SG11201806094SA - Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms - Google Patents

Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms

Info

Publication number
SG11201806094SA
SG11201806094SA SG11201806094SA SG11201806094SA SG11201806094SA SG 11201806094S A SG11201806094S A SG 11201806094SA SG 11201806094S A SG11201806094S A SG 11201806094SA SG 11201806094S A SG11201806094S A SG 11201806094SA SG 11201806094S A SG11201806094S A SG 11201806094SA
Authority
SG
Singapore
Prior art keywords
international
bead beating
acid
microorganisms
huwer
Prior art date
Application number
SG11201806094SA
Other languages
English (en)
Inventor
Holger Klapproth
Nicolaas Smit
Original Assignee
Safeguard Biosystems Holdings Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Safeguard Biosystems Holdings Ltd filed Critical Safeguard Biosystems Holdings Ltd
Publication of SG11201806094SA publication Critical patent/SG11201806094SA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/02Apparatus for enzymology or microbiology with agitation means; with heat exchange means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082Test tubes per se
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/08Flask, bottle or test tube
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • C12N1/066Lysis of microorganisms by physical methods
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/041Connecting closures to device or container
    • B01L2300/042Caps; Plugs
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00Reactions characterised by treatment of reaction samples
    • C12Q2523/30Characterised by physical treatment
    • C12Q2523/303Applying a physical force on a nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/125Specific component of sample, medium or buffer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/137Concentration of a component of medium

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Sustainable Development (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Prostheses (AREA)
  • Information Retrieval, Db Structures And Fs Structures Therefor (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SG11201806094SA 2016-01-30 2017-01-30 Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms SG11201806094SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP16153540.6A EP3199629A1 (en) 2016-01-30 2016-01-30 Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
PCT/EP2017/051902 WO2017129814A1 (en) 2016-01-30 2017-01-30 Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms

Publications (1)

Publication Number Publication Date
SG11201806094SA true SG11201806094SA (en) 2018-08-30

Family

ID=55310672

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201806094SA SG11201806094SA (en) 2016-01-30 2017-01-30 Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms

Country Status (16)

Country Link
EP (1) EP3199629A1 (es)
JP (2) JP2019503193A (es)
KR (2) KR20200121912A (es)
CN (2) CN108699550B (es)
AU (1) AU2017211983B2 (es)
BR (1) BR112018014865A2 (es)
CA (1) CA3011642A1 (es)
IL (2) IL260800B (es)
MX (1) MX2018009251A (es)
NZ (1) NZ744436A (es)
PH (1) PH12018501507A1 (es)
RU (1) RU2743140C2 (es)
SG (1) SG11201806094SA (es)
TW (1) TWI747876B (es)
WO (1) WO2017129814A1 (es)
ZA (1) ZA201804830B (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10036054B2 (en) 2016-01-30 2018-07-31 Safeguard Biosystems Holdings Ltd. Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
EP3573756A1 (en) * 2017-01-30 2019-12-04 Safeguard Biosystems Holdings Ltd. Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
CN107365766B (zh) * 2017-08-04 2020-10-02 中国农业大学 机械破碎法提取霉菌孢子rna的方法
CN107475242B (zh) * 2017-08-04 2020-07-28 中国农业大学 机械破碎法提取霉菌孢子dna的方法
CN109870574A (zh) * 2017-12-05 2019-06-11 中国科学院上海生命科学研究院 提取食品中抗生素残留的试剂盒以及检测方法
KR102117833B1 (ko) * 2019-01-04 2020-06-02 남장희 생물시료로부터 유전물질을 신속하게 분리하는 핵산 흡착 물질을 포함하는 조성물 및 그를 이용하여 생물시료로부터 유전물질을 신속하게 분리하는 방법
CN113073096B (zh) * 2021-03-31 2023-02-03 北京源微生物科技有限公司 一种血液中病原微生物分离、富集和核酸提取方法及试剂
CN112921031A (zh) * 2021-04-20 2021-06-08 深圳职业技术学院 一种土壤微生物总dna高效提取方法
CN114292841B (zh) * 2021-12-30 2023-06-09 苏州白垩纪生物科技有限公司 核酸提取试剂和核酸提取方法
GB202207989D0 (en) * 2022-05-30 2022-07-13 Guys And St Thomas Nhs Found Trust Metagenomics
CN116286798B (zh) * 2023-03-29 2024-04-02 杭州创新生物检控技术有限公司 一种适用于细菌dna提取纯化的试剂盒及方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69528256T2 (de) * 1994-04-14 2003-07-31 Rockefeller University New Yor Vorrichtung zur isolierung von zellularinhaltsstoffen
SE9904539D0 (sv) * 1999-12-10 1999-12-10 Alphahelix Ab Method and device for the handling of samples and reagents
US7494771B2 (en) * 2001-07-19 2009-02-24 Geneohm Sciences Canada, Inc. Universal method and composition for the rapid lysis of cells for the release of nucleic acids and their detection
CN101163802A (zh) * 2005-04-19 2008-04-16 杜邦公司 通过靶基因组的分级分离增加基于核酸的生物体检测的灵敏性
JP2008154521A (ja) * 2006-12-25 2008-07-10 Kurita Water Ind Ltd 塩素化エチレン分解菌の塩化ビニル分解能の判定方法、及び該方法に用いられる塩素化エチレン分解菌の塩化ビニル分解能判定用キット
JP4522434B2 (ja) * 2007-05-31 2010-08-11 キヤノン株式会社 血液採取用容器
WO2009135158A2 (en) * 2008-05-02 2009-11-05 Immunetics, Inc. Detection of microbial nucleic acids
WO2010029609A1 (ja) * 2008-09-09 2010-03-18 サントリーホールディングス株式会社 グルコース誘導性不活化/分解耐性トランスポーター遺伝子及びその用途
US20100137575A1 (en) * 2008-12-03 2010-06-03 Connolly D Michael Universal biological sample processing
EP2210936A1 (en) * 2009-01-27 2010-07-28 Curetis AG Processing and analysis of viscous liquid biological samples
EP2443251B1 (en) * 2009-06-15 2014-08-13 De Nationale Geologiske Undersøgelser For Danmak Og Grønland Improvement of low-biomass soil dna/rna extraction yield and quality
WO2012027302A2 (en) * 2010-08-21 2012-03-01 The Regents Of The University Of California Systems and methods for detecting antibiotic resistance
US10041061B2 (en) * 2010-09-29 2018-08-07 Ibis Biosciences, Inc. Fungal nucleic acid extraction
BR122020001802B1 (pt) * 2010-10-22 2021-05-18 T2 Biosystems, Inc métodos para detectar a presença de um patógeno em uma amostra de sangue total e para detectar um analito em uma amostra
US20150031038A1 (en) * 2011-09-06 2015-01-29 Ibis Biosciences, Inc. Sample preparation methods
WO2013091102A1 (en) * 2011-12-21 2013-06-27 Geneohm Sciences Canada Inc. Enrichment & isolation of microbial cells & microbial nucleic acids from a biological sample
US9334491B2 (en) * 2011-12-22 2016-05-10 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
CA2868805C (en) * 2012-03-28 2018-09-04 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for the collection and isolation of nucleic acids from biological specimens
EP3017042A4 (en) * 2013-07-02 2017-02-22 Ibis Biosciences, Inc. Method for the purification of targeted nucleic acids from background nucleic acids
WO2015154189A1 (en) * 2014-04-10 2015-10-15 Dna Genotek Inc. Method and system for microbial lysis using periodates

Also Published As

Publication number Publication date
KR20200121912A (ko) 2020-10-26
RU2743140C2 (ru) 2021-02-15
CN108699550A (zh) 2018-10-23
AU2017211983B2 (en) 2023-01-05
KR20180114909A (ko) 2018-10-19
TW201726924A (zh) 2017-08-01
CA3011642A1 (en) 2017-08-03
IL291231A (en) 2022-05-01
IL260800B (en) 2022-04-01
CN116694620A (zh) 2023-09-05
JP2019503193A (ja) 2019-02-07
RU2018130749A (ru) 2020-03-02
TWI747876B (zh) 2021-12-01
TW202210623A (zh) 2022-03-16
JP2022033906A (ja) 2022-03-02
WO2017129814A1 (en) 2017-08-03
AU2017211983A1 (en) 2018-08-09
BR112018014865A2 (pt) 2018-12-18
MX2018009251A (es) 2018-11-29
CN108699550B (zh) 2023-05-12
NZ744436A (en) 2020-10-30
JP7488246B2 (ja) 2024-05-21
EP3199629A1 (en) 2017-08-02
RU2018130749A3 (es) 2020-04-09
PH12018501507A1 (en) 2019-04-08
ZA201804830B (en) 2021-03-31

Similar Documents

Publication Publication Date Title
SG11201806094SA (en) Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
SG11201811048UA (en) Single-cell transcript sequencing
SG11201903480TA (en) Cell culture device system and methods of use thereof
SG11201806663TA (en) Closed-ended linear duplex dna for non-viral gene transfer
SG11201805805VA (en) Recombinogenic nucleic acid strands in situ
SG11201810003UA (en) Using programmable dna binding proteins to enhance targeted genome modification
SG11201809522WA (en) Method of nucleic acid sequence determination
SG11201811431VA (en) Multispecific antibodies against cd40 and cd137
SG11201804916PA (en) Three-dimensional polymer networks with channels situated therein
SG11201809912UA (en) Hybrid carriers for nucleic acid cargo
SG11201808721YA (en) Cell-free production of ribonucleic acid
SG11201906950PA (en) Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
SG11201900447SA (en) Methods and compositions for modifying genomic dna
SG11201900038QA (en) Compositions and methods comprising improvements of crispr guide rnas using the h1 promoter
SG11201901797RA (en) Solid electrolyte
SG11201807069XA (en) Nucleic acid sequencing method and system employing enhanced detection of nucleotide-specific ternary complex formation
SG11201908675PA (en) Computer-implemented system and method for performing transaction mixing on a blockchain
SG11201811149TA (en) METABOLIC DRUG LOADING OF EVs
SG11201900753VA (en) A method of isolating mesenchymal stem cells from umbilical cord amniotic membrane using a cell culture medium
SG11201911572YA (en) Adenoviral vectors
SG11201805898RA (en) Liquid composition comprising a multistage polymer, its method of preparation and its use
SG11201903329WA (en) Method for preparing electrocompetent yeast cells, and method for using said cells
SG11201806400XA (en) Replicative transposon system
SG11201904535VA (en) Prevention and treatment of bone and cartilage damage or disease
SG11201808762XA (en) Diagnostic methods for patient specific therapeutic decision making in cancer care