BRPI0309796B1 - processo para utilização de zymomonas mobilis geneticamente modificada, e zymomonas modificada - Google Patents
processo para utilização de zymomonas mobilis geneticamente modificada, e zymomonas modificada Download PDFInfo
- Publication number
- BRPI0309796B1 BRPI0309796B1 BRPI0309796A BR0309796A BRPI0309796B1 BR PI0309796 B1 BRPI0309796 B1 BR PI0309796B1 BR PI0309796 A BRPI0309796 A BR PI0309796A BR 0309796 A BR0309796 A BR 0309796A BR PI0309796 B1 BRPI0309796 B1 BR PI0309796B1
- Authority
- BR
- Brazil
- Prior art keywords
- xylose
- plasmid
- mobilis
- zymomonas
- strains
- Prior art date
Links
- 241000588901 Zymomonas Species 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 53
- 230000008569 process Effects 0.000 title claims abstract description 25
- 241000588902 Zymomonas mobilis Species 0.000 title claims description 109
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 206
- 238000000855 fermentation Methods 0.000 claims abstract description 86
- 230000004151 fermentation Effects 0.000 claims abstract description 86
- 235000000346 sugar Nutrition 0.000 claims abstract description 42
- 150000002972 pentoses Chemical class 0.000 claims abstract description 32
- 102000004190 Enzymes Human genes 0.000 claims abstract description 26
- 108090000790 Enzymes Proteins 0.000 claims abstract description 26
- 108020004530 Transaldolase Proteins 0.000 claims abstract description 25
- 102100028601 Transaldolase Human genes 0.000 claims abstract description 25
- 108700040099 Xylose isomerases Proteins 0.000 claims abstract description 23
- 102100029089 Xylulose kinase Human genes 0.000 claims abstract description 22
- 108091022915 xylulokinase Proteins 0.000 claims abstract description 22
- 239000000203 mixture Substances 0.000 claims description 10
- 230000017105 transposition Effects 0.000 abstract description 39
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 abstract description 28
- 238000002744 homologous recombination Methods 0.000 abstract description 24
- 230000006801 homologous recombination Effects 0.000 abstract description 24
- 238000004519 manufacturing process Methods 0.000 abstract description 24
- 230000000694 effects Effects 0.000 abstract description 20
- 230000002255 enzymatic effect Effects 0.000 abstract description 14
- -1 transquetolase Proteins 0.000 abstract description 7
- 239000011942 biocatalyst Substances 0.000 abstract description 3
- 230000005764 inhibitory process Effects 0.000 abstract description 3
- 108091026890 Coding region Proteins 0.000 abstract 1
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 293
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 212
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 206
- 239000013612 plasmid Substances 0.000 description 185
- 108090000623 proteins and genes Proteins 0.000 description 91
- 108020004414 DNA Proteins 0.000 description 58
- 239000012634 fragment Substances 0.000 description 54
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 48
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 45
- 239000008103 glucose Substances 0.000 description 45
- 230000010354 integration Effects 0.000 description 44
- 241000588724 Escherichia coli Species 0.000 description 40
- 210000004027 cell Anatomy 0.000 description 35
- 230000012010 growth Effects 0.000 description 35
- 239000000523 sample Substances 0.000 description 34
- 238000012546 transfer Methods 0.000 description 34
- 239000002609 medium Substances 0.000 description 32
- 238000003752 polymerase chain reaction Methods 0.000 description 29
- 101100446506 Mus musculus Fgf3 gene Proteins 0.000 description 28
- 101150106193 tal gene Proteins 0.000 description 25
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 24
- 108010020764 Transposases Proteins 0.000 description 22
- 238000003780 insertion Methods 0.000 description 22
- 230000037431 insertion Effects 0.000 description 22
- 238000004458 analytical method Methods 0.000 description 20
- 230000021615 conjugation Effects 0.000 description 20
- 101150024271 TKT gene Proteins 0.000 description 19
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 18
- SRBFZHDQGSBBOR-HWQSCIPKSA-N L-arabinopyranose Chemical compound O[C@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-HWQSCIPKSA-N 0.000 description 18
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 16
- 239000013615 primer Substances 0.000 description 16
- 239000002994 raw material Substances 0.000 description 16
- 239000013598 vector Substances 0.000 description 15
- 102000008579 Transposases Human genes 0.000 description 14
- 238000010367 cloning Methods 0.000 description 14
- 230000014509 gene expression Effects 0.000 description 14
- 101100537319 Escherichia coli (strain K12) tktA gene Proteins 0.000 description 13
- 101100170068 Mus musculus Ddr2 gene Proteins 0.000 description 13
- 230000003115 biocidal effect Effects 0.000 description 13
- 238000010276 construction Methods 0.000 description 13
- 101150110790 xylB gene Proteins 0.000 description 13
- 238000002105 Southern blotting Methods 0.000 description 12
- 101150104734 ldh gene Proteins 0.000 description 12
- 239000000047 product Substances 0.000 description 12
- 101100454398 Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) ldh1 gene Proteins 0.000 description 11
- 230000006978 adaptation Effects 0.000 description 11
- 101100157012 Thermoanaerobacterium saccharolyticum (strain DSM 8691 / JW/SL-YS485) xynB gene Proteins 0.000 description 10
- 238000004520 electroporation Methods 0.000 description 10
- 239000003550 marker Substances 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 9
- 239000002028 Biomass Substances 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- 238000004128 high performance liquid chromatography Methods 0.000 description 8
- 238000009396 hybridization Methods 0.000 description 8
- 239000006152 selective media Substances 0.000 description 8
- 238000003556 assay Methods 0.000 description 7
- 230000029087 digestion Effects 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 7
- 230000009466 transformation Effects 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 108010018080 L-arabinose isomerase Proteins 0.000 description 6
- 239000004098 Tetracycline Substances 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000011987 methylation Effects 0.000 description 6
- 238000007069 methylation reaction Methods 0.000 description 6
- 230000010076 replication Effects 0.000 description 6
- 108020002667 ribulokinase Proteins 0.000 description 6
- 229960002180 tetracycline Drugs 0.000 description 6
- 229930101283 tetracycline Natural products 0.000 description 6
- 235000019364 tetracycline Nutrition 0.000 description 6
- 150000003522 tetracyclines Chemical class 0.000 description 6
- 101150014795 tktA gene Proteins 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 238000013459 approach Methods 0.000 description 5
- 239000006227 byproduct Substances 0.000 description 5
- 239000001913 cellulose Substances 0.000 description 5
- 229920002678 cellulose Polymers 0.000 description 5
- 230000002950 deficient Effects 0.000 description 5
- 238000007824 enzymatic assay Methods 0.000 description 5
- 230000002068 genetic effect Effects 0.000 description 5
- 239000002054 inoculum Substances 0.000 description 5
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 5
- 238000002703 mutagenesis Methods 0.000 description 5
- 231100000350 mutagenesis Toxicity 0.000 description 5
- MHWLWQUZZRMNGJ-UHFFFAOYSA-N nalidixic acid Chemical compound C1=C(C)N=C2N(CC)C=C(C(O)=O)C(=O)C2=C1 MHWLWQUZZRMNGJ-UHFFFAOYSA-N 0.000 description 5
- 229960000210 nalidixic acid Drugs 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- AEMOLEFTQBMNLQ-VANFPWTGSA-N D-mannopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H]1O AEMOLEFTQBMNLQ-VANFPWTGSA-N 0.000 description 4
- 101710136501 Mannan endo-1,4-beta-mannosidase Proteins 0.000 description 4
- 101150049837 PGM gene Proteins 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 239000000446 fuel Substances 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000006798 recombination Effects 0.000 description 4
- 238000005215 recombination Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 150000008163 sugars Chemical class 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 101100121991 Chlamydia pneumoniae glmM gene Proteins 0.000 description 3
- 101100536311 Drosophila melanogaster Taldo gene Proteins 0.000 description 3
- 101100480526 Drosophila melanogaster tal-1A gene Proteins 0.000 description 3
- 101100205917 Drosophila melanogaster tal-2A gene Proteins 0.000 description 3
- 101100312937 Drosophila melanogaster tal-3A gene Proteins 0.000 description 3
- 101100312939 Drosophila melanogaster tal-AA gene Proteins 0.000 description 3
- 108090000416 L-ribulose-5-phosphate 4-epimerases Proteins 0.000 description 3
- 101100098715 Mus musculus Taldo1 gene Proteins 0.000 description 3
- 101150038013 PIR gene Proteins 0.000 description 3
- 108020004511 Recombinant DNA Proteins 0.000 description 3
- 101100205913 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) tal1 gene Proteins 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 230000025938 carbohydrate utilization Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 101150084612 gpmA gene Proteins 0.000 description 3
- 101150104722 gpmI gene Proteins 0.000 description 3
- 230000035876 healing Effects 0.000 description 3
- 229930027917 kanamycin Natural products 0.000 description 3
- 229960000318 kanamycin Drugs 0.000 description 3
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
- 229930182823 kanamycin A Natural products 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 239000013600 plasmid vector Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 230000010473 stable expression Effects 0.000 description 3
- UJOBWOGCFQCDNV-UHFFFAOYSA-N 9H-carbazole Chemical compound C1=CC=C2C3=CC=CC=C3NC2=C1 UJOBWOGCFQCDNV-UHFFFAOYSA-N 0.000 description 2
- 108020001019 DNA Primers Proteins 0.000 description 2
- 239000003155 DNA primer Substances 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- VZUNGTLZRAYYDE-UHFFFAOYSA-N N-methyl-N'-nitro-N-nitrosoguanidine Chemical compound O=NN(C)C(=N)N[N+]([O-])=O VZUNGTLZRAYYDE-UHFFFAOYSA-N 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 108010002747 Pfu DNA polymerase Proteins 0.000 description 2
- 108010011939 Pyruvate Decarboxylase Proteins 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 238000000376 autoradiography Methods 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 108010030074 endodeoxyribonuclease MluI Proteins 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000001320 lysogenic effect Effects 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 101150037893 mppe-1 gene Proteins 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000004108 pentose phosphate pathway Effects 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000024540 transposon integration Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 101150011516 xlnD gene Proteins 0.000 description 2
- 101150052264 xylA gene Proteins 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- CFKMVGJGLGKFKI-UHFFFAOYSA-N 4-chloro-m-cresol Chemical compound CC1=CC(O)=CC=C1Cl CFKMVGJGLGKFKI-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 108010072454 CTGCAG-specific type II deoxyribonucleases Proteins 0.000 description 1
- 101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241001112695 Clostridiales Species 0.000 description 1
- 206010010144 Completed suicide Diseases 0.000 description 1
- 108010051219 Cre recombinase Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 1
- 102100029211 E3 ubiquitin-protein ligase TTC3 Human genes 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 101000851432 Escherichia coli Transposase for transposon Tn5 Proteins 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 229910003556 H2 SO4 Inorganic materials 0.000 description 1
- 101000633723 Homo sapiens E3 ubiquitin-protein ligase TTC3 Proteins 0.000 description 1
- 108090000769 Isomerases Proteins 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 101100348848 Mus musculus Notch4 gene Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 101150034686 PDC gene Proteins 0.000 description 1
- 241001520808 Panicum virgatum Species 0.000 description 1
- 101710150114 Protein rep Proteins 0.000 description 1
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 1
- 108700005075 Regulator Genes Proteins 0.000 description 1
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 1
- 101710152114 Replication protein Proteins 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 108010018858 Tn10 transposase Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002962 chemical mutagen Substances 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 1
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 210000003712 lysosome Anatomy 0.000 description 1
- 230000001868 lysosomic effect Effects 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000009343 monoculture Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 229940098006 multigen Drugs 0.000 description 1
- 238000007857 nested PCR Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000002205 phenol-chloroform extraction Methods 0.000 description 1
- 238000013492 plasmid preparation Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 101150040618 talB gene Proteins 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
- C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/134,655 US7223575B2 (en) | 2000-05-01 | 2002-04-27 | Zymomonas pentose-sugar fermenting strains and uses thereof |
| PCT/US2003/012726 WO2004037973A2 (en) | 2002-04-27 | 2003-04-25 | Zymomonas pentose-sugar fermentating strains and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR0309796A BR0309796A (pt) | 2005-08-16 |
| BRPI0309796B1 true BRPI0309796B1 (pt) | 2016-07-12 |
Family
ID=32174134
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0309796A BRPI0309796B1 (pt) | 2002-04-27 | 2003-04-25 | processo para utilização de zymomonas mobilis geneticamente modificada, e zymomonas modificada |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7223575B2 (enExample) |
| EP (1) | EP1549736B1 (enExample) |
| JP (1) | JP4309845B2 (enExample) |
| CN (1) | CN1665919A (enExample) |
| BR (1) | BRPI0309796B1 (enExample) |
| CA (1) | CA2483574A1 (enExample) |
| DE (1) | DE60327230D1 (enExample) |
| DK (1) | DK1549736T3 (enExample) |
| MX (1) | MXPA04010651A (enExample) |
| WO (1) | WO2004037973A2 (enExample) |
Families Citing this family (113)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070029252A1 (en) * | 2005-04-12 | 2007-02-08 | Dunson James B Jr | System and process for biomass treatment |
| US7781191B2 (en) * | 2005-04-12 | 2010-08-24 | E. I. Du Pont De Nemours And Company | Treatment of biomass to obtain a target chemical |
| JP5149785B2 (ja) * | 2005-04-12 | 2013-02-20 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | エタノールを得るためのバイオマスの処理 |
| US7659104B2 (en) | 2006-05-05 | 2010-02-09 | E.I. Du Pont De Nemours And Company | Solvent tolerant microorganisms and methods of isolation |
| US7541173B2 (en) | 2006-06-15 | 2009-06-02 | E.I. Du Pont De Nemours And Company | Solvent tolerant microorganisms and methods of isolation |
| MY157916A (en) | 2006-07-21 | 2016-08-15 | Xyleco Inc | Conversion system for biomass |
| US7741119B2 (en) * | 2006-09-28 | 2010-06-22 | E. I. Du Pont De Nemours And Company | Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production |
| US7741084B2 (en) | 2006-09-28 | 2010-06-22 | E. I. Du Pont De Nemours And Company | Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas |
| US7629156B2 (en) | 2006-09-28 | 2009-12-08 | E.I. Du Pont De Nemours And Company | Ethanol production in fermentation of mixed sugars containing xylose |
| US7807419B2 (en) * | 2007-08-22 | 2010-10-05 | E. I. Du Pont De Nemours And Company | Process for concentrated biomass saccharification |
| US20090053800A1 (en) * | 2007-08-22 | 2009-02-26 | Julie Friend | Biomass Treatment Apparatus |
| US8445236B2 (en) * | 2007-08-22 | 2013-05-21 | Alliance For Sustainable Energy Llc | Biomass pretreatment |
| US7819976B2 (en) * | 2007-08-22 | 2010-10-26 | E. I. Du Pont De Nemours And Company | Biomass treatment method |
| JP4124270B1 (ja) * | 2007-10-05 | 2008-07-23 | 国立大学法人鳥取大学 | グルコース・マンノース・キシロース並行発酵性菌およびそれを用いるバイオエタノールの製造方法 |
| ATE506447T1 (de) | 2007-10-30 | 2011-05-15 | Du Pont | Verfahren zur herstellung von etahnol in einem xylose enthaltenden medium unter verwendung eines rekombinanten zymomonas stammes mit reduzierter hima expression |
| BRPI0817151A8 (pt) * | 2007-10-30 | 2018-12-11 | Du Pont | micro-organismo recombinante e processo para gerar o micro-organismo |
| JPWO2009081941A1 (ja) * | 2007-12-25 | 2011-05-06 | 国立大学法人鳥取大学 | 木質系および草本系バイオマス糖化液からのバイオエタノール並行発酵菌 |
| JP4427583B2 (ja) | 2008-02-01 | 2010-03-10 | 三菱重工業株式会社 | バイオマスの水熱分解装置及び方法、バイオマス原料を用いた有機原料の製造システム |
| JP4524351B2 (ja) | 2008-02-01 | 2010-08-18 | 三菱重工業株式会社 | バイオマス原料を用いた有機原料の製造システム及び方法 |
| JP4427584B2 (ja) | 2008-02-01 | 2010-03-10 | 三菱重工業株式会社 | バイオマスの水熱分解装置及び方法、バイオマス原料を用いた有機原料の製造システム |
| CA2716585C (en) * | 2008-03-27 | 2017-05-09 | E.I. Du Pont De Nemours And Company | Zymomonas recombinant for substituted glyceraldehyde-3-phosphate dehydrogenase promoter and xylose isomerase gene for improved xylose utilization |
| ES2627671T3 (es) * | 2008-03-27 | 2017-07-31 | E. I. Du Pont De Nemours And Company | Promotores de Zymomonas de alta expresión |
| US8329444B2 (en) * | 2008-04-23 | 2012-12-11 | Georgia Tech Research Corporation | Strains of zymomonas mobilis for fermentation of biomass |
| US20100159521A1 (en) * | 2008-12-19 | 2010-06-24 | E. I. Du Pont De Nemours And Company | Ozone treatment of biomass to enhance enzymatic saccharification |
| US8247208B2 (en) | 2008-12-22 | 2012-08-21 | Alliance For Sustainable Energy Llc | Zymomonas with improved xylose utilization in stress conditions |
| WO2010147642A1 (en) | 2009-06-15 | 2010-12-23 | Massachusetts Institute Of Technology | Production of triacylglycerides, fatty acids, and their derivatives |
| US20110143408A1 (en) * | 2009-06-18 | 2011-06-16 | E. I. Du Pont De Nemours And Company | Zymomonas with improved arabinose utilization |
| WO2010151574A2 (en) | 2009-06-25 | 2010-12-29 | Shell Oil Company | Water injection systems and methods |
| US8575471B2 (en) * | 2009-08-31 | 2013-11-05 | Alliance For Sustainable Energy, Llc | Lattice matched semiconductor growth on crystalline metallic substrates |
| US8961687B2 (en) * | 2009-08-31 | 2015-02-24 | Alliance For Sustainable Energy, Llc | Lattice matched crystalline substrates for cubic nitride semiconductor growth |
| US8785159B2 (en) | 2009-11-25 | 2014-07-22 | Alliance For Sustainable Energy, Llc | Extracellular secretion of recombinant proteins |
| WO2011065539A1 (ja) * | 2009-11-30 | 2011-06-03 | 国立大学法人神戸大学 | バイオマスからのエタノールの生産方法 |
| US8507365B2 (en) * | 2009-12-21 | 2013-08-13 | Alliance For Sustainable Energy, Llc | Growth of coincident site lattice matched semiconductor layers and devices on crystalline substrates |
| CN107287250A (zh) | 2009-12-23 | 2017-10-24 | 丹尼斯科美国公司 | 提高同步糖化发酵反应效率的方法 |
| US8647850B2 (en) | 2009-12-23 | 2014-02-11 | E I Du Pont De Nemours And Company | Process for simultaneous saccharification and fermentation for production of ethanol |
| US8721794B2 (en) | 2010-04-28 | 2014-05-13 | E I Du Pont De Nemours And Company | Production of high solids syrup from lignocellulosic biomass hydrolysate fermentation broth |
| US8906235B2 (en) | 2010-04-28 | 2014-12-09 | E I Du Pont De Nemours And Company | Process for liquid/solid separation of lignocellulosic biomass hydrolysate fermentation broth |
| CN102947456A (zh) | 2010-06-18 | 2013-02-27 | 布特马斯先进生物燃料有限责任公司 | 补充脂肪酸以改善醇产量 |
| US9040263B2 (en) | 2010-07-28 | 2015-05-26 | Butamax Advanced Biofuels Llc | Production of alcohol esters and in situ product removal during alcohol fermentation |
| AU2011268220B2 (en) | 2010-06-18 | 2015-01-29 | Butamax(Tm) Advanced Biofuels Llc | Extraction solvents derived from oil for alcohol removal in extractive fermentation |
| US8679822B2 (en) | 2010-06-29 | 2014-03-25 | E I Du Pont De Nemours And Company | Xylose utilization in recombinant zymomonas |
| US8623623B2 (en) | 2010-06-29 | 2014-01-07 | E I Du Pont De Nemours And Company | Xylose utilization in recombinant Zymomonas |
| US8651403B2 (en) | 2010-07-21 | 2014-02-18 | E I Du Pont De Nemours And Company | Anhydrous ammonia treatment for improved milling of biomass |
| CN102399804A (zh) * | 2010-09-15 | 2012-04-04 | 中国农业科学院作物科学研究所 | 木糖异构酶基因的功能及其应用 |
| WO2012047832A2 (en) | 2010-10-07 | 2012-04-12 | Shell Oil Company | Process for the production of alcohols from biomass |
| CN103189526B (zh) | 2010-11-05 | 2015-07-08 | 国际壳牌研究有限公司 | 处理生物质以产生可用于生物燃料的材料 |
| US20120125551A1 (en) | 2010-11-23 | 2012-05-24 | E. I. Du Pont De Nemours And Company | Biomass pretreatment process for a packed bed reactor |
| US20120125548A1 (en) | 2010-11-23 | 2012-05-24 | E. I. Du Pont De Nemours And Company | Continuously fed biomass pretreatment process for a packed bed reactor |
| WO2012074523A1 (en) | 2010-12-01 | 2012-06-07 | Alliance For Sustainable Energy, Llc | Methods of producing free-standing semiconductors using sacrificial buffer layers and recyclable substrates |
| US9425249B2 (en) | 2010-12-01 | 2016-08-23 | Alliance For Sustainable Energy, Llc | Coincident site lattice-matched growth of semiconductors on substrates using compliant buffer layers |
| US8476048B2 (en) | 2010-12-17 | 2013-07-02 | E I Du Pont De Nemours And Company | Xylose utilizing zymomonas mobilis with improved ethanol production in biomass hydrolysate medium |
| WO2012088108A1 (en) | 2010-12-20 | 2012-06-28 | Shell Oil Company | Process for the production of alcohols from biomass |
| US20120196342A1 (en) | 2011-02-01 | 2012-08-02 | Rachel Ruizhen Chen | Industrial Applications of A Novel Aldo/Keto Reductase Of Zymomonas Mobilis |
| US20130040340A1 (en) | 2011-02-07 | 2013-02-14 | E. I. Du Pont De Nemours And Company | Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms |
| US8759044B2 (en) | 2011-03-23 | 2014-06-24 | Butamax Advanced Biofuels Llc | In situ expression of lipase for enzymatic production of alcohol esters during fermentation |
| US8765425B2 (en) | 2011-03-23 | 2014-07-01 | Butamax Advanced Biofuels Llc | In situ expression of lipase for enzymatic production of alcohol esters during fermentation |
| US8765426B2 (en) | 2011-04-07 | 2014-07-01 | E I Du Pont De Nemours And Company | Pantothenic acid biosynthesis in zymomonas |
| MX2013014327A (es) | 2011-06-17 | 2014-01-23 | Butamax Tm Advanced Biofuels | Coproductos de procesos de produccion de biocombustible y metodos para elaborarlos. |
| CA2838519A1 (en) | 2011-06-17 | 2012-12-20 | Butamax Advanced Biofuels Llc | Lignocellulosic hydrolysates as feedstocks for isobutanol fermentation |
| EP2785853A2 (en) | 2011-12-02 | 2014-10-08 | BP Corporation North America Inc. | Compositions and methods for biomass liquefaction |
| WO2013086222A2 (en) | 2011-12-09 | 2013-06-13 | Butamax(Tm) Advanced Biofuels Llc | Process to remove product alcohols from fermentation broth |
| PL2791344T3 (pl) | 2011-12-15 | 2016-08-31 | Shell Int Research | Sposób obróbki produktów ubocznych z wytwarzania etanolu |
| US8911983B2 (en) | 2011-12-20 | 2014-12-16 | E I Du Pont De Nemours And Company | Pnp gene modification for improved xylose utilization in Zymomonas |
| US20130157332A1 (en) | 2011-12-20 | 2013-06-20 | E I Du Pont De Nemours And Company | Gene inactivation allowing immediate growth on xylose medium by engineered zymomonas |
| CN104254613A (zh) | 2012-02-13 | 2014-12-31 | Bp北美公司 | 木质纤维素水解产物脱毒的方法 |
| US9133278B2 (en) | 2012-02-13 | 2015-09-15 | Bp Corporation North America Inc. | Methods for detoxifying a lignocellulosic hydrolysate |
| JP2015517303A (ja) | 2012-05-04 | 2015-06-22 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | アルコールの製造および回収のための方法およびシステム |
| CN104395478B (zh) | 2012-05-07 | 2017-07-28 | 国际壳牌研究有限公司 | 处理生物质以生产可用于生物燃料的材料的连续或半连续方法 |
| CA2873309A1 (en) | 2012-05-17 | 2013-11-21 | Phillip Guy Hamilton | Methods and systems for processing biomass material |
| US20140024064A1 (en) | 2012-07-23 | 2014-01-23 | Butamax(Tm) Advanced Biofuels Llc | Processes and systems for the production of fermentative alcohols |
| DK2844731T3 (en) | 2012-08-06 | 2018-05-07 | Danisco Us Inc | MUSHROOM CELLS WHICH MAKE REDUCED QUANTITIES OF PEPTAIBOLS |
| EP2895612A1 (en) | 2012-09-12 | 2015-07-22 | Butamax Advanced Biofuels LLC | Processes and systems for the production of fermentation products |
| US9249076B2 (en) | 2012-09-21 | 2016-02-02 | Butamax Advanced Biofuels Llc | Recyclable extractant compositions |
| AU2013329024A1 (en) | 2012-10-11 | 2015-03-12 | Butamax Advanced Biofuels Llc | Processes and systems for the production of fermentation products |
| WO2014081848A1 (en) | 2012-11-20 | 2014-05-30 | Butamax Advanced Biofuels Llc | Butanol purification |
| BR112015013933A2 (pt) | 2012-12-14 | 2017-07-11 | Bp Corp North America Inc | fermentação sequencial de hidrolisato e sólidos de uma hidrólise de ácido diluído de biomassa para produzir produtos de fermentação |
| US8846357B2 (en) | 2012-12-20 | 2014-09-30 | E I Du Pont De Nemours And Company | Stabilized chlorine dioxide for contamination control in Zymomonas fermentation |
| WO2014130352A1 (en) | 2013-02-21 | 2014-08-28 | Butamax Advanced Biofuels Llc | Recovery of thermal energy as heat source in the production of butanol from a fermentation process |
| WO2014159309A1 (en) | 2013-03-12 | 2014-10-02 | Butamax Advanced Biofuels Llc | Processes and systems for the production of alcohols |
| US20140273105A1 (en) | 2013-03-12 | 2014-09-18 | E I Du Pont De Nemours And Company | Gradient pretreatment of lignocellulosic biomass |
| WO2014160050A1 (en) | 2013-03-14 | 2014-10-02 | Butamax Advanced Biofuels Llc | Glycerol 3- phosphate dehydrogenase for butanol production |
| WO2014160262A1 (en) | 2013-03-14 | 2014-10-02 | Abengoa Bioenergy New Technologies, Llc | Methods for converting cellulosic waste to bioproducts |
| CA2901374A1 (en) | 2013-03-15 | 2014-09-18 | Butamax Advanced Biofuels Llc | Method for production of butanol using extractive fermentation |
| WO2014151645A1 (en) | 2013-03-15 | 2014-09-25 | Butamax Advanced Biofuels Llc | Process for maximizing biomass growth and butanol yield by feedback control |
| WO2014145768A2 (en) | 2013-03-15 | 2014-09-18 | Bp Corporation North America Inc. | Use of non-fungal 5' utrs in filamentous fungi |
| WO2014144643A1 (en) | 2013-03-15 | 2014-09-18 | Butamax Advanced Biofuels Llc | Method for producing butanol using extractive fermentation |
| DE102013205986A1 (de) * | 2013-04-04 | 2014-10-23 | Wacker Chemie Ag | Mikroorganismenstamm und Verfahren zur fermentativen Herstellung von C4-Verbindungen aus C5-Zuckern |
| US9206445B2 (en) | 2013-04-29 | 2015-12-08 | Alliance For Sustainable Energy, Llc | Biocatalysts with enhanced inhibitor tolerance |
| WO2015002913A1 (en) | 2013-07-03 | 2015-01-08 | Butamax Advanced Biofuels Llc | Partial adaptation for butanol production |
| WO2015031561A1 (en) | 2013-08-30 | 2015-03-05 | Bp Corporation North America Inc. | Catalytic conversion of alcohols |
| US20150087040A1 (en) | 2013-09-26 | 2015-03-26 | E I Du Pont De Nemours And Company | Production of ethanol and recycle water in a cellulosic fermentation process |
| US20150087041A1 (en) | 2013-09-26 | 2015-03-26 | E I Du Pont De Nemours And Company | Production of ethanol with reduced contaminants in a cellulosic biomass based process |
| US9139818B2 (en) | 2013-09-26 | 2015-09-22 | E I Du Pont De Nemours And Company | High expression Zymomonas promoters |
| WO2015057517A1 (en) | 2013-10-17 | 2015-04-23 | Danisco Us Inc. | Use of hemicellulases to improve ethanol production |
| US10227623B2 (en) | 2013-11-24 | 2019-03-12 | E I Du Pont De Nemours And Company | High force and high stress destructuring of cellulosic biomass |
| US20150197757A1 (en) | 2014-01-16 | 2015-07-16 | Wisconsin Alumni Research Foundation | Oxygen-responsive bacterial gene switch |
| WO2015160887A1 (en) | 2014-04-17 | 2015-10-22 | Shell Oil Company | Processes for producing fermentation products |
| US9371546B2 (en) | 2014-06-24 | 2016-06-21 | E I Du Pont De Nemours And Company | Enhancing D-xylose and L-arabinose utilization in Zymomonas cells |
| WO2016007350A1 (en) | 2014-07-09 | 2016-01-14 | Danisco Us Inc. | Preconditioning of lignocellulosic biomass |
| CN106536496A (zh) | 2014-08-14 | 2017-03-22 | 国际壳牌研究有限公司 | 从生物质制备糠醛的方法 |
| WO2016025678A1 (en) | 2014-08-14 | 2016-02-18 | Shell Oil Company | Process for preparing furfural from biomass |
| BR112017002204B1 (pt) | 2014-08-14 | 2021-01-12 | Shell Internationale Research Maatschappij B.V. | métodos para recuperar ácido a-hidroxisulfônico em um processo de tratamento de biomassa |
| JP6977231B2 (ja) | 2015-07-13 | 2021-12-08 | マラ リニューアブルズ コーポレーション | C5有機炭素の微生物による代謝の増強 |
| US20180230446A1 (en) | 2015-07-29 | 2018-08-16 | Abengoa Bioenergía Nuevas Tecnologías, S.A. | Expression of recombinant beta-xylosidase enzymes |
| WO2017116840A1 (en) | 2015-12-28 | 2017-07-06 | Danisco Us Inc. | Methods for the production of fermentation products from feedstock |
| US10479773B2 (en) | 2016-05-03 | 2019-11-19 | Shell Oil Company | Lignin-based solvents and methods for their preparation |
| US10899725B2 (en) | 2016-11-01 | 2021-01-26 | Shell Oil Company | Process for the recovery of furfural |
| EP3535248A1 (en) | 2016-11-01 | 2019-09-11 | Shell Internationale Research Maatschappij B.V. | Process for the recovery of furfural |
| CA3039603A1 (en) | 2016-11-01 | 2018-05-11 | Shell Internationale Research Maatschappij B.V. | Process for the recovery of furfural |
| WO2018085182A1 (en) | 2016-11-01 | 2018-05-11 | Shell Oil Company | Process for the recovery of furfural |
| US11787773B2 (en) | 2019-05-22 | 2023-10-17 | Shell Usa, Inc. | Process for the production of furfural |
| CN113444763B (zh) * | 2021-08-16 | 2022-11-08 | 农业部沼气科学研究所 | 树干毕赤酵母与运动发酵单胞菌混菌发酵葡萄糖和木糖产乙醇的方法 |
| AU2024212235A1 (en) | 2023-01-23 | 2025-07-03 | Shell Internationale Research Maatschappij B.V. | Method for treating grains to produce material useful for chemicals and biofuels |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5514583A (en) * | 1994-04-15 | 1996-05-07 | Midwest Research Institute | Recombinant zymomonas for pentose fermentation |
| US5843760A (en) * | 1994-04-15 | 1998-12-01 | Midwest Research Institute | Single zymomonas mobilis strain for xylose and arabinose fermentation |
| US5712133A (en) * | 1994-04-15 | 1998-01-27 | Midwest Research Institute | Pentose fermentation by recombinant zymomonas |
| JP4510355B2 (ja) * | 2000-05-01 | 2010-07-21 | ミッドウエスト リサーチ インスティチュート | 安定なチモモナスモビリスのキシロースおよびアラビノース醗酵株 |
| US7374939B1 (en) * | 2000-05-01 | 2008-05-20 | Midwest Research Institute | Method of inactivation of an end product of energy metabolism in Zymomonas mobilis |
| US7354755B2 (en) * | 2000-05-01 | 2008-04-08 | Midwest Research Institute | Stable zymomonas mobilis xylose and arabinose fermenting strains |
| US6566107B1 (en) * | 2000-11-01 | 2003-05-20 | Midwest Research Institute | Recombinant Zymomonas mobilis with improved xylose utilization |
-
2002
- 2002-04-27 US US10/134,655 patent/US7223575B2/en not_active Expired - Fee Related
-
2003
- 2003-04-25 DE DE60327230T patent/DE60327230D1/de not_active Expired - Lifetime
- 2003-04-25 CA CA002483574A patent/CA2483574A1/en not_active Abandoned
- 2003-04-25 WO PCT/US2003/012726 patent/WO2004037973A2/en not_active Ceased
- 2003-04-25 CN CN038152290A patent/CN1665919A/zh active Pending
- 2003-04-25 JP JP2004546658A patent/JP4309845B2/ja not_active Expired - Fee Related
- 2003-04-25 MX MXPA04010651A patent/MXPA04010651A/es not_active Application Discontinuation
- 2003-04-25 BR BRPI0309796A patent/BRPI0309796B1/pt not_active IP Right Cessation
- 2003-04-25 EP EP03799754A patent/EP1549736B1/en not_active Expired - Lifetime
- 2003-04-25 DK DK03799754T patent/DK1549736T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| EP1549736A4 (en) | 2006-12-13 |
| JP4309845B2 (ja) | 2009-08-05 |
| BR0309796A (pt) | 2005-08-16 |
| EP1549736B1 (en) | 2009-04-15 |
| CA2483574A1 (en) | 2004-05-06 |
| DK1549736T3 (da) | 2009-07-20 |
| WO2004037973A2 (en) | 2004-05-06 |
| US20030162271A1 (en) | 2003-08-28 |
| JP2005534343A (ja) | 2005-11-17 |
| CN1665919A (zh) | 2005-09-07 |
| EP1549736A2 (en) | 2005-07-06 |
| MXPA04010651A (es) | 2006-02-24 |
| DE60327230D1 (de) | 2009-05-28 |
| US7223575B2 (en) | 2007-05-29 |
| WO2004037973A3 (en) | 2004-09-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BRPI0309796B1 (pt) | processo para utilização de zymomonas mobilis geneticamente modificada, e zymomonas modificada | |
| CN102037120B (zh) | 具有改善的木糖利用率的发酵单胞菌属 | |
| US7354755B2 (en) | Stable zymomonas mobilis xylose and arabinose fermenting strains | |
| JP5444323B2 (ja) | 高発現ザイモモナス(Zymomonas)プロモーター | |
| JP5907865B2 (ja) | 改善されたアラビノース資化能を有するザイモモナス(zymomonas) | |
| BRPI0618074A2 (pt) | organismos termofìlicos para conversão de biomassa lignocelulósica em etanol | |
| AU2001249926B2 (en) | Stable zymomonas mobilis xylose and arabinose fermenting strains | |
| AU2001249926A1 (en) | Stable zymomonas mobilis xylose and arabinose fermenting strains | |
| EP1366178B1 (en) | Method of site-specific insertion in zymomonas mobilis | |
| AU2001251397A1 (en) | Method of site-specific insertion in zymomonas mobilis | |
| CN101974547B (zh) | 含FLP的pBBR1MCS-2重组质粒及运动发酵单胞菌基因组DNA修饰的方法 | |
| AU2003299464B8 (en) | Zymomonas pentose-sugar fermentating strains and uses thereof | |
| CA2304929C (en) | Stable zymomonas mobilis xylose and arabinose fermenting strains | |
| Zhang et al. | Stable Zymomonas mobilis xylose and arabinose fermenting strains |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06G | Technical and formal requirements: other requirements [chapter 6.7 patent gazette] |
Free format text: APRESENTE O DEPOSITANTE TRADUCAO COMPLETA DO PEDIDO, CONFORME DETERMINA O ATO NORMATIVO N 128 DE 19970305, ITENS 9.2 E 9.2.1 |
|
| B06I | Publication of requirement cancelled [chapter 6.9 patent gazette] | ||
| B25A | Requested transfer of rights approved |
Owner name: ALLIANCE FOR SUSTAINABLE ENERGY, LLC (US) Free format text: TRANSFERIDO DE: MIDWEST RESEARCH INSTITUTE |
|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 12/07/2016, OBSERVADAS AS CONDICOES LEGAIS. |
|
| B21F | Lapse acc. art. 78, item iv - on non-payment of the annual fees in time |
Free format text: REFERENTE A 20A ANUIDADE. |
|
| B24J | Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12) |
Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2720 DE 23-02-2023 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |