BR9910532A - Produção melhorada in vivo de cefalosporina - Google Patents

Produção melhorada in vivo de cefalosporina

Info

Publication number
BR9910532A
BR9910532A BR9910532-2A BR9910532A BR9910532A BR 9910532 A BR9910532 A BR 9910532A BR 9910532 A BR9910532 A BR 9910532A BR 9910532 A BR9910532 A BR 9910532A
Authority
BR
Brazil
Prior art keywords
improved
vivo
host cell
host cells
localised
Prior art date
Application number
BR9910532-2A
Other languages
English (en)
Inventor
Maarten Nieboer
Roelof Ary Lans Bovenberg
Original Assignee
Dsm Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Nv filed Critical Dsm Nv
Publication of BR9910532A publication Critical patent/BR9910532A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cephalosporin Compounds (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

"PRODUçãO MELHORADA IN VIVO DE CEFALOSPORINA" A invenção apresenta DNA, células hospedeiras heterólogas, capazes de transcrever, traduzir, ou expressar o referido DNA e processos empregando tais células hospedeiras e suas culturas para uma produção melhorada in vivo de cefalosporinas aciladas com rendimento mais elevado. De acordo com a invenção, é apresentada uma célula hospedeira, que compreende uma enzima que possui atividade de expandase, que está predominante localizada no citossol (ao contrário de estar localizada principalmente em, ou com os peroxissomos ou microcorpos) da célula hospedeira.
BR9910532-2A 1998-05-19 1999-05-19 Produção melhorada in vivo de cefalosporina BR9910532A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98201655 1998-05-19
PCT/EP1999/003455 WO1999060102A2 (en) 1998-05-19 1999-05-19 Improved in vivo production of cephalosporins

Publications (1)

Publication Number Publication Date
BR9910532A true BR9910532A (pt) 2001-10-16

Family

ID=8233737

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9910532-2A BR9910532A (pt) 1998-05-19 1999-05-19 Produção melhorada in vivo de cefalosporina

Country Status (16)

Country Link
US (2) US6995003B1 (pt)
EP (2) EP1953230A1 (pt)
JP (1) JP2003527069A (pt)
KR (1) KR100648480B1 (pt)
CN (2) CN101353660A (pt)
AT (1) ATE386123T1 (pt)
AU (1) AU4144999A (pt)
BR (1) BR9910532A (pt)
DE (1) DE69938129D1 (pt)
ES (1) ES2302379T3 (pt)
ID (1) ID27054A (pt)
MX (1) MXPA00011223A (pt)
PL (1) PL344863A1 (pt)
RU (1) RU2230790C2 (pt)
WO (1) WO1999060102A2 (pt)
ZA (1) ZA200006308B (pt)

Families Citing this family (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0011185D0 (en) * 2000-05-09 2000-06-28 Synpac Pharmaceuticals Limited Protein
CN1796537B (zh) * 2004-12-23 2010-09-08 华北制药集团有限责任公司 一种新的产黄青霉菌株以及利用此菌株发酵制备青霉素
US20060217971A1 (en) * 2005-03-28 2006-09-28 Tellabs Operations, Inc. Method and apparatus for modifying an encoded signal
CA2704654C (en) 2007-11-20 2015-02-10 Dsm Ip Assets B.V. Succinic acid production in a eukaryotic cell
ES2389989T3 (es) 2007-11-20 2012-11-05 Dsm Ip Assets B.V. Producción de ácido dicarboxílico en levadura recombinante
US20110318795A1 (en) 2007-11-20 2011-12-29 Verwaal Rene Dicarboxylic acid production in a filamentous fungus
EP2116136A1 (en) 2008-05-08 2009-11-11 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Novel phytases
US8735100B2 (en) 2009-03-17 2014-05-27 Nederlandse Organisatie Voor Toegepast -Natuurwetenschappelijk Onderzoek Tno Cellulose and ligno-cellulose active proteins
WO2010118932A1 (en) 2009-04-15 2010-10-21 Dsm Ip Assets B.V. Dicarboxylic acid production process
UA108853C2 (uk) 2009-07-10 2015-06-25 Спосіб ферментації галактози
MX2012012171A (es) 2010-04-21 2013-02-27 Dsm Ip Assets Bv Proceso para la produccion de celulas que tienen la capacidad de convertir arabinosa.
EP2388331A1 (en) 2010-05-21 2011-11-23 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Constitutive promoter
CN103189505A (zh) 2010-10-13 2013-07-03 帝斯曼知识产权资产管理有限公司 具有通透酶活性的多肽
WO2012143513A2 (en) 2011-04-22 2012-10-26 Dsm Ip Assets B.V. Yeast cell capable of converting sugars including arabinose and xylose
EP2554668A1 (en) 2011-08-04 2013-02-06 DSM IP Assets B.V. A pentose sugar fermenting cell
AR087423A1 (es) 2011-08-04 2014-03-19 Dsm Ip Assets Bv Celula capaz de fermentar azucares pentosas
CN104203005A (zh) 2012-01-23 2014-12-10 帝斯曼知识产权资产管理有限公司 二萜的生产
BR112015008370B1 (pt) 2012-10-16 2022-10-18 Dsm Ip Assets B.V Células de saccharomyces com conversão de pentose melhorada e processo para a produção de um produto da fermentação
EP2772545A1 (en) 2013-03-01 2014-09-03 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Starch active proteins
EP2981610A1 (en) 2013-04-05 2016-02-10 Université du Luxembourg Biotechnological production of itaconic acid
WO2014178717A1 (en) 2013-05-02 2014-11-06 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Novel organic acid pathway
EP3004366B1 (en) 2013-05-31 2019-02-27 DSM IP Assets B.V. Microorganisms for diterpene production
EP3885443A1 (en) 2013-07-15 2021-09-29 DSM IP Assets B.V. Diterpene production
CN108064226A (zh) 2013-07-31 2018-05-22 帝斯曼知识产权资产管理有限公司 甜菊糖苷的回收
CN107429273A (zh) 2015-02-16 2017-12-01 帝斯曼知识产权资产管理有限公司 用于在厌氧条件下生产衣康酸的方法
EP3862426A3 (en) 2015-03-16 2021-11-17 DSM IP Assets B.V. Udp-glycosyltransferases
EP3274448B1 (en) 2015-03-23 2021-08-11 DSM IP Assets B.V. Udp-glycosyltransferases from solanum lycopersicum
EP3718417A1 (en) 2015-04-03 2020-10-07 DSM IP Assets B.V. Steviol glycosides
WO2016170045A1 (en) 2015-04-21 2016-10-27 Dsm Ip Assets B.V. Geranylgeranyl pyrophosphate synthase
ES2755680T3 (es) 2015-05-22 2020-04-23 Dsm Ip Assets Bv Célula de levadura que consume acetato
MY192153A (en) 2015-07-10 2022-08-02 Dsm Ip Assets Bv Steviol glycoside composition
WO2017025649A1 (en) 2015-08-13 2017-02-16 Dsm Ip Assets B.V. Steviol glycoside transport
WO2017050652A1 (en) 2015-09-25 2017-03-30 Dsm Ip Assets B.V. Asparaginase
BR112018006724A2 (pt) 2015-10-05 2018-10-09 Dsm Ip Assets Bv ácido caurenoico hidroxilases
MY186400A (en) 2015-10-06 2021-07-22 Dsm Ip Assets Bv Eukaryotic cell with increased production of fermentation product
KR20180097745A (ko) 2015-12-30 2018-08-31 디에스엠 아이피 어셋츠 비.브이. 트라이아실글리세롤의 부분 효소적 가수 분해
CN116064263A (zh) 2015-12-30 2023-05-05 帝斯曼知识产权资产管理有限公司 三酰基甘油的部分酶水解
EP3435771A1 (en) 2016-03-31 2019-02-06 DSM IP Assets B.V. Enzyme composition and preparation of a dairy product with improved properties
WO2017167849A1 (en) 2016-03-31 2017-10-05 Dsm Ip Assets B.V. Enzyme composition and preparation of a dairy product with improved properties
EP3435770A1 (en) 2016-03-31 2019-02-06 DSM IP Assets B.V. Production of milk with low lactose content
WO2017216136A1 (en) 2016-06-14 2017-12-21 Dsm Ip Assets B.V. Recombinant yeast cell
CN116286420A (zh) 2016-07-13 2023-06-23 帝斯曼知识产权资产管理有限公司 苹果酸脱氢酶
CA3033243A1 (en) 2016-08-09 2018-02-15 Dsm Ip Assets B.V. Crystallization of steviol glycosides
EP3497109A1 (en) 2016-08-09 2019-06-19 DSM IP Assets B.V. Crystallization of steviol glycosides
CN110036114B (zh) 2016-08-26 2023-09-29 勒萨弗尔公司 提高的衣康酸生产
WO2018073107A1 (en) 2016-10-19 2018-04-26 Dsm Ip Assets B.V. Eukaryotic cell comprising xylose isomerase
US11225647B2 (en) 2016-10-27 2022-01-18 Dsm Ip Assets B.V. Geranylgeranyl pyrophosphate synthases
BR112019011525A2 (pt) 2016-12-08 2020-01-28 Dsm Ip Assets Bv ácido caurenoico hidroxilases
EP3559222A1 (en) 2016-12-21 2019-10-30 DSM IP Assets B.V. Lipolytic enzyme variants
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
US10889807B2 (en) 2016-12-21 2021-01-12 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114995A1 (en) 2016-12-22 2018-06-28 Dsm Ip Assets B.V. Fermentation process for producing steviol glycosides
CA3064143A1 (en) 2017-06-13 2018-12-20 Dsm Ip Assets B.V. Recombinant yeast cell
EP4155400A1 (en) 2017-06-27 2023-03-29 DSM IP Assets B.V. Udp-glycosyltransferases
CN111133111A (zh) 2017-09-26 2020-05-08 帝斯曼知识产权资产管理有限公司 消耗乙酸的菌株
EP3533878A1 (en) 2018-02-28 2019-09-04 Dutch DNA Biotech B.V. Process for producing citramalic acid employing aspergillus
BR112020025589A2 (pt) 2018-06-19 2021-03-23 Dsm Ip Assets B.V. variantes de enzima lipolítica
AU2021367159A1 (en) 2020-10-22 2023-05-04 Cargill, Incorporated Microorganisms for diterpene production
AU2022297881A1 (en) 2021-06-24 2024-01-18 Fonterra Co-Operative Group Limited Recombinant beta-lactoglobulin
EP4370651A1 (en) 2021-07-12 2024-05-22 Danisco Us Inc Recombinant yeast cell
WO2023285294A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
CN118056011A (zh) 2021-07-12 2024-05-17 丹尼斯科美国公司 重组酵母细胞
WO2023285280A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
EP4370688A1 (en) 2021-07-12 2024-05-22 Danisco Us Inc Recombinant yeast cell
EP4370691A1 (en) 2021-07-12 2024-05-22 Danisco US Inc. Recombinant yeast cell
WO2023079049A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Variant polypeptide and recombinant yeast cell
WO2023079050A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Recombinant yeast cell
WO2023079048A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Process for the production of ethanol and recombinant yeast cell
AU2022388727A1 (en) 2021-11-09 2024-05-16 Amgen Inc. Production of therapeutic proteins
WO2023222614A1 (en) 2022-05-16 2023-11-23 Dsm Ip Assets B.V. Lipolytic enzyme variants

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4003894A (en) 1971-08-17 1977-01-18 Gist-Brocades N.V. Preparation of 7-substituted amino-desacetoxycephalosporanic acid compounds
PT83746B (pt) 1985-11-15 1988-08-17 Gist Brocades Nv Processo para a preparacao de novos biocatalisadores imobilizados e para a producao de etanol por fermentacao
US5070020A (en) 1988-05-09 1991-12-03 Eli Lilly And Company Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase
US5082772A (en) 1988-10-24 1992-01-21 Eli Lilly And Company Process for preparing deacetylcephalosporin c
PT97116B (pt) * 1990-03-23 1998-10-30 Gist Brocades Nv Processo para modular a producao de metabolitos secundarios nomeadamente derivados de beta-lactama
US5318896A (en) 1991-09-11 1994-06-07 Merck & Co., Inc. Recombinant expandase bioprocess for preparing 7-aminodesacetoxy cephalosporanic acid (7-ADCA)
EP0635574B1 (en) 1993-07-23 2003-04-23 Dsm N.V. Selection marker gene free recombinant strains, a method for obtaining them and the use of these strains
SK282617B6 (sk) * 1993-07-30 2002-10-08 Dsm N. V. Spôsob výroby a izolácie 7-aminodesacetoxycefalosporánovej kyseliny
PL180799B1 (pl) 1993-07-30 2001-04-30 Gist Brocades Bv Sposób wydajnego wytwarzania 7-ADCA poprzez 2-(karboksyetylotio)acetylo-7-ADCA i 3-(karboksymetylotio)-propionylo-7-ADCA
EP0863989A2 (en) * 1995-11-27 1998-09-16 Gist-Brocades B.V. Improved process for the production of semi-synthetic cephalosporins via expandase activity on penicillin g
AU4114697A (en) 1996-07-16 1998-02-09 Gist-Brocades B.V. Improved process for the production of adipoyl cephalosporins

Also Published As

Publication number Publication date
WO1999060102A2 (en) 1999-11-25
ATE386123T1 (de) 2008-03-15
ZA200006308B (en) 2001-05-23
CN100390285C (zh) 2008-05-28
ES2302379T3 (es) 2008-07-01
DE69938129D1 (de) 2008-03-27
US20060127972A1 (en) 2006-06-15
KR100648480B1 (ko) 2006-11-24
RU2230790C2 (ru) 2004-06-20
AU4144999A (en) 1999-12-06
MXPA00011223A (es) 2002-04-17
CN101353660A (zh) 2009-01-28
CN1301303A (zh) 2001-06-27
KR20010025039A (ko) 2001-03-26
JP2003527069A (ja) 2003-09-16
EP1078069A2 (en) 2001-02-28
PL344863A1 (en) 2001-11-19
WO1999060102A3 (en) 2000-02-03
US6995003B1 (en) 2006-02-07
EP1078069B1 (en) 2008-02-13
ID27054A (id) 2001-02-22
EP1953230A1 (en) 2008-08-06

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