BR0309796A - Linhagens de zymomonas de fermentação de açúcar pentose e usos das mesmas, processo, método, integrante de zymomonas e biocatalisador - Google Patents

Linhagens de zymomonas de fermentação de açúcar pentose e usos das mesmas, processo, método, integrante de zymomonas e biocatalisador

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Publication number
BR0309796A
BR0309796A BR0309796-0A BR0309796A BR0309796A BR 0309796 A BR0309796 A BR 0309796A BR 0309796 A BR0309796 A BR 0309796A BR 0309796 A BR0309796 A BR 0309796A
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Brazil
Prior art keywords
zymomonas
pentose
members
fermentation
integrant
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BR0309796-0A
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English (en)
Other versions
BRPI0309796B1 (pt
Inventor
Min Zhang
Yat-Chen
William Howe
Christine Eddy
Kent Evans
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Midwest Research Inst
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Publication date
Application filed by Midwest Research Inst filed Critical Midwest Research Inst
Publication of BR0309796A publication Critical patent/BR0309796A/pt
Publication of BRPI0309796B1 publication Critical patent/BRPI0309796B1/pt

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    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"LINHAGENS DE ZYMOMONAS DE FERMENTAçãO DE AçúCAR PENTOSE E USOS DAS MESMAS, PROCESSO, MéTODO, INTEGRANTE DE ZYMOMONAS E BIOCATALISADOR". Um integrante de Zymomonas tem a capacidade de fermentar a pentose em etanol. O operon que codifica as enzimas de fermentação da pentose são integradas no genoma de Zymomonas em um evento de duas integrações de recombinação homóloga e transposição. Cada operon pode incluir mais que uma seq³ência de codificação de enzima redutora da pentose. Em algumas configurações, o integrante inclui seq³ências enzimáticas que codificam a xilose isomerase, xiluloquinase, transquetolase e transaldolase. Os integrantes de Zymomonas são altamente estáveis e mantêm atividade para a produção da enzima de fermentação da pentose entre 80 a 160 gerações. Os integrantes também são resistentes à inibição do acetato, uma vez que os integrantes demonstram uma eficiente produção de etanol mesmo na presença de 8 até 16 gramas de acetato por litro de meio.
BRPI0309796A 2002-04-27 2003-04-25 processo para utilização de zymomonas mobilis geneticamente modificada, e zymomonas modificada BRPI0309796B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/134,655 US7223575B2 (en) 2000-05-01 2002-04-27 Zymomonas pentose-sugar fermenting strains and uses thereof
PCT/US2003/012726 WO2004037973A2 (en) 2002-04-27 2003-04-25 Zymomonas pentose-sugar fermentating strains and uses thereof

Publications (2)

Publication Number Publication Date
BR0309796A true BR0309796A (pt) 2005-08-16
BRPI0309796B1 BRPI0309796B1 (pt) 2016-07-12

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BRPI0309796A BRPI0309796B1 (pt) 2002-04-27 2003-04-25 processo para utilização de zymomonas mobilis geneticamente modificada, e zymomonas modificada

Country Status (10)

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US (1) US7223575B2 (pt)
EP (1) EP1549736B1 (pt)
JP (1) JP4309845B2 (pt)
CN (1) CN1665919A (pt)
BR (1) BRPI0309796B1 (pt)
CA (1) CA2483574A1 (pt)
DE (1) DE60327230D1 (pt)
DK (1) DK1549736T3 (pt)
MX (1) MXPA04010651A (pt)
WO (1) WO2004037973A2 (pt)

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