BR9706059A - Dna recombinante autonomamente replicável em células de bactérias corineformes, bactéria corineforme abrigando uma aspartoquinase, processo para produzir l-lisa, dna, e vetor pvk7 - Google Patents

Dna recombinante autonomamente replicável em células de bactérias corineformes, bactéria corineforme abrigando uma aspartoquinase, processo para produzir l-lisa, dna, e vetor pvk7

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Publication number
BR9706059A
BR9706059A BR9706059-3A BR9706059A BR9706059A BR 9706059 A BR9706059 A BR 9706059A BR 9706059 A BR9706059 A BR 9706059A BR 9706059 A BR9706059 A BR 9706059A
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Brazil
Prior art keywords
aspartokinase
dna
dna sequence
lysine
bacteria
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Application number
BR9706059-3A
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English (en)
Other versions
BR9706059B1 (pt
Inventor
Masayuki Araki
Masakazu Sugimoto
Yasuhiki Yoshihara
Tsuyoshi Nakamatsu
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Ajinomoto Kk
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=18179293&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR9706059(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Publication of BR9706059A publication Critical patent/BR9706059A/pt
Publication of BR9706059B1 publication Critical patent/BR9706059B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"DNA RECOMBINANTE AUTONOMAMENTE REPLICáVEL EM CéLULAS DE BACTéRIAS CORINEFORMES, BACTéRIA CORINEFORME ABRIGANDO UMA ASPARTOQUINASE, PROCESSO PARA PRODUZIR L-LISA, DNA, E, VETOR PVK7". Uma bactéria corineforme abrigando uma aspartoquinase em que a inibição de retro-alimentação por L-lisina e L-treonina é substancialmente dessensibilizada, e compreendendo uma sequência de DNA melhorada codificando para reductase di-hidrodipicolinato, uma sequência de DNA melhorada codificando para reductase di-hidropicolinato, uma sequência de DNA melhorada codificando para sintase di-hidropicolinato, uma sequência de DNA melhorada codificando para descarboxilase diaminopimelato, e uma sequência DNA melhorada codificando para aminotransferase aspartato, um processo para produzir L-lisina compreendendo as etapas de cultivar a bactéria corineforme em um meio apropriado para permitir que L-lisina seja produzida e acumulada em uma cultura da bactéria, e coletar L-lisina da cultura; e um DNA recombinante utilizável para produção de bactéria corineforme.
BRPI9706059-3A 1996-12-05 1997-12-05 vetor recombinante, bactéria corineforme transgência, e, processo para produzir l-lisina. BR9706059B1 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP32565996 1996-12-05

Publications (2)

Publication Number Publication Date
BR9706059A true BR9706059A (pt) 1999-10-05
BR9706059B1 BR9706059B1 (pt) 2012-02-22

Family

ID=18179293

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI9706059-3A BR9706059B1 (pt) 1996-12-05 1997-12-05 vetor recombinante, bactéria corineforme transgência, e, processo para produzir l-lisina.

Country Status (10)

Country Link
US (1) US6004773A (pt)
EP (1) EP0854189B1 (pt)
CN (2) CN1159443C (pt)
BR (1) BR9706059B1 (pt)
DE (1) DE69736698T2 (pt)
DK (1) DK0854189T3 (pt)
ES (1) ES2273355T3 (pt)
HU (1) HU224492B1 (pt)
PL (1) PL190430B1 (pt)
SK (1) SK285201B6 (pt)

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JP4075087B2 (ja) 1996-12-05 2008-04-16 味の素株式会社 Ｌ−リジンの製造法
DE19931317A1 (de) * 1999-07-07 2001-01-11 Degussa L-Lysin produzierende coryneforme Bakterien und Verfahren zur Herstellung von L-Lysin
JP2003135066A (ja) * 1999-03-19 2003-05-13 Ajinomoto Co Inc Ｌ−リジンの製造法
KR100878334B1 (ko) * 1999-06-25 2009-01-14 백광산업 주식회사 대사 경로 단백질을 코딩하는 코리네박테리움 글루타미쿰유전자
BR0012020A (pt) 1999-07-02 2002-07-02 Ajinomoto Kk Proteìna, e, dna
JP2003169674A (ja) * 1999-07-02 2003-06-17 Ajinomoto Co Inc Ｌ−リジンの製造法
JP2003159092A (ja) * 1999-07-02 2003-06-03 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
US20020086371A1 (en) 1999-07-07 2002-07-04 Degussa-Huls Aktiengesellschaft L-lysine-producing corynebacteria and process for the preparation of L-lysine
US6861246B2 (en) 1999-07-07 2005-03-01 Degussa Ag L-lysine-producing corynebacteria and process for the preparation of lysine
DE19931314A1 (de) * 1999-07-07 2001-01-11 Degussa L-Lysin produzierende coryneforme Bakterien und Verfahren zur Herstellung von Lysin
DE19943587A1 (de) 1999-09-11 2001-03-15 Degussa Neue für das dapF-Gen codierende Nukleotidsequenzen
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US20040121428A1 (en) * 1999-12-24 2004-06-24 Masakazu Sugimoto Process for producing l-amino acid and novel gene
US6927046B1 (en) * 1999-12-30 2005-08-09 Archer-Daniels-Midland Company Increased lysine production by gene amplification using coryneform bacteria
WO2002022666A2 (en) * 2000-09-12 2002-03-21 Degussa Ag Nucleotide sequences which code for the gora gene
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KR20090023513A (ko) 2004-10-07 2009-03-04 아지노모토 가부시키가이샤 염기성 물질의 제조법
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DE102006032634A1 (de) 2006-07-13 2008-01-17 Evonik Degussa Gmbh Verfahren zur Herstellung von L-Aminosäuren
EP1881076A1 (en) * 2006-07-17 2008-01-23 Evonik Degussa GmbH Method for producing L-amino acids
WO2008033001A1 (en) * 2006-09-15 2008-03-20 Cj Cheiljedang Corporation A corynebacteria having enhanced l-lysine productivity and a method of producing l-lysine using the same
KR100830826B1 (ko) 2007-01-24 2008-05-19 씨제이제일제당 (주) 코리네박테리아를 이용하여 글리세롤을 포함한탄소원으로부터 발효산물을 생산하는 방법
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CN114540399B (zh) * 2022-02-15 2024-05-03 宁夏伊品生物科技股份有限公司 一种制备l-缬氨酸的方法及其所用基因突变体和生物材料
CN114835783B (zh) * 2022-06-01 2024-06-25 宁夏伊品生物科技股份有限公司 NCgl2747基因突变体及其在制备L-赖氨酸中的应用

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Also Published As

Publication number Publication date
DE69736698T2 (de) 2007-09-20
BR9706059B1 (pt) 2012-02-22
US6004773A (en) 1999-12-21
DK0854189T3 (da) 2007-01-15
CN1159443C (zh) 2004-07-28
EP0854189B1 (en) 2006-09-20
PL190430B1 (pl) 2005-12-30
SK285201B6 (sk) 2006-08-03
HU9702360D0 (en) 1998-03-02
CN1524956A (zh) 2004-09-01
HUP9702360A3 (en) 2002-12-28
HU224492B1 (hu) 2005-09-28
ES2273355T3 (es) 2007-05-01
CN1273592C (zh) 2006-09-06
DE69736698D1 (de) 2006-11-02
EP0854189A3 (en) 2002-11-06
EP0854189A2 (en) 1998-07-22
SK163697A3 (en) 1998-07-08
CN1187540A (zh) 1998-07-15
PL323546A1 (en) 1998-06-08
HUP9702360A2 (hu) 1999-06-28

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Free format text: DE ACORDO COM O ART. 37, INDEFIRO DO PRESENTE PEDIDO, UMA VEZ QUE NAO APRESENTA SUFICIENCIA DESCRITIVA (ART. 24 DA LPI), AS REIVINDICACOES ESTAO INDEFINIDAS E/OU NAO ESTAO FUNDAMENTADAS NO RELATORIO DESCRITIVO (ART. 25 DA LPI) E ABRANGE MATERIA QUE NAO E CONSIDERADA INVENCAO, UMA VEZ QUE INCIDE NO ART. 10 DA LPI.

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