BRPI0517973B8 - métodos para purificar o fsh recombinante - Google Patents

métodos para purificar o fsh recombinante

Info

Publication number
BRPI0517973B8
BRPI0517973B8 BRPI0517973A BRPI0517973A BRPI0517973B8 BR PI0517973 B8 BRPI0517973 B8 BR PI0517973B8 BR PI0517973 A BRPI0517973 A BR PI0517973A BR PI0517973 A BRPI0517973 A BR PI0517973A BR PI0517973 B8 BRPI0517973 B8 BR PI0517973B8
Authority
BR
Brazil
Prior art keywords
methods
fsh
recombinant fsh
purify recombinant
chromatography
Prior art date
Application number
BRPI0517973A
Other languages
English (en)
Inventor
Stanley Anne
Capponi Luciano
Delegrance Lydia
Valax Pascal
Wenger Pierre
Original Assignee
Ares Trading Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ares Trading Sa filed Critical Ares Trading Sa
Publication of BRPI0517973A publication Critical patent/BRPI0517973A/pt
Publication of BRPI0517973B1 publication Critical patent/BRPI0517973B1/pt
Publication of BRPI0517973B8 publication Critical patent/BRPI0517973B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/22—Hormones
    • A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Endocrinology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Reproductive Health (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pregnancy & Childbirth (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Fluid-Pressure Circuits (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Fats And Perfumes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Manufacturing Of Electric Cables (AREA)
  • Detergent Compositions (AREA)

Abstract

método para purificar o fsh. a presente invenção refere-se a um método para purificar o fsh recombinante humano ou uma variante de fsh, partindo do fsh bruto, compreendendo as seguintes etapas: 1.cromatografia de afinidade por corante; 2.cromatografia de interação hidrofóbica; e 3.cromatografla de fase reversa.
BRPI0517973A 2004-11-09 2005-11-08 métodos para purificar o fsh recombinante BRPI0517973B8 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP04105639.1 2004-11-09
EP04105639 2004-11-09
US62871704P 2004-11-17 2004-11-17
US60/628,717 2004-11-17
PCT/EP2005/055815 WO2006051070A1 (en) 2004-11-09 2005-11-08 Method for purifying fsh

Publications (3)

Publication Number Publication Date
BRPI0517973A BRPI0517973A (pt) 2008-10-21
BRPI0517973B1 BRPI0517973B1 (pt) 2018-02-14
BRPI0517973B8 true BRPI0517973B8 (pt) 2021-05-25

Family

ID=39560952

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0517973A BRPI0517973B8 (pt) 2004-11-09 2005-11-08 métodos para purificar o fsh recombinante

Country Status (22)

Country Link
US (1) US7741455B2 (pt)
EP (1) EP1809663B1 (pt)
KR (1) KR101173717B1 (pt)
CN (1) CN101087805B (pt)
AT (1) ATE408624T1 (pt)
AU (1) AU2005303818B2 (pt)
BR (1) BRPI0517973B8 (pt)
CA (1) CA2579131A1 (pt)
CY (1) CY1108602T1 (pt)
DE (1) DE602005009856D1 (pt)
DK (1) DK1809663T3 (pt)
EA (1) EA011673B1 (pt)
ES (1) ES2312037T3 (pt)
HK (1) HK1108704A1 (pt)
HR (1) HRP20080464T3 (pt)
IL (1) IL182716A (pt)
MX (1) MX2007005327A (pt)
NO (1) NO340811B1 (pt)
PL (1) PL1809663T3 (pt)
PT (1) PT1809663E (pt)
SI (1) SI1809663T1 (pt)
WO (1) WO2006051070A1 (pt)

Families Citing this family (29)

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EA008924B1 (ru) * 2003-12-22 2007-08-31 Арес Трейдинг С.А. Способ очистки фсг
LT1917276T (lt) * 2005-08-26 2018-05-25 Ares Trading S.A. Glikozilinto interferono beta gavimo būdas
WO2007065918A2 (en) * 2005-12-09 2007-06-14 Ares Trading S.A. Method for purifying fsh or a fsh mutant
WO2008150488A1 (en) * 2007-06-01 2008-12-11 Laboratory Corporation Of America Holdings Methods and systems for quantification of peptides and other analytes
AU2008267138B9 (en) 2007-06-28 2014-06-05 Theramex HQ UK Limited FSH producing cell clone
US20100249381A1 (en) * 2007-10-22 2010-09-30 David Delvaille Method for Purifying FC-Fusion Proteins
AU2008314689A1 (en) * 2007-10-22 2009-04-30 Merck Serono S.A. Method for purifying an Fc-containing protein
WO2009098318A1 (en) 2008-02-08 2009-08-13 Biogenerix Ag Liquid formulation of fsh
JP2009273427A (ja) * 2008-05-16 2009-11-26 Jcr Pharmaceuticals Co Ltd 組換え体ヒトｆｓｈの製造方法
JP5845170B2 (ja) 2009-04-01 2016-01-20 ラティオファーム ゲーエムベーハー 組換えｆｓｈを精製する方法
CN101519445B (zh) * 2009-04-08 2013-03-27 上海天伟生物制药有限公司 一种高比活尿促卵泡刺激素及其制备方法
CN101555279B (zh) * 2009-05-19 2013-03-27 上海天伟生物制药有限公司 一种高纯度尿促卵泡刺激素及其制备方法
CN101928342B (zh) * 2009-06-18 2013-05-08 上海天伟生物制药有限公司 一种高纯度绝经期促性腺素及其制备方法和用途
EP2325194A1 (en) 2009-11-24 2011-05-25 Glycotope GmbH Process for the purification of glycoproteins
JP2011172602A (ja) * 2011-06-15 2011-09-08 Jcr Pharmaceuticals Co Ltd 組換え体ヒトｆｓｈの製造方法
CN103570820B (zh) * 2012-08-06 2015-11-18 齐鲁制药有限公司 一种重组人促卵泡激素的纯化方法
US20160024181A1 (en) 2013-03-13 2016-01-28 Moderna Therapeutics, Inc. Long-lived polynucleotide molecules
JP5750471B2 (ja) * 2013-05-22 2015-07-22 Ｊｃｒファーマ株式会社 組換え体ヒトｆｓｈの製造方法
CN103539861B (zh) * 2013-11-01 2015-02-18 广州联康生物科技有限公司 长效重组人促卵泡激素融合蛋白
CN103539860B (zh) * 2013-11-01 2014-12-03 广州优联康医药科技有限公司 一种长效重组人促卵泡激素融合蛋白
CN103554269B (zh) * 2013-11-01 2015-02-11 广州联康生物科技有限公司 重组猪促卵泡激素融合蛋白
SG11201608513WA (en) 2014-04-18 2016-11-29 Cadila Healthcare Ltd Novel purification process of gonadotropin
CN104961821A (zh) * 2014-07-28 2015-10-07 厦门欧瑞捷生物科技有限公司 一种从人尿中提取促性腺激素的方法
WO2016116966A1 (en) * 2015-01-22 2016-07-28 Jcr Pharmaceuticals Co., Ltd. Method for purification of recombinant human alpha-galactosidase a from material containing contaminant host cell proteins
CN105461799A (zh) * 2015-12-31 2016-04-06 哈药集团技术中心 一种重组人促卵泡生成素的纯化方法
CN107698676B (zh) * 2017-11-07 2020-07-14 江西浩然生物医药有限公司 一种高纯度尿促性素的提取制备方法
CN110563832A (zh) * 2019-07-31 2019-12-13 江苏璟泽生物医药有限公司 一种高纯度重组促卵泡刺激素纯化方法
CN110305903B (zh) * 2019-07-31 2021-10-01 江苏璟泽生物医药有限公司 重组人促卵泡激素及其制备方法
CN113372433B (zh) * 2021-03-12 2022-10-11 上海景泽生物技术有限公司 纯化fsh方法

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IT1206302B (it) 1987-06-26 1989-04-14 Serono Cesare Ist Ricerca Ormone follicolo-stimolante urinario
US5665868A (en) 1990-09-14 1997-09-09 Vittal Mallya Scientific Research Foundation Chromatographic agent and its use for the separation or proteins, polypeptides of metals
SE9201073D0 (sv) 1992-04-03 1992-04-03 Kabi Pharmacia Ab Protein formulation
AU720337B2 (en) * 1995-02-17 2000-05-25 Stichting Dienst Landbouwkundig Onderzoek Production of biologically active recombinant bovine follicle stimulating hormone (REC bFSH) in the baculovirus expression system
AUPN801296A0 (en) 1996-02-12 1996-03-07 Csl Limited Stabilised growth hormone formulation and method of preparation thereof
IT1287138B1 (it) 1996-11-07 1998-08-04 Ibsa Inst Biochimique Sa Procedimento per la separazione e purificazione di fsh e lh
US6162905A (en) 1996-11-07 2000-12-19 Ibsa Institut Biochimique S.A. FSH and LH separation and purification process
US6414123B1 (en) * 1997-10-21 2002-07-02 Vitro Diagnostics, Inc. Method for purifying FSH
US5990288A (en) 1997-10-21 1999-11-23 Vitro Diagnostics, Inc. Method for purifying FSH
US20040010134A1 (en) * 2000-04-12 2004-01-15 Rosen Craig A. Albumin fusion proteins
DK1169349T3 (da) * 1999-04-16 2007-07-30 Inst Massone S A Proces til fremstilling af gonadotropinsammensætninger
KR100808149B1 (ko) 2000-02-22 2008-02-29 라보라토리스 세로노 에스.에이. 정제된 ｌｈ
AU784808B2 (en) * 2001-04-02 2006-06-29 Kedrion Melville Inc. Prion and viral clearance process
EA008924B1 (ru) * 2003-12-22 2007-08-31 Арес Трейдинг С.А. Способ очистки фсг
US7598356B2 (en) 2004-07-08 2009-10-06 Board of Regents of the University of Nebraska by and on behalf of the University of Nebraska Medical Center Method for purifying a protein of the cystine-knot superfamily

Also Published As

Publication number Publication date
DE602005009856D1 (de) 2008-10-30
WO2006051070A1 (en) 2006-05-18
EA200701040A1 (ru) 2007-10-26
IL182716A (en) 2010-12-30
BRPI0517973A (pt) 2008-10-21
EA011673B1 (ru) 2009-04-28
BRPI0517973B1 (pt) 2018-02-14
CA2579131A1 (en) 2006-05-18
KR101173717B1 (ko) 2012-08-13
IL182716A0 (en) 2007-07-24
ATE408624T1 (de) 2008-10-15
EP1809663A1 (en) 2007-07-25
CY1108602T1 (el) 2014-04-09
HK1108704A1 (en) 2008-05-16
DK1809663T3 (da) 2008-11-17
PT1809663E (pt) 2008-10-02
US7741455B2 (en) 2010-06-22
NO340811B1 (no) 2017-06-19
KR20070083618A (ko) 2007-08-24
MX2007005327A (es) 2007-08-02
SI1809663T1 (sl) 2009-02-28
US20080070832A1 (en) 2008-03-20
CN101087805B (zh) 2012-05-23
HRP20080464T3 (en) 2008-10-31
PL1809663T3 (pl) 2009-01-30
EP1809663B1 (en) 2008-09-17
AU2005303818B2 (en) 2011-06-23
ES2312037T3 (es) 2009-02-16
CN101087805A (zh) 2007-12-12
AU2005303818A1 (en) 2006-05-18
NO20072863L (no) 2007-06-05

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06H Technical and formal requirements: requirement cancelled [chapter 6.8 patent gazette]

Free format text: REF. A RPI NO 2153 DE 10/04/2012. ANULACAO DA EXIGENCIA 6.6 POR TER SIDO INDEVIDA. REQUERENTE NAO APRESENTOU RESPOSTA A EXIGENCIA CONFORME DISPOE A RESOLUCAO 207 DE 24/04/2009, ENTRETANTO, A RESPOSTA CONSTA NO FORMULARIO DE DEPOSITO.

B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]

Free format text: NOTIFICACAO DE ANUENCIA RELACIONADA COM O ART 229 DA LPI

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]
B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 08/11/2005 OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF