BR112018068934A2 - método de modificação de moléculas de ácido nucleico alvo (na alvo) em células, molécula, molécula de funa recombinante, vetor e seu uso, sistema de vetor, sistema de modificação de na alvo em células, composição - Google Patents
método de modificação de moléculas de ácido nucleico alvo (na alvo) em células, molécula, molécula de funa recombinante, vetor e seu uso, sistema de vetor, sistema de modificação de na alvo em células, composiçãoInfo
- Publication number
- BR112018068934A2 BR112018068934A2 BR112018068934A BR112018068934A BR112018068934A2 BR 112018068934 A2 BR112018068934 A2 BR 112018068934A2 BR 112018068934 A BR112018068934 A BR 112018068934A BR 112018068934 A BR112018068934 A BR 112018068934A BR 112018068934 A2 BR112018068934 A2 BR 112018068934A2
- Authority
- BR
- Brazil
- Prior art keywords
- molecule
- cells
- vector
- nucleic acid
- target nucleic
- Prior art date
Links
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 150000007523 nucleic acids Chemical class 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 238000012986 modification Methods 0.000 title 1
- 230000004048 modification Effects 0.000 title 1
- 230000004927 fusion Effects 0.000 abstract 2
- 108091033409 CRISPR Proteins 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 101710163270 Nuclease Proteins 0.000 abstract 1
- 230000006801 homologous recombination Effects 0.000 abstract 1
- 238000002744 homologous recombination Methods 0.000 abstract 1
- 238000002715 modification method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Epidemiology (AREA)
- Peptides Or Proteins (AREA)
- AIDS & HIV (AREA)
- Tropical Medicine & Parasitology (AREA)
- Ophthalmology & Optometry (AREA)
Abstract
métodos de modificação de ácidos nucleicos alvo. o método envolve uma construção na qual rna guia é ligado covalentemente a rna doador (na de fusão) a ser introduzido no ácido nucleico alvo por meio de recombinação homóloga e é baseado na introdução de nuclease, tal como crispr ou talen, na célula que contém o ácido nucleico alvo. o na de fusão pode ser introduzido na forma de vetor de dna.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP16167774 | 2016-04-29 | ||
EP16167773 | 2016-04-29 | ||
EP17156018 | 2017-02-14 | ||
PCT/EP2017/059331 WO2017186550A1 (en) | 2016-04-29 | 2017-04-20 | Improved methods for modification of target nucleic acids |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112018068934A2 true BR112018068934A2 (pt) | 2019-01-22 |
Family
ID=58640844
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112018068934A BR112018068934A2 (pt) | 2016-04-29 | 2017-04-20 | método de modificação de moléculas de ácido nucleico alvo (na alvo) em células, molécula, molécula de funa recombinante, vetor e seu uso, sistema de vetor, sistema de modificação de na alvo em células, composição |
Country Status (13)
Country | Link |
---|---|
US (5) | US11608499B2 (pt) |
EP (5) | EP4166660A1 (pt) |
JP (2) | JP7184648B2 (pt) |
KR (3) | KR102370675B1 (pt) |
CN (1) | CN109072207B (pt) |
AU (5) | AU2017256517B2 (pt) |
BR (1) | BR112018068934A2 (pt) |
CA (1) | CA3022475A1 (pt) |
CL (1) | CL2018003074A1 (pt) |
DK (1) | DK3448990T5 (pt) |
IL (1) | IL262409A (pt) |
MX (2) | MX2018013256A (pt) |
WO (1) | WO2017186550A1 (pt) |
Families Citing this family (47)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3613852A3 (en) | 2011-07-22 | 2020-04-22 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
US9068179B1 (en) | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
CA2956224A1 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
AU2015342749B2 (en) | 2014-11-07 | 2022-01-27 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
CA2999500A1 (en) | 2015-09-24 | 2017-03-30 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
AU2016342380B2 (en) | 2015-10-23 | 2022-04-07 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
EP4047092A1 (en) | 2016-04-13 | 2022-08-24 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
EA201892810A1 (ru) | 2016-06-01 | 2019-06-28 | Квс Заат Се | Гибридные последовательности нуклеиновых кислот для геномной инженерии |
GB2568182A (en) | 2016-08-03 | 2019-05-08 | Harvard College | Adenosine nucleobase editors and uses thereof |
JP7201153B2 (ja) | 2016-08-09 | 2023-01-10 | プレジデント アンド フェローズ オブ ハーバード カレッジ | プログラム可能cas9-リコンビナーゼ融合タンパク質およびその使用 |
US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
SG11201903089RA (en) | 2016-10-14 | 2019-05-30 | Harvard College | Aav delivery of nucleobase editors |
US20190330620A1 (en) * | 2016-10-14 | 2019-10-31 | Emendobio Inc. | Rna compositions for genome editing |
EP3541945A4 (en) * | 2016-11-18 | 2020-12-09 | Genedit Inc. | COMPOSITIONS AND METHODS OF MODIFICATION OF TARGET NUCLEIC ACIDS |
WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
BR112019015578A2 (pt) * | 2017-01-30 | 2020-03-10 | KWS SAAT SE & Co. KGaA | Ligação de modelo de reparo a endonucleases para engenharia de genoma |
US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
WO2018165629A1 (en) | 2017-03-10 | 2018-09-13 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
CA3057192A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
SG11201912179SA (en) | 2017-06-15 | 2020-01-30 | Univ California | Targeted non-viral dna insertions |
EP3652312A1 (en) | 2017-07-14 | 2020-05-20 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
CA3082251A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
WO2019084552A1 (en) | 2017-10-27 | 2019-05-02 | The Regents Of The University Of California | TARGETED REPLACEMENT OF ENDOGENIC T CELL RECEPTORS |
US11268092B2 (en) | 2018-01-12 | 2022-03-08 | GenEdit, Inc. | Structure-engineered guide RNA |
WO2020047124A1 (en) | 2018-08-28 | 2020-03-05 | Flagship Pioneering, Inc. | Methods and compositions for modulating a genome |
KR102298105B1 (ko) | 2019-01-08 | 2021-09-03 | 주식회사 엘지에너지솔루션 | 스웰링 게이지를 구비한 배터리 모듈 및 이를 포함하는 배터리 팩 |
SG11202109679VA (en) | 2019-03-19 | 2021-10-28 | Broad Inst Inc | Methods and compositions for editing nucleotide sequences |
WO2021175756A1 (en) | 2020-03-04 | 2021-09-10 | Basf Se | Shuttle vector for expression in e. coli and bacilli |
CN116209770A (zh) | 2020-03-04 | 2023-06-02 | 旗舰先锋创新Vi有限责任公司 | 用于调控基因组的改善的方法和组合物 |
KR20220150328A (ko) | 2020-03-04 | 2022-11-10 | 바스프 에스이 | 낮은 내지 중간 발현을 부여하는 구성적 박테리아 프로모터를 생산하는 방법 |
MX2022014008A (es) | 2020-05-08 | 2023-02-09 | Broad Inst Inc | Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo. |
CN112111505B (zh) * | 2020-09-17 | 2022-03-15 | 江南大学 | 一种氧化葡萄糖酸杆菌中基因敲除的方法 |
WO2022251179A1 (en) * | 2021-05-26 | 2022-12-01 | Inscripta, Inc. | Crispr editing in diploid genomes |
EP4384616A2 (en) * | 2021-08-11 | 2024-06-19 | The Board of Trustees of the Leland Stanford Junior University | High-throughput precision genome editing in human cells |
WO2023064928A2 (en) | 2021-10-14 | 2023-04-20 | Arsenal Biosciences, Inc. | Immune cells having co-expressed shrnas and logic gate systems |
WO2023114872A2 (en) * | 2021-12-14 | 2023-06-22 | The Broad Institute, Inc. | Reprogrammable fanzor polynucleotides and uses thereof |
AU2023228036A1 (en) | 2022-03-01 | 2024-09-12 | BASF Agricultural Solutions Seed US LLC | Cas12a nickases |
CN114774420B (zh) * | 2022-04-27 | 2023-09-22 | 扬州大学 | 一种水稻增强子及基于CRISPR/Cas9技术评估水稻增强子活性的方法 |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995015972A1 (en) | 1993-12-09 | 1995-06-15 | Thomas Jefferson University | Compounds and methods for site-directed mutations in eukaryotic cells |
US6027945A (en) | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
US6555732B1 (en) | 1998-09-14 | 2003-04-29 | Pioneer Hi-Bred International, Inc. | Rac-like genes and methods of use |
EP1152058A1 (en) | 2000-05-03 | 2001-11-07 | Institut Curie | Methods and compositions for effecting homologous recombination |
DK2336362T3 (en) | 2005-08-26 | 2019-01-21 | Dupont Nutrition Biosci Aps | USE OF CRISPR-ASSOCIATED GENES (CAS) |
US20140315985A1 (en) | 2013-03-14 | 2014-10-23 | Caribou Biosciences, Inc. | Compositions and methods of nucleic acid-targeting nucleic acids |
SG10201710488TA (en) | 2013-06-17 | 2018-01-30 | Broad Inst Inc | Delivery, Engineering and Optimization of Systems, Methods and Compositions for Targeting and Modeling Diseases and Disorders of Post Mitotic Cells |
US10563225B2 (en) * | 2013-07-26 | 2020-02-18 | President And Fellows Of Harvard College | Genome engineering |
US10392630B2 (en) | 2013-08-12 | 2019-08-27 | BASF Agro B.V. | Plants having increased tolerance to herbicides |
AU2014369229A1 (en) | 2013-12-18 | 2016-06-09 | BASF Agro B.V. | Plants having increased tolerance to herbicides |
CA2951707A1 (en) | 2014-06-10 | 2015-12-17 | Massachusetts Institute Of Technology | Method for gene editing |
WO2016065364A1 (en) * | 2014-10-24 | 2016-04-28 | Life Technologies Corporation | Compositions and methods for enhancing homologous recombination |
EP3294879A4 (en) * | 2015-05-14 | 2019-02-20 | University of Southern California | OPTIMIZED GENETIZATION WITH A RECOMBINANT ENDONUCLEASE SYSTEM |
WO2017024047A1 (en) | 2015-08-03 | 2017-02-09 | Emendobio Inc. | Compositions and methods for increasing nuclease induced recombination rate in cells |
AU2016339053A1 (en) * | 2015-09-24 | 2018-04-12 | Crispr Therapeutics Ag | Novel family of RNA-programmable endonucleases and their uses in genome editing and other applications |
WO2017059241A1 (en) | 2015-10-02 | 2017-04-06 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Lentiviral protein delivery system for rna-guided genome editing |
CN106811479B (zh) | 2015-11-30 | 2019-10-25 | 中国农业科学院作物科学研究所 | 利用CRISPR/Cas9系统定点修饰ALS基因获得抗除草剂水稻的系统及其应用 |
EP3443088B1 (en) * | 2016-04-13 | 2024-09-18 | Editas Medicine, Inc. | Grna fusion molecules, gene editing systems, and methods of use thereof |
EP3541945A4 (en) | 2016-11-18 | 2020-12-09 | Genedit Inc. | COMPOSITIONS AND METHODS OF MODIFICATION OF TARGET NUCLEIC ACIDS |
-
2017
- 2017-04-20 CN CN201780025990.6A patent/CN109072207B/zh active Active
- 2017-04-20 WO PCT/EP2017/059331 patent/WO2017186550A1/en active Application Filing
- 2017-04-20 EP EP22193652.9A patent/EP4166660A1/en not_active Withdrawn
- 2017-04-20 MX MX2018013256A patent/MX2018013256A/es unknown
- 2017-04-20 KR KR1020187030717A patent/KR102370675B1/ko active IP Right Grant
- 2017-04-20 EP EP22193659.4A patent/EP4166662A1/en not_active Withdrawn
- 2017-04-20 EP EP17720040.9A patent/EP3448990B1/en not_active Revoked
- 2017-04-20 DK DK17720040.9T patent/DK3448990T5/da active
- 2017-04-20 KR KR1020227006922A patent/KR102506185B1/ko active IP Right Grant
- 2017-04-20 AU AU2017256517A patent/AU2017256517B2/en active Active
- 2017-04-20 KR KR1020237007132A patent/KR20230038804A/ko not_active Application Discontinuation
- 2017-04-20 JP JP2018555963A patent/JP7184648B2/ja active Active
- 2017-04-20 US US16/097,300 patent/US11608499B2/en active Active
- 2017-04-20 BR BR112018068934A patent/BR112018068934A2/pt unknown
- 2017-04-20 EP EP22193654.5A patent/EP4166661A1/en active Pending
- 2017-04-20 EP EP21162462.2A patent/EP3868880A1/en active Pending
- 2017-04-20 CA CA3022475A patent/CA3022475A1/en active Pending
-
2018
- 2018-10-16 IL IL262409A patent/IL262409A/en unknown
- 2018-10-29 MX MX2021009235A patent/MX2021009235A/es unknown
- 2018-10-29 CL CL2018003074A patent/CL2018003074A1/es unknown
-
2022
- 2022-09-16 US US17/933,015 patent/US20230049124A1/en active Pending
- 2022-09-16 US US17/933,003 patent/US20230024869A1/en active Pending
- 2022-09-16 US US17/933,023 patent/US20230107997A1/en active Pending
- 2022-09-16 US US17/933,005 patent/US20230183686A1/en active Pending
- 2022-11-04 AU AU2022100158A patent/AU2022100158A4/en active Active
- 2022-11-04 AU AU2022100155A patent/AU2022100155A4/en active Active
- 2022-11-04 AU AU2022100157A patent/AU2022100157A4/en active Active
- 2022-11-14 AU AU2022100170A patent/AU2022100170A4/en active Active
- 2022-11-24 JP JP2022187824A patent/JP2023018093A/ja active Pending
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
BR112018068934A2 (pt) | método de modificação de moléculas de ácido nucleico alvo (na alvo) em células, molécula, molécula de funa recombinante, vetor e seu uso, sistema de vetor, sistema de modificação de na alvo em células, composição | |
WO2018015936A3 (en) | Methods and compositions for modifying genomic dna | |
BR112019006384A2 (pt) | enzimas modificadoras de ácido nucleico guiadas por rna e métodos de uso das mesmas | |
ES2690386T3 (es) | Composición para escindir un ADN diana que comprende un ARN guía específico para el ADN diana y el ácido nucleico que codifica la proteína Cas o la propia proteína Cas, y sus usos | |
CY1120538T1 (el) | Τροποποιημενες ριβονουκλεοπρωτεϊνες cascade και χρησεις αυτων | |
EA201892810A1 (ru) | Гибридные последовательности нуклеиновых кислот для геномной инженерии | |
BR112015030491A8 (pt) | método de modular a expressão de um ácido nucleico alvo em uma célula, bem como método de alterar um ácido nucleico de dna alvo em uma célula | |
BR112015004848B8 (pt) | Método para produção de uma célula de planta transgênica | |
UA120923C2 (uk) | Молекула нуклеїнової кислоти, яка кодує капсидний протеїн aav5 | |
WO2017070632A3 (en) | Nucleobase editors and uses thereof | |
EA201490056A1 (ru) | Способы и композиции для получения растений, обладающих мужской стерильностью | |
BR112022002695A2 (pt) | Nucleases guiadas por rna, fragmentos ativos e variantes das mesmas e métodos de uso | |
BR112019011582A2 (pt) | anticorpos e métodos de utilização dos mesmos | |
SG10201807736SA (en) | Composition and method for stabilizing nucleic acids in biological samples | |
BR112018010681A8 (pt) | método para modificar um sítio alvejado, complexo de enzima de modificação de ácido nucleico, e, ácido nucleico. | |
MX2018009251A (es) | Tubo fragmentador de microesferas y metodo para extraer acido desoxirribonucleico y/o acido ribonucleico de microorganismos. | |
WO2015160895A3 (en) | Modified transposases for improved insertion sequence bias and increased dna input tolerance | |
HRP20211882T1 (hr) | Protutijela protiv cd40 i njihova upotreba | |
AR085825A1 (es) | Evento de algodon transgenico mon 88701 y sus metodos de uso | |
BR112015004864A2 (pt) | enriquecimento de classificação de células ativadas por fluorescência (facs) para gerar plantas | |
EA201590488A1 (ru) | Способы модификации клеток-хозяев | |
AR088263A1 (es) | Moleculas de acido nucleico que se dirigen a rps6 y confieren resistencia a plagas de coleopteros | |
CY1123363T1 (el) | Απομονωση νουκλεϊνικων οξεων | |
BR112014022467A2 (pt) | transformantes de rasamsonia | |
SG11201809242VA (en) | Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
B350 | Update of information on the portal [chapter 15.35 patent gazette] | ||
B06W | Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette] | ||
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] |