SG11201900753VA - A method of isolating mesenchymal stem cells from umbilical cord amniotic membrane using a cell culture medium - Google Patents
A method of isolating mesenchymal stem cells from umbilical cord amniotic membrane using a cell culture mediumInfo
- Publication number
- SG11201900753VA SG11201900753VA SG11201900753VA SG11201900753VA SG11201900753VA SG 11201900753V A SG11201900753V A SG 11201900753VA SG 11201900753V A SG11201900753V A SG 11201900753VA SG 11201900753V A SG11201900753V A SG 11201900753VA SG 11201900753V A SG11201900753V A SG 11201900753VA
- Authority
- SG
- Singapore
- Prior art keywords
- international
- umbilical cord
- mesenchymal stem
- amniotic membrane
- culture medium
- Prior art date
Links
- 210000003954 umbilical cord Anatomy 0.000 title abstract 5
- 210000001691 amnion Anatomy 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 4
- 210000002901 mesenchymal stem cell Anatomy 0.000 title abstract 3
- 239000006143 cell culture medium Substances 0.000 title abstract 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 abstract 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 abstract 2
- 239000012091 fetal bovine serum Substances 0.000 abstract 2
- 239000002609 medium Substances 0.000 abstract 2
- 102100022464 5'-nucleotidase Human genes 0.000 abstract 1
- 101100163869 Arabidopsis thaliana AS1 gene Proteins 0.000 abstract 1
- 102100037241 Endoglin Human genes 0.000 abstract 1
- 102000006354 HLA-DR Antigens Human genes 0.000 abstract 1
- 108010058597 HLA-DR Antigens Proteins 0.000 abstract 1
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 abstract 1
- 101000678236 Homo sapiens 5'-nucleotidase Proteins 0.000 abstract 1
- 101000881679 Homo sapiens Endoglin Proteins 0.000 abstract 1
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 abstract 1
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 abstract 1
- 101000800116 Homo sapiens Thy-1 membrane glycoprotein Proteins 0.000 abstract 1
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 abstract 1
- 102100033523 Thy-1 membrane glycoprotein Human genes 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 230000008520 organization Effects 0.000 abstract 1
- 239000008194 pharmaceutical composition Substances 0.000 abstract 1
- 210000000130 stem cell Anatomy 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0668—Mesenchymal stem cells from other natural sources
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/34—Sugars
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/40—Nucleotides, nucleosides, bases
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/80—Undefined extracts from animals
- C12N2500/84—Undefined extracts from animals from mammals
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/33—Insulin
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/395—Thyroid hormones
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
- C12N2506/025—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells from extra-embryonic cells, e.g. trophoblast, placenta
Abstract
INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 12 April 2018 (12.04.2018) WIP0 1 PCT °mons °nolo omoiloo mon ilommio oimIE (10) International Publication Number WO 2018/067071 Al (51) International Patent Classification: C12N 5/0775 (2010.01) A61K 35/51 (2015.01) (21) International Application Number: PCT/SG2017/050500 (22) International Filing Date: 05 October 2017 (05.10.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/404,582 05 October 2016 (05.10.2016) US (71) Applicant: CELLRESEARCH CORPORATION PTE. LTD. [SG/SG]; 137 Market Street, #08-02, Grace Global Raffles Building, Singapore 048943 (SG). (72) Inventor: PHAN, Toan Thang; 137 Market Street, #08-02, Grace Global Raffles Building, Singapore 048943 (SG). (74) Agent: MCLAUGHLIN, Michael Gerard; McLaughlin IP Pte. Ltd., 24A Mosque Street, Singapore 059504 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, = (54) Title: A METHOD OF ISOLATING MESENCHYMAL STEM CELLS FROM UMBILICAL CORD AMNIOTIC MEMBRANE USING A CELL CULTURE MEDIUM 99,70% 99,80% 139,50% 99,00% 99,70% 99,50% 1-1 1-1 N O N O GC O 1-1 O Fig. 6c (57) : The present invention relates to a method of isolating a mesenchymal stem cell population from the amniotic membrane of the umbilical cord, the method comprising cultivating umbilical cord tissue in a culture medium comprising DMEM (Dulbecco's modified eagle medium), F12 (Ham's F12 Medium), M171 (Medium 171) and FBS (Fetal Bovine Serum). The invention also relates to a mesenchymal stem population isolated from the amniotic membrane of the umbilical cord, wherein at least about 90 % or more cells of the stem cell population express each of the following markers: CD73, CD90 and CD105 and lack expression of the following markers: CD34, CD45 and HLA-DR. The invention also relates to a pharmaceutical composition of this mesenchymal stem population. [Continued on next page] WO 2018/067071 Al MIDEDIMOMOIDEIROHOINII01100111011EIHIEMOVOIS MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h))
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662404582P | 2016-10-05 | 2016-10-05 | |
| PCT/SG2017/050500 WO2018067071A1 (en) | 2016-10-05 | 2017-10-05 | A method of isolating mesenchymal stem cells from umbilical cord amniotic membrane using a cell culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201900753VA true SG11201900753VA (en) | 2019-02-27 |
Family
ID=61832065
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201900753VA SG11201900753VA (en) | 2016-10-05 | 2017-10-05 | A method of isolating mesenchymal stem cells from umbilical cord amniotic membrane using a cell culture medium |
Country Status (18)
| Country | Link |
|---|---|
| US (2) | US10988736B2 (en) |
| EP (1) | EP3523421A4 (en) |
| JP (2) | JP7262775B2 (en) |
| KR (1) | KR102491558B1 (en) |
| CN (1) | CN109804063A (en) |
| AU (2) | AU2017339344B2 (en) |
| BR (1) | BR112019005652A2 (en) |
| CA (1) | CA3031978A1 (en) |
| CL (1) | CL2019000540A1 (en) |
| CO (1) | CO2019002569A2 (en) |
| IL (2) | IL264197B2 (en) |
| MX (1) | MX2019003936A (en) |
| PH (1) | PH12019500187A1 (en) |
| SA (1) | SA519401455B1 (en) |
| SG (1) | SG11201900753VA (en) |
| TW (1) | TWI773693B (en) |
| WO (1) | WO2018067071A1 (en) |
| ZA (1) | ZA201900207B (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101316602B (en) | 2005-09-27 | 2015-06-03 | 组织技术公司 | Amniotic membrane preparations and purified compositions and methods of use |
| EP2872181B1 (en) | 2012-07-11 | 2020-11-18 | Tissuetech, Inc. | Compositions containing hc-ha/ptx3 complexes and methods of use thereof |
| WO2016138025A2 (en) | 2015-02-23 | 2016-09-01 | Tissuetech, Inc. | Apparatuses and methods for treating ophthalmic diseases and disorders |
| JP2018516869A (en) | 2015-05-20 | 2018-06-28 | ティッシュテック，インク． | Compositions and methods for preventing epithelial cell proliferation and epithelial-mesenchymal transition |
| TW201733600A (en) | 2016-01-29 | 2017-10-01 | 帝聖工業公司 | Fetal support tissue products and methods of use |
| BR112019005652A2 (en) * | 2016-10-05 | 2019-06-04 | Cellresearch Corp Pte Ltd | method of isolating mesenchymal stem cells from the umbilical cord amniotic membrane using a cell culture medium |
| KR102015502B1 (en) * | 2018-03-29 | 2019-08-28 | 아키소스템바이오스트래티지스(주) | A method for the isolation of stem cells from human umbilical cords |
| WO2019199230A1 (en) * | 2018-04-09 | 2019-10-17 | Cellresearch Corporation Pte. Ltd. | A method of transporting mesenchymal stem cells by means of a cell culture medium and a method of administering stem cells to wounds |
| EP3772936A4 (en) | 2018-04-09 | 2022-01-05 | CellResearch Corporation Pte. Ltd. | A method of transporting mesenchymal stem cells by means of a transporting solution and a method of administering stem cells to wounds |
| TW202012618A (en) * | 2018-04-12 | 2020-04-01 | 新加坡商細胞研究私人有限公司 | A method of inducing or improving wound healing properties of mesenchymal stem cells |
| US20210324335A1 (en) * | 2018-09-04 | 2021-10-21 | Academia Sinica | Medium composition and method for culturing mesenchymal stem cells |
| AU2020362379A1 (en) * | 2019-10-08 | 2022-04-07 | Cellresearch Corporation Pte. Ltd. | A method of assessing wound healing potency of a mesenchymal stem population and related methods of selecting mesenchymal stem cells and identifying tissue as starting material for producing a mesenchymal stem cell population |
| CN114867347B (en) * | 2019-10-08 | 2024-03-05 | 细胞研究私人有限公司 | Storage or transport formulations of mesenchymal stem cells and methods of making and using the same |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL1789534T3 (en) | 2004-08-16 | 2015-03-31 | Cellresearch Corp Pte Ltd | Isolation of stem/progenitor cells from amniotic membrane of umbilical cord |
| WO2007046775A1 (en) | 2005-10-21 | 2007-04-26 | Cellresearch Corporation Pte Ltd | Isolation and cultivation of stem/progenitor cells from the amniotic membrane of umbilical cord and uses of cells differentiated therefrom |
| CN101525594B (en) * | 2009-04-17 | 2011-04-20 | 中国医学科学院血液学研究所 | Complete medium with low serum concentration for cultivating mesenchymal stem cells and method for cultivating mesenchymal stem cells using same |
| CN103266081B (en) * | 2012-01-21 | 2015-05-13 | 中国人民解放军军事医学科学院附属医院 | Efficient method for isolating and culturing mesenchymal stem cells from umbilical cord |
| US8940294B2 (en) * | 2012-03-02 | 2015-01-27 | Tissuetech, Inc. | Methods of isolating and culturing stem cells |
| CN103013917A (en) * | 2012-11-30 | 2013-04-03 | 陆华 | Method for inducing human amniotic mesenchymal stem cells to differentiate into neuron-like cells |
| WO2014131877A1 (en) | 2013-03-01 | 2014-09-04 | Apceth Gmbh & Co. Kg | Protection of the vascular endothelium from immunologically mediated cytotoxic reactions with human cd34-negative progenitor cells |
| JP6745449B2 (en) | 2013-12-27 | 2020-08-26 | ゼノアックリソース株式会社 | Umbilical cord tissue cryopreservation method |
| CN105420184A (en) * | 2015-12-11 | 2016-03-23 | 郭镭 | Method for culturing umbilical cord mesenchymal stem cells in separated mode from umbilical cord outer layer amnion tissue |
| CN105420179A (en) * | 2015-12-17 | 2016-03-23 | 斯坦姆（天津）生物技术研究有限公司 | Method for simultaneously extracting epithelial cells and mesenchymal stem cells from umbilical cord and placenta amnion tissues |
| BR112019005652A2 (en) * | 2016-10-05 | 2019-06-04 | Cellresearch Corp Pte Ltd | method of isolating mesenchymal stem cells from the umbilical cord amniotic membrane using a cell culture medium |
| TW202002999A (en) * | 2018-04-06 | 2020-01-16 | 新加坡商細胞研究私人有限公司 | Use of an essentially pure mesenchymal stem cell population of the amniotic membrane of umbilical cord for generating a mammalian stem cell carrying a transgene |
| CN114867347B (en) * | 2019-10-08 | 2024-03-05 | 细胞研究私人有限公司 | Storage or transport formulations of mesenchymal stem cells and methods of making and using the same |
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2017
- 2017-10-05 BR BR112019005652A patent/BR112019005652A2/en unknown
- 2017-10-05 IL IL264197A patent/IL264197B2/en unknown
- 2017-10-05 JP JP2019515832A patent/JP7262775B2/en active Active
- 2017-10-05 US US15/725,913 patent/US10988736B2/en active Active
- 2017-10-05 WO PCT/SG2017/050500 patent/WO2018067071A1/en unknown
- 2017-10-05 AU AU2017339344A patent/AU2017339344B2/en active Active
- 2017-10-05 KR KR1020197004328A patent/KR102491558B1/en active IP Right Grant
- 2017-10-05 CN CN201780061207.1A patent/CN109804063A/en active Pending
- 2017-10-05 SG SG11201900753VA patent/SG11201900753VA/en unknown
- 2017-10-05 EP EP17858825.7A patent/EP3523421A4/en active Pending
- 2017-10-05 MX MX2019003936A patent/MX2019003936A/en unknown
- 2017-10-05 IL IL307825A patent/IL307825A/en unknown
- 2017-10-05 CA CA3031978A patent/CA3031978A1/en active Pending
- 2017-10-05 TW TW106134409A patent/TWI773693B/en active
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2019
- 2019-01-11 ZA ZA2019/00207A patent/ZA201900207B/en unknown
- 2019-01-25 PH PH12019500187A patent/PH12019500187A1/en unknown
- 2019-02-28 CL CL2019000540A patent/CL2019000540A1/en unknown
- 2019-03-19 CO CONC2019/0002569A patent/CO2019002569A2/en unknown
- 2019-03-31 SA SA519401455A patent/SA519401455B1/en unknown
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2021
- 2021-03-02 US US17/190,228 patent/US11821006B2/en active Active
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2022
- 2022-12-27 JP JP2022209799A patent/JP2023036882A/en active Pending
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2024
- 2024-01-18 AU AU2024200348A patent/AU2024200348A1/en active Pending
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