MX2018009251A - Tubo fragmentador de microesferas y metodo para extraer acido desoxirribonucleico y/o acido ribonucleico de microorganismos. - Google Patents

Tubo fragmentador de microesferas y metodo para extraer acido desoxirribonucleico y/o acido ribonucleico de microorganismos.

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Publication number
MX2018009251A
MX2018009251A MX2018009251A MX2018009251A MX2018009251A MX 2018009251 A MX2018009251 A MX 2018009251A MX 2018009251 A MX2018009251 A MX 2018009251A MX 2018009251 A MX2018009251 A MX 2018009251A MX 2018009251 A MX2018009251 A MX 2018009251A
Authority
MX
Mexico
Prior art keywords
bead beating
microorganisms
acid
beating tube
extracting deoxyribonucleic
Prior art date
Application number
MX2018009251A
Other languages
English (en)
Inventor
Klapproth Holger
Smit Nicolaas
Original Assignee
Safeguard Biosystems Holdings Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Safeguard Biosystems Holdings Ltd filed Critical Safeguard Biosystems Holdings Ltd
Publication of MX2018009251A publication Critical patent/MX2018009251A/es

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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/02—Apparatus for enzymology or microbiology with agitation means; with heat exchange means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/303—Applying a physical force on a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sustainable Development (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Prostheses (AREA)
  • Information Retrieval, Db Structures And Fs Structures Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La presente descripción proporciona métodos mejorados para fragmentado de microesferas y un sistema de fragmentado de microesferas útil del mismo. El sistema de fragmentado de microesferas comprende un tubo de muestra, microesferas, y un agente de bloqueo seco, y métodos para utilizar el sistema de fragmentado de microesferas para extraer ácidos nucleicos de células que contienen los ácidos nucleicos.
MX2018009251A 2016-01-30 2017-01-30 Tubo fragmentador de microesferas y metodo para extraer acido desoxirribonucleico y/o acido ribonucleico de microorganismos. MX2018009251A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP16153540.6A EP3199629A1 (en) 2016-01-30 2016-01-30 Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
PCT/EP2017/051902 WO2017129814A1 (en) 2016-01-30 2017-01-30 Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms

Publications (1)

Publication Number Publication Date
MX2018009251A true MX2018009251A (es) 2018-11-29

Family

ID=55310672

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018009251A MX2018009251A (es) 2016-01-30 2017-01-30 Tubo fragmentador de microesferas y metodo para extraer acido desoxirribonucleico y/o acido ribonucleico de microorganismos.

Country Status (16)

Country Link
EP (1) EP3199629A1 (es)
JP (2) JP2019503193A (es)
KR (2) KR20200121912A (es)
CN (2) CN108699550B (es)
AU (1) AU2017211983B2 (es)
BR (1) BR112018014865A2 (es)
CA (1) CA3011642A1 (es)
IL (2) IL260800B (es)
MX (1) MX2018009251A (es)
NZ (1) NZ744436A (es)
PH (1) PH12018501507A1 (es)
RU (1) RU2743140C2 (es)
SG (1) SG11201806094SA (es)
TW (1) TWI747876B (es)
WO (1) WO2017129814A1 (es)
ZA (1) ZA201804830B (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
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US10036054B2 (en) 2016-01-30 2018-07-31 Safeguard Biosystems Holdings Ltd. Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
EP3573756A1 (en) * 2017-01-30 2019-12-04 Safeguard Biosystems Holdings Ltd. Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
CN107365766B (zh) * 2017-08-04 2020-10-02 中国农业大学 机械破碎法提取霉菌孢子rna的方法
CN107475242B (zh) * 2017-08-04 2020-07-28 中国农业大学 机械破碎法提取霉菌孢子dna的方法
CN109870574A (zh) * 2017-12-05 2019-06-11 中国科学院上海生命科学研究院 提取食品中抗生素残留的试剂盒以及检测方法
KR102117833B1 (ko) * 2019-01-04 2020-06-02 남장희 생물시료로부터 유전물질을 신속하게 분리하는 핵산 흡착 물질을 포함하는 조성물 및 그를 이용하여 생물시료로부터 유전물질을 신속하게 분리하는 방법
CN113073096B (zh) * 2021-03-31 2023-02-03 北京源微生物科技有限公司 一种血液中病原微生物分离、富集和核酸提取方法及试剂
CN112921031A (zh) * 2021-04-20 2021-06-08 深圳职业技术学院 一种土壤微生物总dna高效提取方法
CN114292841B (zh) * 2021-12-30 2023-06-09 苏州白垩纪生物科技有限公司 核酸提取试剂和核酸提取方法
GB202207989D0 (en) * 2022-05-30 2022-07-13 Guys And St Thomas Nhs Found Trust Metagenomics
CN116286798B (zh) * 2023-03-29 2024-04-02 杭州创新生物检控技术有限公司 一种适用于细菌dna提取纯化的试剂盒及方法

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Also Published As

Publication number Publication date
KR20200121912A (ko) 2020-10-26
RU2743140C2 (ru) 2021-02-15
CN108699550A (zh) 2018-10-23
AU2017211983B2 (en) 2023-01-05
KR20180114909A (ko) 2018-10-19
TW201726924A (zh) 2017-08-01
CA3011642A1 (en) 2017-08-03
IL291231A (en) 2022-05-01
IL260800B (en) 2022-04-01
CN116694620A (zh) 2023-09-05
JP2019503193A (ja) 2019-02-07
RU2018130749A (ru) 2020-03-02
TWI747876B (zh) 2021-12-01
TW202210623A (zh) 2022-03-16
JP2022033906A (ja) 2022-03-02
WO2017129814A1 (en) 2017-08-03
AU2017211983A1 (en) 2018-08-09
BR112018014865A2 (pt) 2018-12-18
CN108699550B (zh) 2023-05-12
NZ744436A (en) 2020-10-30
JP7488246B2 (ja) 2024-05-21
EP3199629A1 (en) 2017-08-02
RU2018130749A3 (es) 2020-04-09
PH12018501507A1 (en) 2019-04-08
SG11201806094SA (en) 2018-08-30
ZA201804830B (en) 2021-03-31

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