 Shinya Yamanaka Method of nuclear reprogramming
SG10201803982TA (en) 2008-05-16 2018-07-30 Taiga Biotechnologies Inc Antibodies and processes for preparing the same
EP2300611B1 (en) 2008-06-13 2017-08-09 Whitehead Institute for Biomedical Research Programming and reprogramming of cells
EP2746387A1 (en) 2008-07-21 2014-06-25 Taiga Biotechnologies, Inc. Differentiated anucleated cells and method for preparing the same
US8298825B1 (en) * 2008-08-25 2012-10-30 The General Hospital Corporation TGF-beta receptor inhibitors to enhance direct reprogramming
DK2318435T3 (en) 2008-08-28 2016-02-29 Taiga Biotechnologies Inc Modulators of MYC, methods of using the same, and methods for identifying agents that modulate MYC
US9045737B2 (en) * 2008-12-13 2015-06-02 Dnamicroarray, Inc. Artificial three-dimensional microenvironment niche culture
EP2373784B1 (en) 2008-12-17 2017-10-25 The Scripps Research Institute Generation and maintenance of stem cells
EP2641911A3 (en) * 2008-12-23 2014-01-01 Vivoscript, Inc. Compositions and methods for re-programming cells without genetic modification
WO2010115052A2 (en) * 2009-04-03 2010-10-07 The Mclean Hospital Corporation Induced pluripotent stem cells
US9109245B2 (en) 2009-04-22 2015-08-18 Viacyte, Inc. Cell compositions derived from dedifferentiated reprogrammed cells
JP5777113B2 (en) 2009-05-29 2015-09-09 学校法人慶應義塾 Method for selecting induced pluripotent stem cell clones
JP5751548B2 (en) * 2009-08-07 2015-07-22 国立大学法人京都大学 Canine iPS cells and production method thereof
AU2014250681B2 (en) * 2009-10-16 2016-12-15 The Scripps Research Institute Induction of pluripotent cells
BR112012008848A2 (en) * 2009-10-16 2019-09-24 Scripps Research Inst in vitro or ex vivo method for inducing non-pluripotent mammalian cells into induced pluripotent stem cells
AU2010312291A1 (en) 2009-10-29 2012-06-21 Mcmaster University Generating induced pluripotent stem cells and progenitor cells from fibroblasts
SG10201702120VA (en) * 2009-10-31 2017-05-30 Genesis Technologies Ltd Methods for Reprogramming Cells and Uses Thereof
WO2011056971A2 (en) * 2009-11-04 2011-05-12 Cellular Dynamics International, Inc. Episomal reprogramming with chemicals
WO2011082038A2 (en) * 2009-12-31 2011-07-07 Fate Therapeutics, Inc. Improved reprogramming compositions
US20130059385A1 (en) * 2010-03-02 2013-03-07 The Scripps Research Institute Methods of generating pluripotent stem cells
EP2366432A1 (en) * 2010-03-16 2011-09-21 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Inhibitors of glycogen synthase kinase 3 for use in therapeutic methods and screening method relating thereto
JP5909482B2 (en) 2010-03-31 2016-04-26 ザ スクリプス リサーチ インスティテュート Cell reprogramming
CN102260646B (en) * 2010-05-26 2015-09-16 北京瑞普晨创科技有限公司 Prepare a method for induced pluripotent stem cells, test kit and purposes
US9376664B2 (en) 2010-06-14 2016-06-28 The Scripps Research Institute Reprogramming of cells to a new fate
KR101861171B1 (en) 2010-06-18 2018-05-31 후지필름 셀룰러 다이내믹스, 인코포레이티드 Cardiomyocyte medium with dialyzed serum
WO2012007725A2 (en) * 2010-07-16 2012-01-19 Plasticell Ltd Method of reprogramming a cell
WO2012030854A2 (en) 2010-09-01 2012-03-08 Whitehead Institute For Biomedical Research Compositions and methods for modulating emt and uses thereof
US20120064041A1 (en) * 2010-09-03 2012-03-15 Arshak Alexanian Efficient Generation of Neurally-Induced Mesenchymal Stem Cells and Applications Thereof
EP2616540A4 (en) * 2010-09-14 2014-02-19 Univ Kyoto Method of efficiently establishing induced pluripotent stem cells
EP2618829B1 (en) * 2010-09-22 2019-05-01 The Regents of the University of Colorado, a body corporate Smad7 for use in the treatment of oral mucositis or psoriasis
CN103384679B (en) * 2010-12-17 2015-08-26 拜奥拉米那公司 Restructuring Kallinin 21
WO2012087965A2 (en) 2010-12-22 2012-06-28 Fate Therapauetics, Inc. Cell culture platform for single cell sorting and enhanced reprogramming of ipscs
EP2660314A4 (en) * 2010-12-27 2014-09-03 Lsip Llc iPS CELLS AND METHOD FOR GENERATING SAME
WO2012098260A1 (en) * 2011-01-21 2012-07-26 Axiogenesis Ag A non-viral system for the generation of induced pluripotent stem (ips) cells
ES2957478T3 (en) 2011-05-02 2024-01-19 Univ Wayne State A protein-induced pluripotent stem cell technology and uses thereof
WO2012166973A1 (en) * 2011-06-01 2012-12-06 Sanford-Burnham Medical Research Institute Methods for promoting cell reprogramming
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
JP6113160B2 (en) * 2011-07-19 2017-04-12 ヴィヴォスクリプト,インコーポレイテッド Compositions and methods for reprogramming cells without genetic modification to repair cartilage damage
CN103703130A (en) 2011-07-25 2014-04-02 国立大学法人京都大学 Method for screening induced pluripotent stem cells
CN103917641B (en) 2011-10-21 2018-04-27 爱科来株式会社 The cultivation of the single cell dispersion of the maintenance versatility carried out by laminar flow
WO2013077423A1 (en) 2011-11-25 2013-05-30 国立大学法人京都大学 Method for culturing pluripotent stem cell
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
WO2013086008A1 (en) 2011-12-05 2013-06-13 Factor Bioscience Inc. Methods and products for transfecting cells
US20150017134A1 (en) * 2012-03-01 2015-01-15 Whitehead Institute For Biomedical Research Emt-inducing transcription factors cooperate with sox9
EP2825637A4 (en) * 2012-03-14 2015-12-23 Childrens Medical Center High-throughput image-based chemical screening in zebrafish blastomere cell culture
EP3868387A1 (en) 2012-07-20 2021-08-25 Taiga Biotechnologies, Inc. Enhanced reconstitution and autoreconstitution of the hematopoietic compartment
US10357517B1 (en) * 2012-10-01 2019-07-23 University Of South Florida Methods of treating epilepsy using neural stem cells that express nanog, SSEA-4, OCT-4, MIR-34B, MIR-34C and MIR-592
WO2014071219A1 (en) 2012-11-01 2014-05-08 Factor Bioscience Inc. Methods and products for expressing proteins in cells
US10130637B2 (en) 2012-11-02 2018-11-20 Duke University Inhibition of histone methyltransferase for cardiac reprogramming
JP6509126B2 (en) 2013-01-09 2019-05-08 インターナショナル ステム セル コーポレイション Small molecules that promote skin regeneration
EP2955223B1 (en) 2013-02-08 2019-12-18 Kyoto University Production methods for megakaryocytes and platelets
JP6292415B2 (en) 2013-03-06 2018-03-14 国立大学法人京都大学 Pluripotent stem cell culture system and pluripotent stem cell passage method
EP2964774B1 (en) 2013-03-08 2020-05-06 The Regents of The University of Colorado, A Body Corporate Ptd-smad7 therapeutics
US10272115B2 (en) 2013-03-11 2019-04-30 Taiga Biotechnologies, Inc. Production and use of red blood cells
US9365825B2 (en) * 2013-03-11 2016-06-14 Taiga Biotechnologies, Inc. Expansion of adult stem cells in vitro
DK2970890T3 (en) 2013-03-14 2020-05-04 Brigham & Womens Hospital Inc COMPOSITIONS AND PROCEDURES FOR THE PROMOTION AND CULTIVATION OF EPITHEL STEM CELLS
US8859286B2 (en) 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
WO2014148646A1 (en) 2013-03-21 2014-09-25 国立大学法人京都大学 Pluripotent stem cell for neuronal differentiation induction
JP6473686B2 (en) 2013-03-25 2019-02-20 公益財団法人神戸医療産業都市推進機構 Cell sorting method
KR20140121317A (en) * 2013-04-06 2014-10-15 서울대학교산학협력단 Method for producing induced neural stem cell from non-neuronal cell, and induced neural stem cell produced by the same
JP6461787B2 (en) 2013-04-12 2019-01-30 国立大学法人京都大学 Method for inducing alveolar epithelial progenitor cells
JP6429280B2 (en) 2013-05-14 2018-11-28 国立大学法人京都大学 Efficient cardiomyocyte induction method
US10159766B2 (en) 2013-05-31 2018-12-25 Iheart Japan Corporation Layered cell sheet incorporating hydrogel
WO2014200115A1 (en) 2013-06-11 2014-12-18 国立大学法人京都大学 Method for producing renal precursor cells, and drug containing renal precursor cells
CN104278008B (en) * 2013-07-12 2020-08-21 北京宏冠再生医学科技有限公司 Method, kit and application for preparing pluripotent stem cells through small molecule compound treatment
KR101655383B1 (en) 2013-07-27 2016-09-08 고려대학교 산학협력단 Composition for Maintaining Chromosome Stability of Pluripotent Stem Cells Comprising Small Molecules
JP6378183B2 (en) 2013-08-07 2018-08-22 国立大学法人京都大学 Method for producing pancreatic hormone-producing cells
KR102320537B1 (en) 2013-09-05 2021-11-01 고쿠리츠 다이가쿠 호진 교토 다이가쿠 New method for inducing dopamine-producing neural precursor cells
CN104419683A (en) * 2013-09-10 2015-03-18 中国科学院生物物理研究所 Method for preparing autologous induced pluripotent stem cells of patient with Fanconi anemia and application of method
US10144915B2 (en) 2013-10-23 2018-12-04 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Reprogramming fibroblasts into cardiomyocytes
JP6415582B2 (en) 2013-10-25 2018-10-31 ウェイン ステート ユニバーシティー Methods, systems, and compositions for cell transformation by protein-induced in vivo cell reprogramming
KR102219743B1 (en) 2013-11-01 2021-02-23 고쿠리츠 다이가쿠 호진 교토 다이가쿠 Novel chondrocyte induction method
US9512406B2 (en) 2013-12-20 2016-12-06 The J. David Gladstone Institute, a testamentary trust established under the Will of J. David Gladstone Generating hepatocytes
MX2016009771A (en) 2014-01-31 2016-11-14 Factor Bioscience Inc Methods and products for nucleic acid production and delivery.
CN104894060B (en) * 2014-03-03 2018-11-06 中国科学院上海生命科学研究院 Inducing somatic transdifferentiation is the method and its application of neural stem cell
EP3114214B1 (en) 2014-03-04 2023-11-01 Fate Therapeutics, Inc. Improved reprogramming methods and cell culture platforms
JP7012432B2 (en) 2014-07-14 2022-01-28 中外製薬株式会社 Methods for identifying protein epitopes
US20170211091A1 (en) * 2014-07-16 2017-07-27 Fundación Pública Andaluza Progreso Y Salud Methods for generating induced pluripotent stem cells
WO2016022472A1 (en) * 2014-08-04 2016-02-11 University Of Kansas Mammalian pluripotent stem cells, methods for their production, and uses thereof
RU2565548C1 (en) * 2014-08-05 2015-10-20 Дмитрий Андреевич Журавлёв Method of producing of pluripotent stem cells
WO2016022930A1 (en) 2014-08-07 2016-02-11 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Reversible stencils for fabricating micro-tissues
US10568883B2 (en) * 2014-09-03 2020-02-25 Massachusetts Institute Of Technology Compositions, systems, and methods for generating inner ear hair cells for treatment of hearing loss
US10711249B2 (en) 2014-12-26 2020-07-14 Kyoto University Method for inducing hepatocytes
WO2016117139A1 (en) * 2015-01-20 2016-07-28 タカラバイオ株式会社 Method for producing nervous system cells
US20180112180A1 (en) 2015-01-26 2018-04-26 Fate Therapeutics, Inc. Cells with increased immuno-regulatory properties and methods for their use and manufacture
US10767165B2 (en) * 2015-01-30 2020-09-08 Centre National De La Recherche Scientifique (Cnrs) Reprogramming method for producing induced pluripotent stem cells (iPSC)
AU2016218977C1 (en) 2015-02-13 2023-03-23 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
JP2018510649A (en) 2015-02-17 2018-04-19 ユニバーシティー ヘルス ネットワーク Methods for making and using sinoatrial node-like pacemaker cardiomyocytes and ventricular cardiomyocytes
JP2016202172A (en) 2015-04-16 2016-12-08 国立大学法人京都大学 Production method of pseudoislet
KR20180033537A (en) 2015-07-21 2018-04-03 더 칠드런스 메디칼 센터 코포레이션 PD-L1 expressing hematopoietic stem cells and uses
CN108350433B (en) * 2015-08-21 2021-11-30 京都府公立大学法人 Osteoblast and method for producing the same
CN117737124A (en) * 2015-10-16 2024-03-22 菲特治疗公司 Platform for inducing and maintaining ground state pluripotency
CN116024164A (en) * 2015-10-21 2023-04-28 京都府公立大学法人 Method for producing cells
EP3400286A1 (en) 2016-01-08 2018-11-14 Massachusetts Institute Of Technology Production of differentiated enteroendocrine cells and insulin producing cells
EP3401392B1 (en) * 2016-01-08 2022-02-23 Evia Life Sciences Inc. Method for producing hepatic stem/precursor cells from mature hepatic cells using low-molecular-weight compound
US11096964B2 (en) * 2016-01-20 2021-08-24 Fate Therapeutics, Inc. Compositions and methods for immune cell modulation in adoptive immunotherapies
US11413309B2 (en) 2016-01-20 2022-08-16 Fate Therapeutics, Inc. Compositions and methods for immune cell modulation in adoptive immunotherapies
US10213511B2 (en) 2016-03-02 2019-02-26 Frequency Therapeutics, Inc. Thermoreversible compositions for administration of therapeutic agents
US11260130B2 (en) 2016-03-02 2022-03-01 Frequency Therapeutics, Inc. Solubilized compositions for controlled proliferation of stem cells / generating inner ear hair cells using a GSK3 inhibitor: IV
US10201540B2 (en) 2016-03-02 2019-02-12 Frequency Therapeutics, Inc. Solubilized compositions for controlled proliferation of stem cells / generating inner ear hair cells using GSK3 inhibitors: I
PT3444334T (en) 2016-04-15 2021-09-08 Univ Kyoto Method for inducing cd8+ t cells
JP7011260B2 (en) 2016-04-22 2022-02-10 国立大学法人京都大学 Method for producing dopamine-producing neural progenitor cells
CN105943526B (en) * 2016-05-18 2018-08-14 上海交通大学医学院附属上海儿童医学中心 Applications of the PD0325901 in maintaining arterial duct to open
EP3483262B1 (en) * 2016-06-03 2021-11-17 Institue of Transfusion Medicine, Academy of Military Medical Sciences, People's Liberation Army of China Small molecule compound combination for reprogramming digestive tract derived epithelial cells to endodermal stem/progenitor cells, reprogramming method and application
WO2018005799A1 (en) * 2016-06-29 2018-01-04 Georgia State University Research Foundation, Inc. Histone deacetylase and histone methyltransferase inhibitors and methods of making and use of the same
CA3033788A1 (en) 2016-08-17 2018-02-22 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
EP3533865A4 (en) * 2016-10-28 2020-04-15 National Cancer Center Method for preparing human hepatocyte progenitor cells
CN113786476A (en) 2016-12-02 2021-12-14 泰加生物工艺学公司 Nanoparticle formulations
WO2018106595A1 (en) 2016-12-05 2018-06-14 Fate Therapeutics, Inc. Compositions and methods for immune cell modulation in adoptive immunotherapies
KR102537361B1 (en) 2016-12-27 2023-05-26 스미또모 가가꾸 가부시키가이샤 Methods for evaluating and selecting artificial pluripotent stem cells, and methods for producing artificial pluripotent stem cells
CN106754655B (en) * 2016-12-29 2020-06-19 赛业(苏州)生物科技有限公司 Animal embryo culture medium
MX2019007890A (en) 2016-12-30 2020-01-20 Frequency Therapeutics Inc 1h-pyrrole-2,5-dione compounds and methods of using them to induce self-renewal of stem/progenitor supporting cells.
CN110199017A (en) 2017-01-20 2019-09-03 国立大学法人京都大学 CD8 alpha+beta+cytotoxic T cell preparation method
JP7162537B2 (en) 2017-01-26 2022-10-28 国立大学法人大阪大学 Medium for inducing differentiation of stem cells into mesodermal cells and method for producing mesodermal cells
WO2018168829A1 (en) 2017-03-14 2018-09-20 国立大学法人京都大学 Method for producing helper t cells from pluripotent stem cells
KR20230150412A (en) 2017-05-25 2023-10-30 고쿠리츠 다이가쿠 호진 교토 다이가쿠 Method for inducing differentiation of intermediate mesodermal cell to renal progenitor cell, and method for inducing differentiation of pluripotent stem cell to renal progenitor cell
KR102422175B1 (en) 2017-06-19 2022-07-18 고에키 자이단 호징 고베 이료 산교 도시 스이신 기코 Method for predicting differentiation capacity of pluripotent stem cells and reagents therefor
US10149898B2 (en) 2017-08-03 2018-12-11 Taiga Biotechnologies, Inc. Methods and compositions for the treatment of melanoma
US20190062711A1 (en) * 2017-08-25 2019-02-28 So Young Life Sciences Corporation Use Of Phosphorylated TBeta4 And Other Factors To Generate Human Induced Pluripotent Stem Cells
WO2019060708A1 (en) 2017-09-22 2019-03-28 The Children's Medical Center Corporation Treatment of type 1 diabetes and autoimmune diseases or disorders
EP3699267A4 (en) 2017-10-17 2021-10-27 Kyoto University Method for obtaining artificial neuromuscular junction from pluripotent stem cells
KR101810014B1 (en) 2017-10-23 2018-01-18 차의과학대학교 산학협력단 Method for generating induced pluripotent stem cells from human-derived somatic cells using histone deacetylase inhibitor and bone morphogenetic protein pathway blocker
KR101810008B1 (en) 2017-10-23 2018-01-18 차의과학대학교 산학협력단 Method for generating induced pluripotent stem cells from human-derived somatic cells using reprogramming-enhancing agents
KR101998633B1 (en) * 2017-10-31 2019-07-11 가톨릭관동대학교산학협력단 Use of ellipticine for inducing differentiation of stem cells into chondrocytes
CN108624562B (en) * 2018-05-18 2021-11-02 首都医科大学附属北京朝阳医院 Method for preparing tumor stem cells and special kit thereof
KR102158783B1 (en) * 2018-06-18 2020-09-22 한국생명공학연구원 Cell reprogramming composition and method for producing an indueced pluripotent stem cell using the same
BR112021000437A2 (en) 2018-07-13 2021-04-06 Kyoto University METHODS FOR PRODUCING A DELTA RANGE T CELL AND FOR PREVENTING OR TREATING TUMOR, DELTA RANGE T CELL, CELL POPULATION, MEDICINE, EXTERMINATION AGENT FOR A CELL, AND, USE OF THE CELL
JP7285015B2 (en) 2018-07-19 2023-06-01 国立大学法人京都大学 Lamellar cartilage derived from pluripotent stem cells and method for producing the same
US20210332329A1 (en) 2018-07-23 2021-10-28 Kyoto University Novel renal progenitor cell marker and method for concentrating renal progenitor cells using same
WO2020037326A1 (en) 2018-08-17 2020-02-20 Frequency Therapeutics, Inc. Compositions and methods for generating hair cells by downregulating foxo
CA3109647A1 (en) 2018-08-17 2020-02-20 Frequency Therapeutics, Inc. Compositions and methods for generating hair cells by upregulating jag-1
WO2020116606A1 (en) 2018-12-06 2020-06-11 キリンホールディングス株式会社 Production method for t cells or nk cells, medium for culturing t cells or nk cells, method for culturing t cells or nk cells, method for maintaining undifferentiated state of undifferentiated t cells, and growth-accelerating agent for t cells or nk cells
US20220056413A1 (en) 2018-12-21 2022-02-24 Kyoto University Lubricin-localized cartilage-like tissue, method for producing same and composition comprising same for treating articular cartilage damage
US20220062341A1 (en) 2018-12-26 2022-03-03 Kirin Holdings Kabushiki Kaisha Modified tcr and production method therefor
CN113811316A (en) 2019-05-15 2021-12-17 味之素株式会社 Method for purifying neural crest cells or corneal epithelial cells
EP3974519A4 (en) 2019-05-20 2023-07-12 Ajinomoto Co., Inc. Expansion culture method for cartilage or bone precursor cells
WO2021015228A1 (en) * 2019-07-25 2021-01-28 富士フイルム株式会社 Method for producing specific differentiated cells
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods
CN112301052A (en) * 2019-08-01 2021-02-02 浙江霍德生物工程有限公司 Method for preparing induced multifunctional stem cells through somatic cell reprogramming
CN115023233A (en) 2019-12-12 2022-09-06 国立大学法人千叶大学 Freeze-dried preparation comprising megakaryocytes and platelets
KR102087084B1 (en) * 2020-02-03 2020-03-10 주식회사 이에이치엘바이오 Culture method of adipose-drived stem cell proliferation expressing anti-aging factors
KR20220143063A (en) 2020-02-19 2022-10-24 남미 테라퓨틱스, 인크. Formulated and/or co-formulated liposome compositions containing TGFβ antagonist prodrugs useful for the treatment of cancer and methods thereof
MX2022010521A (en) 2020-02-28 2022-09-19 Takeda Pharmaceuticals Co Method for producing natural killer cells from pluripotent stem cells.
US20230257705A1 (en) 2020-06-17 2023-08-17 Kyoto University Chimeric antigen receptor-expressing immunocompetent cells
WO2022014604A1 (en) 2020-07-13 2022-01-20 国立大学法人京都大学 Skeletal muscle precursor cells and method for purifying same, composition for treating myogenic diseases, and method for producing cell group containing skeletal muscle precursor cells
JPWO2022019152A1 (en) 2020-07-20 2022-01-27
EP4202041A1 (en) 2020-08-18 2023-06-28 Kyoto University Method for maintaining and amplifying human primordial germ cells / human primordial germ cell-like cells
WO2022196714A1 (en) 2021-03-17 2022-09-22 アステラス製薬株式会社 Pericyte having basic fibroblast growth factor (bfgf) gene introduced therein
TW202305112A (en) 2021-04-30 2023-02-01 國立研究開發法人理化學研究所 Cord-like aggregates of retinal pigment epithelial cells, device and production method for producing same, and therapeutic agent comprising said cord-like aggregates
WO2022255489A1 (en) 2021-06-04 2022-12-08 キリンホールディングス株式会社 Cell composition, method for producing cell composition, and pharmaceutical composition containing cell composition
CA3222761A1 (en) 2021-06-10 2022-12-15 Ajinomoto Co., Inc. Method for producing mesenchymal stem cells
AR126145A1 (en) 2021-06-15 2023-09-13 Takeda Pharmaceuticals Co METHOD FOR PRODUCING NATURAL CYTOLYTIC LYMPHOCYTES FROM PLURIPOTENTIVE STEM CELLS
WO2023283631A2 (en) * 2021-07-08 2023-01-12 The Broad Institute, Inc. Methods for differentiating and screening stem cells
WO2023286834A1 (en) 2021-07-15 2023-01-19 アステラス製薬株式会社 Pericyte-like cell expressing vascular endothelial growth factor (vegf) at high level
JPWO2023286832A1 (en) 2021-07-15 2023-01-19
WO2023017848A1 (en) 2021-08-11 2023-02-16 国立大学法人京都大学 Method for producing renal interstitial progenitor cells, erythropoietin-producing cells, and method for producing renin-producing cells
WO2023074648A1 (en) * 2021-10-25 2023-05-04 富士フイルム株式会社 Method for producing intestinal endocrine cells, pluripotent stem cell-derived intestinal endocrine cells, medium or medium kit, and use of the same
CN116694551A (en) * 2022-02-24 2023-09-05 清华大学 Induced mature liver cell and preparation method thereof
CN114702591B (en) * 2022-05-17 2022-09-23 诺赛联合(北京)生物医学科技有限公司 Preparation technology of adult cell-derived organoid
JP7461691B1 (en) 2023-06-23 2024-04-04 株式会社アンプリー Carbon dioxide gas pack kit

Family Cites Families (105)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR901228A (en) 1943-01-16 1945-07-20 Deutsche Edelstahlwerke Ag Ring gap magnet system
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US5506337A (en) 1985-03-15 1996-04-09 Antivirals Inc. Morpholino-subunit combinatorial library and method
US5139941A (en) 1985-10-31 1992-08-18 University Of Florida Research Foundation, Inc. AAV transduction vectors
US5010175A (en) 1988-05-02 1991-04-23 The Regents Of The University Of California General method for producing and selecting peptides with specific properties
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
IE66205B1 (en) 1990-06-14 1995-12-13 Paul A Bartlett Polypeptide analogs
US5650489A (en) 1990-07-02 1997-07-22 The Arizona Board Of Regents Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
AU2515992A (en) 1991-08-20 1993-03-16 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
US5702932A (en) 1992-07-20 1997-12-30 University Of Florida Microinjection methods to transform arthropods with exogenous DNA
US5288514A (en) 1992-09-14 1994-02-22 The Regents Of The University Of California Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support
EP0710283A4 (en) * 1993-07-22 1997-07-02 Merck & Co Inc EXPRESSION OF HUMAN INTERLEUKIN-1-g(b) IN A TRANSGENIC ANIMAL
US5519134A (en) 1994-01-11 1996-05-21 Isis Pharmaceuticals, Inc. Pyrrolidine-containing monomers and oligomers
US5593853A (en) 1994-02-09 1997-01-14 Martek Corporation Generation and screening of synthetic drug libraries
US5539083A (en) 1994-02-23 1996-07-23 Isis Pharmaceuticals, Inc. Peptide nucleic acid combinatorial libraries and improved methods of synthesis
US5656610A (en) 1994-06-21 1997-08-12 University Of Southern California Producing a protein in a mammal by injection of a DNA-sequence into the tongue
US5525735A (en) 1994-06-22 1996-06-11 Affymax Technologies Nv Methods for synthesizing diverse collections of pyrrolidine compounds
US5549974A (en) 1994-06-23 1996-08-27 Affymax Technologies Nv Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof
US5736524A (en) 1994-11-14 1998-04-07 Merck & Co.,. Inc. Polynucleotide tuberculosis vaccine
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5569588A (en) 1995-08-09 1996-10-29 The Regents Of The University Of California Methods for drug screening
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
US5780448A (en) 1995-11-07 1998-07-14 Ottawa Civic Hospital Loeb Research DNA-based vaccination of fish
GB9807935D0 (en) 1998-04-14 1998-06-10 Univ Edinburgh Lineage specific cells and progenitor cells
US5945100A (en) 1996-07-31 1999-08-31 Fbp Corporation Tumor delivery vehicles
US5981274A (en) 1996-09-18 1999-11-09 Tyrrell; D. Lorne J. Recombinant hepatitis virus vectors
ATE421329T1 (en) 1997-05-07 2009-02-15 Pharma Mar Sa USE OF APLIDINE TO TREAT CARDIOVASCULAR DISEASES
US6270994B1 (en) 1997-06-13 2001-08-07 Japanese Foundation For Cancer Research Smad6 and uses thereof
US5994624A (en) 1997-10-20 1999-11-30 Cotton Incorporated In planta method for the production of transgenic plants
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
US20060263382A1 (en) 1998-06-20 2006-11-23 Richard Hotchkiss Membrane-permeant peptide complexes for treatment of sepsis
US6800790B2 (en) * 1998-07-24 2004-10-05 Carnegie Institution Of Washington Method for maintenance and propagation of germline stem cells using members of the TFG-β family of growth factors
GB9819912D0 (en) * 1998-09-11 1998-11-04 Univ Edinburgh Propagation and/or derivation of embryonic stem cells
WO2000040235A2 (en) 1999-01-07 2000-07-13 Warner-Lambert Company Treatment of asthma with mek inhibitors
US6703420B1 (en) 1999-03-19 2004-03-09 Bristol-Myers Squibb Pharma Company Amino-thio-acrylonitriles as MEK inhibitors
US6730293B1 (en) 1999-08-24 2004-05-04 Cellgate, Inc. Compositions and methods for treating inflammatory diseases of the skin
US20020142457A1 (en) * 1999-12-28 2002-10-03 Akihiro Umezawa Cell having the potentiality of differentiation into cardiomyocytes
US20030180947A1 (en) * 2001-09-21 2003-09-25 J.H. David Wu Circadian control of stem/progenitor cell self-renewal and differentiation and of clock controlled gene expression
ES2388968T3 (en) 2001-11-02 2012-10-22 Giuliani International Limited Smad7 inhibitors for the treatment of CNS diseases
EP1480515B1 (en) * 2002-03-05 2005-10-19 ARTEMIS Pharmaceuticals GmbH Inbred embryonic stem-cell derived mice
GB0210539D0 (en) 2002-05-08 2002-06-19 Univ Edinburgh Control of es cell self renewal and lineage specification, and medium therefor
MXPA05003431A (en) 2002-11-15 2005-07-05 Warner Lambert Co Combination chemotherapy comprising a mek inhibitor and capecitabine for treating cancer.
CA2515215A1 (en) * 2003-02-10 2004-08-26 Amgen Inc. Vanilloid receptor ligands and their use in treatments
AU2003901099A0 (en) 2003-03-11 2003-03-27 Es Cell International Pte Ltd. Methods of inducing differentiation of stem cells
US20070010008A1 (en) * 2005-06-29 2007-01-11 Tissuetech, Inc. Ex vivo expansion of primary animal cells
EP1732581A4 (en) 2003-06-20 2008-06-04 Univ California San Diego Polypeptide transduction and fusogenic peptides
CN103602633A (en) * 2003-10-03 2014-02-26 福田惠一 Method of inducing the differentiation of stem cells into myocardial cells
GB2407048B (en) 2003-10-15 2007-07-11 Biotrace Internat Plc Laboratory apparatus
JP2007508026A (en) 2003-10-16 2007-04-05 ザ・ユニバーシティ・コート・オブ・ザ・ユニバーシティ・オブ・エディンバラ Control of self-renewal and lineage specification of ES cells and culture medium therefor
AU2004291559B2 (en) 2003-11-19 2008-10-16 Australian Stem Cell Centre Limited Methods for producing blood products from pluripotent cells in cell culture
US20070269412A1 (en) 2003-12-02 2007-11-22 Celavie Biosciences, Llc Pluripotent cells
JP5102030B2 (en) 2004-08-13 2012-12-19 ユニバーシティ・オブ・ジョージア・リサーチ・ファウンデイション・インコーポレイテッド Compositions and methods for self-renewal and differentiation in human embryonic stem cells
US20080268533A1 (en) * 2004-08-25 2008-10-30 University Of Georgia Research Foundation, Inc. Methods and Compositions Utilizing Myc and Gsk3Beta to Manipulate the Pluripotency of Embryonic Stem Cells
WO2006088867A2 (en) * 2005-02-15 2006-08-24 Medistem Laboratories, Incorporated Method for expansion of stem cells
CN101313065A (en) 2005-08-01 2008-11-26 纽珀滕索有限公司 Production of reprogrammed cells with restored potential
WO2007030693A2 (en) 2005-09-08 2007-03-15 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Methods for promoting stem cell proliferation and survival
BRPI0619704A2 (en) * 2005-11-03 2011-10-11 Vertex Pharma compound, composition, method for inhibiting aurora kinase activity in a biological sample, method for treating a proliferative disorder and method for treating cancer
US8217042B2 (en) * 2005-11-11 2012-07-10 Zentaris Gmbh Pyridopyrazines and their use as modulators of kinases
JP2009515515A (en) * 2005-11-11 2009-04-16 ザ・ユニバーシティ・コート・オブ・ザ・ユニバーシティ・オブ・エディンバラ Cell reprogramming and genetic modification
US20090227032A1 (en) * 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
EP2206724A1 (en) * 2005-12-13 2010-07-14 Kyoto University Nuclear reprogramming factor
WO2007069686A1 (en) 2005-12-16 2007-06-21 Meidensha Corporation Vacuum capacitor and vacuum valve
GB0601538D0 (en) * 2006-01-26 2006-03-08 Univ Birmingham Epigenetic analysis
JPWO2007088651A1 (en) 2006-02-01 2009-06-25 国立大学法人 東京大学 Combination use of TGF-β signal inhibitor and antitumor agent
US7541186B2 (en) 2006-02-22 2009-06-02 University Of Washington Method of generating human retinal progenitors from embryonic stem cells
GB0615327D0 (en) 2006-03-30 2006-09-13 Univ Edinburgh Culture medium containing kinase inhibitors and uses thereof
TW200815597A (en) 2006-03-30 2008-04-01 Univ Edinburgh Culture medium containing kinase inhibitors, and uses thereof
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
US20070259423A1 (en) 2006-05-02 2007-11-08 Jon Odorico Method of differentiating stem cells into cells of the endoderm and pancreatic lineage
GB2437737B (en) 2006-05-04 2008-07-09 Access Products Ltd Scaffolding
US7884107B2 (en) 2006-06-30 2011-02-08 Merck Substituted piperidines that increase P53 activity and the uses thereof
AU2007271964B2 (en) 2006-07-14 2012-01-19 Novartis Ag Pyrimidine derivatives as ALK-5 inhibitors
US20080020014A1 (en) 2006-07-19 2008-01-24 Paul Consigny Implantable devices containing nuclear receptor ligands for the treatment of vascular and related disorders
GB0622395D0 (en) 2006-11-09 2006-12-20 Univ Cambridge Tech Methods relating to pluripotent cells
JP5419279B2 (en) 2007-01-17 2014-02-19 ウィスコンシン アラムニ リサーチ ファンデーション Improved stem cell culture
WO2008088882A2 (en) 2007-01-19 2008-07-24 The J. David Gladstone Institutes Methods of generating cardiomyocytes
CN103627671A (en) 2007-01-30 2014-03-12 佐治亚大学研究基金会 Method for generating endoderm and mesoderm lineages and multipotent migratory cells (MMC), and cell population and use
WO2008105630A1 (en) 2007-02-27 2008-09-04 Procell Therapeutics Inc. Combined use of cell permeable nanog and oct4 for increasing self-renewal and suppressing differentiation of stem cells
EP3449923A1 (en) 2007-03-07 2019-03-06 MEI Pharma, Inc. Combination of benzimidazole anti-cancer agent and a second anti-cancer agent
WO2008126932A2 (en) 2007-04-09 2008-10-23 Riken Epigenetical regulation of brain plasticity
EP2147094B2 (en) * 2007-04-18 2018-02-21 Hadasit Medical Research Services & Development Limited Stem cell-derived retinal pigment epithelial cells
US8648069B2 (en) * 2007-06-08 2014-02-11 Abbvie Inc. 5-substituted indazoles as kinase inhibitors
JP2008307007A (en) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag Human pluripotent stem cell induced from human tissue-originated undifferentiated stem cell after birth
CA2691793A1 (en) 2007-06-29 2009-01-08 Cellular Dynamics International, Inc. Automated method and apparatus for embryonic stem cell culture
WO2009032456A2 (en) 2007-08-01 2009-03-12 Primegen Biotech Llc Non-viral delivery of transcription factors that reprogram human somatic cells into a stem cell-like state
CN101855338B (en) * 2007-08-31 2013-07-17 怀特黑德生物医学研究所 WNT pathway stimulation in reprogramming somatic cells
AU2008297024B2 (en) * 2007-10-31 2014-08-28 Kyoto University Nuclear reprogramming method
JP4604076B2 (en) * 2007-11-07 2010-12-22 国立大学法人静岡大学 Electrolyte membrane for fuel cell
WO2009073523A2 (en) 2007-11-29 2009-06-11 Children's Hospital Of Orange County De-differentiation of human cells
AU2008329563A1 (en) 2007-11-30 2009-06-04 Cytomatrix Pty Ltd Methods of inducing pluripotency involving Oct4 protein
AU2008329562A1 (en) 2007-11-30 2009-06-04 Cytomatrix Pty Ltd Methods of inducing pluripotency involving Sox2 protein
WO2009082184A2 (en) 2007-12-26 2009-07-02 Posdata Co., Ltd. Method for determining spurious and wireless communication system applying that method
ES2690554T3 (en) * 2008-03-17 2018-11-21 The Scripps Research Institute Combined chemical and genetic approaches for the generation of induced pluripotent stem cells
US8298825B1 (en) * 2008-08-25 2012-10-30 The General Hospital Corporation TGF-beta receptor inhibitors to enhance direct reprogramming
EP2373784B1 (en) 2008-12-17 2017-10-25 The Scripps Research Institute Generation and maintenance of stem cells
WO2010102267A2 (en) * 2009-03-06 2010-09-10 Ipierian, Inc. Tgf-beta pathway inhibitors for enhancement of cellular reprogramming of human cells
JP5777113B2 (en) * 2009-05-29 2015-09-09 学校法人慶應義塾 Method for selecting induced pluripotent stem cell clones
AU2010279913B2 (en) * 2009-08-07 2016-04-28 Kyoto University Method of efficiently establishing induced pluripotent stem cells
BR112012008848A2 (en) 2009-10-16 2019-09-24 Scripps Research Inst in vitro or ex vivo method for inducing non-pluripotent mammalian cells into induced pluripotent stem cells
ES2533581T3 (en) 2010-03-05 2015-04-13 Texas Heart Institute ETS2 and Mesp1 generators of cardiac progenitors from fibroblasts
JP5909482B2 (en) 2010-03-31 2016-04-26 ザ スクリプス リサーチ インスティテュート Cell reprogramming
JO3421B1 (en) 2011-06-20 2019-10-20 H Lundbeck As Method of administration of 4-((1R,3S)-6-chloro-3-phenyl-indan-1-yl)-1,2,2-trimethyl-piperazine and the salts thereof in the treatment of schizophrenia
US9166832B1 (en) 2013-10-04 2015-10-20 Altera Corporation Methods and apparatus for decision feedback equalization adaptation

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