BR9706059A - Dna recombinante autonomamente replicável em células de bactérias corineformes, bactéria corineforme abrigando uma aspartoquinase, processo para produzir l-lisa, dna, e vetor pvk7 - Google Patents

Dna recombinante autonomamente replicável em células de bactérias corineformes, bactéria corineforme abrigando uma aspartoquinase, processo para produzir l-lisa, dna, e vetor pvk7

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Publication number
BR9706059A
BR9706059A BR9706059-3A BR9706059A BR9706059A BR 9706059 A BR9706059 A BR 9706059A BR 9706059 A BR9706059 A BR 9706059A BR 9706059 A BR9706059 A BR 9706059A
Authority
BR
Brazil
Prior art keywords
aspartokinase
dna
dna sequence
lysine
bacteria
Prior art date
Application number
BR9706059-3A
Other languages
English (en)
Other versions
BR9706059B1 (pt
Inventor
Masayuki Araki
Masakazu Sugimoto
Yasuhiki Yoshihara
Tsuyoshi Nakamatsu
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=18179293&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR9706059(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Publication of BR9706059A publication Critical patent/BR9706059A/pt
Publication of BR9706059B1 publication Critical patent/BR9706059B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine

Abstract

"DNA RECOMBINANTE AUTONOMAMENTE REPLICáVEL EM CéLULAS DE BACTéRIAS CORINEFORMES, BACTéRIA CORINEFORME ABRIGANDO UMA ASPARTOQUINASE, PROCESSO PARA PRODUZIR L-LISA, DNA, E, VETOR PVK7". Uma bactéria corineforme abrigando uma aspartoquinase em que a inibição de retro-alimentação por L-lisina e L-treonina é substancialmente dessensibilizada, e compreendendo uma sequência de DNA melhorada codificando para reductase di-hidrodipicolinato, uma sequência de DNA melhorada codificando para reductase di-hidropicolinato, uma sequência de DNA melhorada codificando para sintase di-hidropicolinato, uma sequência de DNA melhorada codificando para descarboxilase diaminopimelato, e uma sequência DNA melhorada codificando para aminotransferase aspartato, um processo para produzir L-lisina compreendendo as etapas de cultivar a bactéria corineforme em um meio apropriado para permitir que L-lisina seja produzida e acumulada em uma cultura da bactéria, e coletar L-lisina da cultura; e um DNA recombinante utilizável para produção de bactéria corineforme.
BRPI9706059-3A 1996-12-05 1997-12-05 vetor recombinante, bactéria corineforme transgência, e, processo para produzir l-lisina. BR9706059B1 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP32565996 1996-12-05

Publications (2)

Publication Number Publication Date
BR9706059A true BR9706059A (pt) 1999-10-05
BR9706059B1 BR9706059B1 (pt) 2012-02-22

Family

ID=18179293

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI9706059-3A BR9706059B1 (pt) 1996-12-05 1997-12-05 vetor recombinante, bactéria corineforme transgência, e, processo para produzir l-lisina.

Country Status (10)

Country Link
US (1) US6004773A (pt)
EP (1) EP0854189B1 (pt)
CN (2) CN1273592C (pt)
BR (1) BR9706059B1 (pt)
DE (1) DE69736698T2 (pt)
DK (1) DK0854189T3 (pt)
ES (1) ES2273355T3 (pt)
HU (1) HU224492B1 (pt)
PL (1) PL190430B1 (pt)
SK (1) SK285201B6 (pt)

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US20020086371A1 (en) 1999-07-07 2002-07-04 Degussa-Huls Aktiengesellschaft L-lysine-producing corynebacteria and process for the preparation of L-lysine
DE19943587A1 (de) * 1999-09-11 2001-03-15 Degussa Neue für das dapF-Gen codierende Nukleotidsequenzen
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CA2437656C (en) 2001-02-08 2009-06-30 Archer-Daniels-Midland Company Polynucleotide constructs for increased lysine production
DE10154292A1 (de) 2001-11-05 2003-05-15 Basf Ag Gene die für Stoffwechselweg-Proteine codieren
RU2244007C2 (ru) * 2002-02-27 2005-01-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Способ получения l-треонина, штамм escherichia coli - продуцент треонина (варианты)
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KR100830826B1 (ko) 2007-01-24 2008-05-19 씨제이제일제당 (주) 코리네박테리아를 이용하여 글리세롤을 포함한탄소원으로부터 발효산물을 생산하는 방법
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Also Published As

Publication number Publication date
CN1187540A (zh) 1998-07-15
CN1273592C (zh) 2006-09-06
CN1159443C (zh) 2004-07-28
HU224492B1 (hu) 2005-09-28
CN1524956A (zh) 2004-09-01
PL190430B1 (pl) 2005-12-30
EP0854189A2 (en) 1998-07-22
HUP9702360A3 (en) 2002-12-28
DK0854189T3 (da) 2007-01-15
EP0854189A3 (en) 2002-11-06
ES2273355T3 (es) 2007-05-01
BR9706059B1 (pt) 2012-02-22
HU9702360D0 (en) 1998-03-02
HUP9702360A2 (hu) 1999-06-28
PL323546A1 (en) 1998-06-08
DE69736698T2 (de) 2007-09-20
US6004773A (en) 1999-12-21
DE69736698D1 (de) 2006-11-02
SK285201B6 (sk) 2006-08-03
SK163697A3 (en) 1998-07-08
EP0854189B1 (en) 2006-09-20

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B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
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Free format text: DE ACORDO COM O ART. 37, INDEFIRO DO PRESENTE PEDIDO, UMA VEZ QUE NAO APRESENTA SUFICIENCIA DESCRITIVA (ART. 24 DA LPI), AS REIVINDICACOES ESTAO INDEFINIDAS E/OU NAO ESTAO FUNDAMENTADAS NO RELATORIO DESCRITIVO (ART. 25 DA LPI) E ABRANGE MATERIA QUE NAO E CONSIDERADA INVENCAO, UMA VEZ QUE INCIDE NO ART. 10 DA LPI.

B12B Appeal against refusal [chapter 12.2 patent gazette]
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